• KSBB Journal
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• 제9권2호
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• pp.147-156
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• 1994

A study was carried out for production of a pigment : bluish purple, using a mutant Streptomyces californicus KS-89-7. The mutant was induced from Streptomyces californicus KS-89 with N-methyl-N-nitro-N-nitrosoquanidin(MNNG). It was immobilized on an inert substance made of colloidal sillica and 3.5% sodium alginate with 1 to 10 ratio. The diameter of inert bead was 2mm, and number of immobilized mutant spore was approximately $1.0{\times}10^7$/ml. It was packed in a column reactor and fermentation was conducted with a substrate made of soluble starch 1%, glycerol 1.0%, sodium glutamate 0.1%, sodium nitrate 0.05%, L-prolin 0.025% and with some trace elements. The aeration for production of the pigment was 2.5m1/min with semi-continuous fermentation. The pigment production reached at peak on 8 days of fermentation, and the mutant produced the pigment 1.8 times more than its parent strain with the maximum pigment production of $1.72g/\ell$. The pigment production continued for 24 hours of fermentation, and at the end of the fermentation the mutant produced the pigment $1.52g/\ell$.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.11-11
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• 2021

Ferns have been consumed as food in many countries for centuries. As rich sources of protein, fiber, minerals, vitamins, essential amino acids, and fatty acids, ferns provide important nutrients to humans. Eastern bracken (Pteridium aquilinum var. latiusculum (Desv.) Underw. ex A. Heller) is the most popular edible fern in South Korea where, additionally, it has long been used as an edible wild leaf vegetable. Recently, the production of eastern brackens in South Korea (2018) has reached 14,032 tons, for an annual revenue of 83.5 billion won, and even more eastern brackens are marketed if imports are taken into account as well. Most of the common ferns can be propagated using spores. However, fern farmers cultivate seedlings through traditional propagation methods, such as root pruning or rhizome division. These propagation methods exhibit limitations in forming roots and growing-points and are labor intensive. Quality seedlings of eastern bracken can be obtained through spore propagation, but the spores are fine and difficult to handle in the field. In addition, it would require appropriate environmental control. The production of synthetic seeds using encapsulation technology is easy to establish and it can be used to achieve high productivity at low cost. Synthetic seeds contain explants embedded into a seed foam, and they overcome the limitations of micropropagation and offer the possibility of using plug seedlings. Synthetic seed matrix, such as sodium alginate, has the advantages of low cost, low toxicity, and gel stability. The present study aimed to develop and produce synthetic seeds for the commercial exploitation of eastern bracken. Furthermore, we verified spore germination and the extent of gametophyte and sporophyte development achieved with our new synthetic seeds, whose production was intended to solve current problems with the handling, storage, and transportation of eastern bracken.

• 한국미생물·생명공학회지
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• 제14권3호
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• pp.259-264
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• 1986

The compositions of the four madia and their pHs for delta endotoxin production by B. thuringiensis serovar kurstaki 3a3b were examined. In the M-4 media out of the 4 media at pH8, the production of the endotoxin and spore formation were maximal. The mean generation times of the bacterium were 53.4 minutes in the M-1 media, 98 in the M-2, 132 in the M-3, and 127.5 in the M-4. The proper pHs of the media for the endotoxin production appeared to be pH 7 to 8. In the M-4 media, the lag time lasted about 5 hours.

• 한국식품영양학회지
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• 제1권2호
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• pp.102-107
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• 1988

Production of calcium lactate very useful for medical supplies of Ca-therapy was obtained by lactic acid fermentation of lactobacillus sporogenes, a spore forming lactic acid bacterium. Corn steep liquor 1%, soybean enzyme hydrolysate 3%, yeast extract powder 2% can substitute for yeast extract and peptone as nutrient sort traces in fermentation medium using 10% glucose concentration. In the calcium lactate production medium containing yeast extract powder 2%, glucose 18%, CaCO3 12%, the lactic acid fermentation was carried out at 45$^{\circ}C$ for 4days with continuous agitation of 100 rpm. As results, fermentation yield was 97.5%. The five steps such as protein coagulation, decolorizing evaporating, crystallizing, and drying were carried out to harvest calcium lactate from 10l of supernatant of fermented medium to be removed cell and CaCO3. As results, 2065.0g of white crystal calcium lactate dihyrate was recovered and a yield of 84.9% was obtained.

• 한국미생물·생명공학회지
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• 제20권5호
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• pp.527-533
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• 1992

효모탈묵용 cellulase와 xylanase 생산을 위한 조건을 조사한 결과 배지초기 pH, Tween 80, 포자접종량, 질소원의 종류 및 탄소원의 종류에 따라 효소 생산량이 크게 변하였다. Cellulase 생산을 위한 최적조건으로는 pH5.0-6.5, Tween 80 0.02, 포자현탄액($1{\times}10^{7}$/ml) 접종량 0.5-1.0, 질소원으로 면실박, 탄소원으로 옥분을 사용하였을 때 가장 효과적 이었다. 또한, xylanase 생산을 위한 최적조건으로는 pH6.5, Tween 80 0.01%, 질소원으로 corn steep liquor, 탄소원으로는 신문고지를 사용하였을 때 효과적이었으며, 이때 포자현탁액의 접종량은 cellulase 생산의 경우와 동일하였다.한편, cellulasedhk xylanase의 동시생산을 위해서는 플라스크 규모에서 질소원으로 면실박 0.5%, 탄소원으로 신문고지 1.0%와 옥분 2.0%를 첨가하였을 때 효과적이었으며, 이때 생상된 배양약의 cellulase의활성은 6.11~7.22IU/ml, xylanase의 활성은 97.7IU/ml이었다. 그러나 fermentor 규모에서는 동일 배지조성에서 효소생산이 다소 감소하였으며, 특히 xylanase 경우 배양액의 효소활성이 낮아서 이에 대한 보완이 요구된다.

