• Title/Summary/Keyword: Step Parameter

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Crystallization and Preliminary X-Ray Crystallographic Analysis of UDP-N-Acetylglucosamine Enolpyruvyl Transferase from Haemophilus influenzae in Complex with UDP-N-Acetylglucosamine and Fosfomycin

  • Yoon, Hye-Jin;Ku, Min-Je;Ahn, Hyung Jun;Lee, Byung Il;Mikami, Bunzo;Suh, Se Won
    • Molecules and Cells
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    • v.19 no.3
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    • pp.398-401
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    • 2005
  • The bacterial enzyme UDP-N-acetylglucosamine enolpyruvyl transferase catalyzes the first committed step of peptidoglycan biosynthesis, i.e., transfer of enolpyruvate from phosphoenolpyruvate to UDP-N-acetyl-glucosamine. We have overexpressed the enzyme from Haemophilus influenzae in Escherichia coli and crystallized it in the apo-form, as well as in a complex with UDP-N-acetylglucosamine and fosfomycin using ammonium sulfate as the precipitant. X-ray diffraction data from a crystal of the apo-form were collected to $2.8{\AA}$ resolution at 293 K. The crystal quality was improved by co-crystallization with UDP-N-acetylglucosamine and fosfomycin. X-ray data to $2.2{\AA}$ have been collected at 100 K from a flash-frozen crystal of the complex. The complex crystals belong to the orthorhombic space group I222 (or $I2_12_12_1$) with unit-cell parameters of a = 63.7, b = 124.5, and $c=126.3{\AA}$. Assuming a monomer of the recombinant enzyme in the crystallographic asymmetric unit, the calculated Matthews parameter ($V_M$) is $2.71{\AA}^3Da^{-1}$ and solvent content is 54.6%.

Effect of KCN Treatment on Cu-Se Secondary Phase of One-step Sputter-deposited CIGS Thin Films Using Quaternary Target

  • Jung, Sung Hee;Choi, Ji Hyun;Chung, Chee Won
    • Current Photovoltaic Research
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    • v.2 no.3
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    • pp.88-94
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    • 2014
  • The structural, optical and electrical properties of sputter-deposited CIGS films were directly influenced by the sputtering process parameters such as substrate temperature, working pressure, RF power and distance between target and substrate. CIGS thin films deposited by using a quaternary target revealed to be Se deficient due to Se low vapor pressure. This Se deficiency affected the overall stoichiometry of the films, causing the films to be Cu-rich. Current tends to pass through the Cu-Se channels which act as the shunting path increasing the film conductivity. The crystal structure of CIGS thin films depends on the substrate orientation due to the influence of surface morphology, grain size and stress of Mo substrate. The excess of Cu was removed from the CIGS films by KCN treatment, achieving a suitable Cu concentration (referred as Cu-poor) for the fabrication of solar cell. Due to high Cu concentrations on the CIGS film surface induced by Cu-Se phases after CIGS film deposition, KCN treatment proved to be necessary for the fabrication of high efficiency solar cells. Also during KCN treatment, dislocation density and lattice parameter decreased as excess Cu was removed, resulting in increase of bandgap and the decrease of conductivity of CIGS films. It was revealed that Cu-Se secondary phase could be removed by KCN wet etching of CIGS films, allowing the fabrication of high efficiency absorber layer.

A Disturbance Observer-Based Robust Controller Against Load Variations in a Single Phase DC/AC Inverter System (단상 DC/AC 인버터 시스템의 부하변동을 고려한 외란 관측기 기반 제어기)

  • Kim, Sung-Jong;Jeong, Yu-Seok;Son, Young-Ik
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.44 no.4 s.316
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    • pp.21-26
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    • 2007
  • Output voltage waves of a DC/AC inverter system are likely to be distorted if variable loads e.g. motors or rectifiers exist in the output terminal. This paper designs a disturbance observer-based PI-controller for a single-phase inverter system that is robust against load changes. In this Paper, we regard the output voltage changes due to various loads as disturbances of the control system. Then we design a disturbance observer for estimation of the disturbances caused by the load current and any other error sources (such as parameter uncertainties and model mismatches etc.). In order to test the performance of the proposed control law, simulation studies are carried out for a single-phase inverter system using SimPowerSystem of Matlab Simuink. Compared to a simple PI-control, the disturbance observer-based controller shows enhanced performance in transient responses for step load changes.

