• Development and Reproduction
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• v.3 no.1
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• pp.95-100
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• 1999

Growth differentiation factor-9 (GDF-9) is a member of the transforming growth factor $\beta$ (TGF-$\beta$) superfamily. It has been known that GDF-9 is a growth factor having a crucial role in normal folliculogenesis and its expression is oocyte-specific. The present study was aimed to elucidate the expression of GDF-9 mRNA in the mouse primordial follicles as well as in the other developmental stages. The semiquantitative analysis of GDF-9 mRNA expression was conducted. Total RNA was extracted from the ICR mice ovaries at gestational day 19, postnatal day 1, day 10, day 21, and day 28, and RT-PCR was performed to measure GDF-9 and $\beta$-actin mRNA levels. Level of GDF-9 mRNA were normalized against the level of $\beta$-actin mRNA, and compared among different stages. GDF-9 mRNA was detected in all samples including the fetal ovaries that mainly consists of primordial follicles. The highest level of mRNA was observed in ovaries obtained at day 10 that mainly consists of growing follicles. The present result suggests that GDF-9 may play an important role in the early stage of folliculogenesis.

• Fisheries and Aquatic Sciences
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• v.21 no.2
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• pp.4.1-4.5
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• 2018

Insulin-like growth factors (IGFs), along with IGF-binding protein and IGF receptor, are well-known regulators in the growth and survival of vertebrates. In this study, we investigated the involvement of IGFs and protein variation during embryonic development of the olive flounder (Paralichthys olivaceus). Morphological stages were divided into six main developments as blastula, gastrula, cephalization, cranial regionalization, tail lift, and hatch. During embryonic development, protein variation was investigated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrospray ionization quadrupole time-of-flight mass spectrometry/mass spectrometry. In addition, the mechanism of signaling of IGF-I receptor was examined using immuno-blot analysis. We found marked changes in protein expression at four stages of embryonic development and identified proteins as belonging to the vitellogenin 2 family. As development progresses, expression of IGF-II, phosphotyrosine, and phospho-Akt increased, while expression of growth factor receptor-bound protein 2 (GRB2) and one of guanine-nucleotide-binding proteins (Ras) decreased. These results provide basic information on the IGF system in the embryonic development of the olive flounder.

• Korean Journal of Adult Nursing
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• v.25 no.3
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• pp.312-321
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• 2013

Purpose: The purpose of this study was to compare health promotion behavior, post-traumatic growth and quality of life according to the stages of survivorship in patients with female genital neoplasm. Methods: Data were collected from August 1st, 2011 to September 31st 2011 from 142 gynecologic cancer patients who completed treatment or were treated at an out-patient clinic. The instrument were HPLP developed by Walker, Sechrist & Pender, PTGI developed by Tedeschi & Calhoun, and Korean C-QOL. Results: Health promotion behavior scores were significantly higher in the acute survival stage than the extended survival stage. Post-traumatic growth score was higher in the acute survival stage than the extended survival stage. The quality of life scores were higher in the lasting survival stage than the extended survival stage. Conclusion: Gynecological cancer patients in the extended survival stage reported low scores of health promotion behavior, post-traumatic growth and quality of life. Intervention needed to be developed to improve health promotion behavior, post-traumatic growth and quality of life for patients with female genital neoplasm in the extended survival stage.

• Journal of Aquaculture
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• v.12 no.4
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• pp.255-266
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• 1999

The early development, growth, and morphological changes of Tiger puffer Takifugu rubripes are described on the basis of a seres of a series of reared specimens. Detailed o수 the early developmental stages are illusrtated withe special reference to morphological transformation. Egg and sperm of puffer fish Takifugu rubripes were obtained from mature adults under natural conditions, ferilized artificially and incubated in the laboratory. The incubation period of fertilizd eggs was 160 to 180 hours at a temperature of $15.1~ 18.0^{\circ}C$. Larvae were fed successively with rotifer, Artemia nauplii and artificial food for 90 days. The mean total length of newly-hatched larvae was about 2.8mm. Mouth opening occurred on the 1- day yolk-sac larvae and initial feeding was observed on th 4-5 days after hatching . The morphological transitions from the larvae to juvenile and juvenile and juvenile to young stages occurred when fish reached about 10mm in total length(about 30 days after hatching) and about 32mm in total length(about 60days after hatching) , respectively. The coefficient of variation in total length distribution increased with growth. Following the appearance of its peak at the size of about 18mm in total length, the coefficient value declined. Many changes in proportion of the body parts to total length were observed at about 9~10mm and 30~33mm in total length, corresponding to the transformations from larvae to juvenile and from juvenile to young, respectively. Also one big morphological change was observed at about 18mm in total length that divide the juvenile stage into two sub-stages.

• Korean Journal of Remote Sensing
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• v.37 no.4
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• pp.733-745
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• 2021

Although waxy corn varieties developed after the 1980s show differences depending on development stages and conditions, studies on the characteristics of waxy corn during the growth stage are rare. The subject of this study was a field survey and unmanned aerial vehicle (UAV) image acquisition of four waxy corn varieties cultivated in Idam-ri, Gammul-myeon, Goesan-gun, Korea. The study was conducted in four stages at intervals of two weeks after planting in 2019. The growth characteristics of each of the four varieties were analyzed using growth curves obtained based on field survey and UAV imagery data. The characteristics of each growth stage of the four varieties of corn, as assessed using normalized difference vegetation index (NDVI) and plant height (P.H.) values, were as follows. The growth model was identified as a model in which three-parameter logistic (3PL) curves reflect the growth characteristics of corn well. In particular, it was found that the variations in growth rate shown by P.H. and NDVI values clearly explain the differences between corn varieties. Among the four cultivars, growth and development first occurred at the early vegetative stage in Daehakchal, followed by Mibaek 2, Miheukchal, and finally Hwanggeummatchal. The variationsin P.H. and NDVI were achieved quickly and earlier in Daehakchal, followed by Mibaek 2, Hwanggeummatchal, and Miheukchal. It was confirmed that these results reflected the characteristics of the fast white-type varieties, while the black-type varieties were delayed, as in a previous study. These results reflect the resistance to lodging that affects the cultivation environment and the response characteristics to nutrients and moisture. It was confirmed that UAV accurately provides growth information that is very useful for analyzing the growth characteristics of each corn variety.

• Proceedings of the Petrological Society of Korea Conference
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• 1999.06a
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• pp.70-74
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• 1999

• Proceedings of the Korean Society of Life Science Conference
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• 2005.04a
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• pp.239-239
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• 2005

• Mycobiology
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• v.31 no.4
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• pp.205-208
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• 2003

The production of polysaccharide according to various developmental stages(mycelium growth, primordium appearance, and fruiting-body formation) in the edible mushroom Grifola frondosa was studied. The cap of the mature mushroom showed the highest amount of polysacchride. Mycelial growth and polysaccharide synthesis were optimal at pH 5 and $20^{\circ}C$. Polysaccharide synthesis was maximal after 12 days of cultivation, whereas maximum mycelial growth was shown after 18 days. Mannose, cellobiose and starch increased the level of polysaccharide as well as growth in submerged culture. Glucose and sucrose appeared to be good substrates for fruiting of Grifola frondosa.

• Proceedings of the Korean Society of Crop Science Conference
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• 2000.04a
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• pp.342-343
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• 2000

• 한국전기화학회:학술대회논문집
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• 1999.04a
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• pp.9-9
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• 1999