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Combination Culture of Rotifer Brachionus rotundiformis and Copepod Apocylops sp. (로티퍼 Brachionus rotundiformis와 코페포다 Apocyclops sp.의 혼합 배양)

  • Jung, Min-Min;Rho, Sum
    • Journal of Aquaculture
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    • v.11 no.4
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    • pp.449-455
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    • 1998
  • The small size food organism(under the size 150${\mu}m$) is needed as food for early stage of marine fish larvae of small mouse (e.g the group of grouper). This study was investigated to develop a method for copepod Apocyclops sp. culture in combination with the rotifer B. rachionus for stable culture of copepod species and harvest of various size food organisms. The culture conditions as temperature, salinity, culture volume, photo period, culture preiod and observation interval were 25${\circ}C$, 22ppt, 40ml, all dark except to observation time, 16 days and every two day during the experimental period, respectively. The Tetraselmis suecica was used as the food for the two testing orgtanisms. After every two day counting, theses two organisms were transferred to fresh culture tanks with Tetraselmis suecica of $7{\times}10^5$cells/ml. In the mixed culture of B. rotundiformis and A. sp., growth of rotifer was suppressed by mixed culture with A. sp. whereas the growth of copepod Apocyclops was promoted in the mixed culture with rotifer B. rotundiformis (the maximum density was 22 individuals/ml through the 16 culture days). Moreover, the number of copepod nauplius were promoted about 2 times in the mixed culture compared to the numbers in single species culture. With this combination culture, the havested two food organisms of variable sizes. This size variation of food organisms was useful tools for larval rearing of small mouse marine fish larvae and next step food organism size of post hatched larvae.

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The Microleakage in Class V Cavity Based on Different Kinds of Several Dentin Adhesive and Composite Resin (5급 와동에서 수종의 상아질 접착제와 복합레진의 종류에 따른 미세누출)

  • Choi, Yoo-Seok;Jeong, Soon-Jeong;Jeong, Moon-Jin;Ahn, Yong-Soon;Park, Seon-Nyeo;Lim, Do-Seon
    • Journal of dental hygiene science
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    • v.13 no.3
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    • pp.330-338
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    • 2013
  • In order to explore microleakage in class V cavity based on different kinds of several dentin adhesive and composite resin, 2 kinds of composite resin was restored and exposed after applying 4 kinds of dentin adhesives. Deposited in methylene blue solution for 4 hours and cut in parallel with tooth longitudinal axis. By observing dye penetration level of enamel and dentin margins of each restored resin following conclusion was obtained. 1. In composite resin Filtek Z350XT Universal (3M/ESPE Dental Products, USA) in enamel margin, Easy Bond (3M/ESPE Dental Products) showed the lowest microleakage and this leakage was represented to be high in the order of Single Bond 2 (3M/ESPE Dental Products), Scotchbond Multi-Purpose (3M/ESPE Dental Products) and Cearfil SE Bond (Kuraray Medical Inc., Japan). In case of Filtek Z350XT Flowable (3M/ESPE Dental Products), Scotchbond Multi-Purpose showed the lowest microleakage and this leakage was represented to be high in the order of Single Bond 2, Clearfil SE Bond and Easy Bond. 2. In case of Filtek Z350XT Universal in dentin margin, Easy Bond showed the lowest microleakage and this leakage was represented to be high in the order of Scotchbond Multi-Purpose, Single Bond 2 and Clearfil SE Bond. In case of Filtek Z350XT Flowable, Scotchbond Multi-Purpose and Single Bond showed the lowest microleakage and this leakage was represented to be high in the order of Clearfil SE Bond and Easy Bond. 3. In all the groups excepting S-U group (Single Bond 2+Filtek Z350XT Universal), enamel margin showed more higher microleakage than that of dentin margin. 4. There was a difference between enamel and dentin margin among each group but it was not significant statistically (p>0.05). When summarizing this result, it is considered that composite resin and dentin adhesive could be applied selectively and particularly in case of applying 1-step self-etching dentin adhesive, this method would be advantageous for manipulation convenience and shortening of operation time.

