• Title/Summary/Keyword: Stem density

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Short-term Effect of Thinning on Aboveground Carbon Storage in Korean Pine (Pinus koraiensis) Plantation (간벌이 잣나무 조림지 지상부 탄소저장량에 미치는 초기 영향)

  • Hwang, Jaehong;Bae, Sang-Won;Lee, Kyung Jae;Lee, Kwang-Soo;Kim, Hyun-Seop
    • Journal of Korean Society of Forest Science
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    • v.97 no.6
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    • pp.605-610
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    • 2008
  • This study was carried out to investigate the short-term (3 years) effect of thinning on aboveground carbon storage for 34-year-old (site 1) and 45-year-old (site 2) Korean pine (Pinus koraiensis Siebold et Zuccarini) plantations with different diameter class and site quality located in Gwangneung experimental forest. Thinning was manually carried out in consideration of basal area in 2004 (site 1 : 30% and 60% of basal area removed and site 2 : 60% of basal area removed). In 2004 and 2007, DBH and tree height were measured to analyze the changes in carbon storage after thinning. In the sites of 60% of basal area removed, although the mean DBH of site 1 was higher than that of site 2, mean annual carbon storage increment in site 2 ($6.5Mg\;C\;ha^{-1}yr^{-1}$) was about 3 times higher than that in site 1 ($2.3Mg\;C\;ha^{-1}yr^{-1}$). The reason for this result was probably due to higher stem density and site quality in site 2 compared to site 1. In site 2, mean annual carbon storage increment in thinned plot ($6.5Mg\;C\;ha^{-1}yr^{-1}$) was about 1.3 times higher than that in control ($5.2Mg\;C\;ha^{-1}yr^{-1}$). The results suggest that the stem density and site quality may be much more related to the aboveground carbon storage compared to diameter class. In addition, it is needed to consider these two factors for determining whether thinning is a feasible management alternative for the increase in aboveground carbon sequestration.

Evaluation of Early Productivity of High Density 'Fuji' Apple Orchards by Planting Well-feathered Trees/M.9 EMLA ('후지' 사과의 우량 측지묘/M.9 EMLA를 이용한 밀식재배원의 조기 생산성 평가)

  • Yang, Sang-Jin;Park, Moo-Yong;Song, Yang-Yik;SaGong, Dong-Hoon;Yoon, Tae-Myung
    • Horticultural Science & Technology
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    • v.28 no.3
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    • pp.374-380
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    • 2010
  • Well-feathered (5.2 feathers, stem diameter 13 mm) trees of 'Fuji' apple/ M.9 EMLA were planted at $4.0{\tiems}1.5$ m and whip trees (stem diameter 10 mm) of 'Fuji'/M.26 at $4.0{\times}2.0$ m were trained to the slender spindle. The productivity and yield efficiency of two orchard systems were compared for 6 years. The canopy volume of the tree/M.9 EMLA reached $2.07m^3$ in 2nd year and increased slowly to almost the targeted tree volume of $2.9m^3$ in 4th year. Trees/M.26 grew slowly at the begin but from 3rd year the tree volume expanded quickly to reach $5.6m^3$ in 5th year, covering over the allowed space. Yield of M.9 EMLA per 10a increased from 0.3 ton in 2nd year to 4.6 ton in 5th year, and 5.0 ton in 6th year, but yield of M.26 per 10a increased from 0.5 ton in 3rd to 2.9 ton in 6th year. Cumulative yield per 10a up to 6th year was 13.9 ton for M.9 EMLA but only 9.8 ton for M.26. Fruit weight for M.9 EMLA was heavier than that for M.26. In conclusion, the high density planting system with well-feathered trees/M.9 EMLA was better than the conventional wide planting system with whip trees/M.26.

