• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.2
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• pp.181-184
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• 2003

We investigated the effect of ethanol on the production of cellulose and acetic acid fermentation by Gluconacetobacter persimmonensis KJ145. Results showed that bacterial cellulose productivity was highest when 2% ethyl alcohol was added to apple-juice medium. For acetic acid production, 7% ethyl alcohol was needed. Optimal concentration of ethyl alcohol was 5% for simultaneous production of bacterial cellulose and acetic acid. For simultaneous production of bacterial cellulose and acetic acid, optimal nitrogen source and optimal concentration were corn steep liquor and 15% (w/v), respectively Optimal culture time for simultaneous production of bacterial cellulose and acetic acid was 14 days. At the optimal condition, Cluconacetobacter persimmonenis KJ145 produced 7.55 g/L of bacterial cellulose (dry weight).

• International Journal of Advanced Culture Technology
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• v.5 no.1
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• pp.51-57
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• 2017

Digital signage is becoming part of daily life across a wide range of visual advertisements segments market used in stations, hotels, retail stores, hotels, etc. The current digital signage system used in market is generally works on limited user interactivity with static contents. In this paper, a new approach is proposed using computer vision based dynamic audience adaptive cost-effective digital signage system. The proposed design uses the Camera attached Raspberry Pi Open source platform to employ the real-time audience interaction using computer vision algorithms to extract facial features of the audience. The real-time facial features are extracted using Haar Cascade algorithm which are used for audience gender specific rendering of dynamic digital signage content. The audience facial characterization using Haar Cascade is evaluated on the FERET database with 95% accuracy for gender classification. The proposed system, developed and evaluated with male and female audiences in real-life environments camera embedded raspberry pi with good level of accuracy.

• The Journal of the Convergence on Culture Technology
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• v.4 no.1
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• pp.279-284
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• 2018

With the availability of real-time student behavioral data, personalization on education is gaining a huge traction. Data collected from massively open online courses (MOOC) has shifted the content delivery method from fixed, static to user-adopted form. Such educational content can be personalized by student's level of achivement. In this paper, we propose a service that automates the content restructuring, based on dynamic profile. With the student behavioral data, the proposed service restructures educational content by changing the order, extending and shrinking the published material. To do this, we record students' behavioral data and content information as a metadata, which will be used to generate dynamic profile.

• Journal of Life Science
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• v.13 no.4
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• pp.511-515
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• 2003

White rot fungi, Phanerochaete sp. EJ-31L, was evaluated for its ability to decolorize Remazol Black B, an azo dye that is a widespread pollutant in the wastewater of textile industry. It was observed that extent of decolorization by Phanerochaete sp. EJ-31L was dependent on the concentrations of co-carbon and nitrogen source. Effects of agitation and aeration were studied, and agitated culture at aeration condition resulted in greater extent of decolorization than static culture. Remazol Black B was readily decolorized up to 95% within 64 hr by Phanerochaete sp. EJ-31L.

• Wind and Structures
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• v.37 no.3
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• pp.245-254
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• 2023

In this paper, the wind-induced response of Jiayuguan wooden building (world cultural heritage) in Northwest China was studied. ANSYS finite element software was used to establish four kinds of building models under different working conditions and carry out modal analysis. The simulation results were compared with the field dynamic test results, obtaining the model which reflects the real vibration characteristics of the wooden tower. Time history data of fluctuating wind speed was obtained by MATLAB programming. Time domain method and ANSYS were used to analyze the wind-induced vibration response time history of Jiayuguan wooden building, obtaining the displacement time history curve of the structure. It was suggested that the wind-induced vibration coefficient of Jiayuguan wooden building is 1.76. Through analysis of the performance of the building under equivalent static wind load, the maximum displacement occurs in the three-story wall, gold column and the whole roof area, and the maximum displacement of the building is 5.39 cm. The ratio of the maximum stress value to the allowable value of wood tensile strength is 45 %. The research results can provide reference for the wind resistant design and protection of ancient buildings with similar structure to Jiayuguan wooden tower.

