• Journal of Radiation Protection and Research
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• 제9권1호
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• pp.11-18
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• 1984

Well type 전리함과 미세전류 측정희로를 사용하여 표준 감마선 전리함 장치를 개발하였다. 미세전류는 automatic Townsend balance with stepwise compensation방법을 사용하여 측정하였다. $^{226}Ra$을 기준 선원으로 택하여 감마 방출핵종인 $^{241}Am,\;^{133}Ba,\;^{60}Co,\;^{134}Cs,\;^{137}Cs^{22}Na$에 대한 비교 교정인자를 산출하였으며, 감마에너지의 함수로서 검출 효율 곡선을 구하였다.

• 한국진공학회지
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• 제12권1호
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• pp.51-54
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• 2003

아르곤 방전 기체의 음 글로우 영역에서 자발적 직동의 광검류 신호를 관찰하였다. 자발적 진동의 광검규 신호는 3 mA 보다 낮은 땅선 전류에서 나타난다. 준안정 준위의 인자 밀도 변화와 1s-2p 전이의 방출 세기를 동시에 측정한 결과, 자발적 진동 신호를 발생시키는 메커니즘 중의 한 가지는 느린 전자의 충돌에 의한 준안정 준위 원자의 단계적 이온화이다.

• 암석학회지
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• 제10권3호
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• pp.133-147
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• 2001

최근 기존 연대측정법의 한계를 극복하여 절대연령 측정 대상을 획기적으로 넓힐 수 있는 방법으로 개발되고 있는 단계적 용해에 의한 연대측정법을 국내 시료에 적용하여 이 방법에 의한 연대측정의 성공가능성에 대해 평가하여 보았다. 대상 시료는 옥천변성대 대전 남부지역 창리층의 함우라늄 흑색 점판암, 영남육괴 북동부의 선캠브리아 내덕리 화강암에서 분리한 전기석 및 옥방광산의 회중석 등을 선정하였다. 이들에 대한 단계적 용해를 위해 산의 종류와 시간을 달리하여 점차 그 세기를 늘려가며 납을 용출시켰으며, 각 단계에서 얻은 용액들에 대해 납 등위원소 비율과 함께 납과 우라늄 함량을 열이온화 질량분석기로 측정하였 다. 그 결과 창리층 혹색 점판암과 내덕리 화강암으로부터는 상당한 Pb 동위원소 값의 분산을 얻을 수 있었으며 단계적 용해에 의한 Pb-Pb 연대측정이 성공적일 수 있음을 보여준다. 단계적 용해 과정에서 시료의 종류마다 우라늄과 납의 거동이 다르며 이는 구성 광물의 종류에 따라 우라늄과 납이 차지하는 결정 내에서의 위치에 지배되는 것으로 판단된다.

• Mass Spectrometry Letters
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• 제9권1호
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• pp.30-36
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• 2018

A quantitation method for free amino acids in human serum was developed using a stepwise-dilution method and a bimodal cation exchange (CEX)/hydrophilic interaction liquid chromatography (HILIC)-tandem mass spectrometry system equipped with an electrospray ionization source (ESI/MS/MS). This method, which was validated using quality control samples, was optimized for enhanced selectivity and sensitivity. Dithiothreitol (DTT) was used as a reducing agent to prevent the oxidation of a serum sample ($50{\mu}L$), which was then subjected to stepwise dilution using 3, 30, and 90 volumes of acetonitrile containing 0.1% formic acid. Chromatographic separation was performed on an Imtakt Intrada Amino Acid column ($50mm{\times}3mm$, $3{\mu}m$) in mixed mode packed with CEX and HILIC ligands embedded in the stationary phase. Underivatized free amino acids were eluted and separated within 10 min. As a result of the validation, the precision and accuracy for the inter- and intraday assays were determined as 2.11-11.51% and 92.82-109.40%, respectively. The lowest limit of quantification (LLOQ) was $0.5-4.0{\mu}g/mL$ and the matrix effect was 80.22-115.93%. The proposed method was successfully applied to the quantitative analysis of free amino acids in human serum.

• 한국환경농학회지
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• 제29권3호
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• pp.247-256
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• 2010

A high-performance liquid chromatographic (HPLC) method was developed to determine buprofezin residues in hulled rice and fruits. The buprofezin residue was extracted with acetone and the extract was stepwise purified by liquid-liquid partition and Florisil column chromatography. For rice samples, acetonitrile/n-hexane partition was additionally employed to remove nonpolar lipids. Reversed phase HPLC using an octadecylsilyl column was successfully applied to separate buprofezin from sample co-extractives, as detected by ultraviolet absorption at 250 nm. Recovery experiment at the limit of quantitation validated that the proposed method could evidently determine the buprofezin residue at the level of 0.02 mg/kg. Mean recoveries from hulled rice, apple, pear, and persimmon samples fortified at three tenfold levels were in the range of 80.8~85.2%, 89.1~98.4%, 88.8~95.7% and 90.8~96.2%, respectively. Relative standard deviations of the analytical method were all less than 5%, irrespective of sample types. A selected-ion monitoring LC/mass spectrometry with positive electrospray ionization was also provided to sensitively confirm the suspected residue.

• 대한전기학회논문지:전기물성ㆍ응용부문C
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• 제50권6호
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• pp.288-295
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• 2001

Predischarges in nonuniform electric field stressed by lightning impulse voltagesin SF(sub)6-$N_2$mixtures are initiated by streamer coronas. Due to field ehnancement at a protrusion point of electrodes new ionization processes occur and a precursor, which leads to a first leader, is created. The leader proceeds step by step to the opposite electrode and the final jump bridges the test gap. It was found that the predischarge is propagated with a leader mechanism of stepwise expansion from the predischarge current waveforms measured by a shunt. The predischarge current is closely related to the amplitude and polarity of applied voltages, the gas pressure and the gap geometry. The time intervals between step leaders for the positive and negative polarities were inversely proportional to V.P$^2$. When the gas pressure increases in the positive polarity, statistical time lag to statistical time lag to streamer corona inception increase slightly, but the formative time lag to flashover is significantly decreased.

• 농업과학연구
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• 제48권2호
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• pp.343-351
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• 2021

Screening was conducted using 200 kinds of plant extracts to explore herbicide-activated components of plant origin. We separated and purified active substances and elucidated chemical structures using Ligularia stenocephala M., which has strong activity and has not yet been studied. When the solvent fractions of the leaves of Ligularia stenocephala M. were tested for their herbicidal activity, ethyl acetate and chloroform layer showed an inhibition rate of 95.2% and 94.1%, respectively. In particular, the chloroform layer exerted more than 50% herbicidal activity at 10 ppm. From the chloroform layer with the highest herbicidal activity, we isolated three herbicidal active compounds using stepwise chromatography, specifically silica gel or octadecyl silica (ODS) column chromatography, Sep-pak cartridges, and high performance liquid chromatography (HPLC). Based on the analysis of the active compounds using electron ionization mass spectroscopy (EI-MS), ¹H-NMR, and ¹³C-NMR, we identified the active compounds as euparin, 5,6-dimethoxy-2-isopropenylbenzofuran, and liguhodgsonal. When the herbicidal activity of the identified compounds was tested, euparin showed selective herbicidal activity for lettuce at 10^-3 M, and both liguhogsonal and 5,6-dimethoxy-2-isoprophenylbenzofuran exerted selective activity for rice and Echinochloa crus-galli.