• Title/Summary/Keyword: Stationary phase

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Layout Principles of Renaissance Classicism Architectural Style and Its Application on Modern Fashion Design - Focused on Classic Style Fashion after the Year 1999 - (르네상스 고전주의 건축양식의 조형원리와 현대패션디자인에의 적용 - 1999년 이후 클래식 스타일 패션을 중심으로 -)

  • Lee, Shin-Young
    • The Research Journal of the Costume Culture
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    • v.18 no.2
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    • pp.261-276
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    • 2010
  • The analysis of an art trend in the principle dimension starts by observing the object of work in the perspective of formative composition and recognizing it as a universal system. It can be said that it is consistent with an interpretation method for a form theory of formal history by Heinrich W$\ddot{o}$lfflin, a leading form critic in art criticism. Hence, the purpose of this study was to find out what are the formative principles in Renaissance Classicism as a design principle to be applicable to modern fashion by reviewing the formative characteristics of Renaissance Classicism Architecture with which W$\ddot{o}$lfflin directly dealt. As for the theoretical literature review, I used W$\ddot{o}$lfflin's theoretical framework and looked at the Renaissance Classicism Architecture that he studied and examined the possibility of utilizing his theory as a layout principle and the characteristics. As for analysis of design cases, I applied the aforementioned architecture layout principle to modern fashion and conducted case study analysis to delve into distinctive layout principles found in fashion. The study showed that the Renaissance Classicism Architectural Style is marked by linearity, planarity, closing and multiple unity: linearity was expressed in the observation form in fixed frontal view and an emphasis on a tangible silhouette homeogenous and definite line structures; planarity was achieved in the form of paralleled layers of frontal view element, planarity style, and identical and proportional repetition of various sizes.; closing signified the pursuit of complete and clear regularity, and architecture developed in a constructive phase through organizational inevitability and absolute invariability.; multiple unity was expressed in self-completedness and independent parallel of discrete forms and harmony of emphasized individual elements in a totality. Applying these layout characteristics of the Renaissance Classicism Architectural style and to see their individual expressive features, I found out that in adopting layout principles of the Renaissance Classicism Architecture to modern fashion, it turned out to be an emphasis of individual silhouettes, a flattened space, completed objects, organic harmony among independent parts: the emphasis of individual silhouettes was expressed in individual definitiveness of formative lines of clothes in accordance with body joints and an emphasis on formative lines of clothes; the flattened space was marked by single layer structure, planarity of elements of clothes, and listing arrangement by appropriate proportion.; the completedness of the objects was expressed by the stationary state where overall image is fixed, the construction of homogeneous and complete space, and absolute inevitability of internal layout in proportion; lastly, organic harmony of independent parts was stressed in independent completedness of each detail, and organic harmony of the whole. The expressive features would lead to a unique expression style of linear emphasis, proportion, constructive forms, and two-dimensional arrangement. The meaning of this study is follows: The characteristics of art school of thought are given shape by appling & analysing the architectural layout principles of historical art school of thought to modern fashion in the view point of formal construction dimension. The applied possibility of historical art school of thought as the source of inspiration about the fashion design is extended.

Elimination of Escherichia coli O157:H7 Contaminated in Frozen Beef by Electron Beam Irradiation (전자선 조사에 의한 동결육에 오염된 Escherichia coli O157:H7 의 제거)

  • Kwon, Oh-Jin;Yang, Jae-Seung;Lim, Seong-Il;Byun, Myung-Woo
    • Korean Journal of Food Science and Technology
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    • v.29 no.4
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    • pp.771-775
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    • 1997
  • Treatment with electron beam irradiation was investigated for the elimination of Escherichia coli O157:H7 which has been linked to outbreaks of foodborne illness on undercooked and raw meat. Before treatment, the maximum populations were observed at 16 hr when E. coli O157:H7 was incubated in TSB at $37^{\circ}C$. Incubation at $4^{\circ}C$ did not influence survival and growth of the strain. The numbers of E. coli O157:H7 were present about $10^{7}\;CFU/mL$ in the log $(6\;hr\;at\;37^{\circ}C)$ and stationary phase $(16\;hr\;at\;37^{\circ}C)$ of cells, respectively. Freezing $(24\;hr\;at\;-18^{\circ})$ had a more marked lethal effect. The $D_{10}$ value at $-18^{\circ}C$ of E. coli O157:H7 contaminated in frozen beef was 0.45 kGy, and inactivation factor were $6.67{\sim}11.11$ at the radiation doses of $3{\sim}5\;kGy$. Therefore, electron beam irradiation was an effective method to eleminate of E. coli O157:H7.

