• 한국동물학회:학술대회논문집
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• 한국동물학회 1999년도 공동 춘계학술대회
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• pp.57.2-57
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• 1999

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• Clinical and Experimental Reproductive Medicine
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• 제10권2호
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• pp.39-47
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• 1983

The XI World Congress on fertility and sterility was held during 26th June-2nd July 1983, in Dublin, Ireland. The general view and impression of the congress are as follows. Scientific Programme 1. Main sessions were consisted of a keynote speaker 30 minutes with 4 supporting speakers for 20 minutes each, followed by discussion between the panelist and audience. 2. Related communication sessions were consisted of a series of 10 minutes papers and 5 minutes discussion. 3. Workshop sessions had a free wheeling time-table of audience/expert interaction, subjects concerned day to day practicalities of fertility and sterility. 4. Special symposium sessions had contributed from a number of invited experts in a particular field under discussion. 5. National society symposium sessions were organised and ran by fertility societies affiliated to IFFS. 6. Meet-the-authnr poster sessions, Film and Video sessions, Medical and industrial exhibitions are also introduced here. Business Programme 1. The meeting of executive and scientific commitees IFFS were held on June 26th, July 1st, and general assembly on June 28th, and July 1st. 2. Accreditation : IFFS Dublin 83, is recognised for 32 cognates formal learning by the American College of Obstetricians and Gynecologists. Social Programme 1. Dublin city orientation tour, welcome reception, Opening and closing ceremonies, evening of traditional Irish entertainment, Irish night at Powercourt Town-house Center, the banquet, State reception-(hosted by Minister of Health and Social secretary at Dublin Castle, and by the Lord Mayor of Dublin at National Concert Hall)-are introduced briefly in this paper. 2. The congress tour;, such as Georgian Dublin and shopping tour, Garden of Ireland tour, Boyne Valley tour and Curragh of Kildare tour were prepared during congress. 3. After closing the congress, for some of delegates an opportunity to sample, A taste of Dublin, such as Abbey theater, Abbey tavern and Jury's Cabaret etc were available.

• 한국작물학회지
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• 제35권6호
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• pp.481-486
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• 1990

1988년도 강원도내 산간고랭지 등 국지적으로 발생한 랭해피해양상을 조사분석한 결과를 요약하면 다음과 같다. 1. 저온 경과시기는 지역간 차이는 있으나 7월 23일부터 8월 2일 사이로 감수분열기에 해당된 벼가 장해형 랭해를 유발하였다. 이때 최저극기온 횡성군 둔내면은 8.5$^{\circ}C$, 평창군 진부면은 5.7$^{\circ}C$였다. 2. 저온피해지역은 표고 300m 이상 산간고랭지가 대부분 해당되는 지역이었다. 3. 랭해피해지역의 임실비율이 80% 이상 되려면 감수분열기 13일간 평균기온 적산온도 285$^{\circ}C$, 최저기온 적산온도 2$25^{\circ}C$ 이상, 17$^{\circ}C$이하 지속일수 5일 이하로 경과하여야 함을 추정할 수 있었다. 4. 감수분열기 저온피해정도별 수량은 총재배면적 $\times$ 총피해품종면적/총면적 $\times$ 감수 분열제 피해면적/총피해품종면적 $\times$ 총예상수량 $\times$ 임실장해율로 산출할 수 있다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제13권4호
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• pp.405-411
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• 1991

To determine the sterility of the prepared allogeneic bone of the human, culture of the allografts prior to implantation was performed on fresh-frozen and freeze-dried bone. Before the use of allografts to the patients, it must be confirmed about the sterility, cellular cytotoxicity, immune reaction, and osteoinductive potential as a biomaterials. Oral and maxillofacial surgeons demand for allograft bone will be increased in the future. Wonkwang Bone Bank attempted to meet this demand, has performed series of experimental study on the allograft bone of the Koreans to evaluate the physical and chemical suitability of the bone since the surgeons applications will have broadened from benign cystic lesions to fracture malunions and non-unions, large segmental defects, and whole-bone allorgrafts after tumor surgery. The results obtained were as follows: 1. Freeze-drying(FD) only shoed some bactericidal effects of the normal and osteo bone but in cases of performing EO gas sterilization, the FD effects was not clear. 2. The fact that FD has little effect than the EO gas sterilization on normal bone postulated that the presence of microbiota may be due to an operation and bone processing procedure. 3. FD and EO gas sterilization had a remarkable effect on the osteo bone. 4. The sterilization effect were EO gas, Freeze-drying, Fresh-Frozen with descending order. But all sterilization method were not complete to preserve and implant allograft bone. We are now performing further continuous study on the radiation and chemical sterilization procedure to make safe and complete allograft bone.

