• Maxillofacial Plastic and Reconstructive Surgery
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• v.33 no.5
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• pp.385-391
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• 2011

Purpose: The periosteum is a well-known source of osteogenic precursor cells for tissue-engineered bone formation. However, cultured endothelial or endothelial-like cells derived from periosteum have not yet been investigated. This study focused on endothelial-like cell culture from the periosteum. Methods: Periosteal tissues were harvested from the mandible during surgical extraction of lower impacted third molars. The tissues were treated with 0.075% type I collagenase in phosphate-buffered saline (PBS) for 1 hr at $37^{\circ}C$ to release cellular fractions. The collagenase was inactivated with an equal volume of DMEM/10% fetal bovine serum (FBS) and the infranatant was centrifuged for 10 min at 2,400 rpm. The cellular pellet was filtered through a $100{\mu}m$ nylon cell strainer, and the filtered cells were centrifuged for 10 min at 2,400 rpm. The resuspended cells were plated into T25 flasks and cultured in endothelial cell basal medium (EBM)-2. Results: Among the hematopoietic markers, CD146 was more highly expressed than CD31 and CD34. The periosteal-derived cells also expressed CD90 and CD166, mesenchymal stem cell markers. Considering that the expression of CD146 was constant and that the expression of CD90 was lower at passage 5, respectively, the CD146 positive cells in passage 5 were isolated using the magnetic cell sorting (MACS) system. These CD146 sorted, periosteal-derived cells formed tube-like structures on Matrigel. The uptake of acetylated, low-density lipoprotein, labeled with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI-Ac-LDL) was also examined in these cells. Conclusion: These results suggest that the CD146-sorted positive cells can be referred to as periosteal-derived CD146 positive endothelial-like cells. In particular, when a co-culture system with endothelial and osteoblastic cells in a three-dimensional scaffold is used, the use of periosteum as a single cell source would be strongly beneficial for bone tissue engineering.

• Korean Journal of Agricultural Science
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• v.30 no.1
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• pp.11-19
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• 2003

The life-cycle of the aphid-parasite, Aphidius gifuensis, took 11.9 days at $25^{\circ}C$ and 12.1 days at $30^{\circ}C$. The female appearance rate of A. gifuensis was the best at $20{\sim}25^{\circ}C$ compared to the other temperatures. Optimum rearing temperature of Aphidius gifuensis was $20{\sim}25^{\circ}C$ Optimum temperatures for A. gifuensis emergence were $20^{\circ}C$ and $25^{\circ}C$ with 99.1% and 98.2%, respectively. However the emergence rate of Aphidius gifuensis was 58.8% at $10^{\circ}C$. There was a positive correlation between A. gifuensis adult activity and temperature, but there was a negative correlation between developmental period of Myzus persicae nymph and temperature. Among the pests occurring in pepper greenhouse, dominant species was M. persicae and its density was 6~1,024 per plant. The damaged fruit rate by Helicoverpa assulta was 3.3~53.3%. The number of aphid and mummy in the A. gifuensis released plot were 173.5 and 10 in June, 1.8 and 17 in July, 2000, respectively. The numbers of Aphidius gifuensis were 7.5 and 0.4 in May, 27.1 and 2.1 in June, 2001, respectively. The suppressive effects on M. persicae in A. gifuensis released plot was better than the control plot, but there was no significant difference compared to the pesticide-sprayed plot. The first leaf length and the stem width in the A. gifuensis released plot, the control plot, and the pesticide-sprayed plot were not significantly different, but the A. gifuensis released plot showed the highest yield among the plots.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.102.2-103
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• 2003

This studies were investigated the occurrence of Sclerotinia rot by Sclerotinia Sclerotiorum at the lettuce field in Uiryeong-Gun, Gyeongsangnam-Do and were isolated the most effective microorganism for the biological control to the pathogen, S. sclerotiorum YR-1 strain from diseased soil and lettuce leaves. For the pathogenicity test, the most suitable inoculmn density of YR-1 strain was selected as the mycelial suspension of 40m1 showing disease incidence of 80％, and the symptom showed as same as at the fields, the leaves and stem had rotten and developed white downy mycelial at the diseased lesion on the leaves and stems, and produced black and irregular sclerotinia. On the PDA dual test, about 300 isolates were examined the antifungal activity to the pathogen, YR-1 strain, and among them, A-2, A-7, and RH-4 strain were selected most effective antagonistic bacteria. At pots test, the control value of A-7 strain showed the highest value as 85％ which was more effective than that of others in a growth chamber. For the promotion of control effect, the selected 3 isolates were spayed on the lettuce leaves as a sole and/or mixed treatments in a growth chamber, the mixed treatment of A-7 and RH-4 strain showing the control value of 90％ was most effective than that of sole treatment with A-7 or RH-4 strain showing the control value of 80％, respectively and mixed treatment with A-2 and A-7 strain and A-2, A-7 and RH-4 strain. In addition, 3 bacteria re-isolated from diseased soils, and all of the selected 6 isolates investigated the control effect at pots in a growth chamber, According to the results, A-7 and Pro-EB 15 strain showed the control value of 91.0％ and 90.1％ respectively, and they were selected most effectual antagonistic bacteria to control lettuce sclerotinia rot and identified as the Bacillus mojanuinensis by 16s RNA analysis. This is the first report on the biological control using by B. mojanvinensis to the lettuce Sclerotinia rot.

