• Title/Summary/Keyword: Sterility

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Identification of Heterosis QTLs for Yield and Yield-Related Traits in Indica-Japonica Recombinant Inbred Lines of Rice (Oryza sativa L.)

  • Kim, Chang-Kug;Chu, Sang-Ho;Park, Han Yong;Seo, Jeonghwan;Kim, Backki;Lee, Gileung;Koh, Hee-Jong;Chin, Joong Hyoun
    • Plant Breeding and Biotechnology
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    • v.5 no.4
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    • pp.371-389
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    • 2017
  • Supplying sufficient rice to growing populations is a global challenge. Hybrid indica rice varieties exploiting heterosis have increased yields, but inter-subspecific crosses between indica and japonica varieties are hampered by sterility. Examination and genetic understanding of yield heterosis in indica/japonica crosses addressing yield barriers are basic requirements. In this study, QTLs for heterosis of yield traits were identified in indica-japonica recombinant inbred lines (RILs) using a total of 178 RILs originating from Dasanbyeo (indica) ${\times}$ TR22183 (japonica) (DT-RILs) and their backcrossed populations. Nine of sixty-six major quantitative trait loci (QTLs) identified in DT-RILs exhibited heterosis. Heterosis QTLs clustered with other traits on chromosomes 1, 4, and 8, and clusters were conserved between different RILs. The clusters contained several known yield enhancement genes/QTLs. Specific heterotic allele combinations contributed to four major heterosis QTLs, particularly for panicle and spikelet number traits. Heterosis for yield and yield-related traits was explained by the harmonized effects of overdominance, dominance, and epistatic interactions in inter-subspecific breeding populations.

Developmental inhibition of Drosophila suzukii by ionizing radiation

  • KIM, Junheon;KIM, Jeongmin;LEE, Yeon Jeong;PARK, Chung Gyoo
    • Entomological Research
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    • v.48 no.5
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    • pp.331-338
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    • 2018
  • Spotted wing drosophila (SWD) has emerged as a major invasive insect pest of small berry fruits in the Americas and Europe since the late 2000s. Thus, phytosanitary treatment of commodities for export is imperative to prevent the movement of viable SWD to newer areas. In the present study, all developmental stages of SWD were irradiated with different doses of gamma and electron beam radiation to assess developmental inhibition to identify potential quarantine doses of the radiations. Ionizing radiation induced developmental inhibition of all stages of SWD. The effective doses for 99% inhibition ($ED_{99}$) of hatching, pupariation, and adult emergence from irradiated eggs for gamma radiation were 882, 395 and 39 Gy, respectively, compared with 2849, 687, and 41 Gy, respectively, for electron beam radiation. The $ED_{99}$ for inhibition of pupariation and adult emergence in irradiated larvae were 703 and 47 Gy, respectively, for gamma radiation, and 619 and 33 Gy, respectively, for electron beam radiation. Pupal irradiation did not completely inhibit adult emergence, even at 300 Gy. However, irradiation with ${\geq}100Gy$ of puparia induced adult sterility, with no egg production at all. The $ED_{99}$ for inhibition of $F_1$ egg hatchability from adults irradiated with gamma radiation and electron beam radiation was estimated to be 424 and 125 Gy, respectively. The results of the present study suggest that gamma radiation and electron beam radiation are alternatives for phytosanitary treatment. Irradiation with 100 Gy could be suggested as a potential dose for egg, larval, and pupal quarantine treatment of SWD.

Survival and Goodness in a Post - apocalyptic Future: Cormac McCarthy's The Road (포스트 - 묵시록 미래의 생존과 선의 실행: 코맥 매카시의 『로드』)

  • Sung, Junghye
    • English & American cultural studies
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    • v.16 no.2
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    • pp.71-88
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    • 2016
  • Cormac McCarthy's The Road depicts a post-apocalyptic future in which the time and reason of catastrophe are not specified, but it can be seen to project the contemporary social and ethical concerns in the dismal setting. In this respect, the journey the man and boy depart for to the South is on one hand a journey to seek a warmer place to support their lives in the literal sense and, on the other hand, a journey to seek 'what a human being is' in the devastated world in a metaphorical sense since they face extremely harsh and tremendously poignant conditions in which their creed as human beings is tested. This paper aims to explore the hazards of the current society that the text criticizes and the morality and ethical values to be preserved and pursued. The second chapter examines how the text describes the contemporary crisis through the dark and coldness of the land and its sterility. The land produces almost nothing as the entire surface was scorched and is now covered with thick ashes. It shows perfectly a destroyed and irrecoverably frozen land. Throughout this desolate and ruined land, the atrocity and violence of the survivors goes beyond the limit. Ravaging strangers and plundering villages are widely spread. These conditions mirror the apparent selfishness and immorality of the recent society. The third chapter analyzes the man's inconsistent or dualistic narrative on the good. He knows what the good is but doesn't allow the boy to demonstrate the good behavior on others. His conflict is rooted in his hope to protect his son from being attacked by others. Therefore it can be interpreted that the meaning and orders of living in this post-apocalyptic period are uncertain and indecisive. The fourth chapter examines the belief the man and the boy clings to. Unlike the man's contradictory decisions, he shows definite firmness to be 'the good' by not eating people and carrying 'the fire.' Until he dies, the man endeavors to protect his son and have him acquire the moral conviction and strength to carry the fire in the world. In conclusion, the text reads the current society critically and highlights the importance of the humanity that must not be discarded throughout the generations.

