• Plant Biotechnology Reports
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• v.3 no.3
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• pp.243-250
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• 2009

In the present study, a simple one medium formulation protocol for callus culture, somatic embryogenesis and in vitro production of ${\beta}-carboline$ alkaloids and diosgenin in Tribulus terrestris L. was developed. Extensive callus induction and proliferation was obtained in leaf explant on Murashige and Skoog (MS) medium supplemented with $5.0{\mu}M$ 6 benzyl adenine (BA) and $2.5{\mu}M$ ${\alpha}-naphthaleneacetic$ acid (NAA). The embryogenic callus was maintained on subculture to fresh parental medium at 4-week intervals over a period of 28 months. The frequency of embryo formation was at a maximum ($18.1{\pm}0.9$ per g of callus) on MS medium containing $5.0{\mu}M$ BA and $2.5{\mu}M$ NAA together with $75mg\;1^{-1}$ casein hydrolysate. Globular embryo developed into torpedo stage embryo under the influence of starvation. The accumulation of ${\beta}-carboline$ alkaloids (harmaline and harmine) and steroidal saponin (diosgenin) in non-embryogenic and embryogenic callus culture derived from leaf explant was compared with root, leaf, stem, and fruit of the mother plant. The embryogenic callus accumulated equivalent amounts of harmaline ($66.4{\pm}0.5{\mu}g/g$ dry weight), harmine ($82.7{\pm}0.6{\mu}g/g$ dry weight), and diosgenin ($170.7{\pm}1.0{\mu}g/g$ dry weight) to that of the fruit of T. terrestris. The embryogenic callus culture of this species might offer a potential source for production of important pharmaceuticals.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.35 no.4
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• pp.328-333
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• 1990

In developing dynamic growth model of a crop, it is important to estimate accurate dry matter partition to different parts of crop plants. Two rice varieties, Samkang and Chucheong, were transnplanted with three planting densities of 72. 90 and 120 hills per 3.3㎡ on May 30 and June 15 in 1988 to study the effect of planting density on dry matter partition in rice plants. Total dry wight per square meter of two varieteis in May 30 transplanting were greater than those in June 15 transplanting. Total dry wights were increased as planting density was increased. The response of dry weights of differents parts of rice plants per hill were decreased as the density was increased. Although the difference in dry weights of leaf blade and stem and sheath between two varieties was not great, greater ear weight of Samkang resulted in greater total dry weight than that of Chucheong. Despite of transplant in date and planting density on dry weights, the ratio of dry matter partition to different parts of rice plants at a certain growth stage remained constant. Estimated dry weights of different parts at two stages of growth based on average ratio of dry matter partition over two transplantion dates and planting densities agreed well with those observed.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.5
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• pp.431-436
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• 1994

The objective of experiment was to investigate the effects of sowing dates on forage yield and growth characteristics of rye in paddy field cultivation in midwest region of Korea. The field experiment treated with 5 levels of sowing dates was carried out at Yesan from october 1990 to june 1991. The field emergence rate and winter survival rate were decreased with delay in sowing dates by the days from sowing to emergence were took a long period of time. The crop growth rate were increased with early in sowing date. The fresh and dry weight of rye were increased with early in sowing date, but there was no significant differences between sowing date from Oct. 5 to Oct. 25. The highest fresh yield was obtained at flowering stage and the highest dry yield was obtained at milky stage. The ratio of leaf blade and leaf sheath in rye plant were decreased with earlier sowing date and delayed harvest, but the ratio of stem and inflorescence was vice versa.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.6
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• pp.356-360
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• 2000

With isogenic waxy and non-waxy rice cultivars, growth responce as affected by leaf-removal treatment were examined in various fertilizer application. Vertical dry matter of culm was significantly decreased from ground upto 10cm. Culm dry matter of waxy rice was more steadily decreased than that of non-waxy rice. Starch content of culm in non-waxy rice was higher than waxy rice, and that in waxy and non-waxy rice were the lowest in three leaf-removal but no difference in single leaf-removal. Flag leaf in waxy rice and 2 nd leaf in non-waxy rice were dominantly affected yield by leaf-removal. The 1000-grain weight and rippend grain ratio of non-waxy rice were more higher than waxy rice and that in waxy and non-waxy rice were the lowest value in three leaf-removal but no difference in single leaf-removal treatments.

