• Korean journal of applied entomology
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• v.46 no.1 s.145
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• pp.109-115
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• 2007

This study was carried out to determine occurrence and damage of golden apple snail, Pomacea canaliculata on water seeding area in Jeonnam province. The density of golden apple snail maintained hlgh until October 2003, but quickly decreased from November. They overwintered only in a portion of the water canals but could not find in dried paddy field. They reproduced from May and June in the canal water and paddy field, respectively. Feeding time of golden apple snail on rice seedlings was 235.2 seconds, feeding length of that was 8.4cm. When golden apple snail fed rice seedlings, the stem remain only 2.5cm from areal part which could not recover. The more density of golden apple snail, the fester feeding rate. Density and number of eggs laid of golden apple snail after overwintering was higher on bank around of paddy field than on middle of that. The percent of shell size after over wintering was higher in small (below 25mm) than big (over 36mm). But in case of Boseong area, the slze was reverse because the soil has soft mud. The survival rate of overwintering golden apple snail was lower than that of breeding. They did not move when the temperature changed rapidly, but the temperature increased they start to move. They dead almost below $-3^{\circ}C$. The damage of rice by golden apple snail in the direct water seeding was 20%. In machine transplanting, rice damaged only fore-end of leaf and recovered subsequently. When golden apple snail release in direct water seeding field, the percent of damage was 5.6% in immediately release plot. The later release, the lower damage.

• Journal of the Korean Wood Science and Technology
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• v.45 no.5
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• pp.525-534
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• 2017

The study aims to observe the cellular anatomical properties of Dahurian larch and Japanese larch for the species identification. In addition, other factors - the ring width, tracheid length, density, and strength - were compared and analyzed to clarify their physical and mechanical properties. To the end, three Dahurian larch trees and three Japanese larch trees by each diameter class were collected as specimens from Jeongseon-eup, Jeongseon-gun, Gangwon-do, to conduct a stem analysis. It was found that the average stand age, average diameter at breast height, and average tree height of three Dahurian larch trees and Japanese larch trees were 74 years and 51 years, 442 mm and 352 mm, and 26.1 m and 20.8 m, respectively. The cellular anatomical difference between Dahurian larch and Japanese larch can be usually found by spiral thickening, considering that it doesn't occur in Dahurian larch at all, while rarely does in the ray tracheids of Japanese larch. However, in this study, spiral thickening was not observed in the radial section of Japanese larch. The average annual growth diameters measured at 1.2 m-height of Dahurian larch and Japanese larch were 5.167 mm and 5.954 mm, respectively. Meanwhile, arboreal growth of Japanese larch was observed to be higher than that of Dahurian larch. In the physical properties test, it was measured that the latewood proportion and oven-dry density of Dahurian larch with low annual diameter growth were higher than those of Japanese larch, while the mechanical properties of Dahurian larch wood were measured 2-7% higher than those of Japanese larch wood. The data obtained from this study are expected to be used as the basic reference for species identification between Dahurian larch and Japanese larch by DNA analysis.

• Korean Journal of Medicinal Crop Science
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• v.5 no.2
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• pp.95-101
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• 1997

This study was conducted to investigate how a labor saving for conventional hand sowing and respond to major agronomic traits under different planting density in 1993 and 1994. Each trial was a randomized block split plot with row spacing as the main plots and hill spacing as subplots. Sowing rate appeared lower in 20 or 25cm hill spacing using machine seeder than in hand sowing. Sowing time required reduced more 121 hours per hectare in 60cm row, 20cm hill spacings using machine seeder than in hand sowing. Increasing row and hill spacings reduced number of seedling stand per unit $area(m^2)$. Both number of pods per plant and grain weight per plant and showed highly increase in wide planting. Best yield obtained in 60cm row, 20cm hill spacing using machine seeder. Accumulative mean air temperature was positively correlated with stem height, number of pods per plant and yield, otherwise accumulative rainfall was negatively correlated with ones. A highly significant negative correlation was showed between number of seedling stand per unit $area(m^2)$ and number of branches, number of pods per plant, grain weight per plant and yield. Yield was positively correlated with number of branches, number of pods per plant and grain weight per plant.