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Production of Biosurfactant by Pseudomonas sp. SW1 for Microbial Remediation of Oil Pollution (유류오염방제를 위한 Pseudomonas sp. SW1로부터 생물계면활성제의 생산)

  • Son, Hong-Joo;Suk, Wan-Su;Lee, Geon;Lee, Sang-Joon
    • Korean Journal of Microbiology
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    • v.33 no.3
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    • pp.193-198
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    • 1997
  • Microorganisms capable of producing biosurfactant were isolated from oil-contaminated soils and seawater. Among them, the selected strain SW1 was identified as Pseudomonas sp. by taxonomical characteristic tests, and so tentatively named Pseudomonas sp. SWI. The optimal temperature and initial pH for biosurfactant production were TEX>30^{\circ}C.$ and 7.0, respectively. The optimal medium composilion for the production of biosurfactant by Pseudomonas sp. SW1 were hexadecane of 2.0%, yeast extract of 0.04%, $K_{2}HPO_4$ of 0.02%, $KH_2PO_4$ of 0.03% and $MgSO_4$ center dot $7H_2O$ of 0.04%, respectively. Under the above conditions, minimum wrface tension was 32 mN/m after incubation of 2 days. The biosurfactant was produced during initial stationary phase in the optimal medium. Pseudotnonas sp. SWl utilized various hydrocarbons such as Bunker oils, n-alkanes and branched alkanes as a sole carbon source.

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Extracellular Polysaccharide Produced by a New Methylotrophic Isolate (새로운 메탄올 자화세균이 생산하는 세포외 다당류)

  • Lee, Ho J.;Kim, Si W.;Kim, Young M.
    • Korean Journal of Microbiology
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    • v.34 no.4
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    • pp.212-218
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    • 1998
  • An obligately methylotrophic bacterium which produces extracellular polysaccharide (EPS) was isolated through methanol-enrichment culture technique. The isolate was aerobic, nonmotile, and gram negative rod and exibited catalase, but no oxidase, activity. Plasmid, carotenoid, and poly-${\beta}$-hydroxybutyric acid were not found. The guanine plus cytosine content of DNA was 52-56%. The isolate was found to grow only on methanol and monomethylamine. Growth was optimal ($t_d=2.4h$) at $35^{\circ}C$ and pH 6.5 in a mineral medium containing 0.5% (v/v) methanol, 25 mM phosphate, and 0.212% ammonium sulfate. Methanol was assimilated through the ribulose monophosphate pathway. Maximun amount of EPS was produced in cells growing at the mid-stationary growth phase at $30^{\circ}C$ in a mineral medium (PH 6.5) containing 1.0% (v/v) methanol in the CIN ratio of 54.7. Thin-layer chromatographic and high performance liquid chromatographic analysis revealed that the EPS was composed of glucose and galactose. EPS which was not treated with ethanol (Pbe) exhibited stable viscosity under various concentrations of salts and temperatures hut showed high viscosity at low pH. EPS precipitated with ethanol (Pae) was found to be more stable in viscosity than the Pbe at various salt concentrations, temperatures, and pH. The Pae also exhibited higher viscosity than the Pbe and xanthan gum. Scanning electron microscopy revealed that the lyophilized Pbe and Pae have a multi-layered structure and a structure of thick fibers, respectively.

