• Journal of Microbiology and Biotechnology
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• v.25 no.5
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• pp.732-737
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• 2015

We present here an in silico search of fungal sterol-esterase/lipase and bacterial depolymerase sequences from environmental metagenomes. Both enzyme types contain the α/β-hydrolase protein fold. Analysis of DNA conserved motifs, protein homology search, phylogenetic analysis, and protein 3D modeling have been used, and the efficiency of these screening strategies is discussed. The presence of bacterial genes in the metagenomes was higher than those from fungi, and the sequencing depth of the metagenomes seemed to be crucial to allow finding enough diversity of enzyme sequences. As a result, a novel putative PHA-depolymerase is described.

• Journal of the Korean Applied Science and Technology
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• v.3 no.2
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• pp.17-22
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• 1986

Compositional and quantitative changes of 4-desmethylsterols in soybean (Glycine max. cv. Gangrim) seed and pod at six stages of development from flowering were investigated. The content of 4-desmethyl-, 4-monomethyl- and 4,4-dimethylsterols in developing soybean seed and pod was found to vary between 0.16-0.35, 0.04-0.12 and 0.04-0.06%, respectively. The predominating 4-desmethylsterols were sitosterol, campesterol and stigmasterol. The proportion of sitosterol in seed decreased to 57.0% with maturation, while in pod increased up to 88.7%. ${\triangle}^{7,22,25}$ Stigmastatrienol estimated 7.5% was detected in the fully mature seed.

• Korean Journal of Pharmacognosy
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• v.18 no.3
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• pp.180-183
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• 1987

Pericarp of Zanthoxylum coreanum Nakai have been used as a crude medicine for the treatment of ozena, rheumatoid, nasal sinusitis, meno-xenia, dyspepsia, toothache, sore throat, pains in the limbs, etc. in Korea. A sterol fraction and two compounds, compound I and II were isolated from the roots, stems, leaves of Zanthoxylum coreanum Nakai. The sterol fraction was identified as a mixture of ${\beta}-sitosterol$ and campesterol. Compound I, colorless prismatic crystals, $C_{13]H_{13}NO_4$, mp. $175{\sim}177^{\circ}$, was proved to be skimmianine which is one of the alkaloids and compound II, $C_{14}H_{28}O_2$, mp. $53{\sim}55^{\circ}$, white powdered crystals was identified as myristic acid.

• Korean Journal of Pharmacognosy
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• v.10 no.1
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• pp.9-12
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• 1979

The Korean Euodia daniellii $H_{EMSLEY}$ seed oil of triglyceride composition were analysed by thinchrography. Responses of sterol, triglyceride, sterol ester and acetate on a thin layer chromatographic apparatus, which was equipped with a flame ionization detector were determined. It was found that thinchrography is very superior and convenient means for the estimation of seed oil triglyceride compositions. The triglyceride compositions were tripalmitin 7.3%, tristearin 1.8%, triolein 21.1%, trilinolein 32.9%, trilinolenin 33.4% and unknown 3.5% in the case of Euodia daniellii seed oil in Korea.

• Journal of the Korean Magnetic Resonance Society
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• v.14 no.1
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• pp.1-8
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• 2010

Four 5,8-epidioxy sterols were isolated from the marine sponge Plakortis simplex. Their structures were completely determined by an extensive NMR analysis and comparison with NMR data of similar compounds for absolute stereochemistry of the side chain. The compounds were assigned as 5,8-epidioxy- (24S)-ethylcholesta-6,22(E),25-trien-3-ol(1), 5,8-epidioxy-(24S)-methylcholesta- 6,22(E)-dien-3-ol(2), 5,8-epidioxycholesta-6,22(E)-dien-3-ol(3) and 5,8- epidioxycholesta-6-en-3-ol (4).

• Journal of the korean society of oceanography
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• v.37 no.2
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• pp.51-57
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• 2002

The ${\Delta}^5$ sterols were measured in be sediments of Jinhae Bay surrounded by industrial cities to assess the sewage-derived contamination. The total concentrations of total sterols ranged from 2.03 to 19.56 ${\mu}g/g$ dry wt. The principal sterol was cholesterol with a contribution of more than 50% to total sterols. Coprostanol, providing an indication of long-term sewage loads, was found in all sediment samples and the concentrations were 0.03-3.86 ${\mu}g/g$ dry wt, accounting for 1-26% to total sterols. The cluster analysis of sampling stations indicated that the sewage-derived contamination was localized in inner Masan Bay.

• Korean Journal of Pharmacognosy
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• v.25 no.4 s.99
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• pp.342-347
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• 1994

Soybean and beanpaste have been used as ore of the important food in Korea. Beanpaste is made from soybean(seed of Glycine max $M_{ERRIL}$, Leguminosae) by traditional fermentation. It was expected that the secondary metabolites might be formed by the action of some microorganisms or enzymes on the constituents primarily contained in soybean during manufacturing or storage of beanpaste. A systematic investigation on the chemical constituents was carried out. As a result, sterol glycoside named daucosterin, lipo-daucosterin have been isolated from the lipid fraction soybean and their structures elucidated basis of chemical and physicochemical evidences. A ratio of composition of the lipid constituents in soybean and beanpaste were estimated.

• Korean Journal of Pharmacognosy
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• v.22 no.2
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• pp.78-84
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• 1991

From the whole plants of Orostachys japonicus(Crassulaceae), fatty acid ester mixture, seco-A-triterpene mixture, glutinone, friedelin, ${\beta}-amyrin$, glutinol, epifridelanol, 1-hexatriacontanol, sterol mixture, steryl glucoside mixture were isolated and characterized by spectral data.

• Microbiology and Biotechnology Letters
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• v.18 no.1
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• pp.12-17
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• 1990

A mutant of Mycobacterium sp. has been isolated which is capable of degrading cholesterol and plant sterol to androst-4-ene-3, 17-dione and 9-hydroxyandrostene-3, 17-dione. Also this mutant hydroxylated the steroidal nucleus at the 9 $\alpha$ position. No ring degradation inhibitory agents are required for these processes.

• The Korean Journal of Mycology
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• v.8 no.1
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• pp.25-28
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• 1980

Attempts were made to investigate sterol components of Calvatia saccatum (Vahl.) Fr. which grows wildly in Korea. Its carpophores were collected in Gyeong Gi Province and extracted with chloroform and methanol. Two compounds were isolated from the extract and one of these compounds was identified as ergosterol by TLC, GLC and chemical tests.