Analysis and Prediction of Sewage Components of Urban Wastewater Treatment Plant Using Neural Network (대도시 하수종말처리장 유입 하수의 성상 평가와 인공신경망을 이용한 구성성분 농도 예측)

  • Jeong, Hyeong-Seok;Lee, Sang-Hyung;Shin, Hang-Sik;Song, Eui-Yeol
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.3
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    • pp.308-315
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    • 2006
  • Since sewage characteristics are the most important factors that can affect the biological reactions in wastewater treatment plants, a detailed understanding on the characteristics and on-line measurement techniques of the influent sewage would play an important role in determining the appropriate control strategies. In this study, samples were taken at two hour intervals during 51 days from $1^{st}$ October to $21^{st}$ November 2005 from the influent gate of sewage treatment plant. Then the characteristics of sewage were investigated. It was found that the daily values of flow rate and concentrations of sewage components showed a defined profile. The highest and lowest peak values were observed during $11:00{\sim}13:00$ hours and $05:00{\sim}07:00$ hours, respectively. Also, it was shown that the concentrations of sewage components were strongly correlated with the absorbance measured at 300 nm of UV. Therefore, the objective of the paper is to develop on-line estimation technique of the concentration of each component in the sewage using accumulated profiles of sewage, absorbance, and flow rate which can be measured in real time. As a first step, regression analysis was performed using the absorbance and component concentration data. Then a neural network trained with the input of influent flow rate, absorbance, and inflow duration was used. Both methods showed remarkable accuracy in predicting the resulting concentrations of the individual components of the sewage. In case of using the neural network, the predicted value md of the measurement were 19.3 and 14.4 for TSS, 26.7 and 25.1 for TCOD, 5.4 and 4.1 for TN, and for TP, 0.45 to 0.39, respectively.

The Generation of Westerly Waves by Sobaek Mountains (소백산맥에 의한 서풍 파동 발생)

  • Kim, Jin wook;Youn, Daeok
    • Journal of the Korean earth science society
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    • v.38 no.1
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    • pp.24-34
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    • 2017
  • The westerly waves generation is described in the advanced earth science textbook used at high school as follows: as westerly wind approaches and blows over large mountains, the air flow shows wave motions in downwind side, which can be explained by the conservation of potential vorticity. However, there has been no case study showing the phenomena of the mesoscale westerly waves with observational data in the area of small mountains in Korea. And thus the wind speed and time persistency of westerly winds along with the width and length of mountains have never been studied to explain the generation of the westerly waves. As a first step, we assured the westerly waves generated in the downwind side of Sobaek mountains based on surface station wind data nearby. Furthermore, the critical or minimum wind velocity of the westerly wind over Sobaek mountains to generate the downwind wave were derived and calcuated tobe about $0.6m\;s^{-1}$ for Sobaek mountains, which means that the westerly waves could be generated in most cases of westerly blowing over the mountains. Using surface station data and 4-dimensional assimilation data of RDAPS (Regional Data Assimilation and Prediction System) provided by Korea Meteorological Agency, we also analyzed cases of westerly waves occurrence and life cycle in the downwind side of Sobaek mountains for a year of 2014. The westerly waves occurred in meso-${\beta}$ or -${\gamma}$ scales. The westerly waves generated by the mountains disappeared gradually with wind speed decreasing. The occurrence frequency of the vorticity with meso-${\beta}$ scale got to be higher when the stronger westerly wind blew. When we extended the spatial range of the analysis, phenomena of westerly waves were also observed in the downwind side of Yensan mountains in Northeastern China. Our current work will be a study material to help students understand the atmospheric phenomena perturbed by mountains.

