• Proceedings of the Zoological Society Korea Conference
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• 1999.04a
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• pp.57.2-57
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• 1999

No Abstract, See Full Text

• Clinical and Experimental Reproductive Medicine
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• v.10 no.2
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• pp.39-47
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• 1983

The XI World Congress on fertility and sterility was held during 26th June-2nd July 1983, in Dublin, Ireland. The general view and impression of the congress are as follows. Scientific Programme 1. Main sessions were consisted of a keynote speaker 30 minutes with 4 supporting speakers for 20 minutes each, followed by discussion between the panelist and audience. 2. Related communication sessions were consisted of a series of 10 minutes papers and 5 minutes discussion. 3. Workshop sessions had a free wheeling time-table of audience/expert interaction, subjects concerned day to day practicalities of fertility and sterility. 4. Special symposium sessions had contributed from a number of invited experts in a particular field under discussion. 5. National society symposium sessions were organised and ran by fertility societies affiliated to IFFS. 6. Meet-the-authnr poster sessions, Film and Video sessions, Medical and industrial exhibitions are also introduced here. Business Programme 1. The meeting of executive and scientific commitees IFFS were held on June 26th, July 1st, and general assembly on June 28th, and July 1st. 2. Accreditation : IFFS Dublin 83, is recognised for 32 cognates formal learning by the American College of Obstetricians and Gynecologists. Social Programme 1. Dublin city orientation tour, welcome reception, Opening and closing ceremonies, evening of traditional Irish entertainment, Irish night at Powercourt Town-house Center, the banquet, State reception-(hosted by Minister of Health and Social secretary at Dublin Castle, and by the Lord Mayor of Dublin at National Concert Hall)-are introduced briefly in this paper. 2. The congress tour;, such as Georgian Dublin and shopping tour, Garden of Ireland tour, Boyne Valley tour and Curragh of Kildare tour were prepared during congress. 3. After closing the congress, for some of delegates an opportunity to sample, A taste of Dublin, such as Abbey theater, Abbey tavern and Jury's Cabaret etc were available.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.35 no.6
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• pp.481-486
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• 1990

A survey was carried out on the cold-induced sterility of paddy rice in 1988 in the alpine area of Kangweon province when cold spell occurred during late July to early August, During this period minimum temperature as low as 8.5$^{\circ}C$ and 5.7$^{\circ}C$ was recorded at Dunnae and Jinbu, respectively. The rice cultivars, which encountered this cold spe]J at meiotic stage of microspore, were damaged by sterility in most alpine areas of higher than 300m in altitude. To secure spilkelet fertility higher than 80% it was estimated that the minimum and average air temperature accumulated during 13 days of meiotic stage should be higher than 2$25^{\circ}C$ and 285$^{\circ}C$, respectively and/or the duration of lower than 17$^{\circ}C$ in minimum temperature should not exceed fivedays during that period.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.13 no.4
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• pp.405-411
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• 1991

To determine the sterility of the prepared allogeneic bone of the human, culture of the allografts prior to implantation was performed on fresh-frozen and freeze-dried bone. Before the use of allografts to the patients, it must be confirmed about the sterility, cellular cytotoxicity, immune reaction, and osteoinductive potential as a biomaterials. Oral and maxillofacial surgeons demand for allograft bone will be increased in the future. Wonkwang Bone Bank attempted to meet this demand, has performed series of experimental study on the allograft bone of the Koreans to evaluate the physical and chemical suitability of the bone since the surgeons applications will have broadened from benign cystic lesions to fracture malunions and non-unions, large segmental defects, and whole-bone allorgrafts after tumor surgery. The results obtained were as follows: 1. Freeze-drying(FD) only shoed some bactericidal effects of the normal and osteo bone but in cases of performing EO gas sterilization, the FD effects was not clear. 2. The fact that FD has little effect than the EO gas sterilization on normal bone postulated that the presence of microbiota may be due to an operation and bone processing procedure. 3. FD and EO gas sterilization had a remarkable effect on the osteo bone. 4. The sterilization effect were EO gas, Freeze-drying, Fresh-Frozen with descending order. But all sterilization method were not complete to preserve and implant allograft bone. We are now performing further continuous study on the radiation and chemical sterilization procedure to make safe and complete allograft bone.