• Journal of Korean Society of Forest Science
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• v.112 no.2
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• pp.127-135
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• 2023

This study aimed to develop biomass allometric equations and estimate carbon emission factors, such as the wood density, biomass-expansion factor, and root-to-shoot ratio, for Platanus occidentalis and Metasequoia glyptostroboides planted in urban areas. Twenty M. glyptostroboides and 25 P. occidentalis trees were harvested, and the dry weights and stem volumes of stems, branches, leaves, and roots (>5 mm) were measured. The wood densities of M. glyptostroboides and P. occidentalis were 0.293 ± 0.008 g cm^-3 and 0.509 ± 0.018 g cm^-3, and the biomass-expansion factors were 1.738 ± 0.031 and 1.561 ± 0.035. The root-to-shoot ratios were 0.446 ± 0.009 and 0.402 ± 0.012. The uncertainty tests (coefficient of variation, %) gave 2.8% and 3.5% values for wood density, 1.8% and 2.3% for biomass-expansion factor, and 2.1% and 2.9% for root-to-shoot ratio, respectively. Among the developed allometric equations, Model I using the diameter at breast height (DBH) was suitable. The allometric equations of M. glyptostroboides and P. occidentalis above ground were y = 1.679 (DBH)^1.315 and y = 0.505 (DBH)^1.896, and the allometric equations of the root and total were y = 0.746 (DBH)^1.315, y = 0.301 (DBH)^1.751, y = 2.422 (DBH)^1.316, and y = 0.787 (DBH)^1.858. If the carbon-emission factors of this study and biomass allometric equations of the three developed models are used to estimate the carbon storage and biomass of urban forests, errors caused by not considering the use of fixed factors and the environmental differences can be reduced.

• Korean Journal of Plant Resources
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• v.9 no.2
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• pp.151-155
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• 1996

For improvement germination rate of Cirsium setidens, wet seeds were storaged for 20 to 80 days at $4^{\circ}C$ and germinated at $18^{\circ}C$ in the dark or light condition. To assess the effects of shading and plant age on the growth, fresh yield and seed yield under rain shelter in highland at altitude of 800m above sea level, seeds were sown in an open field in late september,1990. In early May the next the seedlings were transplanted for vegetatable and seed production in the plastic houses for rain shelter. They were grown from 1991 to 1993 in the houses covered with the shading nets ranging from 0 to 70% for vegetable production and 0 to 50% for seed production. Germination rate inceased as the storage period lengthened, and the rate came to $60\sim80%$ at 60days after germination. It was more effective in lighting than in darkness. For vegetable production, the shading net treatments showed more values in almost the characters checked at the third year than the non-shading treatment. No. of stem, no, of leaves, stem length and degree of softening were no different between shading net treatments, but the other characters were significantly different. Ratio of survival, density of chlorophyll and ratio of dry matter of plants were highest in the plots of 50%, 70%, and 30% of shading, respectively. The highest fresh yield was recorded from 2-year plants, 70% of shading from them showed the greatest yield. The highest seed yields were produced 2-year-old plants of non-shading plot. For seed production, the non-shading treatment is best, and the highest seed yields were obtained in both the second and third years.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.32 no.4
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• pp.299-305
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• 2010

Purpose: Adipose tissue is located beneath the skin, around internal organs, and in the bone marrow in humans. Its main role is to store energy in the form of fat, although it also cushions and insulates the body. Adipose tissue also has the ability to dynamically expand and shrink throughout the life of an adult. Recently, it has been shown that adipose tissue contains a population of adult multipotent mesenchymal stem cells and endothelial progenitor cells that, in cell culture conditions, have extensive proliferative capacity and are able to differentiate into several lineages, including, osteogenic, chondrogenic, endothelial cells, and myogenic lineages. Materials and Methods: This study focused on endothelial cell culture from the adipose tissue. Adipose tissues were harvested from buccal fat pad during bilateral sagittal split ramus osteotomy for surgical correction of mandibular prognathism. The tissues were treated with 0.075% type I collagenase. The samples were neutralized with DMEM/and centrifuged for 10 min at 2,400 rpm. The pellet was treated with 3 volume of RBC lysis buffer and filtered through a 100 ${\mu}m$ nylon cell strainer. The filtered cells were centrifuged for 10 min at 2,400 rpm. The cells were further cultured in the endothelial cell culture medium (EGM-2, Cambrex, Walkersville, Md., USA) supplemented with 10% fetal bovine serum, human EGF, human VEGF, human insulin-like growth factor-1, human FGF-$\beta$, heparin, ascorbic acid and hydrocortisone at a density of $1{\times}10^5$ cells/well in a 24-well plate. Low positivity of endothelial cell markers, such as CD31 and CD146, was observed during early passage of cells. Results: Increase of CD146 positivity was observed in passage 5 to 7 adipose tissue-derived cells. However, CD44, representative mesenchymal stem cell marker, was also strongly expressed. CD146 sorted adipose tissue-derived cells was cultured using immuno-magnetic beads. Magnetic labeling with 100 ${\mu}l$ microbeads per 108 cells was performed for 30 minutes at $4^{\circ}C$ a using CD146 direct cell isolation kit. Magnetic separation was carried out and a separator under a biological hood. Aliquous of CD146+ sorted cells were evaluated for purity by flow cytometry. Sorted cells were 96.04% positivity for CD146. And then tube formation was examined. These CD146 sorted adipose tissue-derived cells formed tube-like structures on Matrigel. Conclusion: These results suggest that adipose tissue-derived cells are endothelial cells. With the fabrication of the vascularized scaffold construct, novel approaches could be developed to enhance the engineered scaffold by the addition of adipose tissue-derived endothelial cells and periosteal-derived osteoblastic cells to promote bone growth.