• KSBB Journal
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• v.22 no.2
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• pp.91-96
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• 2007

The saccharogenic liquid (SFW) obtained by the enzymatic saccharification of food wastes was used as a medium for production of bacterial cellulose (BC). The enzymatic saccharification of food wastes was carried out by the cultivation supernatant of Tricoderma inhamatum KSJ1 culture. Acetobacter xylinum KJ1 was employed for the BC production culture. Under the scaled-up aeration condition of 1.0 vvm, 5.64 g/L of BC was produced in 3 days cultivation in 50 L air circulation bioreactor using SFW medium with addition of 0.4% agar. The productivity was similar to that of 10 L air circulation bioreactor (5.84 g/L). This cultivation method with 50 L air circulation bioreactor decreasing shear stress and increasing oxygen transfer coefficient ($k_La$) was very useful in BC mass production. The physical properties, such as morphology, molecular weight, crystallinity, and tensile strength of BC produced by the static culture (A), the air circulation culture using 10 L bioreactor (B) and 50 L bioreactor (C) were investigated. The number average molecular weight of BCs produced under the different culture conditions (A-C) showed 2,578,000, 1,975,000, and 1,809,000, respectively. Tensile strength was 1.72 $kg/mm^2$, 1.19 $kg/mm^2$, and 1.18 $kg/mm^2$, respectively. All of the BCs had a form of cellulose I representing pure cellulose. The relative degree of crystallinity showed the range of 86.2$\sim$87.8%. BC production by the air circulation culture mode brought more favorable results in terms of the physical properties and its ease of scale-up. Therefore, it is expected that the new BC production method, the air circulation culture using SFW, would contribute greatly to BC-related manufacturing.

• The korean journal of orthodontics
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• v.28 no.1 s.66
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• pp.155-163
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• 1998

To evaluate the effect of a static magnetic field on the bone producing potential of MC3T3-E1 cells, the alkaline phosphatase activity was measured after the cells having been cultured under 76.4mT static magnetic field using a $SmCo_5$ magnets for 5days, 7days, 11days, 15days and 21days for each cell culture group. Also, the amount of bone nodule stained with Alizarin red S was observed. The results were as follows . The alkaline phosphatase activity of the 7, 11, and 15 days group among the experimental groups was decreased as compared with the control groups, and the decrease of alkaline phosphatase activity in the 11 days group was the most evident among them. . Any stained bone nodules of both groups had not been observed until the 11th day. The stained bone nodules in the control groups were found on the 15th day, but not in the experimental groups. The stained bone nodules were observed in both groups on the 21st day, but the control groups have more bone nodules than the experimental groups.

• Polymer(Korea)
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• v.34 no.6
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• pp.522-526
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• 2010

Bacterial cellulose is produced by the bacterium Gluconacetobacter xylinus, which forms a nanofibrous pellicle in its culture medium. We studied properties of the bacterial cellulose such as crystallinity, viscosity, morphology, and mechanical properties according to the carbon source. Static cultures of Gluconacetobacter sp. V6 were performed in three kinds of media: standard Hestrin-Schramm medium, and modified medium with either glycerol or molasses as carbon sources. Cell growth and cellulose yield were increased in the glycerol and molasses media. The culture in the glycerol medium improved the physical properties of cellulose such as crystallinity, intrinsic viscosity, and breaking stress. However, the culture in the molasses medium decreased crystallinity, crystallite size, and intrinsic viscosity of cellulose. In summary, the cellulose yield was remarkably improved in the molasses medium, but with inferior structural properties.

• Journal of the Korean Society for Precision Engineering
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• v.30 no.2
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• pp.137-142
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• 2013

The indiscriminate use of the fossil fuel has caused serious environmental pollutions such as the shortage of energy and global warming. Microalgae have being emphasized as $3^{rd}$ generation biomass which makes the carbon dioxide reduce effectively as well as produces the biofuel. Large scale production of microbial biomass by continuous culture is a quite challenging issue, because off-line optimization strategies of a microbial process utilizing a model-based scheme give rise to many difficult problems. In this paper, the static and simple control method which was able to be applied in time-variant growth environment and large scale of algae culture was studied. The significant disturbances in on-line measurement of cell density were reduced by Savitzky-Golay FIR smoothing filter. Dunaliella salina was cultivated continuously in a flat panel photobioreactor by the on-off control of the turbidostat process.

• Journal of Mechanical Science and Technology
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• v.14 no.8
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• pp.861-866
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• 2000

Flow induced shape change is important for spatial interpretation of vascular response and for understanding of mechanotransduction in a single cell. We investigated the possible shapes of endothelial cell (EC) in a mathematical model and compared these with experimental results. The linearized analytic solution from the sinusoidal wavy wall and Stokes flow was applied with the constraint of EC volume. The three dimensional structure of the human umbilical vein endothelial cell was visualized in static culture or after various durations of shear stress (20 $dyne/cm^2$ for 5, 10, 20, 40, 60, 120min). The shape ratio (width: length: height) of model agreed with that of the experimental result, which represented the drag force minimizing shape of stream-lining. EC would be streamlined in order to accommodate to the shear flow environmented by active reconstruction of cytoskeletons and membranes through a drag force the sensing mechanism.