• 한국수정란이식학회지
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• 제24권4호
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• pp.275-279
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• 2009

The beneficial effect of glycerol as a cryoprotectant, especially for sperm cryopreservation, has been shown in many studies. However, glycerol is toxic to living cells, and boar sperm in particular show greater sensitivity to glycerol than sperm from other domestic animals. Amides have been studied as alternative cryoprotectants for freezing stallion sperm. Sperm frozen in methylformamide or dimethylformamide as cryoprotectants show similar motility when thawed compared with sperm frozen in glycerol. We evaluated the cryoprotective effects of dimethylformamide on boar sperm freezing. To test the effect of amides, the concentration of boar semen was adjusted to $10^9sperm/mL$, and seminal plasma was removed using Hulsen solution. After centrifugation, the pellet was diluted in modified-Modena B extender. Lactose-egg yolk (LEY) extender was used as the cooling extender. The freezing extender was madeed aaddition of the optimal amount of glycerol and amides to LEY-Glycerol-Orvus ES Paste extender, and this extender was used for the second dilution. Diluted sperm were frozen in liquid nitrogen using the 0.5 mL straw method. Sperm frozen in extender with glycerol as a cderol were compared with those frozen in extender including the different amides. Sperm were tested for motility, viability, the sperm chromatin structure assay, and normal apical ridge after thawing. The percent of motile sperm diluted in glycerol was as high as that in the stallion study (61%). Dimethylformamide showed positive effects on sperm quality and was better than glycerol. Methylformamide provided similar sperm quality as glycerol. Therefore, dimethylformamide is useful for reducing cryoinjury in boar sperm and is expected to be useful as an alternative cryoprotectant.

• 한국임상수의학회지
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• 제28권4호
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• pp.369-374
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• 2011

Coital exanthema of Thoroughbred horses had occurred firstly in Republic of Korea. This study was performed to evaluate clinical features of coital exanthema, and provide veterinary knowledge to equine practitioner as well as horse farms. The method was clinical, clinical pathology and sexual hormone, electron microscope and libido examination. Five stallions of 27, 45 broodmares of 416 showed clinical symptoms but 124 foals and a teaser did not show any abnormal appearance. Five stallion had papule, pustule, crust around a glans, body of penis, prepuce and remain decolorized spot after treatment. One laceration of glans and 3 gait disorder had been observed but it did not affect libido. Forty-five broodmares localized had papule, pustule, crust, and ulceration around perineal region, vulva, mucocutaneous junction remain decolorized spot after treatment.

• Animal cells and systems
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• 제7권2호
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• pp.119-122
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• 2003

In order to determine whether foals of move dominant mothers were harassed less by mares than those of lower-ranked mothers, harassment of foals was studied in a herd of Jeju ponies (Equus caballus) consisting of 73 mares, their 53 foals, and one stallion. Mares Harassed the foals on 71 occasions between March to September,1998. The frequency of harassment tended to decrease as foals aged, but the relationship was not significant (P = 0.079). Foals were harassed when sleeping (n = 14), grazing (n = 19), moving (n = 8), approaching a mare in the vicinity (n = 13), or play-fighting with the foal of the harassing mare (n = 17). Mares harassed foals of younger, more subordinate mares than those of older, more dominant mares (P < 0.001). We conclude that early social experience of foals is associated with the dominance rank of its mother.

• 대한수의학회지
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• 제64권1호
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• pp.8.1-8.5
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• 2024

An 18-year-old miniature Appaloosa stallion presented with 6 months of history of sanguineous crusts on medial hind limbs and discomfort of micturition. Cauliflower-like and small masses were treated with cryotherapy for 6 months, but the regrowth of masses occurred. Subsequently, local excision via laser and topical treatment with 5% 5-fluorouracil for 5 months were followed. However, the horse was euthanized 4 months later due to regrowth of the masses. The mass was diagnosed as penile papilloma with cellular atypia and Equus caballus papillomavirus type 2 (EcPV-2) DNA was detected. This is the first report of equine penile neoplasm with EcPV-2 infection in Asia.