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Production of fermented apple juice using Lactobacillus plantarum JBE245 isolated from Korean traditional Meju (메주에서 분리한 Lactobacillus plantarum JBE245를 이용한 사과 발효 음료 제조)

  • Heo, Jun;Park, Hae-Suk;Uhm, Tai-Boong
    • Korean Journal of Food Science and Technology
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    • v.48 no.5
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    • pp.445-453
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    • 2016
  • Eighty-four strains of lactic acid bacteria were isolated from Korean fermented foods for the production of fermented apple juice. Among these strains, the JBE245 strain that showed rapid growth and food functionality was selected and identified as Lactobacillus plantarum. This strain reached the stationary phase after 24 h fermentation at $30^{\circ}C$ with $1.5{\times}10^8$ colony forming unit (CFU)/mL of viable cells, and maintained its viability levels even after 14 days of storage. During fermentation, the ${\alpha}-glucosidase$ inhibitory activity (40.4%), total polyphenol content (583.6 mg gallic acid equivalent (GAE)/mL), and 2,2-diphenyl-l-picryl-hydrazyl hydrate (DPPH) radical scavenging activity (52%) were increased. As judged by a sensory test, the overall preference for the fermented juice (4.22) was comparable to that for the unfermented juice (4.72), indicating that fermentation does not significantly affect the sensory characteristics of apple juice. Consequently, the fermented beverage containing L. plantarum JBE245 and apple juice is a promising functional health food.

Lipids from the rhizome of Cnidium officinalis Makino (천궁으로부터 lipid 의 분리 동정)

  • Kim, Hyoung-Geun;Jeon, Hyeong-Ju;Nguyen, Trong Nguyen;Lee, Dae Young;Baek, Nam-In
    • Journal of Applied Biological Chemistry
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    • v.64 no.4
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    • pp.343-349
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    • 2021
  • The rhizomes of Cnidium officinalis were extracted in aqueous MeOH, and the concentrate was fractionated via systematic solvent fractionation to EtOAc, n-BuOH, and aqueous fractions. The repeated column chromatography of EtOAc and n-BuOH fractions using silica gel, octadecyl silica gel, and Sephadex LH-20 as stationary phase to afford five lipids. They were identified to be methyl linoleate (1), linoleic aicd (2) 6-linoleoyl-𝛼-D-glucopyranosyl 𝛽-D-fructofuranoside (3), 1-linolenoyl-3-(𝛼-D-galactopyranosyl (1→6)-𝛽-D-galactopyranosyl) glycerol (4), and 1-linoleoyl-3-(𝛼-D-galactopyranosyl (1→6)-𝛽-D-galactopyranosyl) glycerol (5) on the basis of spectroscopic data such as IR, MS, and Nuclear magnetic resonance (NMR). Compounds 1 and 3-5 were isolated for the first time from this plant in this study. The NMR data of fatty acids 1 and 2 reported in literatures are different each other. Authors identified the NMR data without ambiguity. Compound 3, a conjugate of sucrose and fatty acid, and compounds 4 and 5, digalactosyl monoglyceride, are very rarely occurred in natural source. Through the immune enhancement and anticancer activity of the reported lipid compounds, the potential as various pharmacologically active materials of Cnidium officinalis rhizome can be expected.

Comparison of Mcrobial and Physicochemical Properties between Pogi Kimchi and Mat Kimchi (포기김치와 맛김치의 미생물학적 및 이화학적 품질 특성 비교)

