• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.133.1-133
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• 2003

Gummy stem blight, caused by Didymella bryoniae occurs exclusively on cucurbits. This fungus has been known not to produce its pycnidium in vitro unless irradiated. Through this study, we optimized cultural conditions for mass-production of pycnidiospore by Metal Halide Lamp irradiation. In brief, the mycelial was cultured at $26^{\circ}C$ on PDA, for 2 days under the darkness, and then the plate was illuminated with MH lamp continuously for 3-4 days at $26^{\circ}C$, a great number of pycnidia was simultaneously formed. Thus produced pycnidiospores were used as immunogen. From fusions of myeloma cell (v-653) with splenocytes from immunifed mice were car ried out. And, two hybridoma cell lines that recognized the immunogen Didymella bryoniae were obtained. One Monoclonal Antibody, Db1, recognized the supernatant and the other monoclonal antibody, Db15, recognized the spore. Two clones were selected which were used to produce ascite fluid two MAb Db1 and Db15, were immunotyped and identified as IgG1 and IgG2b, respectively. Titer of MAb Db1 and MAb Db15 was measured absorbance exceeded 0.5 even at a $10^{-5}$ dilution. The MAbs reacted positively with Didymella bryoniae but none reacted with other of fungi and CMV, CGMMV Sensitivity of MAb was precise enough to detect spore concentration as low as $10^{3}$ well by indirect ELISA characterization of the MAb Db1, Db15 antigen by heat and protease treatments show that the epitope recognized by the MAb Bb1, Db15 were a glycoprotein.

• 한국응용곤충학회지
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• 제20권1호
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• pp.1-5
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• 1981

인삼 조사포닌과 인삼즙액이 인삼 근부패를 일으키는 병원균 Fusarium solani와 Erwinia carotovora의 생장과 증식 및 포자발아에 미치는 영향을 조사하여 다음과 같은 결과를 얻었다. 1. 인삼 조 Saponin의 농도가 증가됨에 따라 F. solani의 대형분생포자의 발아율은 억제 되었으며 500 ppm 이상 첨가시 현저하게 억제 되었다. 2. 토양 추출액은 초기에 F. solani의 포자 발아를 억제하는 효과가 있었으나 24시간후에는 무효화되었다. 3. 인삼 조 Saponin의 첨가 농도가 증가됨에 따라 F. solani의 포자형성량이 감소되었으며 고체배지에서 이러한 현상은 더욱 뚜렷하게 나타났다. 4. 인삼 Saponin을 첨가 했을시 균사생장량은 약간 감소되었으나 농도에 따른 감소율은 인정되지 않았다. 5. 인삼 조 사포닌은 농도가 증가 될 수록 F. solani의 Colony 형성을 억제하였으나 인삼즙액은 농도가 증가될수록 현저하게 F. solani의 Colony수를 증가시켰다. 6. 인삼 조 Saponin과 인삼즙액 모두 E. carotovora의 생장을 촉진시켰다.

• 한국응용곤충학회지
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• 제20권1호
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• pp.37-41
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• 1981

콩의 흑색뿌리썩음병을 일으키는 Cylindrocladium crotalariae은 감자한천배지와 콩의 조직을 함유한 한천 배지를 서로 다르게 Water potential를 조절하여 이 병원균을 접종한 결과 균사신장과 대형분생포자형성은 -14bars에서 좋았고 $30^{\circ}C$에서는 -100bars $25^{\circ}C$에서는 -80bars $20^{\circ}C$에서는 -64bars에서 균사신장이 중지되었으며 포자형성도 -64bars에서 중지되었다. 자낭각과 미소균핵형성은 소금을 첨가하지 않은 -1.4나 -3.0bars에서 잘 되었고 이 균에 대한 포자발아율은 -1.4bars에서 -20bars의 모든 Water potential에서 똑 같이 좋았고 -20bars에서 Water potential이 낮아지므로 발아율이 떨어지다가 -60bars에서 억제되었다.

