• Title/Summary/Keyword: Stem number

Search Result 1,367, Processing Time 0.029 seconds

The use of pituitary adenylate cyclase-activating polypeptide in the pre-maturation system improves in vitro developmental competence from small follicles of porcine oocytes

  • Park, Kyu-Mi;Kim, Kyu-Jun;Jin, Minghui;Han, Yongquan;So, Kyoung-Ha;Hyun, Sang-Hwan
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.32 no.12
    • /
    • pp.1844-1853
    • /
    • 2019
  • Objective: We investigated how pituitary adenylate cyclase-activating polypeptide (PACAP) affects embryonic development during pre-in vitro maturation (pre-IVM) using porcine oocytes isolated from small follicles. Methods: We divided the follicles into the experimental groups by size (SF, small follicles; MF, medium follicles) and treated with and without PACAP and cultured for 18 hours (PreSF[-]PACAP; without PACAP, Pre-SF[+]PACAP; with PACAP) before undergoing IVM. The gene expression related to extracellular matrix formation (amphiregulin, epiregulin, and hyaluronan synthase 2 [HAS2]) and apoptosis (Bcl-2-associated X [BAX], B-cell lymphoma 2, and cysteine-aspartic acid protease 3) was investigated after maturation. The impact on developmental competence was assessed by the cleavage and blastocyst rate and total cell number of blastocysts in embryos generated from parthenogenesis (PA) and in vitro fertilization (IVF). Results: Cleavage rates in the Pre-SF(+)PACAP after PA were significantly higher than SF and Pre-SF(-)PACAP (p<0.05). The cleavage rates between MF and Pre- SF(+)PACAP groups yielded no notable differences after IVF. Pre-SF(+)PACAP displayed the higher rate of blastocyst formation and greater total cell number than SF and Pre-SF(-)PACAP (p<0.05). Cumulus cells showed significant upregulation of HAS2 mRNA in the Pre-SF(+)PACAP compared to the SF (p<0.05). In comparison to other groups, the Pre-SF(+)PACAP group displayed a downregulation in mRNA expression of BAX in matured oocytes (p<0.05). Conclusion: The PACAP treatment during pre-IVM improved the developmental potential of porcine oocytes derived from SF by regulating cumulus expansion and apoptosis of oocytes.

The effect of water status on productive and flowering variables in young 'Arbequina' olive trees under limited irrigation water availability in a semiarid region of Chile

  • Beya-Marshall, Victor;Herrera, Julio;Fichet, Thomas;Trentacoste, Eduardo R.;Kremer, Cristian
    • Horticulture, Environment, and Biotechnology : HEB
    • /
    • v.59 no.6
    • /
    • pp.815-826
    • /
    • 2018
  • The intense drought affecting olive production in Northern Chile underscores the need to research non-traditional irrigation strategies to obtain the best crop performance. Accordingly, this study aimed to obtain preliminary data to guide future research on this topic. Different water replenishment levels on crop evapotranspiration ($ET_c$ ; 13.5, 27.0, 40.5, and 54%) were established in a young orchard, cv. Arbequina, from the end of fruit drop (EFD) to full bloom in the next season. We evaluated the influence of plant water status (${\Psi}_{stem}$ ) and crop load, considered as function of fruit number divided by trunk cross-sectional area, on reproductive and productive variables using multiple linear regressions. Our results show that crop load and ${\Psi}_{stem}$ measured from EFD to harvest affected yield components. Nevertheless, ${\Psi}_{stem}$ had the strongest influence on fruit size, pulp development, oil accumulation, and yield. Oil content and yield were reduced by 54% and 50% for each MPa, respectively, from ${\Psi}_{stem\;EFD-H}$ -1.8 MPa, an effect that intensified as crop load increased. During the period of flower development (September-November), the number of flowers per inflorescence and percentage of perfect flowers were reduced when ${\Psi}_{stem}$ was less than -2.0 MPa. These preliminary results showed that bud differentiation, inflorescence and flower formation are highly sensitive to water deficit.

