• Journal of architectural history
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• v.32 no.5
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• pp.95-105
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• 2023

Focusing on Hwaseokjeong in Paju, the work seeks to clarify the social production of Nujeong (樓亭: Korean traditional pavilions) space, which took place over the 1960s and 70s, and its cultural-political implications. To make the effort as such meaningful, a theoretical framework based not merely on the thesis of 'social production of space' advocated by Henri Lefebvre but especially on some discussions related to 'state production of space' is prepared, according to which Hwaseokjeong is analyzed and explained. As a consequence, two principal arguments are put forward: one is that Hwaseokjeong was a social space which had been built and constructed in accordance with the unique mode of production dominating Korean society at that time called 'the state mode of production (le mode de production étatique)'; the other is that what lies beneath the denotation of a 'social space of tradition restoration' that Hwaseokjeong appears to carry is in fact the connotation of 'infiltration of anti-communist ideology'. All of these claims are once again supported both by examining Roland Barthes's semiology and mythologies and by considering Hwaseokjeong's P yeonaek (扁額: a plaque or framed text) as 'the practice of naming power through Jesa (題詞: inscribing dedications)'. While providing a succinct summary, the paper finally evaluates what 'social production of space' implies to social practices of architecture and related criticisms by taking into account the relationship between visibility and readability of space including buildings.

• Journal of Microbiology and Biotechnology
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• v.24 no.12
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• pp.1664-1672
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• 2014

In this study, a fibrous bed bioreactor (FBB) was used for $\small{D}$-lactic acid ($\small{D}$-LA) production by Sporolactobacillus inulinus Y2-8. Corn flour hydrolyzed with ${\alpha}$-amylase and saccharifying enzyme was used as a cost-efficient and nutrient-rich substrate for $\small{D}$-LA production. A maximal starch conversion rate of 93.78% was obtained. The optimum pH for $\small{D}$-LA production was determined to be 6.5. Ammonia water was determined to be an ideal neutralizing agent, which improved the $\small{D}$-LA production and purification processes. Batch fermentation and fed-batch fermentation, with both free cells and immobilized cells, were compared to highlight the advantages of FBB fermentation. In batch mode, the $\small{D}$-LA production rate of FBB fermentation was 1.62 g/l/h, which was 37.29% higher than that of free-cell fermentation, and the $\small{D}$-LA optical purities of the two fermentation methods were above 99.00%. In fe$\small{D}$-batch mode, the maximum $\small{D}$-LA concentration attained by FBB fermentation was 218.8 g/l, which was 37.67% higher than that of free-cell fermentation. Repeate$\small{D}$-batch fermentation was performed to determine the long-term performance of the FBB system, and the data indicated that the average $\small{D}$-LA production rate was 1.62 g/l/h and the average yield was 0.98 g/g. Thus, hydrolyzed corn flour fermented by S. inulinus Y2-8 in a FBB may be used for improving $\small{D}$-LA fermentation by using ammonia water as the neutralizing agent.

• Journal of the Korean Society for Nondestructive Testing
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• v.27 no.3
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• pp.231-238
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• 2007

Ultrasonic inspection of austenitic steel weldments is a truly difficult task due to complicated wave propagation phenomena such as beam skewing, splitting and distortion. In order to understand these phenomena and design proper inspection procedures, simulation is increasingly paid more attention to. This article addresses a ray tracing based approach to determine incident angle and position of optimal wave mode ultrasonic beam for flaw detection in anisotropic and inhomogeneous austenitic steel weldments. Specially, the optimal mode of ultrasonic wave wave is selected by ray tracing simulation, and an optimization approach based on ray tracing and bi-section search is proposed in order to find the ray path connecting two given points in weldments. With help of this approach, the optimal incident angle and position of ultrasonic beam can be determined for a given flaw position.

• Journal of Microbiology and Biotechnology
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• v.31 no.4
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• pp.570-583
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• 2021

Pyrococcus furiosus α-amylase can hydrolyze α-1,4 linkages in starch and related carbohydrates under hyperthermophilic condition (~ 100℃), showing great potential in a wide range of industrial applications, while its relatively low productivity from heterologous hosts has limited the industrial applications. Bacillus subtilis, a gram-positive bacterium, has been widely used in industrial production for its non-pathogenic and powerful secretory characteristics. This study was conducted to increase production of P. furiosus α-amylase in B. subtilis through three strategies. Initial experiments showed that co-expression of P. furiosus molecular chaperone peptidyl-prolyl cis-trans isomerase through genomic integration mode, using a CRISPR/Cas9 system, increased soluble amylase production. Therefore, considering that native P. furiosus α-amylase is produced within a hyperthermophilic environment and is highly thermostable, heat treatment of intact culture at 90℃ for 15 min was performed, thereby greatly increasing soluble amylase production. After optimization of the culture conditions (nitrogen source, carbon source, metal ion, temperature and pH), experiments in a 3-L fermenter yielded a soluble activity of 3,806.7 U/ml, which was 3.3- and 28.2-fold those of a control without heat treatment (1,155.1 U/ml) and an empty expression vector control (135.1 U/ml), respectively. This represents the highest P. furiosus α-amylase production reported to date and should promote innovation in the starch liquefaction process and related industrial productions. Meanwhile, heat treatment, which may promote folding of aggregated P. furiosus α-amylase into a soluble, active form through the transfer of kinetic energy, may be of general benefit when producing proteins from thermophilic archaea.

