• Journal of Welding and Joining
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• v.15 no.3
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• pp.79-87
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• 1997

This study was performed to evaluate basic characteristics of electron beam welding process for a 9% Ni steel plate. The principal welding process parameters, such as working distance, accelerating voltage, beam current and welding speed were investigated. The AB (Arata Beam) test method was also applied to characterize beam size and energy density of the electron beam welding process. The electron beam size was found to decrease with the increase of accelerating voltage and the decrease of working distance. So, in case of high voltage (150kV), spot size and energy density of electron beam were revealed to be 0.9mm and $6.5\times10^5W/\textrm{cm}^2$ respectively. The accelerating voltage among the welding parameters was found to be the most important factor governing the penetration depth. When the accelerating voltage of electron beam was low ($\leq$90kV), beam current and welding speed did not affect on the penetration depth significantly. However, in case of high voltage ($\geq$120kV), the depth of penetration increased very sensitively with the increase of beam current and the decrease of welding speed.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.51 no.2
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• pp.265-271
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• 2015

The feeding habits of Okamejei kenojei were studied using 242 specimens collected from October 2010 to September 2011 in the coastal waters of Gadeok-do, Korea. O. kenojei was carnivorous feeder that consumed mainly shrimps, crabs, and fishes. Its diet also included small quantities of stomatods, cephalopods, amphipods. The size of O. kenojei ranged from 10.4 to 47.4 cm in total length (TL). O. kenojei showed ontogenetic dietary shift. <15.0, 15.0-19.9 cm size classes fed mainly on shrimps and amphipods. 20.0-24.9 cm size class fed mainly shrimps and crabs. Thereafter, shrimps and crabs were decreased with ontogenesis while fishes and cephalopods were increased. Increasing size, the mN/ST were constantly decreased, but mW/ST were constantly increased.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.9
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• pp.3155-3160
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• 2010

Nowadays, most car manufactures have tried to improve fuel efficiency and corrosion resistance of car body. Therefore, use of high strength steels and coated steel becomes more and more increased. In this study, spot weld characteristics according to lap sequence of sheets were analyzed using simulation method for three different steel sheet of car body which were EDDQ class coated steel with 0.7t, high strength steel 440R with 1.2t and advanced high strength steel DP 590 with 1.0t. Using simulation, weldability was evaluated by nugget size of welded zone according to nugget growth curve and welding current with respect to lap sequence of sheets. Contact resistance of each sheets contact point was used to analyze formation of nugget and optimal lap sequence was suggested.

• Journal of Microbiology and Biotechnology
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• v.14 no.3
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• pp.620-627
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• 2004

On the foundation of a database of genome sequences and protein analyses, the ability to clone a gene based on a peptide analysis is becoming more feasible and effective for identifying a specific gene and its protein product of interest. As such, the current study conducted a protein analysis using 2-D PAGE followed by MALDI- TOF and ESI-MS to identify a highly expressed gene product of C. parasitica. A distinctive and highly expressed protein spot with a molecular size of 47.2 kDa was randomly selected and MALDI-TOF MS analysis was conducted. A homology search indicated that the protein appeared to be a fungal enolase (enol). Meanwhile, multiple alignments of fungal enolases revealed a conserved amino acid sequence, from which degenerated primers were designed. A screening of the genomic $\lambda$ library of C. parasitica, using the PCR amplicon as a probe, was conducted to obtain the full-length gene, while RT-PCR was performed for the cDNA. The E. coli-expressed eno 1 exhibited enolase enzymatic activity, indicating that the cloned gene encoded the C. parasitica enolase. Moreover, ESI-MS of two of the separated peptides resolved from the protein spot on 2-D PAGE revealed sequences identical to the deduced sequences, suggesting that the cloned gene indeed encoded the resolved protein spot. Northern blot analysis indicated a consistent accumulation of an eno1 transcript during the cultivation.

• Journal of Microbiology and Biotechnology
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• v.10 no.2
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• pp.215-220
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• 2000

Abstract White spot disease (WSD), resulting in more than 90% mortality of aquacultured penaeid shrimp, has been reported off the southern and western coasts of Korea since 1993. The pafuogen of WSD has been identified as being a virion wifu an envelope around a central nucleocapsid, and with an average size of 167 nm in diameter and 375 nm in length. In the present study, an in situ hybridization technique was developed as a rapid. sensitive, and specific diagnostic assay for the WSD viros infection in shrimp. Furthermore. the pathological changes ofWSD, in shrimp experimentally infected with WSD viroses. were investigated. Using a biotinylated 643 bp probe obtained from a peR using primers specific to the rod-shaped virus of Penaeus japonicus (RV-PJ), positive signals were detected in both naturally and experimentally infected shrimps. The in situ hybridization revealed positive reactions in the nuclei of the stromal matrix cells in the lymphoid organ, epithelia of the gills, foregut. epidermis, and hematopoietic cells of the interstitial tissues, suggesting the presence of WSD virus. Tills result indicates that the in situ hybridization method can be useful for a rapid and sensitive detection of WSD viruses in shrimp.shrimp.