Retrieval of Porcine Ovarian Follicles by Different Methods

  • Choi, Moon Hwan;Gong, Seung Pyo;Lim, Jeong Mook
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.21 no.3
    • /
    • pp.353-357
    • /
    • 2008
  • A series of studies has been conducted to establish a base infrastructure for an ovarian follicle culture system in the porcine and this study was designed to develop an effective retrieval protocol of preantral follicles. Five different methods using collagenase type I (A) or IV (B, C1, C2 and C3), which employed different treatment durations and/or conditions, were employed and sliced ovarian tissue of prepubertal gilts was provided for the retrieval. A significant increase in total number of follicles retrieved was detected when collagenase IV (methods B or C) was used. In total, more ovarian follicles were retrieved by method B undertaking agitation and method C2 without the agitation than method C1 and C3, while the number of preantral follicles collected was the largest in method B. Neither incubation in 5% $CO_2$ in air atmosphere instead of the agitation nor increased duration of enzymatic treatment up to 120 minutes improved the efficiency of follicle retrieval. There were no differences in the number of follicles retrieved from intact ovaries and from used ovaries for oocyte collection. These results demonstrate the collagenase IV treatment with agitation is effective for retrieving porcine preantral follicles from the ovaries.

Distribution, Size and Development Phases of Knots for Pinus sylvestris L. var. mongolica Litvin in Northeast China

  • Jia, Weiwei;Li, Fengri
    • Journal of Korean Society of Forest Science
    • /
    • v.94 no.5 s.162
    • /
    • pp.313-320
    • /
    • 2005
  • This study was performed in a 38-year-old Mongolian pine (Pinus sylvestris L. var. mongolica Litvin) plantation in northeast China. Data were collected from 5 sample trees with different canopy position ranging in DBH from 14.6 cm to 23.8 cm. Sawn speciments that included the biggest knot were taken from the stem below the living crown. Number and distribution of knots per whorl below the living crown were studied by relative height below living crown (RHBC). A linear model expressed as function of whorl age (AGE), whorl height ($H_k$) and the stem diameter at which the whorl was located ($D_k$) was developed to predict the knot diameter and angle. The number of annual rings in four periods and the width of respective zone alone stem were used as dependant variables to analyze the knot develop phases. In average, the number of years from branch birth to ceased forming rings was 7.8, the branches remained alive for 4.2 years without forming annual rings, and branches were occluded 14.4 years after their death. These results can provide abundance branch and knot information so as to describe current and past tree growth dynamic of Mongolian pine plantation.

Interpretation of Relationship Between Sesame Yield and It's components under Early Sowing Cropping Condition

  • Shim Kang-Bo;Kang Churl-Whan;Seong Jae-Duck;Hwang Chung-Dong;Suh Duck-Yong
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.51 no.4
    • /
    • pp.269-273
    • /
    • 2006
  • Multiple linear regression analysis was conducted to interpretate the relationship between sesame grain yield and its components under early sowing cropping condition. The t test showed that stem length, number of capsules per plant, 1000 seeds weight and seed weight per plant gave significant contribution to sesame grain yield, therefore those variables were assumed to mostly influenced components to grain yield of sesame. In the stepwise regression analysis, the predicted equation for sesame grain yield per square meter (Y) was Y = -7.900 + 0.150X1 + 0.461X5 + 15.553X6 + 8.543X7. Meanwhile, F value showed that stem length, number of capsules per plant and seed weight per plant gave significant contribution to sesame grain yield, while 1000 seeds weight did not significantly show. Based on the results, it is reasonable to assume that high yield. potential of sesame under early sowing cropping condition would be obtained by selecting breeding lines with long stem length, number of capsules per plant, and seed weight per plant, which was different result at the late sowing cropping condition in which days to flowering and maturity were assumed to be more affected factors to the sesame grain yield.

