• Journal of Dairy Science and Biotechnology
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• 제36권3호
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• pp.178-185
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• 2018

Kefir, which originates in the Caucasian mountains, is a cultured milk beverage produced by a combination of acidic and alcoholic fermentation. Kefir products are commonly used as food vehicles to deliver health-promoting materials including kefran and lactic acid bacteria to consumers. The aim of this study was to develop a freeze-dried starter culture without yeast and assess the suitability of kefir-like dairy products for the growth of lactic acid bacteria and the acidification of milk. Pasteurized whole milk (SNF 8.5%) stored at $25^{\circ}C$ was aseptically inoculated with starter cultures (0.002% w/v); it was kept at $25^{\circ}C$ until the pH attained a value of 4.6. Ten grams of the kefir-like product sample was diluted with 90 mL of 0.15% peptone water diluent in a milk dilution bottle, followed by uniform mixing for 1 min. Viable cells of Lactobacillus species were enumerated on modified-MRS agar (pH 5.2), with incubation at $37^{\circ}C$ for 48 h. Viable cells of Lactococcus species were enumerated on M17-lactose agar, with incubation at $32^{\circ}C$ for 48 h. The pH attained a value of 4.6 after fermentation for 9 h 30 min (Starter 1), 9 h 45 min (Starter 2), and 12 h (Starter 3). The viable cell count of Lactobacillus sp. and Lactococcus sp. was initially $10^5{\sim}10^6CFU/g$; it increased significantly to $10^9CFU/g$ after 12 h of incubation. During the storage of the kefir-like products at $4^{\circ}C$ for 1 4 days, the total viable cell numbers were unchanged, but the pH decreased slightly. The consistency of the kefir products increased gradually during the storage. The organoleptic properties of the kefir products fermented using the new starter culture are more desirable than those of commercial kefir. These results suggest that the newly developed starter culture without yeast could be suitable for kefir fermentation.

• 한국식품과학회지
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• 제43권1호
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• pp.45-50
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• 2011

혼합 probiotics 균과 제빵용 효모의 비를 각각 2.0:0.1, 1.5:0.1, 1.0:0.1(g/g)로 다르게 하였을 때, 다음과 같은 결과를 도출하였다. Sourdough starter의 probiotics 균의 생균수는, 발효 초기와 비교 시, 25시간 발효한 후의 생균수가 Exp 2에서는 약 130배 가까이 증가하여 다른 두 군에 비해 가장 많이 증가하였다. Sourdough starter의 pH는 전반적으로 4.1-4.4의 범위에 있어 세 군 모두 sourdough starter로 사용하기에 적당한 수치였으며, TTA는 측정결과 Exp 2군이 가장 많이 증가하였다. Sourdough starter의 발효율은 Exp 2에서 165 mL로 세 군 중에서 가장 높게 나타났다. CrumbScan의 영상분석의 결과는 Exp 2의 부피가 2,332 mL로 세군 중에서 가장 큰 것으로 나타났고, 기공의 조밀도는 유의적인 차이를 보이지 않았으며, 기공의 찌그러짐의 정도는 Exp 1에 비해 Exp 2와 Exp 3에서 1.44로 높았다. Sourdough starter와 sourdough bread의 향 성분 분석결과는, 반죽과 빵의 향 조성은 매우 다르게 나타났으나, 각각의 starter를 이용하여 sourdough bread를 제조한 후, 향성분의 변화를 살펴보면 sourdough starter에서의 주요향성분과 동일하였다. 식빵의 속질 색에 대한 관능평가에서는 전반적으로 보통의 정도로 나타났고 유의적인 차이가 없었다. Exp 2에서 기공의 상태가 가장 균일하였고, 촉감에서도 가장 부드러웠다. 그리고 향, 맛 및 전체적인 품질 평가에서도 Exp 2의 선호도가 유의적으로 높게 나타났다. 결과적으로, sourdough starter Exp 2 조성으로 제조한 sourdough bread가 모든 항목에서 가장 높게 평가되었다.