• 공연문화연구
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• 제40호
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• pp.37-69
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• 2020

이 연구는 한국 여성영화의 흐름을 통해, 그 특징과 미학 그리고 유사성과 차이점을 살피기 위해 구상된 연구이다. 이 연구를 시행하기 위해서 시대별 대표성을 간직한 네 편의 영화를 선택해야 했다. 일제 강점기에 제작된 영화 <미몽>, 1950년대 여성주의에 대한 논쟁을 대대적으로 촉발한 <자유부인>, 여성의 황폐해진 삶과 이율배반적 남성 시각을 결합한 1990년대 영화 <은마는 오지 않는다>, 그리고 한국 페미니즘 영화의 미래 전형을 제시한 <바람난 가족>이 그 대상 작품이다. 특히 이러한 작품들은 시대별 한국 영화의 전형성과 대표성을 담보하고 있다는 점에서 주목되는 대상작이 아닐 수 없다. 이를 바탕으로 이 작품들에 나타나는 두 개의 공통 모티프를 집중적으로 조명했다. 하나는 여성의 영어 된 처지를 상징적으로 보여주는 '새장' 모티프이고, 다른 하나는 여성들이 자신의 처지를 자각하고 현재 상황을 점검하는데 필요한 '거울' 모티프이다. 한국의 여성영화는 '새장'과 '거울'의 모티프를 일정 부분 공유하고 있을 뿐만 아니라, 이러한 모티프의 연계를 통해 궁극적으로 겨냥했던 작가적 전언을 전달하는 데에도 주력해왔다.

• 대한수의학회지
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• 제42권4호
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• pp.469-473
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• 2002

This study was carried out to investigate the blood groups(Aa, Ca, Dc, Qa, Ua) and antierythrocyte antibody associated with neonatal isoerythrolysis(NI) in Thoroughbred horses. The blood groups from 1,351(35 stallions, 1,316 mares) Thoroughbred horses tested by serological procedures, and antierythrocyte antibody from 52 mares by indirect antiglobulin test. The blood groups(factor) of Aa, Ca, Dc, Qa and Ua positive were 97.1%, 100%, 91.4%, 82.9%, and 17.1% in stallions, respectively, and were negative 3.5%, 6.2%, 25.1%, 18.3%, and 77.1% in mares, respectively. These mares are considered to be at risk for production of an NI foals. The antierythrocyte antibody was not detected by this technique in all mares. These results suggest that the all breeding mares without blood groups Aa(3.5%) and Qa(18.3%) should be selected a appropriate stallion to prevent of neonatal isretythrolysis during the breeding season in Thoroughbred horses.

• 한국동물위생학회지
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• 제34권3호
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• pp.273-278
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• 2011

Sertoli cell tumor (SCT) of the testicle arises from the supporting cells within the seminiferous tubules. SCT is common in dogs, especially in cryptorchid testicles, but also has been reported in the stallion, ram, cat, and bull. Sertoli cell tumor sample was collected from 7-years male german shepherd. In this study, SCT arose from one testicle. Sample size is approximately 1.7 cm in diameter and it has a round form. In the microscopic, cells within the tumor variably resemble Sertoli cells (SCs) that normally populate the seminiferous tubules and interstitial area. There is abundant stroma of dense, mature fibrous connective tissue in SCT. In the immunohistochemical staining, cytokeratin AE1/AE3 was not expressed in the control and SCT. S-100 protein was expressed by SCs, germ cells and fibrous connective tissue of SCT. Melan A was expressed by leydig cells (LCs) of SCT. A study by using S-100 and melan A in canine SCT was almost never carried out. S-100 and melans A is considered to suggest for diagnosis and pathogenesis of canine SCTs. Inhibin-alpha and Vimentin were well known as the marekers of SCTs. Also, they were expressed by Sertoli cells and LSs of SCT in this study.