Estimation of the Potato Growth Information Using Multi-Spectral Image Sensor (멀티 스펙트럴 이미지 센서를 이용한 감자의 생육정보 예측)

  • Kang, Tae-Hwann;Noguchi, Noboru
    • Journal of Biosystems Engineering
    • /
    • v.36 no.3
    • /
    • pp.180-186
    • /
    • 2011
  • The objective of this research was to establish the estimation method of growth information on potato using Multi-Spectral Image Sensor (MSIS) and Global Positioning System (GPS). And growth estimation map for determining a prescription map over the entire field was generated. To determine the growth model, 10 ground-truth points of areas of $4m^2$ each were selected and investigated. The growth information included stem number, crop height and SPAD value. In addition, images information involving the ground-truth points were also taken by an unmanned helicopter, and reflectance value of Green, Red, and NIR bands were calculated with image processing. Then, growth status of potato was modeled by multi-regression analysis using these reflectance value of Green, Red, and NIR. As a result, potato growth information could be detected by analyzing Green, Red, and NIR images. Stem number, crop height and SPAD value could be estimated with $R^2$ values of 0.600, 0.657 and 0.747 respectively. The generated GIS map would describe variability of the potato growth in a whole field.

The Effect of Electroacupuncture on Pain Threshold and 5.Hydroxytryptamine Synthesis Induced by Nociception in the Raphe Magnus Nucleus of Dog Brain Stem (개의 발에 염증 유발 후 전침이 통증역치 및 뇌줄기 큰솔기핵의 5-Hydroxytryptamine 합성에 미치는 효과)

  • Cho, Mi-Suk
    • The Journal of Korean Physical Therapy
    • /
    • v.23 no.1
    • /
    • pp.59-66
    • /
    • 2011
  • Purpose: The purpose of this study was to investigate the effect of electroacupuncture on pain threshold and 5-HT synthesis in raphe magnus nucleus of dog brain stem following nociception induced by 1% carageenan. Method: Twenty male dogs were randomly divided into two groups, a control group and an electroacupuncture group with the following parameters: ST36, LI11 and SP9 with 120Hz and 0.5mA. The dogs were sacrificed at 30, 60, 90, 120, 150 and 180 min after nociception using 1% carageenan injection into the plantar side of the dog's left leg. The pain threshold was recorded by an Analgesia-meter. After making brain slice sections, the tissues were immunostained with 5-HT antisera (1:2,500). Results: The pain threshold for the electroacupuncture group was higher than for the control group. The number of 5-HT immunoreactive neurons in the electroacupuncture group was greater than that of the control group. Differences between the two groups regarding the number of 5-HT immunoreactive neurons at 30 min and 60 min (p<0.05), at 120 min and 150 min (p<0.01), and at 8 days (p<0.05) were significant. Conclusion: The increases in pain threshold and number of 5-HT immunoreactive neurons in the raphe magnus nucleus of dog brain stem showed that electroacupuncture can attenuate pain transduction through the descending tract.

Studies on Dry Matter Production and Variation of Agronomic Characteristics of Determinate and Indeterminate Types of Soybean Cultivars (Glycine max L.) Under Different Growing Condition (유ㆍ무한형대두품종의 재배조건에 따른 건물생산 및 형질변이에 관한 연구)