• 한국식품영양과학회지
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• 제42권9호
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• pp.1467-1474
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• 2013

묵은지 발효에 적합한 종균 발효 시스템을 개발하기 위하여 묵은지에서 분리한 유산균과 효모를 단일 또는 혼합 상태로 김치에 첨가하여 발효시킨 후 관능평가를 시행하여 묵은지 풍미에서 높은 기호도를 나타낸 유산균 ML17과 효모 MY7을 혼합 스타터로 선정하였다. 묵은지 스타터 균주는 rRNA 염기서열 분석을 통한 균주동정 결과에 따라 Lactobacillus curvatus ML17과 Saccharomyces servazzii MY7로 명명되었다. 혼합 스타터를 첨가하여 김치를 제조한 후 $10^{\circ}C$에서 7일 동안 1차 발효한 후, $-1^{\circ}C$에서 90일까지 숙성시키면서 발효 특성을 조사하였다. 대조구 김치에 비하여 스타터 김치의 pH 저하 및 산도의 증가가 빠르게 진행되어 발효 90일 후에는 1년 숙성 묵은지와 유사한 수준을 나타내었다. 물성 변화에서 김치의 경도는 전체 발효기간 동안 스타터 김치가 더 높은 경도 값을 유지하면서 아삭한 특성을 나타내었다. 미생물 균총 변화에서 총균수와 유산균수는 발효 30일에 최대 균수를 나타내었으며, 이후 감소하기 시작하여 발효 90일에는 스타터 김치의 총균수와 유산균수가 대조구 김치에 비해 낮게 나타났다. 효모수는 첨가된 스타터에 의하여 스타터 김치가 대조구 김치보다 전체 발효기간 동안 높은 균수를 유지하였다. 유산균과 효모 스타터는 발효 90일 동안 높은 점유율을 유지하여 발효 종균으로서의 우수한 특성을 나타내었다. 유기산 함량은 스타터 김치에서 lactic acid와 citric acid의 함량이 대조구 김치에 비하여 높게 나타났다. 관능평가 결과 스타터 김치는 1년 숙성 묵은지와 유사한 묵은지 맛과 높은 기호도를 나타내었다.

• 한국가금학회지
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• 제14권1호
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• pp.15-24
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• 1987

본 시험은 육계사료의 단백질수준 및 급여시기가 육계의 생산성에 미치는 효과를 구명하기 위하여 전기와 후기사료의 단백질수준을 각각 22%와 20%, 20%와 18%로 하고 전후기사료의 교체시기를 2, 4 및 6주령으로 하는 6개처리에 육계초생추 216수를 공시하여 1984년 12월 14일부터 1985년 2월 8일까지 8주간에 걸쳐 축산시험장에서 시험을 실시한 결과를 요약하면 다음과 같다. 1. 증체량은 단백질수준이 높을수록 증가하였으며, 전후기사료의 교찬시기가 빠를수록 점차 감소하였지만 사료교체시기에서 4주령과 6주령 간에는 차이가 없었다. 2. 사료섭취량은 처리간에 차이가 없었으며, 사료요구율은 전후기 사료의 단백질수준 22% 및 20%를 4주령 또는 6주령에 교체했을 때 가장 유리하였다. 3. 단위증체당 단백질요구량은 단백질수준이 높아질수록 증가하였다. 4. 육성율은 단백질수준이나 교찬시기에 의하여 영향을 받지 않았다. 5. 도체율은 처리간에 차이가 없었지만 복강지방 축적율은 단백질수준이 증가할수록, 그리고 교체시기가 늦어질수록 점차 감소하였다. 6. 수당소득은 전후기사료의 단백질수준 22% 및 20%를 4주령에 교체한 처리에서 가장 높았다. 이상의 결과를 종합해서 볼 때 육계 전기 및 후기사료의 단백질수준은 각각 22%와 20%, 교체시기는 4주령이 적합할 것으로 사료된다.