Noise Modeling for CR Images of High-strength Materials (고강도매질 CR 영상의 잡음 모델링)

  • Hwang, Jung-Won;Hwang, Jae-Ho
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.45 no.5
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    • pp.95-102
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    • 2008
  • This paper presents an appropriate approach for modeling noise in Computed Radiography(CR) images of high strength materials. The approach is specifically designed for types of noise with the statistical and nonlinear properties. CR images Ere degraded even before they are encoded by computer process. Various types of noise often contribute to contaminate radiography image, although they are detected on digitalization. Quantum noise, which is Poisson distributed, is a shot noise, but the photon distribution on Image Plate(IP) of CR system is not always Poisson process. The statistical properties are relative and case-dependant due to its material characteristics. The usual assumption of a distribution of Poisson, binomial and Gaussian statistics are considered. Nonlinear effect is also represented in the process of statistical noise model. It leads to estimate the noise variance in regions from high to low intensity, specifying analytical model. The analysis approach is tested on a database of steel tube step-wedge CR images. The results are available for the comparative parameter studies which measure noise coherence, distribution, signal/noise ratios(SNR) and nonlinear interpolation.

Suggestion of Charts and Equations Estimating the Strength Parameters of Rock Mass Using the Rock Mass Classification Value (RMC 값을 이용한 암반의 강도정수 값 추정도표 및 추정식의 제안)

  • Kim, Min-Kwon;Lee, Yeong-Saeng
    • Journal of the Korean Geotechnical Society
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    • v.30 no.3
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    • pp.73-85
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    • 2014
  • The strength parameters used in rock mass design are mainly estimated by equations using Hoek-Brown failure criterion because the tests to obtain the values are limited and expensive. To estimate the strength parameters, the Hoek-Brown failure criterion should be transformed to the Mohr-Coulomb failure criterion. But the processes are more or less cumbersome due to the several stages including the computation and the analyzing steps. In this study, several rock states of various conditions were modeled and then the strength parameters were estimated using the Hoek-Brown failure criterion. Thereafter by analyzing the results, some charts and equations estimating the strength parameters through only one step or easily in the field using the values of RMC, the uniaxial compressive strength and the rock constant ($m_i$), were suggested. And then the suggested method was compared and discussed with the existing method.

Jeju 80kV HVDC Controller Modeling Using PSCAD/EMTDC Program (PSCAD/EMTDC 프로그램을 이용한 제주 80kV HVDC 제어기 모델링)

  • Choi, Soon-Ho;Lee, Seong-Doo;Kim, Chan-Ki
    • The Transactions of the Korean Institute of Power Electronics
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    • v.16 no.6
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    • pp.533-541
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    • 2011
  • This paper studies modeling of Jeju 80kV HVDC system and its controller by using PSCAD/EMTDC program. Reduced ac network is applied to verify interaction between ac network and dc system. Design parameter is applied to the converter transformer, harmonic filter and dc transmisstion line to simulate dc system. HVDC controller is divided into a rectifier controller and a inverter controller according to the converter operating mode. The inverter controller is composed of current control, voltage control and extingtion angle control. The rectifier controller is composed of current control and voltage control. Both controller has VDCOL characteristics so that current order is dependant on voltage variation. Step response, ac network single phase fault, three phase fault is simulated to verify the dynamic performance of controller model in both transient state and steady state.

Correlation Analysis Between Vocal Fold Vibration and Voice Signal Analysis Parameter by Water Temperature (수온에 따른 성대 진동과 음성신호 분석 요소간의 상관성 분석)

  • Kim, Bong-Hyun;Cho, Dong-Uk
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.37 no.4C
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    • pp.347-353
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    • 2012
  • In this paper, we carried out experiments to analyze influence of vocal cords according to changes of water temperature. We would like to particularly perform a study to design voice measurement system for significant extraction about vibration patterns of vocal cords according to temperature changes of water to drink. To this end, we measured elements value of voice analysis vibration of vocal cords to change, when drank, temperature difference of step 8 from $0^{\circ}C$ to $70^{\circ}C$ to $10^{\circ}C$ intervals. As a result of us experiment, when drank water of $30^{\circ}C{\sim}40^{\circ}C$, vibration of vocal cords stabilized and accuracy of pronunciation improved. We can analyzed that water of $30^{\circ}C{\sim}40^{\circ}C$ had a good effect in vocal cords.