  • Moon, Eun Woo;Kim, Su-Yeon;Dang, Yun-Mi;Park, Boyeon;Park, Eun Jin;Song, Hye Yeon;Yang, Jisu;Yoon, So Ra;Seo, Hye-Young;Ha, Ji-Hyoung
    • Journal of the Korean Society of Food Culture
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    • v.34 no.2
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    • pp.217-223
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    • 2019
  • This study aimed to evaluate the characteristic differential between whole cabbage kimchi (pogi kimchi) and sliced cabbage kimchi (mat kimchi) during kimchi fermentation at $6^{\circ}C$. The difference of microbial and physicochemical properties was investigated until 6 weeks. For the changes in the microbial flora, both kimchi samples exhibited a continuous increase in total aerobic bacteria and lactic acid bacteria (LAB) population size up to 2 weeks followed by a stationary phase until 5 weeks. Interestingly, the number of LAB of mat kimchi was overall higher than that of pogi kimchi during kimchi fermentation. We speculate that mat kimchi has in a more advantageous growth condition than pogi kimchi for microbial growth because small kimchi cabbage size appropriately derives nutritional supply in order to increase the LAB growth. During lactic fermentation at $6^{\circ}C$, physicochemical changes in the pH, salinity, and titratable acidity was observed to be no significant differences between two types of kimchi. Furthermore the contents of organic acids such as oxalic acid, citric acid, malic acid, lactic acid, fumaric acid, and acetic acid was not significantly different (p>0.05) between both kimchi samples as well as the contents of total free amino acid.

Isolation of an Acetic Acid Bacterium Acetobacter pasteurianus CK-1 and Its Fermentation Characteristics (초산균 Acetobacter pasteurianus CK-1의 분리 및 발효 특성)

  • Bang, Kyu-Ho;Kim, Chae-Won;Kim, Chul-Ho
    • Journal of Life Science
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    • v.32 no.2
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    • pp.119-124
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    • 2022
  • To effectively isolate acetic acid bacteria for producing makgeolli vinegar, various products were researched, and Acetobacter pasteurianus CK-1, a strain that is excellent in acetic acid production, was finally isolated. The optimal growth temperature of the isolated strain was confirmed to be 30℃, and it grew well in the pH range of 5.5~6.5, with optimal growth at pH 6. A. pasteurianus CK-1 had the most active proliferation when the initial ethanol concentration in the medium was 4%, and growth was possible even at an ethanol concentration of 7%. When inoculating the isolated strain into makgeolli to induce acetic acid fermentation, the pH at the beginning of fermentation was 3.54, which was gradually lowered to 2.77 after 18 days of fermentation. The acidity was 0.75% at the beginning of fermentation and gradually started to increase from the 4th day of fermentation. The final acidity at the end of fermentation was 5.54%. In the vinegar fermented by inoculating A. pasteurianus CK-1, acetic acid content was detected to be as high as 3,575.7±48.6 mg%, and the malic acid and citric acid contents were 2,150.8±27.6 and 55.8±3.7 mg%, respectively. Further, it was confirmed that the content and ratio of the organic acids produced significantly differed depending on the type of inoculated bacterial strain. During acetic acid fermentation, the populations of yeast and A. pasteurianus CK-1 were inversely changed. In the initial stage of fermentation, yeast dominated, and after 10 days of fermentation, A. pasteurianus CK-1 slowly proliferated and reached stationary phase.

A Method for Estimating the Lung Clinical Target Volume DVH from IMRT with and without Respiratory Gating

  • J. H. Kung;P. Zygmanski;Park, N.;G. T. Y. Chen
    • Proceedings of the Korean Society of Medical Physics Conference
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    • 2002.09a
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    • pp.53-60
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    • 2002
  • Motion of lung tumors from respiration has been reported in the literature to be as large as of 1-2 cm. This motion requires an additional margin between the Clinical Target Volume (CTV) and the Planning Target Volume (PTV). While such a margin is necessary, it may not be sufficient to ensure proper delivery of Intensity Modulated Radiotherapy (IMRT) to the CTV during the simultaneous movement of the DMLC. Gated treatment has been proposed to improve normal tissues sparing as well as to ensure accurate dose coverage of the tumor volume. The following questions have not been addressed in the literature: a) what is the dose error to a target volume without gated IMRT treatment\ulcorner b) what is an acceptable gating window for such treatment. In this study, we address these questions by proposing a novel technique for calculating the 3D dose error that would result if a lung IMRT plan were delivered without gating. The method is also generalized for gated treatment with an arbitrary triggering window. IMRT plans for three patients with lung tumor were studied. The treatment plans were generated with HELIOS for delivery with 6 MV on a CL2100 Varian linear accelerator with a 26 pair MLC. A CTV to PTV margin of 1 cm was used. An IMRT planning system searches for an optimized fluence map ${\Phi}$ (x,y) for each port, which is then converted into a dynamic MLC file (DMLC). The DMLC file contains information about MLC subfield shapes and the fractional Monitor Units (MUs) to be delivered for each subfield. With a lung tumor, a CTV that executes a quasi periodic motion z(t) does not receive ${\Phi}$ (x,y), but rather an Effective Incident Fluence EIF(x,y). We numerically evaluate the EIF(x,y) from a given DMLC file by a coordinate transformation to the Target's Eye View (TEV). In the TEV coordinate system, the CTV itself is stationary, and the MLC is seen to execute a motion -z(t) that is superimposed on the DMLC motion. The resulting EIF(x,y)is inputted back into the dose calculation engine to estimate the 3D dose to a moving CTV. In this study, we model respiratory motion as a sinusoidal function with an amplitude of 10 mm in the superior-inferior direction, a period of 5 seconds, and an initial phase of zero.