• 한국식품위생안전성학회지
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• 제30권1호
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• pp.65-73
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• 2015

The purpose of this study is to evaluate quality characteristics of the Cheonggukjang produced using rice straw-derived Bacillus cereus free starter culture (RiBS1). The Cheonggukjang was prepared in 0.1 and 1.0% inoculum concentrations of starter culture and fermented from 12 hr to 72 hr at 40 and $50^{\circ}C$. Amino-nitrogen contents after 48 hr fermentation were 559.6~590.2 mg% and 393.8~494.0 mg% at 40 and $50^{\circ}C$, respectively. Sensory evaluation showed that the Cheonggukjang fermented using RiBS1 starter for 48 hr at $50^{\circ}C$ was better than the control. And we inspected on B. cereus and biogenic amine in the Cheonggukjang produced using RiBS1 starter. As a results, B. cereus was not detected and histamine and tyramine of biogenic amine were $5.53{\pm}0.13{\sim}39.96{\pm}0.62mg/kg$. This research results showed that rice straw-derived B. cereus free starter culture (RiBS1) will be produce the Cheonggukjang with good flavour and taste.

• 동아시아식생활학회지
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• 제23권5호
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• pp.546-560
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• 2013

In this study, a natural fermentation starter formulation was developed for manufacturing Korean bread products by substituting baker's yeast with naturally fermented blueberry starters. As the incubation time of the blueberry extracts increased, the pH and total titratable acidity increased. The sweetness (brix%) of blueberry extracts containing various amounts of sugar were higher than the other sample. The result of alcoholicity for naturally fermented blueberry extracts, the fermented blueberry extract containing 20% sugar was highest. Lactic acid bacteria counts increased until the 4th day; however, it decreased from the 5th day, and viable yeast counts increased consistently until the 5th day. The volume for naturally fermented blueberry extracts increased as the incubation time increased. As the fermentation time of blueberry starters increased, the pH of bread dough decreased. The RVA analysis conveyed that wheat flour retrogradation was retarded by increasing the blueberry starter content. The weight of pan breads containing blueberry starters were higher than that of the control, while the volume, specific volume and baking loss rate were lower than those of the control. The moisture content of pan breads containing blueberry starter decreased as storage time increased. In analyzing the visible mold colony during 7 days of storage at $28^{\circ}C$, mold growth in pan breads containing the blueberry starter was retarded. The hardness of breads containing blueberry starters were significantly increased as storage time increased. The breads containing 50% naturally fermented blueberry starter have acceptable sensory properties. In conclusion, these results indicated that 50% of natural fermentation blueberry starter could be very useful as a substitute for yeast when making naturally fermented bread.

• 한국축산식품학회지
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• 제36권1호
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• pp.122-130
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• 2016

The objective of this study was to investigate the effects of mixed starter cultures isolated from kimchi on physicochemical properties, functionality and flavors of fermented ham. Physicochemical properties, microbial counts, shear force, cholesterol contents and volatile compounds of fermented ham were investigated during processing (curing and ripening time). Curing process for 7 d increased saltiness, however, decreased hunter color values (L, a, and b values). Ripening process for 21 d increased most parameters, such as saltiness, color values, weight loss, shear force and cholesterol content due to the drying process. The mixed starter culture had higher lactic acid bacteria than the commercial one. While eight volatile compounds were identified from fermented hams during curing process, total fiftyeight volatile compounds were identified from fermented hams during ripening process. The main volatile compounds were alcohols, esters and furans. However, no differences in volatile compounds were observed between two batches. Fermented hams (batch B) manufactured with probiotic starter culture (LPP) had higher sensory score in texture, color and overall acceptability than counterparts (batch A), while the opposite trend was observed in flavor. Therefore, mixed probiotic starter culture isolated from kimchi might be used as a starter culture to be able to replace with commercial starter culture (LK-30 plus) for the manufacture of fermented ham.

