• Journal of Microbiology and Biotechnology
• /
• v.19 no.1
• /
• pp.65-71
• /
• 2009

Microbial oxidoreductive systems have been widely used in asymmetric syntheses of optically active alcohols. However, when reused in multi-batch reaction, the catalytic efficiency and sustainability of non-growing cells usually decreased because of continuous consumption of required cofactors during the reaction process. A novel method for NADPH regeneration in cells was proposed by using pentose metabolism in microorganisms. Addition of D-xylose, L-arabinose, or D-ribose to the reaction significantly improved the conversion efficiency of deracemization of racemic 1-phenyl-1,2-ethanediol to (S)-isomer by Candida parapsilosis cells already used once, which afforded the product with high optical purity over 97%e.e. in high yield over 85% under an increased substrate concentration of 15 g/l. Compared with reactions without xylose, xylose added to multi-batch reactions had no influence on the activity of the enzyme catalyzing the key step in deracemization, but performed a promoting effect on the recovery of the metabolic activity of the non-growing cells with its consumption in each batch. The detection of activities of xylose reductase and xylitol dehydrogenase from cell-free extract of C. parapsilosis made xylose metabolism feasible in cells, and the depression of the pentose phosphate pathway inhibitor to this reaction further indicated that xylose facilitated the NADPH-required deracemization through the pentose phosphate pathway in C. parapsilosis. moreover, by investigating the cofactor pool, the xylose addition in reaction batches giving more NADPH, compared with those without xylose, suggested that the higher catalytic efficiency and sustainability of C. parapsilosis non-growing cells had resulted from xylose metabolism recycling NADPH for the deracemization.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
• /
• v.14 no.4
• /
• pp.435-443
• /
• 2016

This paper describes the development of a dry operation process for PWR spent nuclear fuel, which is currently stored in the domestic NPP's storage pool, using a dual purpose metal cask. Domestic NNPs have had experience with wet type transportation of PWR spent nuclear fuel between neighboring NPPs since the early 1990s, but no experience with dry type operation. For this reason, we developed a specific operation process and also confirmed the safety of the major cask components and its spent nuclear fuel during the dual purpose metal cask operation process. We also describe the short term operation process that was established to be completed within 21 hours and propose the allowable working time for each step (15 hours for wet process, 3 hours for drain process and 3 hours for vacuum drying process).

• Animal cells and systems
• /
• v.4 no.3
• /
• pp.273-278
• /
• 2000

Transforming growth factor-$\beta$ (IGF-$\beta$)s are multifunctional small polypeptides synthesized in most cell types. TGF-$\beta$ exerts pivotal effects on both bone formation and resorption. In addition, increasing lines of evidence implicate TGF-$\beta$ as a potential coupling factor between these two processes during bone remodeling. In the present study, the expression form and the activation mechanism of latent-TGF-$\beta$ were investigated using specific antibodies for each isoform. TGF-$\beta$s were observed to be synthesized and accumulated in a large amount in cultured osteoblastic cells. The estimated molecular weights of intracellular TGF-$\beta$2 and -$\beta$3 were 49 and 55 kDa, respectively. Based on proteolytic digestion study and immunofluorescence observation, these precursor forms seemed to be accumulated in distinct intracellular compartments. To examine whether the internal pool of TGF-$\beta$ was possiblely regulated by external signals, their biological activites were examined in a conditioned media of this cell. Although the intact conditioned media did not contain detectable TGF-$\beta$ activity, heat-treatment or acid-activation of the conditioned media revealed significant TGF-$\beta$ activity. Furthermore, in the presence of estrogen, this activity was dramatically diminished. It is known that activation of latent TGF-$\beta$ can be achieved by different chemical and enzymatic treatments, or by incubation with certain cell types. This extracellular activation was suggested as a key step in the regulation of TGF-$\beta$ activity. In addition to these extracellular activation, this study suggests that the synthesis and intracellular processing are important regulation steps for TGF-$\beta$ action. In addition, this regulation Is specific for TGF-$\beta$ type 2, because the change was not observed in TGF-$\beta$3 in osteoblastic cell line.