• The Korean Journal of Nuclear Medicine Technology
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• v.12 no.1
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• pp.74-77
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• 2008

The radiopharmaceuticals are routinely injected to blood vessel for acquiring PET image. For this reason, It is imperative that they undergo strict quality control measures. Especially, Sterility test is more important than any other quality control procedures. According to the FDA guideline, It requires filter integrity test used in the processing of sterile solutions. Among several methods, we can decide to use bubble point test. We usually use vented GS-filters (Millipore co., USA) which are sterilizinggrade (0.22 um pore size) and are placed upper site on product vial. After the synthesis of $^{18}F$-FDG, solutions wet the membrane in filter and then go into the product vial. By all synthesis steps have finished, we can observe the presence of the bubbles in the product vial. Since we have started this study, we have never found any bubbles in the product vial. Because the maximum pressure intensity of the filter which has set by manufacturer is up to 5 bars, but helium gas pressure is up to 1 bar in our module system. So, we can make 5 bars pressure using helium gas bombe and increase pressure up to 5 bars step by step. However, it does not happen to anything in vial.

• Plant Biotechnology Reports
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• v.3 no.4
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• pp.309-315
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• 2009

Molecular markers developed from the flanking sequences of two cytoplasmic male sterility (CMS)-associated genes, orf456 and ${\Psi}atp6-2$, have been used for marker-assisted selection of CMS in pepper. However, in practice, the presence of orf456 and ${\Psi}atp6-2$ at substoichiometric levels even in maintainer lines hampers reliable selection of plants containing the CMS gene. In this study, we developed a novel CMS-specific molecular marker, accD-U, for reliable determination of CMS lines in pepper, and used the newly and previously developed markers to determine the cytoplasm types of pepper breeding lines and germplasms. This marker was developed from a deletion in a chloroplast-derived sequence in the mitochondrial genome of a CMS pepper line. CMS pepper lines could be unambiguously determined by presence or absence of the accD-U marker band. Application of orf456, ${\Psi}atp6-2$and accD-U to various pepper breeding lines and germplasms revealed that accD-U is the most reliable CMS selection marker. A wide distribution of orf456, but not ${\Psi}atp6-2$, in germplasms suggests that the pepper cytoplasm containing both orf456 and ${\Psi}atp6-2$ has been selected as CMS cytoplasm from cytoplasm containing only orf456. Furthermore, factors other than orf456 may be required for the regulation of male sterility in pepper.

• Korean Journal of Plant Resources
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• v.11 no.1
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• pp.80-85
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• 1998

The influence of elevated CO2 and temperature on growth parameters, biomass production and its partitioning of rice (Oryza sativa L.cv. Chukwangbyeo) were investigated in the three experiments (1991-1993). Rice plants were grown from transplanting to harvest at either ambient(350ppm) or elevated CO2 concentrations (690 or 650ppm) in combination with either four or seven temperature regimes ranging form ambient temperature (AT) to AT plus 3$^{\circ}C$.From transplanting to panicle initiation, crop growth rate (CGR) was enhanced by up to 27% with elevated CO2 , primarily due to an an increase in leaf area index. although net assimilatiion rate was also greater at elevated CO2. The effect of elevated CO2 varied with temperature. During the reproductive phase, CGR declined linearly with increased temperature, and was greater at elevated CO2 . Elevated CO2 increased final crop biomass and panicle weight 30% respectively at AT(27.6$^{\circ}C$ : 1991) . However, there was no significant effect of elevated CO2 on panicle weight at AT plus 3$^{\circ}C$, where severe spikelet sterility occurred. There was no significant effect of elevated CO2 on panicle weight at AT plus 3$^{\circ}C$, where severe spikelet sterility occurred. There was also no effect of CO2 on biomass pratitioning into vegetative and reproductive organs (harvest index)) at AT, although higher temperature could affect that by inducing spikelet sterility. These results suggest that elevated CO2 could enhance rice producivity througth promoted growth and biomass production , but its positive effects may be less at higher temperatures.