• Korean Journal of Medicinal Crop Science
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• v.10 no.5
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• pp.344-352
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• 2002

This study was conducted to determine the optimum planting dates and density of one year old Bupleurum falcatum L. to improve its quality. Furthermore, the effect of cultural methods and plant growth regulators$(GA_3,\;IAA,\;Kinetin)$ on the quality of B. falcatum were also investigated. In this study, Jeongseon cultivar collected in Korea and Mishima cultivar introduced from Japan were used. Some of the results obtained are as follows. Jeongseon cultivar showed less stem branches and shoot weight compared to Mishima. However, Jeongseon cultivar showed tall plant height, high root fresh and dry weight, and high levels of SSa and TSS, but low SSc content than that of Mishima. Although fresh and dried root weight of both cultivars were not affected by plant growth regulators treatment. compared with the untreated one, increasement of TSS content were made by $GA_3$ 100 ppm, IAA 50 ppm or 10 ppm and kinetin 10 ppm treatment in Jeongseon cultivar, and by $GA_3$ 10 ppm and IAA 100 ppm or 10ppm treatment in Mishima cultivar applied on June 1.

• Journal of Korean Society of Forest Science
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• v.89 no.4
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• pp.480-487
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• 2000

Repeated measurement of 472 permanent subplots in slash pine (Pinus elliottii Engelm.) plantations were used to develop survival prediction equations for predicting future number of planted slash pine trees. On the average, about 40 percent of the slash pines in the experimental sites had a stem cankers due to fusiform rust (Cronartium quercuum [Berk.] Miyabe ex Shirai f. sp. fusiforme) incidence. A stand level survival prediction model was developed that incorporated the incidence of fusiform rust and allowed the transition paths of trees from an uninfected stage to an infected stage. Predicted total surviving number of trees is obtained by adding together the predicted number of infected and uninfected trees. The influence of natural hardwood density and site quality on slash pine survivals tended to show a negative effects on future survivals.

• Journal of Korean Society of Forest Science
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• v.103 no.3
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• pp.321-338
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• 2014

In the Natural Monument (No. 432) Jeju Sanghyo-dong Cymbidium kanran Habitat (39 ha), flora, vegetation diversity, stand structure, mirco-climate, canopy openness, light environment and soil temperature and moisture were quantified from Oct. 2013 to Feb. 2014. Compare to Seogwipo-si, daily mean temperature ($5.7^{\circ}C$) and moisture (75.8%) in study area were lower at $3.3^{\circ}C$ and 15%, respectively. Mean soil temperature and moisture were $16.5^{\circ}C$ and 37.3%, respectively, and mean litter layer depth (n = 81) was 4.3 cm. Mean canopy openness and light availability at forest floor were 15.5% and $8.5mol{\cdot}m^{-2}{\cdot}day^{-1}$, respectively. Total of 22 species including vascular and bryophyte plants and 6 vegetation group were observed. Castanopsis siebildii was dominant species in study area, and density and basal area were 1,777 stem/ha and $90.3m^2/ha$.

• Journal of Korean Society of Forest Science
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• v.103 no.3
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• pp.375-382
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• 2014

The objective of this study was to investigate suitable plantation site for planted Chamaecyparis obtusa seedlings from the analysis of regional early growth performances. Two years old C. obtusa seedlings were planted with the density of $3,900seedlings{\cdot}ha^{-1}$ in late March, 2011 at Haeman and Jangseong, Jeollanamdo. In each study site, three plots ($400m^2$ per plot) were established and root collar diameter (mm) and tree height (cm) of each C. obtusa were measured in April, 2011 and October from 2011 to 2013. We also analyzed soil physical and chemical properties of sites and compartmental nitrogen and phosphorus concentrations of C. obtusa. Although the concentrations of soil nitrogen, organic matter, and C.E.C. at Haenam site were higher than those at Jangseong site, early growth performances of planted C. obtusa at Jangseong site were significantly better than those at Haenam site. The reasons for these results were probably related to deep available soil depth at Jangseong site and relatively low annual precipitation and sea wind at Haenam site, which was adjacent to the sea. The compartmental nitrogen and phosphorus concentrations of C. obtusa was in the order of needles > current twigs > fine root > stem and branches root above 2 mm in diameter. The phosphorus concentration of needles at Haenam site was significantly higher than that at Jangseong site. The results of this study might be useful for the selection of suitable plantation site for C. obtusa.