• Environmental Engineering Research
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• v.13 no.2
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• pp.93-97
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• 2008

Anaerobic co-digestion of swine manure and food waste for biogas production was performed in serum bottles at 2% volatile solids(VS) concentration and various mixing ratios of two substrates(swine manure: food waste = 100 : 0 $\sim$ 0 : 100). Through kinetic mode of surface methodology, the methane production was fitted to a Gompertz equation. The specific methane production potential of swine manure alone was lower than that of food waste. However, maximum methane production potential increased up to 1.09-1.22% as food waste composition increased up to the 80%. The maximum methane production value of food waste was 544.52 mL/g VS. It was observed that the maximum methane production potential of 601.86 mL/g VS was found at the mixing ratio of 40:60.

• Korean Journal of Environmental Agriculture
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• v.27 no.2
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• pp.145-149
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• 2008

Anaerobic co-digestion of swine manure and food waste for biogas production was performed in serum bottles at various volatile solids(VS) contents and mixing ratios of two substrates(swine manure:food waste=$100:0{\sim}0:100$). Through kinetic mode of surface methodology, the methane production was fitted to a Gompertz equation. The ultimate methane production potential of swine manure alone was lower than that of food waste regardless of VS contents. However, it was appeared that maximum methane production potentials in 80 : 20 of the mixing rate at VS 3% was enhanced at 144.7%, compared to its only swine manure. The potential increased up to 815.71 ml/g VS fed as VS concentration and food composition increased up to 3.0% and 20%, respectively. The ultimate amount of methane produced had significantly a positive relationship with that of methane yield rate. Overall, it would be strongly recommended that feeding stocks use 20% of mixing ratio of food waste based on VS 3% contents when operating the anaerobic reactor on site at $35^{\circ}C$ if not have treatment of its anaerobic waste water.

• Journal of the Korean Society for Precision Engineering
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• v.18 no.10
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• pp.161-167
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• 2001

This paper presents an approach to nonlinear engine idle controller and intake manifold absolute pressure(MAP) observer based on mean torque production model. A stable engine idle speed is important in that the unstable engine Idle mode can make engine to drooping or stall state. A sliding fuzzy controller has been designed to control engine idle speed under load disturbance. A sliding observer is also developed to estimate the intake manifold absolute pressure and compared with the actual MAP sensor value. The sliding mode observer has shown good robustness and good tracking performance. The inputs of sliding fuzzy controller are the errors of rpm and MAP. The output is a duty cycle(DC) for driving a idle speed control valve(ISCV).

• Korean Journal of Soil Science and Fertilizer
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• v.50 no.4
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• pp.293-305
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• 2017

This experiment was conducted to evaluate the effect of leachate replacement frequency on solid state anaerobic digestion (SSAD) of dairy manure using 22 L volume lab-scale digesters at mesophilic temperature ($37^{\circ}C$) in batch mode. Three different leachate replacement strategies (no replacement, once every three days, and once every nine days) were applied and three digesters per each treatment were operated for 45 days. Results showed that leachate replacement test unit every nine days resulted in 1.6 times more methane production ($53.8N{\cdot}mL\;g^{-1}{\cdot}VS$) from SSAD compared to test unit every three days ($34.0N{\cdot}mL\;g^{-1}{\cdot}VS$). No leachate replacement strategy applied group showed slightly higher methane production ($56.3N{\cdot}mL\;g^{-1}{\cdot}VS$) than every nine days replaced one. When added the methane production potential of replaced leachate itself to the methane produced from digester, leachate replacement every nine days resulted in quite similar methane production ($56.5N{\cdot}mL\;g^{-1}{\cdot}VS$) to no leachate replacement group. Even though methane production potential of replaced leachate itself added to the methane produced from digester, every three days replacement showed only $34N{\cdot}mL$ methane production per gram of volatile solids. These results suggest that farmers do not need to replace leachate during SSAD of dairy manure and sawdust mixture in order to maximize methane production. If there are any concerns with accumulation of inhibiting substances in the digester, the 9-day cycle leachate replacement is appropriate.

• Industrial Engineering and Management Systems
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• v.11 no.2
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• pp.188-195
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• 2012

This paper presents a new mode of expressing a 3D assembly work for creating a 3D CG animation without judgment by human from minimal required information. In the field of manufacturing, there are favorable movements in the utilization of 3D CAD for 3D simulation to shorten lead time for product development and pre-production. But simulating an assembly work has troubles to need huge quantity of manually input data. This paper discusses what minimal necessary information for creating 3D CG animations of assembly works is, focusing on the features of assembly works. Furthermore, a new mode of expressing a 3D assembly work is proposed as "state/change transition diagrams" (SCTD), which express arbitrary scenes in an assembly work as "state" and describe a sequential assembly work with "state" and "change", and the outline of its stepwise generation algorithm is also described. SCTD can be converted to a 3D CG animation of an assembly work without judgment by human. This paper focuses on the creating 3D CG animation of assembly works which workers use only their both hands.

• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.4
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• pp.185-193
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• 2002

With Increasing awareness towards environment-friendly and non-toxic pesticide azadirachtin obtained from neon tree (Azadirachta indica) is gaining more and more importance. Its broad-spectrum activity, Peculiar mode of action. eco-friendly and non-toxic action towards beneficial organisms has offered many advantages over chemical pesticides. All currently use commercial formulations based on azadirachtin contains azadirachtin extracted from seeds of naturally grown whole plants which is labour intensive process depending upon many uncontrollable geographical and climatic factors. Plant tissue culture can be a potential process for the pro-duction, offering consistent, stable and controlled supply of this bioactive compound, However the research on tissue culture aspects of production are in preliminary stage and requires culture and process optimization for the development of a commercially viable process. This review states the present status and future challenges of plant tissue culture for azadirachtin production.