• Bulletin of the Korean Chemical Society
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• v.31 no.8
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• pp.2293-2298
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• 2010

Focused electrospray (FES) deposition method is presented for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. FES ion optics consists of two cylindrical focusing electrodes capped with a truncated conical electrode through which an electrospray emitter passes along the cylindrical axis. A spray of charged droplets is focused onto a sample well on a MALDI target plate under atmospheric pressure. The shape and size distributions of matrix crystals are visualized by scanning electron microscope and the mass spectra are obtained by time-of-flight mass spectrometry. Angiotensin II, bradykinin, and substance P are used as test samples, while $\alpha$-cyano-4-hydroxycinnamic acid and dihydroxybenzoic acid are employed as matrices. FES of a sample/matrix mixture produces fine crystal grains on a 1-3 mm spot and reproducibly yields the mass spectra with little shot-to-shot and spot-to-spot variations. Although FES greatly stabilizes the signals, the space charge due to matrix ions limits the detection sensitivity of peptides. To avoid the space charge problem, we adopted a dual FES/FES mode, which separately deposits matrix and sample by FES in sequence. The dual FES/FES mode reaches the detection sensitivity of 0.88 amol, enabling ultrasensitive of peptides by homogeneously depositing matrix and sample under atmospheric pressure.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.11 no.3
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• pp.1633-1649
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• 2017

In remote sensing image processing, the traditional fusion algorithm is based on the Intensity-Hue-Saturation (IHS) transformation. This method does not take into account the texture or spectrum information, spatial resolution and statistical information of the photos adequately, which leads to spectrum distortion of the image. Although traditional solutions in such application combine manifold methods, the fusion procedure is rather complicated and not suitable for practical operation. In this paper, an improved IHS transformation fusion algorithm based on the local variance weighting scheme is proposed for remote sensing images. In our proposal, firstly, the local variance of the SPOT (which comes from French "Systeme Probatoire d'Observation dela Tarre" and means "earth observing system") image is calculated by using different sliding windows. The optimal window size is then selected with the images being normalized with the optimal window local variance. Secondly, the power exponent is chosen as the mapping function, and the local variance is used to obtain the weight of the I component and match SPOT images. Then we obtain the I' component with the weight, the I component and the matched SPOT images. Finally, the final fusion image is obtained by the inverse Intensity-Hue-Saturation transformation of the I', H and S components. The proposed algorithm has been tested and compared with some other image fusion methods well known in the literature. Simulation result indicates that the proposed algorithm could obtain a superior fused image based on quantitative fusion evaluation indices.

• Fire Science and Engineering
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• v.25 no.4
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• pp.48-55
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• 2011

This paper presents the failure analysis of the rate of rise spot type heat detectors on artificially accelerated aging. The failures of heat detector turned out by two reasons. The first one is the separation of binder from plastic moulding, resulting in the leakage of air from heat chamber. The second reason is the crack of plastic. The large cracks were maybe created by these reasons, thermal expansion difference, mechanical stress, or growth of microcrack. In the sound detector, the separation and the crack were not occurred or not developed to the critical size. The glass fibers which increase the mechanical strength were added in the binder of detector 2010G. The densities of binder or plastic of each detector were similar. However, the TGA result shows that the thermal characteristics of 2005A and 2005B were not similar.

• Korean Journal of Ichthyology
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• v.32 no.3
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• pp.143-147
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• 2020

The feeding habits of ocellate spot skate (Okamejei kenojei) were studied by analysis of stomach contents. Specimens of O. kenojei (n=379) were collected in the cosatal waters five west Islands of the Korea, monthly from January to December 2019. The size of O. kenojei ranged from 5.9 to 34.5 cm in disc width (DW). O. kenojei was consumed mainly macrura (77.9%) and pisces (21.9%) in % IRI. But, brachyura, stomatopoda, cephalopods, and others showed a low ratio. The macruara feeding rate was highest in summer, whereas the proportion of pisces was the higher in winter than other seasons.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.20 no.12
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• pp.24-29
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• 2021

Recently, owing to the high demand for eco-friendly cars in the automotive industry, noise and vibrations have become major challenges. The use of laminated damping steel is increasing in response to these demands. Laminated damping steel is primarily used in sound insulation plates. The vibration energy is converted into thermal energy due to the viscoelastic resin being located between two steel sheets and being able to damp the vibrations when an external force, such as, noise or vibration is applied to the steel plate. Laminated damping steel is chiefly applied to dash panels in automotive body parts, and because of its structure, junction technology for bonding with other components is necessary. However, there has not been sufficient research conducted on junctions. In this study, regardless of the electrode shape, in the range of 4.0 ~ 8.0 kA welding current, the same welding force and welding time were applied which were 2.8 kN and 200 m/s (12 cycles) and the tensile shear load and nugget size were analyzed after the resistance spot welding between different materials of laminated damping steel with a thickness of 1.035 mm. The results show that in the range of 5 ~ 8 kA welding current, 1.035 mm laminated damping steel meets the MS181-15 standard, which is the technical standard of Hyundai-Kia Motors.