• The Plant Pathology Journal
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• 제19권5호
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• pp.260-265
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• 2003

Gummy stem blight caused by Didymella bryoniae occurs exclusively in cucurbits. This fungus has been known not to produce its pycnidium in vitro unless irradiated. In this study, cultural conditions for the mass-production of pycnidiospore by Metal Halide (MH) lamp irradiation were maximized. The mycelia were cultured at $26^{\circ}C$ on PDA for 2 days under dark condition, and then the plate was illuminated with MH lamp continuously for 3-4 days at $26^{\circ}C$. Results show that a great number of pycnidia were simultaneously formed. The pycnidiospores produced were then used as immunogen. Fusions of myeloma cell (v-653) with splenocytes from immunized mice were carried out. Two hybridoma cell lines that recognized the immunogen D. bryoniae were obtained. One monoclonal antibody (MAb), Dbl, recognized the supernatant while another MAb, Db15, recognized the spore. Two clones were selected which were used to produce ascite fluid of the two MAb, Dbl and Db15, the immunotypes of which were identified as IgG1 and IgG2b, respectively. Titers of MAb Dbl and MAb Db15 were measured and the absorbance exceeded 0.5 even at a $10^{-5}$ dilution. The MAbs reacted positively with D. bryoniae but none reacted with other viral isolates, Cucumber mosaic virus and Cucumber green mottle mosaic virus. Sensitivity of MAb was precise enough to detect spore concentration as low as $10^{-3}$/well by indirect ELISA. Characterization of the MAbs Dbl, Db15 antigen by heat and protease treatments, which suggests that the epitope recognized by these two MAbs was glycoprotein.

• 한국버섯학회지
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• 제4권2호
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• pp.53-56
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• 2006

느타리버섯의 포자는 버섯재배 농업인의 호흡기계통질환인 알레르기를 유발한다. 느타리버섯의 무포자형성 균주는 재배농업인의 건강에 도움을 줄뿐 아니라 다른 재배사에 바이러스병 감염의 위험을 감소시킬 수 있다. 소포자 형성 느타리버섯을 육성하기 위해 무포자형성 균주의 선발 및 특성검정 결과 ASI 2069 균주가 무포자이면서 수량성이 높으나 형태적으로 상품적 가치가 없어 원형질체 재생에 의한 단핵화를 시도하여 neohaplont를 분리하였다. 원형 1호(ASI 2180) 및 무포자 느타리간의 단핵교배 (Mon-Mon)를 시도한 결과 128교잡체를 얻어 이 중 30균주를 특성 검정한 다음 자실체형성 및 담자 포자비산량 조사에 의해 소포자형성 유망 13균주를 선발하였다. 그 중에서 G192 교잡체가 소포자이면서 수량성이 좋은 가장 우량 균주로 선발되었다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2002년도 생물공학의 동향 (X)
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• pp.469-472
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• 2002

균체 생산성 실험과 chitinase 생산성 실험을 비교해 볼 때, chitinase만을 생산하는 조건 에서는 배지성분에 chitin을 첨가해 주는 것이 좋으나, 해충 방제용으로 살균력을 증진시키기 위하여 균체량과 chitinase의 생성량 및 산업적, 경제적 사용이 용이한 배지를 고려할 때에는 쌀겨와 밀기울이 첨가된 배지가 좋은 배지임을 알 수 있었다. 또한 이 배지를 이용하였을 경우 균체는 1X$10^8$ cfu/g, chitinase는 370mU/g로 생산되었으며 생물검정결과 53-64%의 탁월한 살충효과를 확인 할 수 있었다.