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Radiolysis Assessment of $^{18}F$-FDG According to Automatic Synthesis Module (자동합성장치에 따른 $^{18}F$-FDG의 방사선분해 평가)

  • Kim, Si-Hwal;Kim, Dong-Il;Chi, Yong-Gi;Choi, Sung-Wook;Choi, Choon-Ki;Seok, Jae-Dong
    • The Korean Journal of Nuclear Medicine Technology
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    • v.16 no.1
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    • pp.8-11
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    • 2012
  • Purpose : Among quality control items, the radiochemical impurity must be below 10% of total radioactivity. In this regard, as the recently commercialized automatic synthesis module produces a large amount of 18F-FDG, radiolysis of radiopharmaceuticals is very likely to occur. Thus, this study compared the changes in radiochemical purity regarding radiolysis of $^{18}F$-FDG according to automatic synthesis module. Materials and methods : Cyclotron (PETtrace, GE Healthcare) was used to produce $^{18}F$ and automatic synthesis module (FASTlab, Tracerlab MX, GE Healthcare) was used to achieve synthesis into FDG. For radiochemical purity, Radio-TLC Scanner (AR 2000, Bioscan), GC (Gas Chromatograph, Agilent 7890A) was used to measure the content of ethanol included in $^{18}F$-FDG. Glass board applied with silica gel ($1{\times}10cm$) was used for stationary phase while a mixed liquid formed of acetonitrile and water (ratio 19:1) was used for mobile phase. High-concentration and low-concentration $^{18}F$-FDG were produced in each synthesis module and the radiochemical purity was measured every 2 hours. Results : The purity in low-concentration (below 2.59 GBq/mL) was measured as 99.26%, 98.69%, 98.25%, 98.09% in Tracerlab MX and as 99.09%, 97.83%, 96.89%, 96.62% in FASTlab according to 0, 2, 4, 6 hours changes, respectively. The purity in high-concentration (above 3.7 GBq/mL) was measured as 99.54%, 96.08%, 93.77%, 92.54% in Tracerlab MX and as 99.53%, 95.65%, 92.39%, 89.82% in FASTlab according to 0, 2, 4, 6 hours changes, respectively. Also, ethanol was not detected in GC of $^{18}F$-FDG produced in FASTlab, while 100~300 ppm ethanol was detected in Tracerlab MX. Conclusion : Whereas the change of radiochemical purity was only 3% in low-concentration $^{18}F$-FDG, the change was rapidly increased to 10% in high-concentration. Also, higher radiolysis were observed in $^{18}F$-FDG produced in FASTlab than Tracerlab MX. This is because ethanol is included in the synthesis stage of Tracerlab MX but not in the synthesis stage of FASTlab. Thus, radiolysis is influenced by radioactivity concentration than the inclusion of ethanol, which is the radioprotector. Therefore, after producing high-concentration $^{18}F$-FDG, the content must be diluted through saline to lower concentration.

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