• Journal of fish pathology
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• v.2 no.2
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• pp.53-70
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• 1989

An ovarian parasite, Marteilioides chungmuensis of the Pacific oyster, Crassostrea gigas has been observed on several occasions in the Pacific sector of production of this oyster species(Matsuzato et al., 1977 ; Chun, 1979). This study was carried out on the specimens collected at Hwado, Och'$\check{o}$n, and Sinchang respectively located the southern, western, and eastern coasts of Korean Peninsula from 1986 through 1988 to investigate M. chungmuensis to the Pacific oyster. Uitrastructural studies were also carried out on infected oysters, to allow detailed examination of the structure and consepuently the systematic position of this parasite. Infection rates of M. chungmuensis at Hwado and Och'$\check{o}$n oyster farms were 5.3% and 4.2% each in 1986, 6.7% and 2.8% each in 1987, but they were not found at Sinchang oyster habitat. M,. chungmuensis-infected oysters were found from June to November at Hwado and from June to October at Och'$\check{o}$n. Twenty five of three hundred oysters transplanted from Sinchang to Hwado were found infected with M. chungmuensis. Some abnormal eggs infected with M. chungmuensis are liberated through the gill together with normal mature eggs on the spawning and the rest remain necrotized after spawning season. The earliest known stages consist of a stem cell or primary cell, including a secondary cell in which ovoid haplosporosomes are found. During sporulation, 2 or 3 secondary are produced by exogenous budding from the first secondary cell and, each secondary cell evolves into a sporont upon the tertiary cell differentiation (enodogenous budding) ; then, haplosporosomes are formed in the young sporont. Internal cleavages involve the differentiation of one tricellular spore per sporont. The outermost spore cell contains membrane-bounded osmiophilic bodies : the middle and the inner, most spore cells contain high density cytoplasmic ribosomes. The mechanism of spore formation from the stem cell of M. chungmuensis is the simplest of the class Paramyxea known up to now.

• Journal of Bio-Environment Control
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• v.14 no.2
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• pp.106-113
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• 2005

This study was carried out to develop a novel hydroponic medium far fruit vegetable crops by using waste synthetic fibers. In physical analysis of the synthetic fiber medium (SFM), the bulk density and percent solid phase were lower, while the porosity and water content were greater in comparison with the rockwool slab. The SFM had pH of 6.5 and EC of $0.03dS{\cdot}m^{-1}$ both of which are similar to those of the rockwool slab. The CEC of 0.39me/100mL of the SFM was lower than compared with 3.29me/100mL of the rockwool slab. However, concentrations K, Ca, Mg and Na were slightly higher in the SFM than those in the rockwool slab. The 'Momotaro' tomato crop in the SFM gave comparable plant height, stem diameter, days to first flowering, fruit weight and percent marketable yield as the rockwool slab. In the SFM and in the rockwool slab, mean fiuit weight were 182g and 181g, percent marketable yield were $93.8\%$ and $92.0\%$, respectively. The marketable yield per 10a in the SFM was 12,799 kg, which was $97\%$ of that in the rockwool slab. Growth parameters such as leaf length and width, leaf number, stem diameter and chlorophyll content of an exportable cucumber crop grown in the SFM and the rockwool slab were not different. Fruit weight was greater in the rockwool slab, while percent marketable yield was greater in the SFM. The marketable fruit yield per 10a of 5,062kg in the SFM was $2\%$ greater than that in the rockwool slab. $NO_3$ concentration in nutrient solution during the crop cultivation was higher in the SFM than in the rockwool slab, while concentrations $NH_4$, K, Ca, Mg and $SO_4$ were not different between the two media.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.2
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• pp.144-151
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• 2019