EFFECT OF ENAMEL MATRIX DERIVATIVE (EMD, $EMDOGAIN^{(R)}$) ON THE DIFFERENTIATION OF CULTURED HUMAN PERIODONTAL LIGAMENT CELLS AND MESENCHYMAL STEM CELLS (배양된 사람 치주인대세포와 골수유래간엽줄기세포의 분화에 미치는 법랑기질유도체 (Enamel Matrix Derivative, EMD)의 영향)

  • Park, Sang-Gyu;Jue, Seong-Suk;Kwon, Yong-Dae;Choi, Byung-Joon;Kim, Young-Ran;Lee, Baek-Soo
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.31 no.4
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    • pp.281-286
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    • 2009
  • Introduction: Enamel matrix derivative (EMD) is a protein which is secreted by Hertwig root sheath and plays a major role in the formation of cementum and attachment of peridontium. Several studies have shown that EMD promoted the proliferation and differentiation of preosteoblasts, osteoblasts and periodontal ligament cells in vitro: however, reports showing the inhibition of osteogenic differentiation by EMD also existed. This study was designed to simultaneously evaluate the effect of EMD on the two cell lines (human mesenchymal stem cells: hMSC, human periodontal ligament derived fibroblasts: hPDLCs) by means of quantitative analysis of some bone related matrices (Alkaline phosphatase : ALP, osteopontin ; OPN, osteocalcin ; OC). Materials and Methods: hMSCs and hPDLCs were expanded and cells in the 4${\sim}$6 passages were adopted to use. hMSc and hPDLCs were cultured during 1,2,7, and 14 days with 0, 50 and 100 ${\mu}g/ml$ of EMD, respectively. ALP activity was assessed by SensoLyte ALP kit and expressed as values of the relative optical density. Among the matrix proteins of the bony tissue, OC and OPN were assessed and quantification of these proteins was evaluated by means of human OC immunoassay kit and human OPN assay kit, respectively. Results: ALP activity maintained without EMD at $1,2^{nd}$ day. The activity increased at $7^{th}$ day but decreased at $14^{th}$ day. EMD increased the activity at $14^{th}$ day in the hPDLCs culture. In the hMSCs, rapid decrease was noted in $7^{th}$ and $14^{th}$ days without regard to EMD concentrations. Regarding the OPN synthesis in hPDLCs, marked decrease of OPN was noted after EMD application. Gradual decrease tendency of OPN was shown over time. In hMSCs, marked decrease of OPN was also noted after EMD application. Overall concentration of OPN was relatively consistent over time than that in hPDLCs. Regarding the OC synthesis, in both of hPDLCs and hMSCs, inhibition of OC formation was noted after EMD application in the early stages but EMD exerted minimal effect at the later stages. Conclusion: In this experimental condition, EMD seemed to play an inhibitory role during the differentiation of hMSCs and hPDLCs in the context of OC and OPN formation. In the periodontium, there are many kinds of cells contributing to the regeneration of oral tissue. EMD enhanced ALP activity in hPDLCs rather than in hMSCs and this may imply that EMD has a positive effect on the differentiation of cementoblasts compared with the effect on hMSCs. The result of our research was consistent with recent studies in which the authors showed the inhibitory effect of EMD in terms of the differentiation of mineral colony forming cells in vitro. This in vitro study may not stand for all the charateristics of EMD; thus, further studies involving many other bone matrices and cellular attachment will be necessary.

Classification of Quercus mongolica Stand Types at Mt. Joongwang, Kangwon-Do and Determination of Proper Future Tree Density for Forest Tending Work (중왕산(中旺山) 지역(地域) 신갈나무림(林)의 임분형(林分形) 구분(區分) 및 육림작업(育林作業)에 적절(適切)한 미래목(未來木) 본수(本數)의 결정(決定))

  • Choi, Seon Deok;Lee, Don Koo;Ma, Sang Kyu
    • Journal of Korean Society of Forest Science
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    • v.87 no.4
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    • pp.631-641
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    • 1998
  • The objectives of this study were 1) to classify the types of Quercus mongolica stands at Mt. Joongwang and compare their quality, and 2) to determine the proper future tree number of Q. mongolica per ha and the appropriate distance between the future trees. The results from this study were as follows : Q. mongolica stands at Mt. Joongwang was classified into four types, pure Q. mongolica stand as stand type I, Q. mongolica - hardwood stand as stand type II, Q. mongolica - Pines densiflora stand as stand type III, Hardwood - Q. mongolica stand as stand type IV, according to mixture rate in stand volume. Stand type IV showed the best quality stem of Q. mongolica among the stand types, and the stem quality of Q. mongolica in Q. mongolica stand mixed with hardwood as stand types II and IV was better than those in pure Q. mongolica stand as stand type I and in Q. mongolica - P. densiflora stand as stand type III. If the management goal for Q. mongolica stand is to produce its high quality-timber, it is desirable to sustain proper mixture rate of Q. mongolica with another hardwoods. The proper number of future trees in pure Q. mongolica stand as stand type I was 122trees/ha and reasonable distance between the future trees was 9.15m. The distance between future trees in other stand types was 7.2m to 9.3m for stand types II and IV, while 8.0m for stand type III. Thus, the classification of Q. mongolica stand type based on stand character and maturity, and proper stem number of future tree and optimum distance between future trees would be a useful forest tending work.