Consideration of density matching technique of the plate type direct radiologic image system and the conventional X-ray film;first step for the subtraction (Ektaspeed plus 필름을 이용한 일반 방사선시스템과 Digora를 이용한 디지탈 영상시스템의 밀도변화 비교연구)

  • So, Sung-Soo;Noh, Hyeun-Soo;Kim, Chang-Sung;Choi, Seong-Ho;Kim, Kee-Deog;Cho, Kyoo-Sung
    • Journal of Periodontal and Implant Science
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    • v.32 no.1
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    • pp.199-211
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    • 2002
  • Digital substraction technique and computer-assisted densitometirc analysis detect minor change in bone density and thus increase the diagnostic accuracy. This advantage as well as high sensitivity and objectivity which precludes human bias have drawn interest in radiologic research area. The objectives of this study are to verify if Radiographic density can be recognized in linear pattern when density profile of standard periapical radiograph with the aluminium stepwedge as the reference, was investigated under varies circumstances which can be encountered in clinical situations, and in addition to that to obtain mutual relationship between the existing standard radiographic system, and future digital image systems, by confirming the corelationship between the standard radiograph and Digora system which is a digital image system currently being used. In order to make quantitative analysis of the bone tissue, digital image system which uses high resolution automatic slide scanner as an input device, and Digora system were compared and analyzed using multifunctional program, Brain3dsp. The following conclusions were obtained. 1. Under common clinical situation that is 70kVp, 0.2 sec., and focal distance 10cm, Al-Equivalent image equation was found to be Y=11.21X+46.62 $r^2=0.9898$ in standard radiographic system, and Y=12.68X+74.59, $r^2=0.9528$ in Digora system, and linear relation was confirmed in both the systems. 2. In standard radiographic system, when all conditions were maintained the same except for the condition of developing solution, Al-Equivalent image equation was Y=10.07X+41.64, $r^2=0.9861$ which shows high corelationship. 3. When all conditions were maintained the same except for the Kilovoltage peak, linear relationship was still maintained under 60kVp, and Al-Equivalent image equation was Y=14.60X+68.86, $r^2=0.9886$ in the standard radiograhic system, and Y=13.90X+80.68, $r^2=0.9238$ in Digora system. 4. When all conditions were maintained the same except for the exposure time which was varied from 0.01 sec. to 0.8 sec., Al-Equivalent image equation was found to be linear in both the standard radiographic system and Digora system. The R-square was distributed from 0.9188 to 0.9900, and in general, standard radiographic system showed higher R-square than Digora system. 5. When all conditions were maintained the same except for the focal distance which was varied from 5cm to 30cm, Al-Equivalent image equation was found to be linear in both the standard radiographic system and Digora system. The R-square was distributed from 0.9463 to 0.9925, and the standard radiographic system had the tendency to show higher R-square in shorter focal distances.

Dynamic Behavior of Reactor Internals under Safe Shutdown Earthquake (안전정기지진하의 원자로내부구조물 거동분석)

  • 김일곤
    • Computational Structural Engineering
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    • v.7 no.3
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    • pp.95-103
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    • 1994
  • The safety related components in the nuclear power plant should be designed to withstand the seismic load. Among these components the integrity of reactor internals under earthquake load is important in stand points of safety and economics, because these are classified to Seismic Class I components. So far the modelling methods of reactor internals have been investigated by many authors. In this paper, the dynamic behaviour of reactor internals of Yong Gwang 1&2 nuclear power plants under SSE(Safe Shutdown Earthquake) load is analyzed by using of the simpled Global Beam Model. For this, as a first step, the characteristic analysis of reactor internal components are performed by using of the finite element code ANSYS. And the Global Beam Model for reactor internals which includes beam elements, nonlinear impact springs which have gaps in upper and lower positions, and hydrodynamical couplings which simulate the fluid-filled cylinders of reactor vessel and core barrel structures is established. And for the exciting external force the response spectrum which is applied to reactor support is converted to the time history input. With this excitation and the model the dynamic behaviour of reactor internals is obtained. As the results, the structural integrity of reactor internal components under seismic excitation is verified and the input for the detailed duel assembly series model could be obtained. And the simplicity and effectiveness of Global Beam Model and the economics of the explicit Runge-Kutta-Gills algorithm in impact problem of high frequency interface components are confirmed.