The establishment rate of foxtail millet seeds is greatly affected by soil environment conditions. To enhance germination viability and stable production of foxtail millet seeds, it is important to select seeds with a high density. Therefore, this study tested the selection of high-quality seeds using salt solution (specific gravity: 1.000, 1.005, 1.010, 1.015, 1.020, 1.025, 1.030, 1.035, and $1.040g\;L^{-1}$) and investigated their germination rates, percentages of emergence, and seeding quality. In this study, three varieties were tested: 'Hwanggeum', 'Samdachal', and 'Kyeongkwan1'. The thousand seed weight of all three varieties increased proportionally with specific gravity. The highest thousand seed weight was observed at the selection with $1.040g\;L^{-1}$ specific gravity, which also had the highest germination rate of 88.3%, 86.7%, and 90.6%, for 'Hwanggeum'; 'Samdachal'; and 'Kyeongkwan1', respectively. The results of seeding quality indicated that higher the specific gravity during selection, the higher were the values of plant growth (plant height, leaf length, stem diameter, root length, root weight, and stem weight). All the three varieties were found to be longer or heavier for seeds selected at the specific gravity of $1.040g\;L^{-1}$. The packing germination viability investigation found that higher the specific gravity for selection of seeds, the higher was the percentage of emergence (PE) and the emergence rate index (ERI). The PE was the highest for seeds selected at $1.040g\;L^{-1}$ specific gravity (85.3, 83.0, and 87.0%), and ERI was also as high as $2.82d^{-1}\;m^{-1}$ or more at $1.040g\;L^{-1}$. Selection of seeds with salt solution resulted in high germination viability of foxtail millet. Therefore, sowing seeds selected at $1.040g\;L^{-1}$ specific gravity is expected to help substantially in increased productivity.

• The Korean Journal of Zoology
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• v.14 no.2
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• pp.57-74
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• 1971

The present investigation has been done to observe the ecological habits of field mice to protect the rice from damages during the growing season in paddy-field and during the storge period. The results obtained are summarized as follows: 1. Of 155 mice captured in the period of April-November 1970, which belong to four genera (Apodemus, Cricetulus, Rattus, and Micromys), 148 mice(95%) were found as striped field mice (Apodemus agrarius coreae). The population density of striped field mouse was revealed by the present study as 55/ha, which is quite a low level compared with that in Japan of 900/ha. 2. The age distribution of the mice as judged by their body weight was found mainly composed of adult and the sex ratio was found to be 1.8 as determined with 147 individuals. The nest was found to be occupied by an adult and was composed of at least three openings and more than one food storage tunnels. The mice usually keep hulled rice rather than unhulled one in storage tunnel. The weight of food found in a nest was about 50 grams on an average. 3. The mice show a most active behaviour 1-2 hours after the sunset, around midnight, and an hour before the sunrise, but they are active even in daytime in order for searching for food and for breeding. 4. The ratio (%) of damage appeared in high stem of sweet corn in August was 30 ~ 40 percent, whereas that in low stem was 80 ~ 90 percent. The weight of spoiled grains in paddy-field was 11, 400gm/0.4ha and this gives an estimate of 349, 695 for whole country. 5. The female striped field mouse weighs average of about 30 grams and gives birth to average of 4.8 younglings which wean away from female mouse three weeks after delivery. 6. The natural enemies to the mice are found to be carnivores (weasel, cat, mountain cat, fox, raccoon, and otter), raptatores(eagle, owl, kete, buzzard), and snakes. Two kinds of field rats(Rattus norvegicus, Cricetulus tritor) are also the predator to the mice. 7. The feeding preference of striped field mice follows in decreasing order of sweet corn, soybean, sweet potatoes, chestnut, and wheat. The mice do not have a preference for barley, millet, rough millet, red bean, and green bean. 8. The starvation experiment, in which water alone was supplied, revealed that the mice in good physical and nutritional conditions survived for 71 ~ 79 hours, whereas those in worse conditions survived for only 32 ~ 39 hours.