• Korean Journal of Agricultural Science
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• v.14 no.2
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• pp.232-247
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• 1987

Three rice varietis, Dongjinbyeo, Daecheongbyeo and Chucheongbyeo, which are leading cultivars in the Chungnam area, were planted to determine their effects of mixture on the plant characteristics and the blast occurrence. The summary obtained is as follows; 1. The heading dates of each cultivars were not affected by the mixing culture. Only a day was delayed in heading date of Chucheongbyeo under commonly standard fertilizer level. 2. The effects of cultivar mixture on the plant height was high when low level of fertilizer were applied. Of the cultivar mixture, the mixing of Dongjinbyeo and Daecheongbyeo shoed 5.1% of plant height increase. The effects of mixture on the tillering number were apparent only under the standard fertilizer level. The mixture of Dongjinbyeo and Chucheongbyeo was effective in increasing the tillering number up to 5.7% over mean tillering number of each cultivars. 3. The stem height tended to increase as the fertilizer level increased and the effects of cultivar mixture on the stem height was high. The stem height of mixture of Dongjinbyeo and Daecheongbyeo was highest compared with other mixture cropping. On the contrary, the panicle length was shortened as the fertilizer level decreased and cultivars were mixed. Among the cultivar mixtures, the mixture of Dongjinbyeo and Daecheongbyeo showed the most effectives of increasing the panicle length. 4. The total dry weight of mixed and pure cultivars were compared. The mixture of two cultivars was effective in increasing the dry weight of rice upto 3.5% over the average of dry weight of each cultivars. When three cultivars were mixed, there were 8.5% of dry weight increase over not mixed cultivars. The effects of mixture on the dry weight were more apparent under standard fertilizer level. 5. The rates of Neck and Node and Branch Blast occurrence were reduced when cultivars were mixed, and their reduction rates were 11.7% and 14.0%, respectively. The occurrence of Blasts was also decreased under the lowest fertilizer level than the standard fertilizer level. The least Blast occurrence was obtained when Dongjinbyeo and Daecheongbyeo were mixed cropped. 6. Significantly high number of spikelets of per square meter was observed when Chucheongbyeo and Dongjinbyeo were mixed. However, the spikelets of panicle were the lowest when Chucheongbyeo and Dongjinbyeo were mixed. The number of panicles per square meter was increased as the fertilizer levels increased, while the number of spikelets per panicle increased as the fertilizer level decreased. 7. The effects of mixture on the maturing were apparent regardless of the fertilizer level. The 1000 grain weight was higher when lower level of fertilizer was applied. The effects of cultivar mixture on the 1000 grain weight were more apparent in the plots of Chucheongbyeo and Dongjinbyeo mixture and three cultivar mixture. 8. The grain yield increased when cultivars mixed. The increment under mixing cropping was 4.6% over mono cropping. The effects of cultivar mixture on the yield increase were more apparent under lower levels of fertilizer application. The highest yield increase was obtained when Chucheongbyeo and Daecheongbyeo were mixed, and the rate of yield increase was 6.8% over mono-cropping. 9. The grain yield was highly correlated with number of panicles per square meter and dry weight. There was not any significant relationships found among grain yield, spikelets of panicle and ripening percentage.