  • Keun-Yong Park
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.17
    • /
    • pp.45-78
    • /
    • 1974
  • To provide useful information for developing new high yielding soybean varieties and for improving cultural practices, an investigation was made on variation of dry matter production and on relationship among several agronomic characters of soybean plants grown under different planting times and densities as well as under different fertilizer levels, using Kwang-kyo, Dong puk-tae, and Suke # 51 as determinate types and Shelby, SRF-300 and Harosoy as indeterminate types at the Crop Experiment Station during the period of 1972 and 1973. The results obtained were summarized as follow: 1. The dry weight, CGR and LAI at the initial flowering stage were high in the high plant population irrespective of varieties, planting times, and fertilizer levels. However, those characters of the indeterminate type were lower than those of the determinate types. The same characters of the indererminate type at the terminal leaf stage were either same or higher than those of the determinate types. 2. The dry weight of the determinate type at the initial flowering stage was similar to the indeterminate, type, when planting times were May 21 or June 15. The dry weights of both types of varieties were low when planted on July 10. When fertilizer levels were increased, the CGR, dry weight and LAI at the initial flowering stages were also increased. 3. Even though significant differences of LAI were obtained among the varieties within the same plant type, the indeterminate type was in general lower than that of the determinate type regardless of planting time and densities, or fertilizer levels, while the yield of the indeterminate type was comparable to the yield of the determinate type. 4. The high degree of leaf- and petiole-fall at the greenbean stage was highly associated with early planting and high levels of fertilizers. However, less amount of leaf- or petiole-fall was found when planted on July 10 or under low plant population. 5. The percent of stem weight was high under higher plant population, while the percent of leaf weight was high under lower plant population. When planting time was late, the percent of stem and petiole weight were reduced, while the leaf weight was increased. 6. The percent of pod weight of the determinate type at the terminal leaf stage was about 2% when planted on May 21, about 8% when planted on June 15, and about 9% when planted on July 10. The percent of pod weight of the indeterminate type at the terminal leaf stage were about 6 % when planted on May 21, 14% when planted on June 15 and 21% when planted on July 10. 7. Kwang kyo showed less degree of leaf-fall even when lodged due to high levels of fertilizer applied, while SRF-300 showed great damage due to lodging. 8. High yields were obtained when planted on May 21, but there were little yield differences between yields from May 21 and June 15 plantings. The reduction of yield due to late planting of July 10 was less apparent in the determinate type of varieties, while it was high in the indeterminate type. 9. The optimum plant population per are for high yield was 1, 250 to 2, 500 plants when planted on May 21, 2, 500 plants when planted on June 15, and 3, 333 plants when planted on July 10. 10. High correlation coefficients were obtained between dry matter weight and LAI at the terminal leaf stages, and between the dry matter weight and yield at the greenbean stages. The optimum dry weight for high yield in the determinate type was expected to be 25 kg. per are at the initial flowering stage and 50 kg. per are at the terminal leaf stage. In the indeterminate type the LAI and dry weight at the greenbean stage were 4 to 5 and 80 kg. per are, respectively. 11. Under the high plant population plant height was increased, while the stem diameter and the number of nodes and branches were reduced. Consequently, the percent of mainstem to main stem plus branches were increased, and the length of internode was also elongated. The ratios of stem weight, number of nodes and pods, and yield of main stem were increased when high plant population was associated with the early planting. The percent of main stem to branches for the indeterminate type was higher than that of the determinate type. 12. Under the high plant densities and late planting, the percent of the pod number and yields of main stem were increased, indicating that varieties with no or less branches were better adaptable under such conditions. 13. High degree of simple correlation coefficients was obtained between the LAI at the initial flowering stage and terminal leaf stage, and the total node number, dry matter and dry stem weight of both determinate and indeterminate types. Even though no significant correlation was found between the LAI at the initial flowering stage of the determinate type and the stem length and pod number per are, highly significant correlation coefficients were obtained between such characters in the indeterminate type of varieties. 14. The dry matter was positively correlated with the LAI, CGR, stem length, and pod number, node number and dry stem weight per are, while no significant correlation was found between the dry matter and stem diameter. 15. The correlation coefficients between lodging index and the LAI, dry weight, stem length and dry stem weight were highly significant. Negative correlation was obtained for the indeterminate type between the stem diameter and lodging index. The correlation coefficient between the stem diameter and lodging index was non-significant for the determinate type, while positive correlation was obtained between the yield and lodging index in the determinate type. The lodging index was also positively correlated with average length of internode of main stem. 16. The 100 seed weight appeared to be lowered under the high plant population and no fertilizer condition, and when planted late. Apparent differences of 100 seed weight were found between main stem and branches, being higher for the main stem than for the branches. 17. No variation of protein content was found due to different cultural practices. However, the oil content was apparently reduced when planted late.

