• Title/Summary/Keyword: Sporosarcina pasteurii KCTC3558

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Isolation and Characterization of Ureolytic Bacteria for Biosequestration of Strontium (스트론튬 격리화를 위한 요소 분해 박테리아의 분리 및 특성 연구)

  • Choi, Jae-Ho;Kang, Chang-Ho;Han, Sang-Hyun;Kwak, Dae Young;Oh, SooJi;So, Jae-Seong
    • KSBB Journal
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    • v.28 no.3
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    • pp.165-169
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    • 2013
  • In this study, 52 ureolytic bacterial strains were newly isolated from various environments. From these, 2 strains (TB-15 and TB-22) were selected based on their high urease activity. XRD spectra clearly showed presence of various sequestration products such as calcite and strontianite in samples. TB-22 showed 20~30% higher survivability upon Sr concentration (20 mM) than Sporosarcina pasteurii KCTC 3558. TB-15 and TB-22 showed 80~90% higher survivability at pH 6 than S. pasteurii. The results demonstrated that the 2 isolates colud be good candidates for the bioremediation of Sr contaminated sites.

Environmental Stress Response of Calcite Forming Bacteria Isolated from Concrete Pavement (콘크리트 포장도로에서 분리한 탄산칼슘형성미생물의 다양한 환경 스트레스반응)

  • Han, Sang-Hyun;Kim, Sung-Keun;Kang, Chang-Ho;Park, Joo-Young;Jeong, Jin-Hoon;So, Jae-Seong
    • KSBB Journal
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    • v.27 no.4
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    • pp.268-272
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    • 2012
  • Microbially induced calcite precipitation (MICP) has been explored for protection and consolidation of construction materials such as concrete. In this study, we isolated 54 calcite forming bacteria from concrete pavement and selected 5 isolates which showed high specific urease activity. Also response of the 5 strains against various environmental stresses was examined. BC 4 and BC 5 showed 35% and 26% viability at heat stress ($50^{\circ}C$), respectively. BC 1 and BC 4 maintained 60.4% and 70.4% viability upon osmotic stress (1 M NaCl), respectively. Among the 5 isolates BC 4 had the highest viability upon alkaline stress (pH 10).