• 대한수의학회지
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• 제49권4호
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• pp.351-354
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• 2009

Diaphragmatic hernias, whether congenital or acquired (traumatic), are rarely observed in the horse. Acquired diaphragmatic hernias typically occur secondary to trauma or an increase in intraabdominal pressure due to falling, heavy exercise, or parturition. Diaphragmatic herniorrhaphy is difficult to perform in adult horses and the horses with symptomatic diaphragmatic hernias usually die. A 10- year old, 340 kg, Jeju horse (crossbred) broodmare with sudden onset of gait disorder and a moderate emaciation was examined. Findings on physical examination included conjunctivitis, dehydration, shallow breathing, dyspnea, weaken heart beat, lack of auscultatable sounds from the gastrointestinal tract, and anorexia. Rectal temperature was $38.4^{\circ}C$ and respiratory rates were moderately increased. There were slight signs of acute colic. The broodmare died one day after non-specific treatment of fluids, nutriment, antibiotics and non-steroidal anti-inflammatory drug. The cause of death was strangulation of the small intestine through a diaphragmatic hernia. The rent was about 2 cm in diameter and located in the central right part of diaphragm. Around 60 cm of small intestine was protruded into thoracic cavity through the rent. The cause of the hernia could not be ascertained. The broodmare had been pastured with many other horses, and the groom had not noticed any aggressive behavior among them. It was, however, speculated that trauma by stallion's attack may have been the cause of the diaphragmatic hernia, because the new horse may be the object of behaviors ranging from mild threats to seriously aggressive kicking, squealing, rearing, and biting.

• 한국임상수의학회지
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• 제26권3호
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• pp.282-285
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• 2009

10살의 페인트종의 말이 수컷과 비슷하게 사람과 다른 말들에게 공격적인 양상을 점차적으로 보였다. 직장검사와 초음파 검사를 통하여 오른쪽 난소의 이상을 발견하였다. 혈액치와 혈액화학치는 정상이었으나, 테스토스테론(46.00 pg/mL), 인히빈(0.856 ng/mL,), 프로게스테론(1.2 ng/mL)은 정상수치보다 증가하였다. 비대해진 난소는 diagonal paramedian approach법으로 제거하였다. 이 방법은 수술부위를 적게 절개할 수 있으며, 출혈을 최소화할 수 있다. 수술 후 합병증 없이 행동적 이상을 보이지 않았으며, 호르몬 수치도 정상 수치를 보였다. 제거한 난소는 조직검사 결과 난소 섬유종으로 진단되었다.

• 한국임상수의학회지
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• 제35권3호
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• pp.107-110
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• 2018

A 5-year-old Thoroughbred race horse was presented to Busan Korea Racing Authority equine hospital with a 3-year history of a slow-growing left rostral maxillary mass. The location and progressive growth of the mass eventually resulted in poor food prehension, quidding and mouth bit placement. The mass was solitary and hard, and covered by normal smooth oral mucosa. Radiographic examination of the maxillae showed a flocculated and mixed radiolucent lesion protruding outward and displacing the 202 and 203 teeth caudally. The 202 tooth was in normal size and the 203 tooth was hypoplastic on radiography. Under general anesthesia, a partial surgical resection of the mass was performed to minimize functional loss and facilitate prompt return to track. After surgery, there was improvement in food intake, mouth bit placement, and cosmetic appearance. Histopathological examination determined the resected maxillay mass to be an ossifying fibroma. However, there was continued growth of remnant mass in the maxilla. Equine ossifying fibroma is a rare condition and primarily affects the rostral mandible, and less commonly, the maxillae. In this case, the lesion was slow-growing, and caused cosmetic and functional impairments, including poor food intake and reduced trainability. Surgical resection was performed, but the effect of treatment was limited due to advanced size / stage of the tumor. Early dental care is suggested for horse owners to prevent belated identification and improve successful treatment of oral disorders like ossifying fibroma.