• 생명과학회지
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• 제19권10호
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• pp.1478-1483
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• 2009

곡물을 소재로 혈행개선에 효과 있는 물질을 개발하기 위하여 Bacillus subtilis BK-17을 이용하여 고체 배양을 시도하였다. 고체배양에 접종하는 미생물은 영양세포보다 포자 접종이 혈전용해효소 생산에 효과적이었다. 포자 생산을 위한 배지는 SFM (0.8% nutrient broth, 0.05% yeast extract, $10^{-1}$ M $MgCl_2$, $10^{-3}$ M $FeCl_3$, $10^{-4}$ $MnCl_2$, $10^{-5}$ M dipicolic acid, pH 6.5)이 최적이었고, 포자 생성에 대한 최적 pH 와 온도는 각각 pH6과 $30^{\circ}C$였으며 포자는 배양 후 60 시간에서 가장 많은 생산을 보였다. 각종 곡물을 기질로 하였을 때 녹두가 혈전용해능이 가장 높았으며 강낭콩, 검정콩, 대두, 옥수수는 녹두의 약 90% 수준의 활성으로 높은 혈전용해능을 보였다. 반면에 보리와 밀과 같은 탄수화물 곡물은 낮은 활성을 보였다. 고체 배양한 발효 대두는 단백질과 지질 함량에 있어 발효하지 않은 대두보다 약 10% 이상의 높은 수치를 보였다. 또한 필수 아미노산과 일반 아미노산의 함량 역시 발효 대두 쪽이 약 5 - 20% 높은 것으로 보아 혈행개선기능성 뿐만 아니라 식품으로 실용 가능성이 높은 것으로 사료된다.

• 한국환경농학회지
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• 제35권1호
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• pp.72-78
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• 2016

본 곤충병원성곰팡이 B. bassiana M130의 포자를 대량생산하기 위한 방안으로 천연제오라이트 세라믹볼, 합성제오라이트 세라믹볼과 활성탄볼을 이용한 담체배양을 실시하였다. 그 중 천연제오라이트 세라믹볼 이용한 포자생산방법이 가장 우수 한 것으로 조사 되었으며, 배양 최적조건은 미강추출물(1:8, W/V) 3 mL, 전분 33 g, 포자현탁액 7 mL, pH3과 NaCl 5%로 배양하였을 때 B. bassiana M130 포자를 4.2×10⁸ conidium/mL 생산하고, 사이클론 회수방법을 통해 순수포자만 63%의 회수율을 보였다. 향후 천연제오라이트 세라믹볼을 이용한 곤충병원성 곰팡이 B. bassiana M130 포자의 대량생산이 가능하며, 온실가루이제어용 친환경살충제로 제품생산 및 원제의 대량생산을 통해 국산화 가능성을 제안하고자 한다.

• Journal of Microbiology and Biotechnology
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• 제7권4호
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• pp.278-281
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• 1997

Streptomyces sp. MCY-846 selected by in vitro cytotoxicity assay produced elaiophylin. Individual characteristics of the strains such as spore morphology, and physiological characteristics indicated that the strain is resembled to Streptomyces hygroscopicus. The time course of cell growth and antibiotic production was observed in the medium containing 0.5% trehalose and 0.5% soybean meal as carbon and nitrogen sources, respectively. The optimum production of elaiophylin was tested with different combinations of carbon and nitrogen sources and reached a maxima of $470{\mu}$/ml in the PC-II medium.

• 한국미생물·생명공학회지
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• 제25권5호
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• pp.454-458
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• 1997

To develop a yellow pigment for a food-additive, a strain producing a water-soluble yellow pigment was isolated from phylloplane of tree leaf. The strain PY123 was identified a Bacillus sp. based on morphological and biochemical characterization such as a bacillus form, mortility, spore formation, Gram positive, and catalase production. The pigment production of the strain PY123 was increased about 3 times in patato broth containing 1% sucrose and 0.1 mM CoCl$_{2}$ than in only potato broth after incubation at 30$circ$C, for 2 days. When 0.1 mM CoCl$_{2}$, was added in potato broth containing 1% sucrose at late log phase during incubation of the strain PY123, cell growth was not inhibited, and the period at maximal pigment production of the strain PY123 was about 12hrs faster than in potato broth containing 1% sucrose and 0.1 mM CoCl$_{2}$, simultaniously.

• 미생물학회지
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• 제18권2호
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• pp.51-58
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• 1980

Mutational experiments were performed to improved to improve the protease productivity of Aspergillus flavus KU 153, which is selected among the wild strains. A UV-induced mutant strain having high protease productivity was obtained by the use of the clear zone method as a simple criterion for a primary screening test. Neutral and alkaline protease activities of hte mutant strain were higher than 1.8 times, comopared with those of the parental strain, respectively, while in the case of acid protease, it was 2.7 times. The mutant strain selected was more powerful in the production of cellulase and amylase, as well s protease in wheat bran, compared with those of the parental strain. protease production of the parental strain has reached maximum level at 3 days culture, while alkaline nad neutral protease production of the mutantstrain has reached at 2 days culture. On the other hand, the mutant strain formed the spore slowly, compared with the parental strain. Column chromatography of the neutral protease on DEAE-Sephadex A-50 showed that the mutant strain was not induced the formation of another neutral protease isozyme, but induced the variation in the function of regulatory gene.