• Title/Summary/Keyword: Sterilization methods

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Influence of Sterilization Methods on Atrazine Assimilation (멸균법이 atrazine의 분해과정에 미치는 영향)

  • 정규혁;오승민
    • Journal of Environmental Health Sciences
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    • v.23 no.1
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    • pp.1-7
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    • 1997
  • We investigated the influence of various known sterilization methods on atrazine assimilation. The present study was designed to investigate the effect of autoclaving, sodium azide and mercuric chloride treatment on the assimilation of atrazine in soil and sediment. The sterilization reactor treated with sodium azide resulted in $^{14}CO_2$ generation and atrazine was rapidly disappeared from reactor through chemical reaction with sodium azide. These findings seem to indicate that sodium azide sterilization is not recommended for atrazine studies. In sample reactors autoclaved or treated with mercuric chloride, $^{14}CO_2$ generation was not detected and most of the disappeared atrazine was found to exist as hydroxyatrazine. These results suggested that autoclaving or mercuric chloride treatment could be effective sterilization methods. However, chemical properties(pH and redox potential) of soil and sediment were altered by any of the sterilization methods applied. So it was suspected that these altered properties could affect distribution and mineralization of atrazine in soil and sediment. In addition, both autoclaving and mercuric chloride treatment have altered $K_d$ values of hydroxyatrazine more significantly than those of atrazine. Consequently, although autoclaving and mercuric chloride treatment are effective sterilization methods, one must be careful in using them in practice as these methods may cause chemical degradation of both of atrazine and its metabolites and changes in chemical properties of soil and sediment. In conclusion, careful assessment of sterilization methods must be made for the degradation studies of chemicals in soil and sediment in order to minimize possible undesirable chemical degradation of sample and/or changes in physico-chemical properties of soil and sediment by the selected sterilization methods.

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A Clinical Study on the Incidental Pregnancies following Tubal Sterilization Surgery (난관불임술후 발생된 임신에 관한 임상적 고찰)

  • Suh, Byung-Hee;Lee, Jae-Hyun
    • Clinical and Experimental Reproductive Medicine
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    • v.10 no.2
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    • pp.13-23
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    • 1983
  • In recent days, family planning is not only a problem limitted to our country but an important problem for the while world to solve. Up to present, various methods of sterilization have been developed for population control. When a patient with a previous tubal sterilization by operative method develops any symptoms and signs of pregnancy, we strongly consider it ectopic pregnancy and intrauterine pregnancy. As the cause of sterilization failure, we think that the tubal loop sloughs away and tubal ends either unite and recanalization results or heal and failure of union results in fistula. This present study considered of the 34 cases of ectopic pregnancies and 2 cases of intrauterine pregnancies after tubal sterilization by laparoscopy and Pomeroy's method, at the Dept. of Gynecol. in Kyung Hee University Hospital, during 6 years from Jan. 1977 to Dec. 1982. Authors take result in this study retrospectively. 1. Mean age was 33.7 years for the laparoscopic tubal sterilization group, 31.5 years for the Pomeroy tubal sterilization group. 2. The number of mean gravida and parity at the sterilization was 4.9, 2.8 for the laparoscopic tubal sterilization group, 4.2, 2.2 for the Pomeroy tubal sterilization group, :respectively. 3. The number of mean artificial abortion at the sterilization was all 2.2 for the laparoscopic and Pomeroy tubal sterilization groups. 4. Mean intervals from the tubal surgery to the incidental pregnancy was 3.2 years for the laparoscopic tubal sterilization group, and 3.8 years for the Pomeroy tubal sterilization group. 5. 63.3% of the laparoscopic tubal sterilization group had problem of inadequate tubal ligation, in comparison to having no inadequate problem in the Pomeroy tubal sterilization group. 6. The previous tubal sterilized scar was found to be 6 cases (17.6%) of inner portion, 15 cases (44.4%) of midportion, 13 cases (38.2%) of outer portion at the time of operation. The tubal site of ectopic pregnancy was found to be 23 cases (67.6%) of ampullary portion,S cases ( 4.7%) of isthmic and fimbrial portion, respectively. 7. The causes of table sterilization failure were, in order of frequency, technical error (19 cases), fistula formation (6 cases) and recanalization (5 cases) for the laparoscopic tubal sterilization group and fistula formation (2 cases), technical error (l case), recanalization (l case) fo the Pomeroy tubal sterilization group. 8. As the new applicated contraceptive method in incidental pregnant patient, Authors used 2 gravigard insertion for the two intrauterine pregnancy and 34 Pomeroys' tubal ligation, 2 total abdominal hysterectomy (due to associated pelvic inflammatory disease) for the 36 tubal pregnancy.