"Gerontion" and The Waste Land: Why Did Eliot Intend to Make "Gerontion" a Preface to The Waste Land? (『황무지』와 「게론티온」-왜 엘리엇이 「게론티온」을 『황무지』 서시로 사용하려 했었나?)

  • Lee, Cheol hee
    • Journal of English Language & Literature
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    • v.55 no.2
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    • pp.359-382
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    • 2009
  • Eliot's The Waste Land represents the last century in many respects. While working on the poem in cooperation with Pound, Eliot intended to make "Gerontion" a prelude in The Waste Land. But, as we read in his letter to Eliot, Pound advised him against it. As a result, Eliot had excluded it from The Waste Land. "Gerontion" was published separately, as an independent poem. Between "Gerontion" and The Waste Land, we find that the theme and the techniques are very much alike. However, for this very reason Eliot and Pound must have had thought differently. Eliot must have thought that "Gerontion" would serve well as a preface to the long poem, The Waste Land. It will provide a good introduction to the long poem, he may have thought. In the meantime, Pound must have thought that such similarities in theme and techniques would weaken both works, which would be redundant. To Pound, it would be too much to have the summary of everything that is to be repeated in The Waste Land. Eliot intuitively followed Pound's judgment. Both "Gerontion" and The Waste Land have similarities in theme and techniques. The theme of both works is "aimlessness, spiritual sterility, barrenness" in modern man living in the waste land. For example, in "Gerontion," there appear an old man Gerontion, Mr. Silvero, Hakagawa, Madame de Tornquist, Fraulein von Kulp, who are representative of spiritual barrenness of modern world; in the same context, in The Waste Land those who are most representative of modern world are the Typist, clerk, Thames's daughters, Madamn Sosostris, Tiresias, Phelabas. And in terms of techniques, "Gerontion" and The Waste Land both use dramatic monologues, allusions, and the techniques of modern art, such as montage and mosaic. Here in these works Eliot in fact practises his theory of the "Objective Correlative" that he has invented.

Comparison of irradiated and non-irradiated acellular dermal matrices in breast reconstruction under radiotherapy

  • Woo, Soo Jin;Ha, Jeong Hyun;Jin, Ung Sik
    • Archives of Plastic Surgery
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    • v.48 no.1
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    • pp.33-43
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    • 2021
  • Background Acellular dermal matrices (ADMs) have become an essential material for implant-based breast reconstruction. No previous studies have evaluated the effects of sterility of ADM under conditions of radiation. This study compared sterile (irradiated) and aseptic (non-irradiated) ADMs to determine which would better endure radiotherapy. Methods Eighteen male Balb/C mice were assigned to the control group with no irradiation (group 1) or one of two other groups with a radiation intensity of 10 Gy (group 2) or 20 Gy (group 3). Both sterile and aseptic ADMs were inserted into the back of each mouse. The residual volume of the ADM (measured using three-dimensional photography), cell incorporation, α-smooth muscle actin expression, and connective tissue growth factor expression were evaluated. The thickness and CD3 expression of the skin were measured 4 and 8 weeks after radiation. Results In groups 2 and 3, irradiated ADMs had a significantly larger residual volume than the non-irradiated ADMs after 8 weeks (P<0.05). No significant differences were found in cell incorporation and the amount of fibrosis between irradiated and non-irradiated ADMs. The skin was significantly thicker in the non-irradiated ADMs than in the irradiated ADMs in group 3 (P<0.05). CD3 staining showed significantly fewer inflammatory cells in the skin of irradiated ADMs than in non-irradiated ADMs in all three groups after 4 and 8 weeks (P<0.05). Conclusions Under radiation exposure, irradiated ADMs were more durable, with less volume decrease and less deposition of collagen fibers and inflammatory reactions in the skin than in non-irradiated ADMs.