• Korean Journal of Environmental Biology
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• v.29 no.4
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• pp.345-352
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• 2011

We investigated 12 plots using Herles' diversity of structure feature calculation method, which is based on the Shannon-Weaver-Index for estimation of species diversity. This study sought to facilitate a more systematic understanding of the structure of the forest stands in the Korean fir natural forest in Naeseorak. Although the change in the forest structure is dependent on the change in phase, factors of the natural forest were confirmed by associating individual structure features. As shown in the results of diversity of structure features, the diversity of the structure of the fir tree natural forest in Naeseorak was relatively low. The association between species diversity and overall factors (diversity) related with the change in the structure was found to be weak. The association between the number of trees and the diversity of forest structure stands was moderate, showing that the higher the number of trees, the less diverse the forest structure is. In most of the investigated plots, stem volume and volume of dead tree were associated with the height of natural regeneration, but these were not associated with other factors. Height of natural regeneration was found to be correlated to stand density, crown area and crown class, whereas tree height and BHD did not have any association with other factors. Overall, the results of the investigation are helpful in understanding the change in the structure of Korean fir natural forest. Further investigation with more plots is required.

• Journal of Forest and Environmental Science
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• v.36 no.3
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• pp.175-186
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• 2020

This study approaches, from a floristic perspective, the under-researched human-primate competition for forest resources. Investigating the human impact on fruit trees edible for Kinda baboons (Papio kindae Lönnberg), we have collated dietary data on a free-ranging troop and floristic information on two forest sites of the Kundelungu National Park (KNP), Democratic Republic of Congo: the relatively intact Integral Zone (IZ) and the human-disturbed Annex Zone (AZ). Trees with DBH≥10 cm have been identified, counted and measured throughout 22 sample plots (11 per site), each measuring 1,000 ㎡. A total of seven woody species whose fruits are eaten by Kinda baboons were recorded. Four of them, namely the Sycamore fig Ficus sycomorus L., the Mobola plum Parinari curatellifolia Planch. ex Benth, the Kudu berry Pseudolachnostylis maprouneifolia Pax and the Monkey orange Strychnos innocua Delile were found in both sites, while the Large-leaved jackal-berry Diosyros kirkii Hiern and the Buffalo thorn Ziziphus mucronata Willd. were exclusively in the IZ, and Strychnos cocculoides Baker only in the AZ. Compared to the IZ, the AZ had lower values of stem density, species richness and diversity indices, suggesting a negative human impact on baboon-edible trees, in line with our hypothesis. Moreover, as was expected, human activities decreased the abundance of larger baboon-edible fruit trees. However, the size-class distribution of P. curatellifolia depicted a reverse J-curve in the AZ. The abundant younger P. curatellifolia trees remaining in that human-disturbed site constitute an important food stock for baboons, if well preserved. These results also illustrate the critical role of rangers' patrols, formerly more frequent (and presumably efficient) in the IZ than in the AZ of the Park. Their implications on baboons and miombo forests are discussed from both the research and conservation perspectives.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.33 no.4
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• pp.293-300
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• 2011

Purpose: Substance P is a well known neurotransmitter and has been known to mediate pain. Recently, it has been unveiled that substance P is involved in the recruitment of mesenchymal stem cells to wound sites. The purpose of this study was to exam bone formation when a combination of substance P and silk fibroin was used in a bone defect model. Methods: Twenty rabbits were used and 40 calvarial defects were formed. They were divided as 4 groups (unfilled control, silk only, silk+$10{\mu}g$/ml substance P; Sub10, and silk+$100{\mu}g$/ml substance P; Sub100). All animals were humanely sacrificed 4 or 8 weeks after grafting. The specimens were analyzed by micro-computerized tomography and histological analysis. Results: When compared to the unfilled control to silk only group, there was significant difference in bone mineral density (BMD) and the attenuation coefficient (AC) at 4 weeks ($p$=0.037 and 0.038, respectively). When compared Sub10 group to Sub100 group, there was significant difference in BMD and AC at 8 weeks ($p$=0.004 for all). Residual graft amounts were $52.1{\pm}15.8$%, $15.2{\pm}9.2$% and $9.0{\pm}3.3$% for silk only, Sub10, and Sub100 groups, respectively. When comparing the residual graft amount of silk only to sub10 or sub100, the differences were statistically significant ($p$ <0.001). Conclusion: The silk fibroin scaffold showed higher BMD and AC than the unfilled control. The combination graft with substance P and silk fibroin scaffold showed a faster graft degradation than with a silk fibroin scaffold only.