• Journal of Korean Society on Water Environment
• /
• v.32 no.5
• /
• pp.475-491
• /
• 2016

The Changwon and Nam streams that run through the Changwon city were evaluated based on longitudinal and horizontal configurations and vegetation state in order to prepare a restoration plan. Riparian vegetation of the Changwon and Nam streams are normally dominated by herbaceous plants. However, the woody plants, Salix pseudolasiogyne, Rubus parvifolius, Salix koreensis, Robinia pseudoacacia, and Amorpha fruticosa have appeared. Planting to introduce various types of plants for landscape architecture has also been observed. Evaluation of naturalness based on the vegetation state of both Changwon and Nam streams showed very low naturalness of degree 1 or 2, with the exception of a few reaches of the upstream sector. In these few reaches of the upstream sector, Salix koreensis and Salix chaenomeloides showed heights at the subtree level, however, they were located at the position of shrubland close to the waterway. These reaches were, therefore, evaluated as degree 3 rather than degree 4 in naturalness. The result of diagnostic evaluation classified by reach indicated the requirement for active restoration, such as transformation of the channelized stream into a meandering one and a step-type cross section into a pool-type one, introduction of vegetation suitable to the region and site, and creation of an ecological network between the stream and the surrounding terrestrial ecosystems.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
• /
• v.8 no.1
• /
• pp.1-12
• /
• 1996

In lots of application to heat exchanger systems, closed two-phase thermosyphons are tilted from a horizontal. If the tilt angle, especially, is less than 30$^{\circ}$, the operational performances of thermosyphon are highly dependent on tilt angle. The present study was conducted to better understand such operational behaviors as mech-anni는 of phase change, and flow patterns inside a tilted thermosyphon. For experiment, an ethanol thermosyphon with a 35% of fill charge rate was designed and manufactured, using a copper tube with a diameter 19mm and a length 1500mm. Through a series of test, the tilt angle was kept constant at each of 4 different values in the range 10~25deg. and the heat supply to the evaporator was stepwisely increased up to 30㎾/$m^2$. When a steady state was established to the thermosyphon for each step of thermal loads, the wall temperature distribution and vapor temperature at the condenser were measured. The wall temperature distributions demonstrated a formation of dry patch in the top end zone of the evaporator, with a values of temperature 20~4$0^{\circ}C$ higher than the wetted surface for a moderate heat flux q≒20㎾/$m^2$. Inspite of the presence of hot dry patch, however, the mean values of boiling heat transfer coefficient at the evaporator wall were still in a good agreement with those predicted by Rohsenow's formula, which was based on nucleate boiling. For the condenser, the wall temperatures were practically uniform, and the measured values of condensation heat transfer coefficient were 1.7 times higher than the predicted values obtained from Nusselt's film condensation theory on tilted plate. Using those two expressions, a correlation was formulated as a function of heat flux and tilt angle, to determine the total thermal resistance of a tilted thermosyphon. The correlation formula showed a good agreement with the experimental data within 20%.

• Journal of Environmental Impact Assessment
• /
• v.19 no.2
• /
• pp.127-140
• /
• 2010

This study investigates the relation between the location of a habitat and the ecological connections according to the habitat type in the riparian zone at the Nonsan Stream and Yanghwa Stream. Stream habitat is classified into nine types for the aquatic insects and fish. For vegetation and birds, habitat is classified into two types of medium-scale streams, including both physical and chemical streams are analyzed accordingly. Nowadays, The fundamental goal of the river environment restoration enterprise is the rehabilitation or the restoration of the characteristic the river scenic or environment. For instance, The Channel habitats which has physical nature environment such as flat, speedy rapids, or closed-channel wetland, pool are constructed by artificial, Consequently, make them autogenesis smoothy eco-environment. However, the river environment are controlled not only physical environment, but it also need reasonable the quality of the water to compose smoothly. Finally, understanding what influence are effecting on physical habitat environment which are made by natural factors to water quality are very important factor for the river environment restoration enterprise Therefore, In this research, we are targeting to a basin to investigate the environment of the physical channel habitat and evaluate the changing of the water quality. This results will be a important characteristic that can judge the physical habitat and reciprocality connected to the water quality or adequacy of restoration technology. Therefore in this study, as a step to quantify functions and values of habitats and definite factors to perform habitat, we selected a representative stream of sand-stream, gravel-stream to classify habitat characteristics and quantified the physical, chemical, biological characteristics.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2018.05a
• /
• pp.479-479
• /
• 2018