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Fungal Sterilization Using Microwave-Induced Argon Plasma at Atmospheric Pressure

  • Park, Jong-Chul;Park, Bong-Joo;Han, Dong-Wook;Lee, Dong-Hee;Lee, In-Seop;Hyun, Soon-O.;Chun, Moon-Sung;Chung, Kie-Hyung;Maki Ahiara;Kosuke Takatori
    • Journal of Microbiology and Biotechnology
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    • v.14 no.1
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    • pp.188-192
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    • 2004
  • The main aim of this study was to investigate the sterilization effects of microwave-induced argon plasma at atmospheric pressure on paper materials contaminated with fungi. Plasma-treated filter papers showed no evidence to an unaided eye of burning or paper discoloration due to the plasma treatment. All fungi were perfectly sterilized in less than 1 sec, regardless of strains. These results indicate that this sterilization method for paper materials is easy to use, requires significantly less time than other traditional methods and different plasma sterilization methods, and is also nontoxic.

Improved Cleaning Method for Dental Instruments

  • Kim, In-Geol;Lee, Yun-Ji
    • Journal of Korean Dental Science
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    • v.3 no.2
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    • pp.26-33
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    • 2010
  • We searched at the "PubMed.gov" and "jendodon.com" sites to conduct a literature review on dental instruments that are reused in clinical settings and on infection control involving pre-disinfection or sterilization cleaning/rinsing. The keyword "dental clean" was used for the Web search. We found the present official definition of instrument cleaning performed prior to disinfection or sterilization rather limiting ("removal of foreign matter (soil, organism, etc.) from the instruments"). Thus, we proposed to expand the definition to include the removal of oils applied to protect the metallic instruments and from corrosion, stains, and rust resulting from the frequent reuse of the instruments. Clinicians are found to clean their dental instruments (a) immediately after treating their patients or (b) following their treatment but not immediately afterward. In the latter case, we recommend presoaking to be added. Ultrasonic sterilization of 5~15 minutes is found to be more effective in terms of eliminating residual matter from the instruments compared to other methods. To check on the cleaning results, we recommend visual inspection, which can be quick and practical in clinical settings. The latest products being developed and marketed on the market address the related problems. Nonetheless, research must be continued on the effects of presoak, cleaning/rinsing, disinfection, and high-temperature or heating-based sterilization on the dental instruments and on dental clinicians' practices in cleaning, disinfection, and sterilization. We advise dental clinicians to select the proper cleaning methods and detergents for their instruments to help eliminate or prevent corrosion, staining, and rusting, to reduce the maintenance costs, and to ensure user-friendly instruments/apparatuses.

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Treatment of Ar/O2 Atmospheric Pressure Plasma for Sterilization (아르곤과 산소 대기압 플라즈마 방전 효과를 이용한 살균처리)

  • Son, Hyang Ho;Lee, Won Gyu
    • Applied Chemistry for Engineering
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    • v.22 no.3
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    • pp.261-265
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    • 2011
  • The sterilization effects of atmospheric pressure plasma with the mixture of argon and oxygen were analyzed. The plasma reactor with the shape of dielectric barrier discharge produced the uniform distribution of glow discharge and generated ozone gas effectively according to the various process parameters. The sterilization for E. coli was affected by power, oxygen ratio in the mixture gas, treatment time and distance between reactor and sample. The concentration of ozone was a major source for the sterilization of E. coli, which was enhanced by the increase of power and oxygen ratio. In this study, the effect of atmospheric pressure plasma treatment for the sterilization was confirmed and its result can deliver the atmospheric pressure plasma treatment as the novel sterilization method instead of conventional methods.