• 한국축산식품학회지
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• 제28권5호
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• pp.675-680
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• 2008

The physical quality and microbial safety of Korean beef jerky was evaluated at various steps during its preparation. Microbial counts in raw beef demonstrated mesophillic bacteria at 4.20 Log CFU/g, psychrotrophic bacteria at 3.85 Log CFU/g, anaerobic bacteria at 4.90 Log CFU/g, and yeast and molds at 1.92 Log CFU/g. Spore-forming bacteria and coliforms were not detected in raw beef samples. Spices and spiced meats showed similar trends in microbial counts, demonstrating minimal microbial contamination during these stages of preparation. The final beef jerky product exhibited counts of mesophillic bacteria at 1.15-1.66 Log CFU/g, psychrotrophic bacteria at 1.15-1.66 Log CFU/g, and anaerobic bacteria at 0.81-1.72 Log CFU/g. Spore-forming bacteria, yeast and molds, and coliforms were not detected in beef jerky. Significant differences from added ingredients occurred for instron textural profile analysis traits for hardness. In general, Korean beef jerky with humectant and tenderizer had lower hardness than control (without humectant and tenderizer). Also, the sample added with 0.01% protease from Streptomyces griseus had lower hardness than all samples. All samples had 0.7l to 0.72 water activities, and the color and pH were not shown in significant changes of all samples.

• Journal of Microbiology and Biotechnology
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• 제26권1호
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• pp.44-55
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• 2016

Bacillus cereus is a gram-positive, rod-shaped, spore-forming bacterium that has been isolated from contaminated fermented soybean food products and from the environment. B. cereus produces diarrheal and emetic toxins and has caused many outbreaks of foodborne diseases. In this study, we investigated whether B. amyloliquefaciens RD7-7, isolated from rice doenjang (Korean fermented soybean paste), a traditional Korean fermented soybean food, shows antimicrobial activity against B. cereus and regulates its toxin gene expression. B. amyloliquefaciens RD7-7 exhibited strong antibacterial activity against B. cereus and inhibited the expression of B. cereus toxin-related genes (groEL, nheA, nheC, and entFM). We also found that addition of water extracts of soybean and buckwheat soksungjang (Korean fermented soybean paste made in a short time) fermented with B. amyloliquefaciens RD7-7 significantly reduced the growth and toxin expression of B. cereus. These results indicate that B. amyloliquefaciens RD7-7 could be used to control B. cereus growth and toxin production in the fermented soybean food industry. Our findings also provide a basis for the development of candidate biological control agents against B. cereus to improve the safety of fermented soybean food products.

• Journal of Microbiology and Biotechnology
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• 제6권1호
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• pp.12-18
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• 1996

A chitosanase-producing bacteria was isolated on chitosan agar plate from soil samples. The strain was spore-forming gram positive bacteria, catalase positive, and rod shape. The strain was identified as Bacillus cereus. The strain did not need an inducer for the synthesis of chitosanase. Chitosanase from Bacillus sp. HW-002 was constitutive enzyme. The optimal medium for the production of the enzyme was composed of 0.5$\%$ sucrose and $1.5\%$ yeast extract-tryptone (1:1 w/w) mixture at pH 6.5. After Bacillus sp. HW-002 was cultivated at $32^{\circ}C$ for 32 h, maximal productivity was gained to be about 27, 200 U/l. Chitosanase from Bacillus sp. HW-002 was a mixed growth-linked metabolite.

• 미생물학회지
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• 제28권2호
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• pp.162-168
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• 1990

토양에서 분리한 방사균 주에서 호염기성 단백질 분해효소의 생합성과 세포분화와의 관계를 규명하고자 기균사와 포자의 형성, 그리고 단백질 분해효소의 생산에 대한 배양조건의 영향을 조사하였다. 그 결과, 기질의 농도가 단백질 분해효소, 포자, 그리고 기균사의 형성의 조절에 매우 중요하며 이것은 배양액의 pH가 산성으로 변화기 문임을 알았다. 인산염완충용액을 이용하여 배양액의 pH를 6으로부터 9로 조정하여 주었을 때 단백질 분해효소의 생성은 pH가 증가함에 따라 증가하였다. 이와 같은 결과로부터 배양액의 pH가 호염기성 단백질 분해효소 생합성의 조절에 중요한 요소로 작용한다고 판단하였다. 판단하였다.