하천의 수심, 유속, 하상경사, 하상재료, 만곡도 등과 같은 물리적 특성에 따라 생물서식처로서 다양한 특성을 나타내고 그 특성들에 의해 서식처의 유형을 구분할 수 있다. 이러한 서식처 유형에 따라 수중음향의 특성이 달라지며, 수중음향의 서식지의 양적 표시자로서 잠재력으로 인해 최근 많은 연구들이 시도되고 있다. 본 연구에서는 인공적인 영향을 적게 받은 자연하천으로 양양 남대천 12개 지점을 대상으로 하여 서식지의 형태에 따른 수중음향과 하천의 수리적 인자에 따른 관계를 파악하고자 하였다. 서식처 유형으로는 빠른 유속에 얕은 수심을 가지고 있는 여울(riffle), 수심이 깊고 유속이 느린 소(pool), 낙차가 있는 계단형 여울(step riffle)에 대해 조사하였다. 수중배경음은 여울과 계단형 여울이 유사하게 나타났으며 소는 상대적으로 낮은 배경음을 나타내었다. H2, H4, H5, H9, H10 지점은 여울 형태로 유속에 따라 H2, H5, H4, H9 지점 순으로 음압레벨이 낮아지는 경향을 보였으며, H10지점은 H9, H4 지점에 비해 낮은 유속임에도 높은 음압레벨을 보였다. 이는 H10 지점의 하상이 조도가 매우 큰 전석으로 이루어져 나타나는 현상으로 판단된다. H3, H6, H7, H8, H12 지점은 소 형태로 H12 지점을 제외하고 대체로 낮은 음압레벨을 보였으며, H3과 H6지점에서는 저주파수에서 높은 음압레벨을 보였다. 이는 수심이 깊은 경우 상류의 저주파수의 음파가 잘 전달되기 때문으로 판단된다. H1과 H11지점은 계단형 여울로 낮은 수심에서 빠른 유속을 가진 H11지점과 느린 유속을 가진 H1 지점에서 대체로 높은 음압레벨을 나타내었다. 이는 낙차에 의한 수중음향 발생에 의한 것으로 판단된다. 세 가지 유형에 따른 수중 음향을 비교하였을 때 음압레벨은 계단형 여울, 여울, 소 순서로 높게 나타났다. 결과적으로 수중음향이 서식처의 수리적 특성에 따라 다양한 특성을 나타내고 있으며, 이를 통해 생물서식처를 평가하기 위한 새로운 방법으로 수중음향의 활용 가능성을 제시하였다.

• Journal of Korea Trade
• /
• v.26 no.3
• /
• pp.23-44
• /
• 2022

Purpose - Recent issues such as vessel enlargement, strengthening of environmental regulations, and port smartization are expected to increase costs and intensify competition in the port industry. In the new normal era, when external growth has reached its limit, the efficient operation of ports is becoming indispensable for achieving sustainable growth. This study aims to identify the determinants of inefficiency by examining the cost structure and efficiency of container terminals in Korea and furthermore propose the political implications to derive the maximization of efficiency. Design/methodology - This study estimates the cost function of container terminal operators and identifies the efficiency of container terminals using stochastic cost frontier (SCF) in the first stage. In the second step, the SCF results are compared with the data envelopment analysis (DEA). Last, this paper proposes efficiency determinants on container terminal operation to establish appropriate strategies. Out of the 29 container terminal operators in South Korea, 13 operators participated in the survey. The translog cost function was estimated utilizing a total of 116 observations collected over the 2007-2017 period. Findings - Empirical analysis shows that economies of scale exist in Korea's container ports, which provides a rationale for the government's policy to establish the global terminal operator by integrating small terminal operators to enhance competitiveness. In addition, as a result of the determinants analysis, container throughput, weight of direct employment costs, and labour cost share have positive effects on improving cost efficiency, while inefficiency increases as the length of quay increases. More specifically, cost efficiency improves as the proportion of direct employment costs to outsourcing service costs increases. Originality/value - This study contributes to analyzing the inefficiency factors of container terminals through efficiency analysis with respect to a cost function. In addition, this study proposes the practical and political implications, such as establishing a long-term manpower pool, the application of the hybrid liner terminal system, and the construction of a statistical data system, to improve the cost inefficiency of terminal operators.