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The Growth Performances and Soil Properties of Planted Zelkova serrata Trees according to Fertilization in Harvested Pinus rigida Plantation over 6 Years after Planting (조림지 시비 처리에 따른 리기다소나무 벌채지 내 식재 6년 후 느티나무 조림지 토양 및 조림목 생장 특성)

  • Yang, A-Ram;Cho, Min Seok
    • Journal of Korean Society of Forest Science
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    • v.108 no.1
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    • pp.29-39
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    • 2019
  • The objective of this study was to suggest a suitable amount of fertilizer using the changes in growth performances and soil properties for improving survival and quality of Zelkova serrata trees in a harvested Pinus rigida plantation. One-year-old containerized seedlings of Z. serrata were planted with the density of 3000 seedlings $ha^{-1}$ in end of March 2011 at Gwangneung experimental forest, Pocheon. Solid compound fertilizer (N:P:K=3:4:1) were applied yearly in three amounts (control: no fertilization, F1: $180kg\;ha^{-1}$, and F2: $360kg\;ha^{-1}$) every May from 2011 to 2013. We analyzed soil properties before (2011) and after (2012 and 2017) fertilization. And we measured the root collar diameter and height of Z. serrata trees from 2011 to 2016, and then calculated H/D ratio and stem volume. Soil properties at Z. serrata plantation did not show difference according to fertilization level in every investigation year. As time passed after planting, however, concentrations of total nitrogen and available phosphorus were increased from decreased. The growth of root collar diameter, height and stem volume of Z. serrata trees at F2 plot were significantly higher those at the other plots after only 2 years of fertilization. Because Z. serrata tree demand to more nutrient during the early growing period. The survival rate of Z. serrata trees at control plot was significantly lower than that at the other plots. This might be due to Z. serrata trees at control plot had not the upper hand from competition with vegetation at the early in planting. However, the growth of height and stem volume of Z. serrata trees between F1 and F2 plots did not show difference over 6 years after planting. Consequently, we could suggest that Z. serrata trees need to F1 fertilization level for considering improving survival and quality of Z. serrata trees and economical efficiency of plantation managements after harvesting P. rigida plantation.

Dual Plate Fixation for Periprosthetic Femur Fracture after Total Knee Arthroplasty (슬관절 전치환술 후 발생한 대퇴골 삽입물 주위 골절의 이중 금속판 고정술)

  • Kim, Dong Hwi;Cha, Dong Hyuk;Ko, Kang Yeol
    • Journal of the Korean Orthopaedic Association
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    • v.56 no.1
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    • pp.26-33
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    • 2021
  • Purpose: This study evaluated the results of dual plate fixation for periprosthetic femur fracture after total knee arthroplasty (TKA). Materials and Methods: From October 2007 to February 2013, 23 cases of periprosthetic femur fracture after TKA were treated at the author's hospital. There were 13 cases of fixation using a medial and lateral dual plate when the stability of the fracture site could not be achieved by one side fixation with a follow-up of more than one year. The cases included no loosening of the femoral component in fractures that were categorized as Lewis-Rorabeck classification II and supracondylar comminuted fractures and elongation of the fracture line to the lateral epicondyle of the femur or stem in the medullary canal. The mean age was 72 years (65-82 years), and 11 cases were female. Three cases had a stem due to revision. The mean bone marrow density was -3.2 (-1.7 to -4.4), and the mean period from primary TKA to periprosthetic fractures was 28 months (1-108 months). The mean follow-up period was 23 months (12-65 months). The medial fracture site was first exposed via the subvastus approach. Second, the supplementary plate was fixed on the lateral side of the fracture using a minimally invasive plate osteosynthesis technique. The average union time, complications, and Hospital for Special Surgery Knee Score (HSS) at the last follow-up were evaluated. Results: The mean union time was 17.4 weeks (7-40 weeks). Two cases showed delayed bone union and nonunion occurred in one case, in whom bone union was achieved three months later after re-fixation using a dual plate with an autogenous bone graft. The mean varusvalgus angulation was 1.67 degrees (-1.2-4.9 degrees), and the mean anterior-posterior angulation was 2.86 degrees (0-4.9 degrees) at the last follow-up. The mean knee range of motion was 90 degrees, and the HSS score was 85 points (70-95 points) at the last follow-up. Conclusion: Dual plate fixation for periprosthetic femur fractures that had not achieved stability by one side plate fixation after TKA showed a good clinical result that allowed early rehabilitation.