• 핵의학기술
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• 제12권1호
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• pp.74-77
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• 2008

The radiopharmaceuticals are routinely injected to blood vessel for acquiring PET image. For this reason, It is imperative that they undergo strict quality control measures. Especially, Sterility test is more important than any other quality control procedures. According to the FDA guideline, It requires filter integrity test used in the processing of sterile solutions. Among several methods, we can decide to use bubble point test. We usually use vented GS-filters (Millipore co., USA) which are sterilizinggrade (0.22 um pore size) and are placed upper site on product vial. After the synthesis of $^{18}F$-FDG, solutions wet the membrane in filter and then go into the product vial. By all synthesis steps have finished, we can observe the presence of the bubbles in the product vial. Since we have started this study, we have never found any bubbles in the product vial. Because the maximum pressure intensity of the filter which has set by manufacturer is up to 5 bars, but helium gas pressure is up to 1 bar in our module system. So, we can make 5 bars pressure using helium gas bombe and increase pressure up to 5 bars step by step. However, it does not happen to anything in vial.

• Plant Biotechnology Reports
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• 제3권4호
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• pp.309-315
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• 2009

Molecular markers developed from the flanking sequences of two cytoplasmic male sterility (CMS)-associated genes, orf456 and ${\Psi}atp6-2$, have been used for marker-assisted selection of CMS in pepper. However, in practice, the presence of orf456 and ${\Psi}atp6-2$ at substoichiometric levels even in maintainer lines hampers reliable selection of plants containing the CMS gene. In this study, we developed a novel CMS-specific molecular marker, accD-U, for reliable determination of CMS lines in pepper, and used the newly and previously developed markers to determine the cytoplasm types of pepper breeding lines and germplasms. This marker was developed from a deletion in a chloroplast-derived sequence in the mitochondrial genome of a CMS pepper line. CMS pepper lines could be unambiguously determined by presence or absence of the accD-U marker band. Application of orf456, ${\Psi}atp6-2$and accD-U to various pepper breeding lines and germplasms revealed that accD-U is the most reliable CMS selection marker. A wide distribution of orf456, but not ${\Psi}atp6-2$, in germplasms suggests that the pepper cytoplasm containing both orf456 and ${\Psi}atp6-2$ has been selected as CMS cytoplasm from cytoplasm containing only orf456. Furthermore, factors other than orf456 may be required for the regulation of male sterility in pepper.

• 한국자원식물학회지
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• 제11권1호
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• pp.80-85
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• 1998

The influence of elevated CO2 and temperature on growth parameters, biomass production and its partitioning of rice (Oryza sativa L.cv. Chukwangbyeo) were investigated in the three experiments (1991-1993). Rice plants were grown from transplanting to harvest at either ambient(350ppm) or elevated CO2 concentrations (690 or 650ppm) in combination with either four or seven temperature regimes ranging form ambient temperature (AT) to AT plus 3$^{\circ}C$.From transplanting to panicle initiation, crop growth rate (CGR) was enhanced by up to 27% with elevated CO2 , primarily due to an an increase in leaf area index. although net assimilatiion rate was also greater at elevated CO2. The effect of elevated CO2 varied with temperature. During the reproductive phase, CGR declined linearly with increased temperature, and was greater at elevated CO2 . Elevated CO2 increased final crop biomass and panicle weight 30% respectively at AT(27.6$^{\circ}C$ : 1991) . However, there was no significant effect of elevated CO2 on panicle weight at AT plus 3$^{\circ}C$, where severe spikelet sterility occurred. There was no significant effect of elevated CO2 on panicle weight at AT plus 3$^{\circ}C$, where severe spikelet sterility occurred. There was also no effect of CO2 on biomass pratitioning into vegetative and reproductive organs (harvest index)) at AT, although higher temperature could affect that by inducing spikelet sterility. These results suggest that elevated CO2 could enhance rice producivity througth promoted growth and biomass production , but its positive effects may be less at higher temperatures.