• Journal of Korea Foresty Energy
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• v.7 no.1
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• pp.1-10
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• 1987

This study was carried out to estimate the aboveground biomass of Cryptomeria japonica and Chamaecyparis obtusa which was planted in Changsong district, the southern part of the Korean peninsula. Nine sample trees at each plot(20m x 20m drom C. japonica of 20-year-old and C. obtusa of 25-year-old, according to DBH distribution. were felled to measure the dry weights of stem, branches and leaves sectioned respectively. Since it is very difficult to separate leaves from twigs. all green parts including pure leaves and twigs were tentatively treated as 'leaves'. 1. The logarithmic regression equations between dry weight of each component (stem. branches. leaves land the variable of $(DBH)^2{\cdot}H$ were obtained (Table 6). The aboveground standing crops was estimated 108. 75ton/ha in C. japonica and 112.56 ton/ha in C. obtusa. Percentage of each part based on the aboveground standing crop in stand was and in order of stem >leaves>branches for C. japonica and in order of stem> branches> leaves for C. obtusa repectively. 2. Net production of each stand was estimated as 1:3.32ton/ha/yr in C. japonica and 11.69ton/ha/yr in C. obtusa. and its composition was in order of stem>leaves>branches for both species. 3The net assimilation rate was estimated as 1.10 and 1.2lkg/kg/yr, the efficiency of leaves to produce stem was 0.71 and 0.75 kg/kg/ha and biomass accumulation ratio was 8.16 and 9.63kg/kg/yr in each stand of C. japonica and C. obtusa respectively.

• Journal of Korean Society of Forest Science
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• v.97 no.5
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• pp.508-515
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• 2008

Sycamore (Platanus occidentalis L.) seedlings were grown under low light intensity and ozone treatments to investigate the role of the light environment in their response to chronic ozone stress. One-year-old seedlings of Platanus occidentalis L. were grown in pots for 3 weeks under low light (OL, $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) and high light (OH, $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) irradiance in combination with 150 ppb of ozone fumigation. After three weeks of ozone and light treatment, seedlings were placed in ozone free clean chamber for 3 weeks for recovery from ozone stress with same light conditions to compare recovery capacity. Ozone fumigation determined an impairment of the photosynthetic process. Reduction of leaf dry weight (14%) and shoo/root ratio (17%) were observed in OH treatment. OL treatment also showed severe reductions in leaf dry weight and shoot/root ratio by 48% and 36% comparing to control, respectively. At the recovery phase, OH-treated plants recovered their biomass, whereas OL-treated plant showed reduction in leaf dry weight (52%) and shoot/root ratio (49%). OH-treated plants reached similar relative growth rate (RGR) comparing to control, whereas OL-treated plants showed lower RGR in stem height. However, there were no significant differences in response to those treatments in stem diameter RGR at the recovery phase. Ozone treatment produced significant reduction of net photosynthesis in both high and low light treatments. Carboxylation efficiency and apparent quantum yield in OL-treated plants showed significant reductions rate to 10% and 45%, respectively. At the recovery stage, ozone exposed seedlings under high light had similar photosynthetic capacity comparing to control plants. Antioxidant enzymes activities such as superoxide dismutase (SOD), ascorbate peroxidase (APX), and glutathione reductase (GR) were increased in ozone fumigated plants only under low light. The present work shows that the physiological changes occur in photosynthesis-related parameters and growth due to ozone and low light stress. Thus, low light seems to enhance the detrimental effects of ozone on growth, photosynthesis, and antioxidant enzyme responses.

• Journal of The Korean Society of Grassland and Forage Science
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• v.4 no.3
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• pp.187-193
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• 1984

Using the stratifying clip method, we examine the differences in vertical distribution of leaf area and its relationships with dry matter production were investigated in the primary swards of six varieties. The results obtained were as follows: 1. The varieties could be classified into 4 different types according to their of vertical distribution of leaf area within the canopy. Thus, M had a greater leaf area distribution in the base layers and became smaller towards the upper layers, Leto, First and Bundy had the leaf area distribution which was most abundant in the middle layers and became smaller towards the base and the upper layers, Tammisto had a greater leaf area distribution in the middle layers and intensively distributed to the upper layers, and Trader had the relatively uniform leaf area distribution over all layers. 2. Effective leaf area index(ELAI) correlated with leaf area index(LAI), dry weight of plant(DW), stem weight of plant(SW) and stem area index(SAI). 3. The variety with the heading tiller type had the effective leaf area intensively distributed to the upper layers of canopy, but the vegetative type showed a high ratio of effective leaf area index. 4. Varietal differenced was found between the effective leaf area and specific leaf weight(SLW) in each stratum above the layer indicating the highest leaf area density within the canopy. M and Trader had a highly negative significant correlation, but the other varieties was not significant correlation.