The Variation of Natural Population of Pinus densiflora S. et Z. in Korea (V) -Characteristics of Needle and Wood of Injye, Jeongsun, Samchuk Populations- (소나무 천연집단(天然集團)의 변이(變異)에 관(關)한 연구(硏究)(V) -인제(麟蹄), 정선(旌善), 삼척집단(三陟集團)의 침엽(針葉) 및 재질형질(材質形質)-)

  • Yim, Kyong Bin;Kwon, Ki Won;Lee, Kyong Jae
    • Journal of Korean Society of Forest Science
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    • v.36 no.1
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    • pp.9-25
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    • 1977
  • As a successive work of the variation studies of natural Pinus densiflora stands, some characteristics of individual trees of the three natural populations selected from the Kwang-won Province, the middle-east part of Korean peninsula, as shown in the location map, were investigated. And the statiscal differences between individuals within population, and between populations were analysed. Twenty trees from each population were selected for this study purpose. Doing this, those trees lagged in growth, usually showing poorer form, were eliminated. The results obtained are summarized as follows: 1. Though the average population ages had the ranage between 50 and 63, the growth of height or diameter was similar. Population No.9 is, however, considered to have better tree forms at glance. Population No.8 showed the heighest value not only in the clear-stem-length ratio. 0.53 but also in the crown-index 0.91. The higher value can be result from those trees having long lateral branches and relatively short crown height, meaning undesirable crown shape. In regard to the fine branchedness and the acuteness of branching angle, the population No.9. is considered to be a better one, whereas there was almost no difference in crown height among populations. 2. Checking the frequency distributions of the ratio of the clear-stem-height to the total height and the crown-indices, some difference between populations are considered. These might be attributed to the previous way of stand mangement which alters the density. 3. In the serration density, the average number of 54 per 1cm needle length, the significant differences exist between individual trees within population but not between populations. A few trees which extremly high serration density were observed. As in serration, so tendencies were in the number of stomata row and resin duct. 4. The population 8 had the resin duct index value of 0.074 as the highest which was twice or triple of the other ones. 5. The patterns of increasing process of the average 10-year-ring-segment were not similar till the 30 years of age, but beyond this, the tendency lines were aggregated. 6. Regading the average summer wood ratio, no diffrence between populations, but in the ranges, i.e. 23 to 30 in population No.8. and 16 to 36 in population No.9., with regad to the specific gravity of wood, there were hardly observed any difference between populations even in the ranges values. As the increase of tree ages, the increase of specific gravity was followed but the increasing patterns were not similar between populations. 7. No significant differences between populations in the average tracheid length and the range were detected. However, the length was increased according to the age increase. The increasing pattern was same between populations.

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Studies on the growth of Korea Lawn Grass (Zoysia japonica Steud.)in Reponse to Nitrogen Application, Clipping Treatment and Plant Density (질소시용, 예초 및 재식밀도가 한국잔디(Zoysia Japonica Steud)의 생육에 미치는 영향)