• Clinics in Orthopedic Surgery
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• v.10 no.4
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• pp.427-432
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• 2018

Background: The purpose of this study was to evaluate the usefulness of sonication technique for microbiological diagnosis and the sterility of the recycled autoclaved femoral components from infected total knee arthroplasty (TKA) using a sonication method. Methods: Nineteen femoral implants explanted from patients with infected TKA were sterilized with a standard autoclave method. Standard culture of the fluid before and after sonication of the sterilized implants was performed to detect pathogenic microorganisms. Additional experiments were performed to evaluate the sterility of the recycled implant by inducing artificial biofilm formation. Methicillin-resistant Staphylococcus aureus (MRSA) was inoculated into 10 implants and sterilization in a standard autoclave was performed, and then the fluid was cultured before and after sonication. Results: Two of the 19 sterilized implants were positive for growth of bacteria after sonication, whereas no growth was detected in the cultured fluid from the sterilized implants before sonication. The bacteria were Staphylococcus species in all two cases. In one of 10 implants inoculated with MRSA, the culture was positive for growth of bacteria both before and after sonication. However, Staphylococcus epidermidis was cultured from both occasions and thus this implant was thought to be contaminated. Conclusions: We found sonication for identification of pathogens could be helpful, but this finding should be interpreted carefully because of the possibility of contamination. Sterilization of an infected femoral implant with an autoclave method could be a good method for using the temporary articulating antibiotic spacer in two-stage revision arthroplasty.

• Journal of Ginseng Research
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• v.43 no.2
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• pp.261-271
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• 2019

Background: Protopanaxatriol (PPT) is an aglycone of ginsenosides, which has high medicinal values. Production of PPT from natural ginseng plants requires artificial deglycosylation procedures of ginsenosides via enzymatic or physicochemical treatments. Metabolic engineering could be an efficient technology for production of ginsenoside sapogenin. For PPT biosynthesis in Panax ginseng, damarenediol-II synthase (PgDDS) and two cytochrome P450 enzymes (CYP716A47 and CYP716A53v2) are essentially required. Methods: Transgenic tobacco co-overexpressing P. ginseng PgDDS, CYP716A47, and CYP716A53v2 was constructed via Agrobacterium-mediated transformation. Results: Expression of the three introduced genes in transgenic tobacco lines was confirmed by Reverse transcription-polymerase chain reaction (RT-PCR). Analysis of liquid chromatography showed three new peaks, dammarenediol-II (DD), protopanaxadiol (PPD), and PPT, in leaves of transgenic tobacco. Transgenic tobacco (line 6) contained $2.8{\mu}g/g$ dry weight (DW), $7.3{\mu}g/g$ DW, and $11.6{\mu}g/g$ DW of PPT, PPD, and DD in leaves, respectively. Production of PPT was achieved via cell suspension culture and was highly affected by auxin treatment. The content of PPT in cell suspension was increased 37.25-fold compared with that of leaves of the transgenic tobacco. Transgenic tobacco was not able to set seeds because of microspore degeneration in anthers. Transmission electron microscopy analysis revealed that cells of phloem tissue situated in the center of the anther showed an abnormally condensed nuclei and degenerated mitochondria. Conclusion: We successfully achieved the production of PPT in transgenic tobacco. The possible factors deriving male sterility in transgenic tobacco are discussed.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.4
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• pp.359-367
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• 1985

This experiment was conducted to study effect of cold water damage on some growth characters related to source and sink at reproductive growth stage in Jinan (sea level 303m). The cold water irrigation duration had irrigated 4, 8 and 12 days at panicle formation stage and reproduction division stage compared to perennial water irrigation. Cold water irrigation shortened culm length and panicle length and degree of panicle exsertion. The shortening effect appeared great at lower internodes when treated at panicle formation stage but at higher internodes when treated at reduction division stage. Cold water irrigation decreased the number of secondary branches and spikelets per panicle, and increased the number of degenerated spikelets being high degeneration when treated at panicle formation stage. Spikelet sterility and impediment of grain filling were affected by duration of cold water irrigation being great when treated at spikelet primodium differentiation stage and reduction division stage in particular. Grain weight was also reduced. Significant relationship existed between spikelets sterility, grain filling and yield. The degeneration of secondary branches and spikelets correlated with leaf area but spikelet sterility and yield with culm length, panicle length and panicle exsertion.