Determinants of Sterilization among Married Couples in Korea (우리나라 부부의 영구피임 결정요인)

  • Kim, Ju-Hee;Lee, Sun-Mi;Suh, Moon-Hee;Kang, Dae-Ryong;Chung, Woo-Jin
    • Journal of Preventive Medicine and Public Health
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    • v.40 no.6
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    • pp.461-466
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    • 2007
  • Objectives : The purpose of this study was to examine the determinants of sterilization in South Korea. Methods : This study was based on the data from the Korea National Fertility Survey carried out in the year 2000 by the Korea Institute of Health and Social Affairs. The subjects of the analysis were 4,604 women and their husbands who were in their first marriage, in the age group of 15-49 years. The data were analyzed by multiple logistic regression analysis. Results : Consistent with the findings of previous studies, the woman#s age and the number of total children increased the likelihood of sterilization. In addition, the year of marriage had a strong positive association with sterilization. Interestingly, the number of surviving sons tended to increase the likelihood of sterilization, whereas the woman#s education level and age at the time of marriage showed a negative association with sterilization. Religion, place of residence, son preference, and the husband#s education level, age and type of occupation were not significant determinants of sterilization. Conclusions : The sex of previous children and lower level of education are distinct determinants of sterilization among women in South Korea. More studies are needed in order to determine the associations between sterilization rate and decreased fertility.

Development of Automatic Reading System for Steam Sterilization Indicator (스팀 멸균지시제 자동판독시스템 개발)

  • Song, Hyeok;Kim, Jeong-Rae;Lee, Woo-Cheol
    • The Journal of the Korea institute of electronic communication sciences
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    • v.17 no.4
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    • pp.709-714
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    • 2022
  • The color change of the success of the steam sterilization indicator used in steam sterilization is currently being read by relying on the human eye. The result of color change on the success of sterilization can only be accurately read and judged by trained personnel. Developed an automatic reading system for steam sterilization indicators for the current human-dependent system, and developed an automatic reading device system for reading methods that were previously relied on with the human eye to develop a system that is more accurate and easier for anyone to read.

Evaluation of Washing Method for Sterilization of Gel Container for Ultrasound Inspection (초음파검사용 젤 용기의 제균을 위한 세척방법 평가)

  • Lee, Hee-Jeong;Lee, Suk-Jun;Seoung, Youl-Hun
    • Journal of radiological science and technology
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    • v.41 no.3
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    • pp.241-247
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    • 2018
  • The purpose of this study was to compare the ultrasound gel container washing methods for the sterilization of contaminants and to find the useful methods for the prevention of infection caused by the ultrasonic gel containers. In this experiment, a 250 mL ultrasonic gel container was used, and the ultrasonic gel used was a non-sterile gel (ECO GEL 99, SeungWon Medical, Korea). In order to evaluate the degree of contamination, new 250 mL 15 containers were divided into 5 groups to perform five types of washing by no treatment, washing with water, washing with soap, washing with bottle cleaner and high disinfection level washing. After the washing process, filled the gel container with gel and after 2 weeks, the number of colonies in the gel container was sampled repeatedly twice in the same ultrasonic laboratory and compared before and after washing. As a result of among the five cleaning methods used in this study, 87.2% and 88.9% of the soapy water washing (p = 0.028) and high level washing (p = 0.027) showed significant bacterial reduction rates, respectively. Our findings conclusively an ultrasonic gel container cleaning method for removing contaminants has been found to be an effective sterilization method at a low cost with a soapy water cleaning method. Therefore, it is expected that it will be helpful to prevent the infection caused by the ultrasonic gel container by suggesting the sterilization cleaning method that is practically useful in this study.