  • Sim, Jae-Seong
    • The Journal of Natural Sciences
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    • v.1
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    • pp.61-113
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    • 1987
  • The increasing emphasis placed on the production of fine turf for lawns, golf courses, parks, and other recreational sites has led to many unsolved problems as to how such turf could be best established and mainteined. For this purpose, a series of experiments were conducted under con ditions of pot and field. The results obtained were as follows EXPERIMENT I. The effect of nitrogen fertilizer and clipping interval on Zoysia japonica. 1. Increasing the rate of nitrogen and frequent clipping increased tiller number of Zoysis japonica and the maximum number of tillers were obtained from 700 kg N application and freqnent clippings (10 days interval ) in October. Treatment of 350kg N with 10 days clipping interval increased tillers much more than those of 700 kgN with 20 and 30 days clipping intervals. 2. The average number of green leaves occurred during the growth period maximized by applying 700 kg N and clipping 10 days interval. 3. Increasing tiller numbers significantly decreased tops DM weight per tiller by clipping plants at interval of 10 and 20 days, irrespective of nitrogen applied, and with nil N, at the interval of 30 days. By applying 700 kg N, however, top DM weight per tiller increased as the number of tillers increased consistently. 4. The highest top DM weight was achieved from late August to early September by applying 350 and 700kgN. 5. During the growth period, differences in unders ( stolon + root ) DM weight occurred bynitrogen application were found between nil N and two applied nitrogen levels, whereas, at the same level of nitrogen applied, the increase in stolon DM weight enhanced by lengthening the clipping interval to 30 days. 6. Nitrogen efficiency to green leaves, stolon nodes and DM weight of root with high nitrogen was achieved as clipping interval was shortened. 7. By increasing fertilizer nitrogen rate applied, N content n the leaves and stems of Zoysiajaponica was increased. On the other hand, N content in root and stolon had little effect onfertilizer nitrogen, resulting in the lowest content among plant fractions. The largest content of N was recorded in leaves. Lengthening the clipping interval from 10 or 20 to 30 days tends to decrease the N content in the leaves and stems, whereas this trend did not appeared in stolon androot. 8. A positive correlations between N and K contents in tops and stolon were established andthus K content increased as N content in tops and stolon increased. Meanwhile, P content was not affected by N and clipping treatments. 9. Total soluble carbohydrate content in Zoysia japonica was largest in stolon and stem, and was reduced by increasing fertilizer nitrogen rate. Reduction in total soluble carbohydrate due to increased nitrogen rate was severer in the stolons and stems than in the leaves. 10. Increasing the rate of nitrogen applied increased the number of small and large vascular bundles in leaf blade, but shortened distance among the large vascular bundles. Shortening the clipping interval resulted in increase of the number of large vascular bundles but decrease ofdistance between large vascular bundles.EXPERIMENT II. Growth response of Zoysia japonica imposed by different plant densities. 1. Tiller numbers per unit area increased as plant density heightened. Differences in num ber between densities at higher densities than 120 D were of no significance. 2. Tiller numbers per clone attained by 110 days after transplanting were 126 at 40D,77 at 80D, 67 at 120D, 54 at 160D, and 41 at 200D. A decreasing trend of tiller numbers per clone with increasing density was noticable from 100 days after transplanting onwards. 3. During the growth period, the greatest number of green leaves per unit area were attainedin 90days after transplanting at 160D and 200D, and 100 days after transplanting at 40D, 80Dand 120D. Thus the period to reach the maximum green leaf number with the high plantdensity was likely to be earlier that with the low plant density. 4. Stolon growth up to 80 days after transplaning was relatively slow, but from 80 daysonwards, the growth quickened to range from 1.9 m/clone at 40D to 0.6m/clone at 200Din 200 days after transplanting, these followed by the stolon node produced. 5. Plant density did not affect stolon weight/clone and root weight/clone until 80 daysafter transplanting. 6. DM weight of root was heavier in the early period of growth than that of stolon, butthis trend was reversed in the late period of growth : DM weight of stolon was much higherthan that of root.EXPERIMENT Ill. Vegetative growth of Zoysia japonica and Zoysia matrella as affected by nitrogen and clipping height. 1. When no nitrogen was applied to Zoysia japonica, leaf blade which appeared during theAugust-early September period remained green for a perid of about 10 weeks and even leavesemerged in rate September lived for 42 days. However, leaf longevity did not exceed 8 weeks asnitrogen was applied. In contrast the leaf longevity of Zoysia matrella which emerged during the mid August-earlySeptember period was 11 weeks and, under the nitrogen applied, 9 weeks, indicating that thelife-spen of individual leaf of Zoysia matrella may be longer than that of Zoysia japorica. Clipping height had no effect on the leaf longevity in both grasses. 2. During the July-August period, tiller number, green leaf number and DM weightof Zoysia japonica were increased significantly with fertilizer nitrogen, but were not with twolevel of clipping height. This trend was reversed after late September ; no effect of nitrogen wasappeared. Instead, lax clipping increased tiller number, green leaf number and DM weight. Greenleaves stimulated by lax clipping resulted in the occurrance of more dead leaves in late October. 3. Among the stolons outgrown until early September, the primary stolon was not influencedby nitrogen and clipping treatments to produce only 2-3 stolons. However, 1st branch stoIon asaffected by nitrogen increased significantly, so most of stolons which occurred consisted of 1st branch stolons. 4. Until early September, stolon length obtained at nil nitrogen level was chiefly caused bythe primary stolons. By applying nitrogen, the primary stolons of Zoysia japonica waslonger than 1st branch stolons when severe clipping was involved and in turn, shorter than 1stbranch stolons when lax clipping was concerned. In Zoysia matrella, 1st branch stolons were muchlonger than the primary stolon when turf was clipped severely but in conditions of lax clippingthere was little difference in length between primary and 1st branch stolons. 5. Stolon nodes of both Zoysia japonica and Z. matrella were positively influenced by nit rogen, but no particular increase by imposing clipping height treatment was marked in Zoysiamatrella. Although the stolon of Zoysia japonica grew until late October, the growthstimulated by nitrogen was not so remarkable as to exceed that by nil N.

