• The Plant Pathology Journal
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• 제30권2호
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• pp.215-219
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• 2014

The GacS/GacA two component system regulates various traits related to the biocontrol potential of plant-associated pseudomonads. The role of the sensor kinase, GacS, differs between strains in regulation of motility. In this study, we determined how a gacS mutation changed cell morphology and motility in Pseudomonas chlororaphis O6. The gacS mutant cells were elongated in stationary-phase compared to the wild type and the complemented gacS mutant, but cells did not differ in length in logarithmic phase. The gacS mutant had a two-fold increase in the number of flagella compared with the wild type strain; flagella number was restored to that of the wild type in the complemented gacS mutant. The more highly flagellated gacS mutant cells had greater swimming motilities than that of the wild type strain. Enhanced flagella formation in the gacS mutant correlated with increased expression of three genes, fleQ, fliQ and flhF, involved in flagellar formation. Expression of these genes in the complemented gacS mutant was similar to that of the wild type. These findings show that this root-colonizing pseudomonad adjusts flagella formation and cell morphology in stationary-phase using GacS as a major regulator.

• Bulletin of the Korean Chemical Society
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• 제27권11호
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• pp.1775-1779
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• 2006

A liquid chromatographic chiral stationary phase (CSP) based on (+)-(18-crown-6)-2,3,11,12-tetracarboxilic acid without extra free aminopropyl groups on silica surface has been demonstrated to be quite effective for the resolution of various $\beta$-amino acids. The retention factors ($k_1$) for the resolution of $\beta$-amino acids on the CSP were quite large and the large retention factors might be quite attractive along with the reasonable separation factors ($\alpha$) for preparative scale enantioselective chromatography. The large retention factors on the CSP were found to be reduced effectively by adding ammonium ion to mobile phase without sacrificing the chiral recognition efficiency of the CSP. Consequently, the CSP is also quite applicable for use in analytical enantioselective chromatography.

• Bulletin of the Korean Chemical Society
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• 제27권8호
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• pp.1222-1224
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• 2006

• Bulletin of the Korean Chemical Society
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• 제29권11호
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• pp.2135-2139
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• 2008

• Bulletin of the Korean Chemical Society
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• 제25권3호
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• pp.400-402
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• 2004

• Bulletin of the Korean Chemical Society
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• 제29권9호
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• pp.1850-1852
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• 2008

• 대한조선학회지
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• 제27권3호
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• pp.11-18
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• 1990

Kelvin의 principle of stationary phase는 cusp point에서는 사용불능이며 이 cusp point근처에서 nonuniform expanision을 준다. 이 nonuniform expanision을 이용해서 cusp point 근처에서 유용한 uniform expanision을 도출하는데 본 논문의 목적이 있다.

• Bulletin of the Korean Chemical Society
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• 제31권10호
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• pp.2943-2948
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• 2010

Silica monolith powders were prepared by a new procedure where ground powders of proper size distribution were obtained without sieving. An initiator was attached to this ground monolith and polystyrene was bound by reversible addition-fragmentation chain transfer polymerization to give a new stationary phase. The separation efficiency of this phase was found better than that of the polystyrene bound phase based on conventional silica particles and that of the C18 bound silica monolith powders.

• Bulletin of the Korean Chemical Society
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• 제7권1호
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• pp.45-50
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• 1986

Separation of optical isomers of some derivatives of amino acids by reversed-phase HPLC has been accomplished by adding a chelate of an optically active amino acid to copper(Ⅱ) to the mobile phase. Cu(Ⅱ) complexes of L-proline and L-hydroxyproline in the mobile phase showed different degrees of separation. Optical isomers of DNS derivatives of amino acids are selectively separated, but those of several other derivatives are not at all. The kinds of buffer agents, the pH, and the concentrations of acetonitrile and the Cu(Ⅱ) ligand all affect the separations. The elution behavior between D and L DNS-amino acids appears to depend on the alkyl side chain of the amino acids. A chromatographic mechanism is proposed that is based on a stereospecificity of the formation of ternary complexes by the D, L-DNS-amino acids and the chiral additive associated with the stationary phase. The steric effects of the ligand exchange reactions are related with the feasibility of cis and/or trans attack of the amino acids to the binary chiral chelate retained on the stationary phase.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 1996년도 춘계학술대회 논문집
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• pp.403-413
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• 1996

Two-phase self-organizing neuro-modeling (SONM). the global SONM and local SONM, is designed for tracking non-stationary manufacturing processes. Radial basis function (RBF) neural network is employed, and self-tuning estimator is also developed for the determination of RBF network parameters on-line. A pattern recognition approach is presented for identifying a correct RBF neural network, which is used for identifying current manufacturing processes. Experimental results showed that the proposed approach is suitable for tracking non-stationary processes.