• Asian-Australasian Journal of Animal Sciences
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• 제33권8호
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• pp.1301-1309
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• 2020

Objective: To evaluate the use of nurse sows and post-weaning nutrition strategies for low wean weight (WW) pigs on lifetime growth and efficiency. Methods: Animals (n = 270) were assigned to one of five treatments at 28 d. Low WW pigs (<6 kg) were either weaned and offered a special dietary regime recommended for low WW pigs (WEAN) or placed on a nurse sow (NURSE) and weaned at 49 d. Normal WW pigs (9 kg) (NORM) were also weaned at 28 d. After weaning, NORM and NURSE pigs were offered either a 'high' (4 kg/pig of starter 1 diet followed by 8 kg/pig of starter 2 diet) or 'low' (8 kg/pig of starter 2 diet) starter diet allowance in a 2×2 factorial arrangement. A typical grower diet was then offered, followed by a typical finisher diet until 147 d of age. Results: NORM pigs where heavier throughout their life compared to NURSE pigs (91.4 kg vs 76.2 kg at 147 d; p<0.001). WEAN pigs were heavier at 70 d compared to NURSE pigs (23.9 kg vs 21.0 kg; p<0.001), but there was no significant difference at 147 d between NURSE and WEAN treatments. NURSE pigs had reduced feed intake throughout the finishing period (1.6 kg/d; p<0.001) compared to WEAN (2.0 kg/d) and NORM (1.9 kg/d) pigs. Feed conversion ratio (FCR) of NURSE (2.20) was lower than NORM and WEAN during the finishing period (2.40 and 2.79, respectively). Conclusion: Extended (up to 49 d) nursing for low WW pigs resulted in improved FCR during the finishing period, but no overall improvement in growth rate compared to low WW pigs weaned at 28 d and offered a specialised starter regime. Normal WW pigs where significantly heavier than low WW pigs throughout the study.

• 한국축산식품학회지
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• 제34권3호
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• pp.346-354
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• 2014

Fermented foods with probiotics having functional properties may provide beneficial effects on health. These effects are varied, depending on the type of lactic acid bacteria (LAB). Different probiotic LAB might have different functional properties. Thus, this study was performed to evaluate the quality of fermented sausages manufactured with functional starter cultures (Lactobacillus plantarum 115 and 167, and Pediococcus damnosus L12) and different fat levels, and to determine the optimum condition for the manufacture of these products. Medium-fat (~15%) fermented sausages reduced the drying time and cholesterol contents, as compared to regular-fat counterparts. In proximate analysis, the contents of moisture and protein of regular-fat products were lower than medium-fat with reduced fat content. The regular-fat products also had a lighter color and less redness, due to reduced fat content. Approximately 35 volatile compounds were identified in functional fermented sausages, and hexanal, trans-caryophyllene, and tetradecanal were the major volatile compounds. Selected mixed starter culture showed the potential possibility of replacing the commercial starter culture (LK30 plus) in flavor profiles. However, medium-fat fermented sausage containing selected mixed starter culture tended to be less acceptable than their high-fat counterparts, due to excess dry ring developed in the surface. These results indicate that the use of combinations of L. plantarum 115 and 167, and P. damnosus L12 as a starter culture, will prove useful for manufacturing the fermented sausage.

• 한국안전학회지
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• 제34권5호
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• pp.55-62
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• 2019

In this paper, we introduce a case of a fire accident during parking of a large truck that is repeatedly occurring. The shape and location of the combustion and electrical singularity commonly found in other vehicle fire accidents could limit the starter motor as the ignition section. In addition, it was possible to confirm the electrical melting singularity that could act as a cause of ignition between the start motor B terminal and the start motor enclosure. By combining the above investigations and investigations, it was possible to estimate the electric fire expressed from insulation breaking of the starter motor B terminal, and by using the renewable starter motor comparison product mounted on the fire vehicle, an experiment was performed to reproduce the ignition process from the starter motor under specific conditions. So. It is hoped that this will raise awareness about vehicle fires, which can lead to large fires or casualties, share the risks of using starter motors for regeneration, and help in the rapid and accurate investigation of similar vehicle fires in the future.