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Studies on Planting Density and Labor - Saving in Machine Sowing for Astragalus membranaceus Bunge (황기 기계파종시(機械播種時)의 적정(適正) 재식밀도(栽植密度)와 성력효과(省力效果))

  • Kim, Young-Guk;Chang, Young-Hee;Lee, Seung-Tack;Yu, Hong-Seob
    • Korean Journal of Medicinal Crop Science
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    • v.4 no.2
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    • pp.157-162
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    • 1996
  • Experiments were conducted from 1994 to 1995 to understand the effects of the labor-saving seeding and planting density on growth and root yield of Astragalus membranaceus. The drilling seeder reduced seeding time than the hand seeding; It takes 3. 5hrs/l0a to seed by drilling seeder while 33. 0hrs/l0a by hand seeding, which labor reducing rate was 89. 4 %. Emergence rate in the drilling seeder was increased 17% than in the hand seeding, so the root yield were increased 23% to 136. lkg/l0a in using drilling seeder compared to 110. 3kg in hand seeding. On the effect of planting density on the growth characteristics plant height was long in dense planting and stem diameter was thick in spacious planting. Root diameter and dry root weight root per plant were decreased in dense planting and root yield was highest in optimum planting densities $(6\;row\;(15cm)\;{\times}\;10cm)$ in the harvest of 1 year old plants in Astragalus membranaceus. The gross profit were increased 23% to 1,933 thousand won per l0a in the drilling seeder compared to 1,566 thousand won in the hand seeding, also the managing costs were reduced 18% to 406 thousand won per l0a in the drilling seeder than 494 thousand won per l0a in the hand seeding.

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Occurrence and Damage of Golden Apple Snail (Pomacea canaliculata : Ampullariidae) in Jeonnam Province of South Korea (전남지역 왕우렁이(Pomacea canaliculata: Ampullariidae)의 발생생태 및 피해)

  • Kim, Do-Ik;Kim, Seon-Gon;Choi, Kyeong-Ju;Kang, Beom-Ryong;Park, Jong-Dae;Kim, Jeong-Jun;Choi, Dong-Ro;Park, Hyeong-Man
    • Korean journal of applied entomology
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    • v.46 no.1 s.145
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    • pp.109-115
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    • 2007
  • This study was carried out to determine occurrence and damage of golden apple snail, Pomacea canaliculata on water seeding area in Jeonnam province. The density of golden apple snail maintained hlgh until October 2003, but quickly decreased from November. They overwintered only in a portion of the water canals but could not find in dried paddy field. They reproduced from May and June in the canal water and paddy field, respectively. Feeding time of golden apple snail on rice seedlings was 235.2 seconds, feeding length of that was 8.4cm. When golden apple snail fed rice seedlings, the stem remain only 2.5cm from areal part which could not recover. The more density of golden apple snail, the fester feeding rate. Density and number of eggs laid of golden apple snail after overwintering was higher on bank around of paddy field than on middle of that. The percent of shell size after over wintering was higher in small (below 25mm) than big (over 36mm). But in case of Boseong area, the slze was reverse because the soil has soft mud. The survival rate of overwintering golden apple snail was lower than that of breeding. They did not move when the temperature changed rapidly, but the temperature increased they start to move. They dead almost below $-3^{\circ}C$. The damage of rice by golden apple snail in the direct water seeding was 20%. In machine transplanting, rice damaged only fore-end of leaf and recovered subsequently. When golden apple snail release in direct water seeding field, the percent of damage was 5.6% in immediately release plot. The later release, the lower damage.