• Clinics in Orthopedic Surgery
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• 제10권4호
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• pp.427-432
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• 2018

Background: The purpose of this study was to evaluate the usefulness of sonication technique for microbiological diagnosis and the sterility of the recycled autoclaved femoral components from infected total knee arthroplasty (TKA) using a sonication method. Methods: Nineteen femoral implants explanted from patients with infected TKA were sterilized with a standard autoclave method. Standard culture of the fluid before and after sonication of the sterilized implants was performed to detect pathogenic microorganisms. Additional experiments were performed to evaluate the sterility of the recycled implant by inducing artificial biofilm formation. Methicillin-resistant Staphylococcus aureus (MRSA) was inoculated into 10 implants and sterilization in a standard autoclave was performed, and then the fluid was cultured before and after sonication. Results: Two of the 19 sterilized implants were positive for growth of bacteria after sonication, whereas no growth was detected in the cultured fluid from the sterilized implants before sonication. The bacteria were Staphylococcus species in all two cases. In one of 10 implants inoculated with MRSA, the culture was positive for growth of bacteria both before and after sonication. However, Staphylococcus epidermidis was cultured from both occasions and thus this implant was thought to be contaminated. Conclusions: We found sonication for identification of pathogens could be helpful, but this finding should be interpreted carefully because of the possibility of contamination. Sterilization of an infected femoral implant with an autoclave method could be a good method for using the temporary articulating antibiotic spacer in two-stage revision arthroplasty.

• Journal of Ginseng Research
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• 제43권2호
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• pp.261-271
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• 2019

Background: Protopanaxatriol (PPT) is an aglycone of ginsenosides, which has high medicinal values. Production of PPT from natural ginseng plants requires artificial deglycosylation procedures of ginsenosides via enzymatic or physicochemical treatments. Metabolic engineering could be an efficient technology for production of ginsenoside sapogenin. For PPT biosynthesis in Panax ginseng, damarenediol-II synthase (PgDDS) and two cytochrome P450 enzymes (CYP716A47 and CYP716A53v2) are essentially required. Methods: Transgenic tobacco co-overexpressing P. ginseng PgDDS, CYP716A47, and CYP716A53v2 was constructed via Agrobacterium-mediated transformation. Results: Expression of the three introduced genes in transgenic tobacco lines was confirmed by Reverse transcription-polymerase chain reaction (RT-PCR). Analysis of liquid chromatography showed three new peaks, dammarenediol-II (DD), protopanaxadiol (PPD), and PPT, in leaves of transgenic tobacco. Transgenic tobacco (line 6) contained $2.8{\mu}g/g$ dry weight (DW), $7.3{\mu}g/g$ DW, and $11.6{\mu}g/g$ DW of PPT, PPD, and DD in leaves, respectively. Production of PPT was achieved via cell suspension culture and was highly affected by auxin treatment. The content of PPT in cell suspension was increased 37.25-fold compared with that of leaves of the transgenic tobacco. Transgenic tobacco was not able to set seeds because of microspore degeneration in anthers. Transmission electron microscopy analysis revealed that cells of phloem tissue situated in the center of the anther showed an abnormally condensed nuclei and degenerated mitochondria. Conclusion: We successfully achieved the production of PPT in transgenic tobacco. The possible factors deriving male sterility in transgenic tobacco are discussed.

• 한국작물학회지
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• 제30권4호
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• pp.359-367
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• 1985

본 시험은 산간부인 진안(해발 303 m)에서 생식생장기에 일반상수를 대비하여 냉수를 유수형성기와 감수분열기에 4, 8, 12 일간 관개하였을때 야기되는 물질생산 및 수용계의 냉해반응을 알고자 수행하였던 바 다음과 같은 결과를 얻었다. 1. 냉수관개기간이 길어질수록 간장, 수장, 추출도의 신장이 억제되었으며, 유수형성기 냉수관개는 하위절, 감수분열기 냉수관개는 상위절의 절간신장을 단축시켰다. 또한 지경 및 영화의 퇴화는 유수형성기 냉수 관개에 민감한 영향을 받아 2차지경과 영화의 분화착생수 감소 및 퇴화의 증가를 보였다. 2. 불임율과 등숙장해도 냉수관개기간이 길어질수록 심하였고 유수형성기보다 감수분열기 냉수관개에서 피해율이 현저히 높았는데 특히 피해반응이 예민한 시기는 영화원기분화기와 감수분열기경이었으며 불임율, 등숙비율과 수량과는 유의적인 상관관계를 보였다. 3. Source와 Sink 관련형질중 지경과 영화의 퇴화는 엽면적에 영향을 받고 불임율과 수량은 간장, 수장,추출도의 신장량과 상관이 있었다.