• Korean Journal of Agricultural Science
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• v.11 no.1
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• pp.77-84
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• 1984

This experiment was carried out to determine the effects of night temperature and amount of fertilization on growth and photosynthesis of red pepper plants for providing some basic imformation needed in improving the productivity of red pepper. 1. Plant height, stem length, number of flower buds, dry weight of plants and photosynthetic rate were higher in King Gun Gochu than in Dohusa, but number of leaves, number of internodes and leaf area we re higher in Dohusa compared to King Gun Gochu. 2. Plant height, number of leaves, number of flower buds, dry weight of plants, leaf area and photosynthetic rate were significantly increased by 50% increased fertilization compared to normal fertilization. 3. High night temperature($25^{\circ}C$) treatment increased plant height, stem length, number of leaves, number of internodes, number of flower buds, dry weight of plants and leaf area. 4. RGR and LAR were increased by 50% increased fertilization and high night temperature but NAR was decreased by high flower buds 5. Photosynthetic rate of King Gun Gochu was increased by 50% increased fertilization and high light intensity. 6. Number of flower buds was increased at King Gun Gochu by 50% increased fertilization and high night temperature ($25^{\circ}C$).

• Journal of the Korean Society of Tobacco Science
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• v.7 no.1
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• pp.15-23
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• 1985

Growth response and evapotranspiration of tobacco as to each soil moisture condition treated with different height of ground water table such as 30cm, 100cm, 170cm, and 230cm were investigated. The results obtained were as follows; 1. Soil water potential in the rhizosphere (25cm depth from surface) treated with ground water table (G.W.T) of 230 cm was less than -6.5 bar at 60 days after transplanting. 2. Dry weight of leaves per plant was the highest in the pot treated with 100 cm G.W.T and was the lowest in the pot treated with 230 cm G.W.T.. Leaves/Stem ratio of dry weight was the lowest in the pot treated with 230 cm G.W.T. This would show that extension of leaves as compared with elongation of stem was suppressed. .3. In the roster stress condition of 230 cm G.W.T., extension of leaves was continued until the latter growth stage, Relative growth rate of leaf area per plant of the pot with 30 cm G.W.T. was the highest through all growth period after fixation of G.W.7., but owing to lower density of leaf, the dry weight of leaves per plant was less rather than that of the pot with 170 cm G.W.T. at 64 days after transplanting. 4. The highest evapotranspiration per plant per day was shown at the 45th day after transplanting in the pots with 30cm and 100cm G.W.T. and at 60 days in the pots with 170cm and 230cm G.W.T respectively. 5. In the soil moisture conditions with 30cm and 100cm G.W.T., evapotranspiration per plant per hour was much higher in the daytime that at night as well as in clear day than in rain-cloudy day owing to loner relative humidity , but in the water stress condition with 230 cm G.W.T., that was much lower. 6. Total evapotranspiration per plant during the whole growing period of 64 days was in order of 30cm > 100 cm > 170 cm > 230 cm G.W.T.. Total evapotranspiration for 34 days after fixation of G.W.T was much higher at night than in the daytime in case of water stress conditions i.e 170cm and 230cm G.W.T. but in normal condition of the pots with 30cm and 100cm G.W.T. that was much higher in the daytime than at night. 7. Leaf water potential of the 3rd and 6th leaf from top at 64 days after transplanting was in order of 100cm > 30 cm > 170cm > 230cm G.W.T. The reason why leaf water potential in the pot with 30cm was less than that in the pot with 100cm G.W.T. would be the abnormal turgidity with low density of cell substrates by the excess water absorption in the pot with 30cm G.W.T..