• Korean Journal of Microbiology
• /
• v.50 no.1
• /
• pp.1-7
• /
• 2014

Site-specific incorporation of unnatural amino acids (SSIUA) into protein can be achieved in vivo by coexpression of an orthogonal pair of suppressor tRNA and engineered aminoacyl-tRNA synthetase (ARS) that specifically ligates an unnatural amino acid to the suppressor tRNA. As a step to develop the SSIUA technique in Escherichia coli, here we established a new 2-step screening system that can be used for selecting an ARS variant(s) that ligates an unnatural amino acid to a suppressor tRNA. A positive selection system consists of chloramphenicol acetyl transferase gene containing an amber mutation at the $27^{th}$ residue, and efficiently concentrated amber suppressible ARS with a maximum enrichment factor of $9.0{\times}10^5$. On the other hand, a negative selection system was constructed by adding multiple amber codons in front of a lethal gene encoding the control of cell death B toxin (ccdB) which acts as an inhibitory protein of bacterial topoisomerase II. Amber suppression of ccdB by an orthogonal pair of Saccharomyces cerevisiae tyrosyl-tRNA synthetase (TyrRS) and an amber suppressor tRNA significantly inhibits bacterial growth. This selection system was also able to efficiently remove amber suppressible ARS which could ligate natural amino acids to the suppressor tRNA. Thus, sequential combination of these two selection systems might be able to function as a powerful tool for selecting an ARS variant that specifically ligates an unnatural amino acid to the suppressor tRNA from an ARS mutant pool.

• Journal of Korean Society of Rural Planning
• /
• v.29 no.3
• /
• pp.25-37
• /
• 2023

According to the 「Rural Spatial Reconstruction and Regeneration Support Act」, enacted on March 29, 2024, all local governments are required to establish a 'Rural Spatial Reconstruction and Regeneration Plan' (hereinafter referred to as the 'Rural Spatial Plan'). In order for the 'Rural Spatial Plan' to be appropriately established, this study analyzed the supply and demand of spatial data from the perspective of user stakeholders and derived implications for improving rural spatial planning data utilization. In conclusion, three key recommendations come from this result. Firstly, it is necessary to establish an integrated DB for rural spatial planning data. This can solve the problem of low awareness of scattered data-providing websites, reduce the processing time of non-GIS data, and reduce the time required to acquire data by securing the availability of data search and download. In particular, research should be conducted on the establishment of a spatial analysis simulation system to support stakeholders' decision-making, considering that many stakeholders have difficulty in spatial analysis because spatial analysis techniques were not actively used in rural projects before the implementation of the rural agreement system in 2020. Secondly, research on how to improve data acquisition should be conducted in each data sector. The data sector group with the lowest ease of receiving are 'Local Community Domain', 'Changes in Domestic and International Conditions', and 'Provision and Utilization of Daily Life Services'. Lastly, in-depth research is needed on how to raise each rural spatial planning data supply stakeholder to the position of player. Stakeholders of 'University Institutions' and 'Public Enterprises and Research Institutes' should give those who participate in the formulation of rural spatial plans access to the raw data collected for public work. Stakeholders of 'Private company' need to come up with realistic measures to build a data pool centered on consultative bodies between existing private companies and then prepare a step-by-step strategy to fully open it by participating various stakeholders. In order to induce 'Village Residents and Associations' stakeholders to play a leading role as owners and producers of data, personnel should be trained to collect and record data related to the village. In addition, support measures should be prepared to continue these activities.