A hybrid self-adaptive Firefly-Nelder-Mead algorithm for structural damage detection

  • Pan, Chu-Dong;Yu, Ling;Chen, Ze-Peng;Luo, Wen-Feng;Liu, Huan-Lin
    • Smart Structures and Systems
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    • v.17 no.6
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    • pp.957-980
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    • 2016
  • Structural damage detection (SDD) is a challenging task in the field of structural health monitoring (SHM). As an exploring attempt to the SDD problem, a hybrid self-adaptive Firefly-Nelder-Mead (SA-FNM) algorithm is proposed for the SDD problem in this study. First of all, the basic principle of firefly algorithm (FA) is introduced. The Nelder-Mead (NM) algorithm is incorporated into FA for improving the local searching ability. A new strategy for exchanging the information in the firefly group is introduced into the SA-FNM for reducing the computation cost. A random walk strategy for the best firefly and a self-adaptive control strategy of three key parameters, such as light absorption, randomization parameter and critical distance, are proposed for preferably balancing the exploitation and exploration ability of the SA-FNM. The computing performance of the SA-FNM is evaluated and compared with the basic FA by three benchmark functions. Secondly, the SDD problem is mathematically converted into a constrained optimization problem, which is then hopefully solved by the SA-FNM algorithm. A multi-step method is proposed for finding the minimum fitness with a big probability. In order to assess the accuracy and the feasibility of the proposed method, a two-storey rigid frame structure without considering the finite element model (FEM) error and a steel beam with considering the model error are taken examples for numerical simulations. Finally, a series of experimental studies on damage detection of a steel beam with four damage patterns are performed in laboratory. The illustrated results show that the proposed method can accurately identify the structural damage. Some valuable conclusions are made and related issues are discussed as well.

Mechanism on the Hydrolysis of Cinnamonitrile in Strong Acid (强酸性 溶液中에서 Cinnamonitrile의 加水分解 反應메카니즘)

  • Ki-Sung Kwon;Nack-Do Sung;Tae-Rin Kim;Jeon, Yong Gu
    • Journal of the Korean Chemical Society
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    • v.28 no.6
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    • pp.418-424
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    • 1984
  • Rate constants for the hydrolysis of cinnamonitrile in the concentration range of 1 ∼ 5M of perchloric acid at 25$^{\circ}$C have been determined by UV spectrophotometry and from the Bunnett equations, hydration parameters (${\omega}$ = 9.8, ${\omega}^*$ = 0.42 & ${\phi}$=1.6) were obtained. CNDO/2 MO calculations were performed to determine relative stability, net charges, and overlap population of various conformational isomers. The results show that the (E)-planar is more stable than the (Z)-planar and protonation is favored on the nitrogen atom. On the basis of above findings, the acid hydrolysis is initiated by the protonation of the nitrogen atom of cinnamonitrile and then water molecule acting as nucleophile and as a proton transfer agent in the rate determining step. In the transition state of the acid hydrolysis, nucleophilic addition of water molecule occurs by sigma approach to the positively charged $C_7({\alpha}$) atom of the conjugate acid. As the results, we may conclude that the hydrolysis of cinnamonitrile in the strong acidic media proceeds through the A-2 type mechanism.

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Characteristic analysis of the current type high frequency resonant DC - DC converter (전류형 고주파 공진 DC-DC 컨버터의 특성해석)

  • 황계호;남승식;김동희;심광열;안항목
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.17 no.1
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    • pp.86-93
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    • 2003
  • This paper describes that the resonant tank type DC-DC converter consist of reactor and capacitor resonant tank circuit for increased the output current. This circuit configuration is composed of the resonant tank circuit used resonant capacitor and reactor and the capacitor connected in switch are a common using by resonance capacitor and ZVS(Zero Voltage Switching) capacitor. Therefore, the proposed converter can reduce a switching losses, noise, and voltage stress at turn-on and turn-on and has an advantage which is able to operating safely in load short, because DC reactor is connected with resonance reactor in order to supply a fixed current with low ripple from DC power supply. The analysis of proposed circuit uses normalized parameters and characteristic estimation is generally described the proposed circuit with the characteristics of power and output voltage etc. Also, design is based on the characteristic estmations in each step. Hence, We conform a rightfulness theoretical analysis by comparing a theoretical values and experimental values obtained from experiment.