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Expression of EGFR in Non-small Cell Lung Cancer and its Effects on Survival (비소세포 폐암에서 EGFR의 발현률과 생존률에 미치는 영향)

  • Kim, Hak-Ryul;Jeong, Eun-Taik
    • Tuberculosis and Respiratory Diseases
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    • v.44 no.6
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    • pp.1285-1295
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    • 1997
  • Background : EGFR is one of the initial step in signal transduction pathway about multistep carcinogenesis. It is homologous to oncogene erbB-2 and is the receptor for EGF and TGF alpha. EGFR has important role in the growth and differentiation of tumor cells. So, EGFR in non-small cell lung cancer was examined to search for possible evidence as clinical prognostic factor. Methods : To investigate the role of EGFR in lung cancer, the author performed immunohistochemical stain of EGFR on 57 resected primary non-small cell lung cancer specimens. And the author analyzed the correlation between EGFR expression, clinical parameters, Sand $G_1$ phase fraction and survival. Results : 1) EGFR were detected in 56% of total 57 patients (according to histologic type, squamous cancer 50%, adenocarcinoma 63%, large cell cancer 75%) (according to TNM stage, stage I 64%, stage II 38%, stage III 55%) (according to cellular differentiation, well 50%, moderately 52%, poorly 65%). All differences were insignificant 2) Using the flow cytometric analysis, mean S-phase fraction of EGFR (+) and (-) group were 22.3(${\pm}10.5$)%. 18.0(${\pm}10.9$)% (p>0.05), mean $G_1$-phase fraction of EGFR (+) and (-) group were 68.4(${\pm}11.6$)%, 71.1(${\pm}12.8$)%, (p>0.05) 3) Two-year survival rate of EGFR (+) and (-) group were 53%, 84%, median survival time of EGFR (+) and (-) group were 26, 53 months. (p<0.05, Kaplan-Meier, generalized Wilcox) Conclusion : EGFR immunostaining may be a simple and useful method for survival prediction in non-small cell lung cancer.

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Tendency of 'a Cartoon Image' Appearing in Korean Modern Fine Arts - Ocusing on 'Atomouse' of Lee Dong-gi - (한국 현대 미술에 나타나는 '만화 이미지'의 경향성 -이동기의 <아토마우스>를 중심으로-)

  • Jeon, Young Je
    • Cartoon and Animation Studies
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    • s.36
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    • pp.669-702
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    • 2014
  • Today, in Korean modern fine arts, 'cartoon images' are utilized for their roles of the 'personas' of the artists, and as the 'texts' for the artistic discourses, while they play a role as a bridgehead between the public and art. In this study, we made a study, focusing on 'Atomouse' of Lee Dong-gi, in order to examine how the borrowing of the 'cartoon images' was made in the Korean modern fine arts, and what its period context is. 'Atomouse' of Lee Dong-gi is the first cartoon character that appeared in Korean modern fine arts, and it has acquired a symbolism of a subculture being acknowledged as art. In this study, we tried to find out what period contexts the changes of 'Atomouse' have, focusing on the private exhibitions of Lee Dong-gi, such as 'Smoking' Exhibition (2006), 'Bubble' Exhibition (2008), 'Double Vision' Exhibition (2008), 'The Garden of Uncertainty' Exhibition (2012) and 'Do Not Look Back with Angry Face' Exhibition (2013). Atomouse was born in the era of 'lack of pop' a change was attempted of it in the era of 'excess of pop' and its proceeding ended along with the 'settlement of the neo-pop'. From Atomouse started from the unconsciousness of the artist, we can find the 'identity' of the Republic of Korea, which was being influenced by the 'American and Japanese' culture, as well as the symbolism of a subculture being acknowledged as art, while it emerged as an icon to represent the Korean pop art of the time. Then, as the agony and self-examination of the artist was contained in it, its use was changed into the role as a persona and it was utilized as a tool to connect the and figurative worlds. In the end, the artist put an end to the proceeding of the 'Atomouse' at the boundaries of a persona and an alter ego, removing the creation with his own hands. In the process in which Lee Dong-gi created and changed 'Atomouse', the start-up and growth of the pop art are included in the history of the Korean modern fine arts, apart from a study of an artist. 'Atomouse' was not only painted on the walls of the subway stations in the form of public art, but it tried to closely communicate with the public through the collaboration with diverse media. It made the best use of the 'marketability' which a 'cartoon character' had at first, so that pure art was made a step closer to the public. So 'Atomouse' of Lee Dong-gi not only raised a subculture into the realm of high art, but lowered the door sill to high art. Through the study we could confirm what position 'Atomouse' of Lee Dong-gi, which started absurdly between parody and Hommage, takes in Korean modern art of today.