Influence of Nitrogen Application and Shading on the Sterile-type Cold Injury in Rice (질소시비와 차광이 벼 장해형냉해에 미치는 영향)

  • Kim, Hee-Dong;Tetsuo, Satake;Kim, Young-Ho;Kim, Byeong-Hyeon;Ree, Dong-Woo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.3
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    • pp.252-260
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    • 1989
  • This experiment was carried out to clarify the causes of sterility in terms of pollination characteristics for the sterile-type cold damage as influenced by amount of nitrogen application and shading conditions in rice plants. The results obtained are as follows: The number of young microspore per anther was not changed greatly by amount of nitrogen application. The number of ripened pollen grains per anther decreased according to increase in nitrogen application and shading degree, but the anther length and stigma length were not significantly affected by those factors. The number of pollen grains on stigma decreased by intense shading. The fertility decreased with increased nitrogen application and intensified shading. The elongation of auricle distance per day was less in less nitrogen application and intense shading. The internode length of the first and the second from the top were shortened with intense shading, but that of the fourth was elongated. The number of spikelet per panicle decreased with increase in shading intensity.

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Effect of Sodium Methyl Arsenate as a Male Gametocide on Rice (Oryza sativa L.) (수도 웅성불임제로서 Sodium Methyl Arsenate의 효과)

  • Cho, Soo-Yeon;Son, Young-Hee;Choi, Hae-Chun;Moon, Huhn-Pal;Park, Rae-Kyeong;Park, Nam-Kyu;Kim, Chong-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.1
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    • pp.55-59
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    • 1989
  • Sodium methyl arsenate has been evaluated as a male sterilizing agent for the system of producing hybrid rice seeds. The compound was the most effective at the concentration of 0.02%. When applied as a foliar spray to four rice varieties at 15 days before heading, sodium methyl arsenate has produced 99% male sterility. But the most effective time for application of the compound was 5 days before heading because of its phytotoxic effects. Effective application volume of the compound solution has depended on the growth of the plants treated. Varietal difference on the activity of the compound has been detected.

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Effects of Elevated Air Temperature on Yield and Yield Components of Rice (온도 상승 조건이 벼의 수량 및 수량구성요소에 미치는 영향)

  • Lee, Kyu-Jong;Nguyen, Duc-Nhuan;Choi, Doug-Hwan;Ban, Ho-Young;Lee, Byun-Woo
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.17 no.2
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    • pp.156-164
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    • 2015
  • High temperature stress would affect rice production in the future as heat wave is expected to occur frequently under climate change conditions. The objective of this study was to obtain rudimentary information to assess the impact of heat stress on rice yield and its yield component in Korea. Two rice cultivars "Hwaseongbyeo" (Japonica) and "Dasanbyeo" (Tongil-type) were grown at different nitrogen fertilization levels in two seasons. These cultivars were grown in 1/5000a Wagner pot placed within four plastic houses where temperature was controlled at ambient, ambient$+1.5^{\circ}C$, ambient$+3^{\circ}C$ and ambient$+5^{\circ}C$ throughout the rice growing season in Suwon ($37^{\circ}16^{\prime}N$, $128^{\circ}59^{\prime}E$), Korea. The degree of temperature change affected grain yield whereas the level of nitrogen had little impact on grain yield. The number of panicle per pot and spikelet per panicle were not significantly different among temperature treatments in both cultivars tested. In contrast, 1000-grain weight and ripened grain ratio were decreased significantly under the treatments raising the air temperature to the level of $5.0^{\circ}C$ and $1.5^{\circ}C$ above the ambient air temperature in Dasanbyeo and Hwaseongbyeo, respectively. Reduction of 1000-grain weight and ripened grain ratio under the temperature treatments of $3.0^{\circ}C$ and $5.0^{\circ}C$ above the ambient air temperature resulted in significantly less grain yield for Dasanbyeo and Hwaseongbyeo, respectively. The greater sensitivity of grain yield to temperature increase in Dasanbyeo was attributable to the sharp decrease of 1000-grain weight and ripened grain ratio with the temperature rise above $23^{\circ}C$ during ripening period. On the other hand, Hwaseongbyeo had little variation of them in the temperature range of $23-27^{\circ}C$. These results suggested that grain yield would decrease under future climate conditions due to grain weight decreased by shorter grain filling period as well as the ripened grain ratio reduced by spikelet sterility and early abortion of rice kernel development. Thus, it would be essential to use cultivars tolerant to heat stress for climate change adaptation, which merits further studies for developing varieties that have traits to avoid spikelet sterility and early abortion of rice kernel, e.g., early morning flowering, under heat wave.

