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http://dx.doi.org/10.4014/jmb.1509.09090

Inhibition of Bacillus cereus Growth and Toxin Production by Bacillus amyloliquefaciens RD7-7 in Fermented Soybean Products  

Eom, Jeong Seon (Department of Agro-Food Resources, National Academy of Agricultural Science)
Choi, Hye Sun (Department of Agro-Food Resources, National Academy of Agricultural Science)
Publication Information
Journal of Microbiology and Biotechnology / v.26, no.1, 2016 , pp. 44-55 More about this Journal
Abstract
Bacillus cereus is a gram-positive, rod-shaped, spore-forming bacterium that has been isolated from contaminated fermented soybean food products and from the environment. B. cereus produces diarrheal and emetic toxins and has caused many outbreaks of foodborne diseases. In this study, we investigated whether B. amyloliquefaciens RD7-7, isolated from rice doenjang (Korean fermented soybean paste), a traditional Korean fermented soybean food, shows antimicrobial activity against B. cereus and regulates its toxin gene expression. B. amyloliquefaciens RD7-7 exhibited strong antibacterial activity against B. cereus and inhibited the expression of B. cereus toxin-related genes (groEL, nheA, nheC, and entFM). We also found that addition of water extracts of soybean and buckwheat soksungjang (Korean fermented soybean paste made in a short time) fermented with B. amyloliquefaciens RD7-7 significantly reduced the growth and toxin expression of B. cereus. These results indicate that B. amyloliquefaciens RD7-7 could be used to control B. cereus growth and toxin production in the fermented soybean food industry. Our findings also provide a basis for the development of candidate biological control agents against B. cereus to improve the safety of fermented soybean food products.
Keywords
Bacillus cereus; Bacillus amyloliquefaciens RD7-7; B. cereus toxin-related genes; fermented soybean food products;
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