Effect of moisture on sealing ability of root canal filling with different types of sealer through the glucose penetration model (수종의 실러를 이용한 근관 충전 시 근관 내 수분이 치근단 폐쇄효과에 미치는 영향)

  • Jang, Jin-Ah;Kim, Hee-Lyang;Her, Mi-Ja;Lee, Kwang-Won;Yu, Mi-Kyung
    • Restorative Dentistry and Endodontics
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    • v.35 no.5
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    • pp.335-343
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    • 2010
  • Objectives: To compared the effect of different levels of moisture of root canal on the sealing ability after filling with four different types of sealer. Materials and Methods: Single-rooted teeth (n = 90) instrumented to and apical size of 0.06 / 45 were randomly assigned to 12 experimental groups (n = 7 per group), positive/negative control groups (n = 3 per group). The teeth of the experimental groups (a. DRY; b. PAPER POINT DRY; c. WET) were obturated with sealer (Group 1-3: Sealapex; Group 4-6: AH plus; Group 7-9: Tubuli-seal; Group 10-12: EndoRez) and warm vertical compaction method. After 7 days in $37^{\circ}C$, 100% humidity, the coronal-to-apical microleakage was evaluated quantitatively using a glucose leakage model. The leaked glucose concentration was measured with spectrophotometer at 1, 3, 7, 14, 21, and 30 days. Data were recorded ad mmol/L and statistically analysed with the two-way ANOVA and Duncan test (p = 0.05). Results: Throughout the experimental period Tubuli-seal/WET (Group 9) showed the highest mean cumulative glucose penetration (178.75 mmol/L), whereas AH plus/DRY (Group 4) had the least (20.78 mmol/L). Conclusions: The results of this study demonstrated that the moisture condition of root canals at the time of obturation and the type of sealer that was used had a significant effect on leakage and sealing ability. Thus drying procedure according to sealer types is a critical step and should not be missed in endodontic treatment.

Ecoclimatic Map over North-East Asia Using SPOT/VEGETATION 10-day Synthesis Data (SPOT/VEGETATION NDVI 자료를 이용한 동북아시아의 생태기후지도)

  • Park Youn-Young;Han Kyung-Soo
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.8 no.2
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    • pp.86-96
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    • 2006
  • Ecoclimap-1, a new complete surface parameter global database at a 1-km resolution, was previously presented. It is intended to be used to initialize the soil-vegetation- atmosphere transfer schemes in meteorological and climate models. Surface parameters in the Ecoclimap-1 database are provided in the form of a per-class value by an ecoclimatic base map from a simple merging of land cover and climate maps. The principal objective of this ecoclimatic map is to consider intra-class variability of life cycle that the usual land cover map cannot describe. Although the ecoclimatic map considering land cover and climate is used, the intra-class variability was still too high inside some classes. In this study, a new strategy is defined; the idea is to use the information contained in S10 NDVI SPOT/VEGETATION profiles to split a land cover into more homogeneous sub-classes. This utilizes an intra-class unsupervised sub-clustering methodology instead of simple merging. This study was performed to provide a new ecolimatic map over Northeast Asia in the framework of Ecoclimap-2 global database construction for surface parameters. We used the University of Maryland's 1km Global Land Cover Database (UMD) and a climate map to determine the initial number of clusters for intra-class sub-clustering. An unsupervised classification process using six years of NDVI profiles allows the discrimination of different behavior for each land cover class. We checked the spatial coherence of the classes and, if necessary, carried out an aggregation step of the clusters having a similar NDVI time series profile. From the mapping system, 29 ecosystems resulted for the study area. In terms of climate-related studies, this new ecosystem map may be useful as a base map to construct an Ecoclimap-2 database and to improve the surface climatology quality in the climate model.