Studies on the mechanism in the induced to unfertilized eggs(male sterility of Silkworm) by protected environment during pupae period (용기의 보호환경에 따른 불수정란(웅성불임잠)의 유발기구에 관한 조사연구)

  • 윤종관;오준식
    • Journal of Sericultural and Entomological Science
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    • v.15 no.2
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    • pp.9-14
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    • 1973
  • In order to find out effects of the generative power of silk worm moth which have been brought up in the high temperature accommodation at their pupa stage. For this specific study, 9 different kinds of male silk worms have been selected as specimen. All those specimen were brought up in the normal temperature at their larvae stage and after the pupation period they have been accommodated in the condition of high temperature for a certain length in accordance with the study programme. Afterwards, those mlae specimen were copulated with Suwon jam 103${\times}$104 which were all brought up in normal conditions. This study was carried out to find the copulation function as well as the ratio of unfertilized eggs(male sterility test). Results of study have been revealed as follows: 1) Although some differences were observed, male pura which have been brought up in the condition of high temperature shown the low rate of unfertilized eggs rather than those were brought up in the normal conpition. 2) In this group the eclosion(emergency) has been found to be poor rather, than those specimen brought up in normal conditions. 3) The copulation function of Moran, Daedong, J124 and C108 specimen were found to be poor than those of Suwon jam. 4) Fertility rate of Moran, Daedong, J124 and C108 was found to be around 65%. This figure is rather lower than what we normally expect. 5) Unfertilized egg ratio of Moran, Daedong and C108 were found to be around 20% if they were brought up in the condition of high temperature for one day from the time of pupation: 40% at 2 days, and 70% at 3 days duration. More than 3 days treatment has shown no progress in the unfertilized egg ratio. 6) One day's treatment for the pupa at the later stage has shown the unfertilized egg ratio of about 10%; 20% at 2 day's treatment, 35% at 3 day's treatment, 40-60% at 4 day's treatment, more than 60% at 5 day's treatment, and the 70% of fertilized egg ratio was only observed when the treatment days come to 7 days. It was understood that the unfertilized egg ratio was high at the antepupa stage rather than that of post-pupa stage. 7) According to the result of observation the sperm in copulatory pouch and seminal receptacle out of the normal female silk worm which have been copulated with the male brought up in the condition of high temperature at their moth stage. The reproduction system found in the control zone has been found to be normal and the sperm is amountful and active in motion while the sperm found in the treatment to be limited in amount and slow in motion. The observation was made within 5 hours from the copulation. 8) According to the result of observation of sperm of seminal receptacles of the female silkworm moth, and according to that observation of sperm in the seminal receptacle in female silkworm moth, the amount of sperm and mobility in the female moth brought up in high temperature were poor comparing that were brought up in normal temperature zone. Some of them are even found to be no trace of such. 9) Appearance and mosle of the copulatory organ of the male silkworm moth was found to be no anatomical change. 10) Testis of the later pupa stage which was brought up in the high temperature was found to be almost net developed to anucleate sperm and they are degenerated at stage of between maturation division and sperm abnormal stage. Mean time at control zone, the formation of anucleate sperm was already observed.

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Inheritance Study of Male Sterile Transformants Containing Pollen-specific Promoter and Diphtheria Toxin A Gene (수술특이프로모터와 디프테리아 독성 유전자에 의한 웅성불임 형질전환체의 후대 유전분석)

  • Park, Young-Doo;Kim, Hyun-Uk;Park, Beom-Seok;Jin, Yong-Moon
    • Horticultural Science & Technology
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    • v.18 no.3
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    • pp.342-347
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    • 2000
  • The objectives of this study were to investigate the genetic and phenotypic features of male sterile transformants by pollen-specific expression of diphtheria toxin gene and to find out inheritance patterns of transgene to the next generation. When backcrossed (BC) progenies were tested for expression of kanamycin resistance ($Km^R$), 9 lines out of 13 lines, except 4 lines ($BC_{1}5-13,\;BC_{1}5-23,\;BC_{1}5-28,\;BC_{1}5-32$), showed the ratio of $Km^R$ to kanamycin sensitive ($Km^S$), from 1:30 to all $Km^S$. As a result, they were much lower than Mendelian segregation of a dominant gene. To determine whether male sterility is a heritable and stable trait, 5 male sterile plants ($BC_{1}5-13,\;BC_{1}5-14,\;BC_{1}5-23,\;BC_{1}5-32,\;BC_{1}5-33$ lines) which had different transgene copy numbers were backcrossed as female parents with pollens from wild type. To confirm the existence of the DTx-A gene in the genome of the progenies, PCR was conducted using specific primers of the DTx-A coding region. A PCR band of 428 bp was obtained from each generation, which is the predicted size of the DTx-A gene fragment. Trangenes were inherited to the next $BC_4T_0$ progenies and showed male sterility, however, based on the copy numbers of DTx-A gene male sterile plants did not show predicted ratio. When male sterile plants were backcrossed with fertile plants, fruit capsule sizes and seed settings were relatively reduced from those of selfing wild type plants. The fruit sizes and seed settings were reduced in proportion to the increase in the copy number of DTx-A gene.

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