  • PDF

Effect of Stem Number on Growth, Fruit Quality, and Yield of Sweet Peppers Grown in Greenhouses under Supplemental Lighting with High Pressure Sodium Lamps in Winter (겨울철 고압나트륨등 보광 하에서 온실재배 파프리카의 줄기 유인 수가 생육, 과실 품질 및 생산량에 미치는 영향)

  • Yoon, Seungri;Kim, Jin Hyun;Hwang, Inha;Kim, Dongpil;Shin, Jiyong;Son, Jung Eek
    • Journal of Bio-Environment Control
    • /
    • v.30 no.3
    • /
    • pp.237-243
    • /
    • 2021
  • The objective of this study was to evaluate the effect of stem number on plant growth, fruit quality, and yield of sweet peppers grown in greenhouses under supplemental lighting in winter. The seedlings were transplanted at 3.2 plants·m-2 on October 26, 2020, and started supplemental lighting with 32 high pressure sodium lamps for 16-hour photoperiod from December 1, 2020 to May 25, 2021. Stems were differently trained with 2 and 3 numbers after branching nodes were developed. In the final harvest, the plant height was significantly shorter in the 3 stem-plants than in the 2 stem-plants. The number of nodes per stem and the leaves per plant were increased in the 3 stem-plants than in the 2 stem-plants, while the leaf area was less affected. There were no significant differences in the dry mass of leaves, stems, and immature fruits between the 2 and 3 stem-plants. The fruit fresh weight and fruit dry weight in the 3 stem-plants were decreased by 17% and 12% at 156 days after transplanting (DAT), and by 17% and 15% at 198 DAT compared to those in the 2 stem-plants, respectively. The marketable fruit rates were 93.6% and 95.4% in the 2 and 3 stem-plants, respectively. The total fruit yield in the 3 stem-plants was increased by 30.2% as compared to that in the 2 stem-plants. We concluded that the 3-stem-training cultivation positively affected the total fruit yield by sustaining adaptive vegetative growth of the plants. This result will help producers make useful decisions for increasing productivity of sweet peppers in greenhouses.

Comparison of Neural Cell Differentiation of Human Adipose Mesenchymal Stem Cells Derived from Young and Old Ages (연령별 지방 중간엽 유래 줄기세포의 신경세포로의 분화 능력 비교)

  • Jo, Jung-Youn;Kang, Sung-Keun;Choi, In-Su;Ra, Jeong-Chan
    • Development and Reproduction
    • /
    • v.13 no.4
    • /
    • pp.227-237
    • /
    • 2009
  • Recently, adipose mesenchymal stem cells (AdMSC) that are similar to bone marrow MSC and blood derived MSC are thought to be another source for stem cell therapy. However, the diseases that can be applied for stem cells therapy are age-dependent degenerative diseases. Accordingly, the present study investigated the growth and differentiation potential to neural cells of human AdMSC (hAdMSC) obtained from aged thirty, forty and fifty. The growth of cells and cell viability were measured by passage and neural differentiation of hAdMSC was induced in neural differentiation condition for 10 days. Our results demonstrated that cell number, viability and morphology were not different from hAdMSC by age and passage. Immunofluorescence analysis of neural cell marker (TuJ1, NSE, Sox2, GFAP or MAP2) demonstrated no significant differences in neural cell differentiation by age and passage. As the number of passage was increased, the mRNA level of MAP2 and Sox2 was decreased in hAdMSC from age of 50 compared to hAdMSC from age of 30. In conclusion, the present study demonstrated that ability of neural cell differentiation of hAdMSC was maintained with ages, suggesting that autologous stem cells from aged people can be applied for stem cell therapy with age-dependent neural disease with the same stem cell quality and ability as stem cell derived from young age.

  • PDF