Sterilization Effect by the Nd:YAG Laser pulse (Nd:YAG 레이저 펄스에 따른 멸균효과)

  • Jeong, Kyeonghwan;Jung, Dongkyung;Park, Jeongkyu;Choi, Hyeonwoo;Seo, Jeongmin
    • Journal of the Korean Society of Radiology
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    • v.14 no.5
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    • pp.695-703
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    • 2020
  • The use of medical devices is increasing due to the development of medical technology. Among medical devices, it is often used in the human body for graft and treatment. Therefore, in medical institutions, various sterilization methods according to the type and material of medical devices are applied to prevent infection. Hydrophosphite (HA) materials are the most popular in bone grafts. We would like to present a sterilization method using Q-switch Nd:YAG laser with high output energy among non-ionizing radiation suitable for small medical devices. In this study, sterilization power was most ideally shown at UV wavelengths of 1.5 W, 266 nm, and 10 pulses. Different wavelength bands; infrared and visible light; showed passive sterilization, and ultraviolet A and C showed differences in sterilization according to the pulse. In laser sterilization were differences that found according to the wavelength bands and pulses.

A study of $Smartpeg^{TM}'s$ lifetime according to sterilization for implant stability (임플랜트 안정성을 위한 자기공명막대의 소독방법에 따른 수명에 관한 연구)

  • Won, Ho-Yeon;Cho, In-Ho;Lee, Joon-Seok
    • The Journal of Korean Academy of Prosthodontics
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    • v.46 no.1
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    • pp.42-52
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    • 2008
  • Purpose: Resonance Frequency Analysis(RFA) technique can be used as an effective method in measuring the implant stability and documenting the clinical results. This technique also determines how stable the implant is before performing a prosthetic practice. Having become one the guidelines of the implant therapy whose final objective is the immediate loading, the $Osstell^{TM}$ mentor is giving a lot of information to the clinicians recently. In this communication, experiments were performed to investigate how reliable the measured ISQ values by $Osstell^{TM}$ mentor are, and to see if those are also stable even after sterilization. As five objectives: 1) How stable measured ISQ values after fixation $Smartpeg^{TM}s$ for 400 times. 2) How stable measured ISQ values after 'attach-detach'$Smartpeg^{TM}'s$ for 400 times. 3) How stable measured ISQ values after clinical sterilization methods. 4) How stable measured ISQ values after repeatedly sterilization in autoclave for 10 times. 5) What is the critical temperature which is lost the magnetism of $Smartpeg^{TM}$. Materials and Methods: Clinical sterilization methods(Autoclave sterilization, Dentistar sterilization, Ultra violet sterilization, Vacuum dry unit sterilization, Boiling water sterilization, combined $H_{2}O_{2}$ and Alcohol sterilization).$Smartpeg^{TM}s$. D3 Block bone($3{\times}9{\times}2cm$). Osstem implant(${\emptyset}4.1$-10mm).$Osstell^{TM}$ mentor. Individual experiment was used 8 number of $Smartpeg^{TM}s$ and they had measured to ISQ values of before experiment and after experiment. Results: 1. The measured ISQ values did not change after fixation $Smartpeg^{TM}s$ for 400 times. 2. There was no significant changes in the measured ISQ values of 'attach-detach $Smartpeg^{TM}s'$ for 400 times. 3. The measured ISQ values did not change after the usual clinical sterilization methods. 4. The measured ISQ values did not change after sterilization in autoclave for 10 times. 5. It was impossible to exactly measure the critical temperature which is lost the magnetism of $Smartpeg^{TM}s$. But, the results was resulted to lost its magnetism in higher temperature than $150^{\circ}C$/10 minute. Conclusion: The measured ISQ values showed insignificant differences in case of no changes in the magnetism of the $Smartpeg^{TM}s$. It seems that the $Smartpeg^{TM}s$ can be used repeatedly in every measurement if the original magnetisms of the $Smartpeg^{TM}s$ can be recognized. There seems to be no significant changes in the measured ISQ values of 'attach-detach $Smartpeg^{TM}s'$ only if the screw pitches were unimpaired. The clinical sterilization methods seems acceptable because the result was resulted to lost its magnetism in higher temperature than $150^{\circ}C$/10minute.