• Title/Summary/Keyword: Spore germination

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Leaf Exudates of Vicia faba and their Effects on Botrytis fabae and Some Associated Fungi

  • Migahed, Fatma F.;Nofel, Ashraf M.
    • Mycobiology
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    • v.29 no.4
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    • pp.198-204
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    • 2001
  • Analysis of leaf exudates of Vicia faba using paper chromatography to identify individual amino acids and sugars qualitatively was investigated. The results revealed that the number of identified amino acids detected in the leaf exudates of the susceptible plants was more than those of resistant plants. The results also showed an increase in the number of amino acids exuded by infected leaves, but no marked difference in sugars of infected and non infected plants. Lithium chloride application led to decrease in amino acid and sugar contents. The number of amino acids and sugars was also decreased with leaf age. Botrytis fabae and the selected fungal species(Alternaria alternata, Fusarium oxysporum and Aspergillus niger) were used to show the effect of individual amino acid and sugar on their spore germination. It was observed that all amino acids stimulated the fungal spore germination except serine which inhibited its spore germination. In case of A. alternata, spore germination was stimulated by all amino acids except serine, alanine, glutamic acid, arginine and methionine which caused the inhibition. In case of F. oxysporum, aspartic and glutamic acids inhibited spore germination but the other amino acids stimulated its spore germination. Aspartic acid and phenyl alanine inhibited the spore germination of A. niger. All the identified sugars(galactose, glucose, fructose and rhamnose) stimulated spore germination of all tested fungi.

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Effect of Fertilizers and Neem Cake Amendment in Soil on Spore Germination of Arthrobotrys dactyloides

  • Kumar, D.;Singh, K.P.;Jaiswal, R.K.
    • Mycobiology
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    • v.33 no.4
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    • pp.194-199
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    • 2005
  • Application of fertilizers such as urea, diammonium phosphate (DAP) and muriate of potash in soil adversely affected the spore germination of Arthrobotrys dactyloides. Amendment of soil with urea at the concentrations of 1.0%, 0.5% and 0.1 % completely inhibited spore germination and direct trap formation on the conidium, whereas muriate of potash delayed and reduced the spore germination even at the lowest concentration. DAP also inhibited spore germination at 1.0% concentration, while at lower concentration the percentage of spore germination was reduced. Application of neem cake at the concentration of 0.5% also inhibited spore germination after 24 h of amendment. The inhibitory effect of neem cake was reduced after 15 days of amendment, while after 30 days after amendment the inhibitory effect was completely lost and the spore germinated by direct trap as in unamended soil. Nematodes were not attracted to ungerminated spores after 24 h of amendment. After 15 days of amendment nematodes were attracted to agar blocks containing fewer germinated spores after 24 h of incubation but after 48 h of incubation large number of nematodes were attracted and trapped by the germinated spores with direct traps. After 30 days of amendment, larger number of nematodes were attracted and trapped by direct traps.

Effect of Ent-norsecurinine, an Alkaloid, on Spore Germination of Some Fungi

  • Goel, Mitul;Maurya, S.;Pandey, V.B.;Singh, V.P.;Singh, A.K.;Singh, U.P.
    • Mycobiology
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    • v.30 no.4
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    • pp.225-227
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    • 2002
  • The inhibitory activity of ent-norsecurinine alkaloid was evaluated against spore germination of some plant pathogenic fungi(Curvularia maculans, Curvularia species, C. palliscens, Colletotrichum gloeosporioides, Colletotrichum species, Afternaria solani, A. brassicae, Fusarium udum, Helminthosporium echinoclova and H. penniseti). It inhibited spore germination of all the test fungi. C. maculans, C. species, and C. palliscens were the most sensitive as complete inhibition of spore germination was observed at 1000 ppm. A. solani was not inhibited by this chemical.

Effect of Tetrahydropalmatine, an Alkaloid on Spore Germination of Some Fungi

  • Maurya, S.;Srivastava, J.S.;Jha, R.N.;Panday, V.B.;Singh, U.P.
    • Mycobiology
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    • v.29 no.3
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    • pp.142-144
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    • 2001
  • The tetrahydropahnatine alkaloid was assayed against spore germination of some saprophytic and pathogenic fungi e.g., Alternaria solani, A. brassicicola, A. brassicae, A. alternata, Erysiphe pisi, Curvularia lunata, C. pallescens, C. maculans, Curvularia species, Colletotrichum species, C. musae, Helminthosporium echinoclova, H. pennisetti, H. spiciferum, and Heterosporium sp. It inhibite spore germination of all the fungi tested. Colletotrichum spp. Curvularia lunata, Helminthosporium spiciferum and Heterosporium sp. were most sensitive as complete inhibition of spore germination was observed at very low concentration(200 ppm).

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Antifungal Activity of 2-Hydroxy 4,4'6'Trimethoxy Chalcone

  • Mishra, P.K.;Sarma, B.K.;Singhai, P.K.;Singh, U.P.
    • Mycobiology
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    • v.35 no.2
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    • pp.72-75
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    • 2007
  • Antifungal activity of 2-hydroxy 4,4'6'trimethoxy chalcone individually was tested against spore germination of ten fungi of different genera. Efficacy of the chemical was also tested against conidial germination and other growth parameters of Erysiphe pisi on excised pea leaves. 2-Hydroxy 4,4'6'trimethoxy chalcone inhibited spore germination at all the concentrations. Maximum inhibition was observed at 2000 ppm where more than 78 per cent inhibition of spore germination was observed in Ustilago cynodontis, Alternaria brassicicola, A. solani and Aspergillus flavus. It also reduced conidial germination of E. pisi significantly, when applied as pre-inoculation treatment.

Immobilization of Trichoderma harzianum ATCC52445 into Natural Matrix (천연 담체를 이용한 Trichoderma harzianum ATCC52445의 고정화)

  • 이종수;최영준복성해이준식
    • KSBB Journal
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    • v.10 no.2
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    • pp.166-169
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    • 1995
  • Spores of Trichoderma harzianum TCC 52445 for control of Rhlzocronia stem canker on potato were immobilized in various natural matrix, and germination rate and some rheological properties of the spore-matrix complex were Investigated. Germination rate of gelatin gel-spore complex and potato starch gel-spore complex were 2.8% and 2.9%, respectively, but hardness and cohesiveness of the gelstin gel-spoke complex were better than those of potato starch-spore complex. The hardness and cohesiveness were increased when the spores were immobilized in hybrid gelatin gel made by mixing several different types of natural matrix, but decreased their germination rate. Addition of corn steep liquor (1%) as spore nutrient in gelatin gel-spore complex was helpful for increasing the germination rate.

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Antifungal Activity of a Phytoterpenoid (AOS-A) Isolated from Artabotrytis odoratissimus on Spore Germination of Some Fungi

  • Singh D.K.;Basha S. Ameer;Sarma B.K.;Pandey V.B.;Srivastava J.S.
    • Mycobiology
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    • v.34 no.3
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    • pp.120-123
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    • 2006
  • Phytoterpenoid isolated from Artabotrytis odoratissimus inhibited spore germination of some plant pathogenic as well as saprophytic fungi e.g. Alternaria alternata, A. solani, Cercospora sp., Curvularia maculans, C. pennisetti, Fusarium udum, Helminthosporium echinochlova, H. frumentacie, H. penniseti and Ustilago cynodontis. In Curvularia maculans and H. frumentacie, spore germination was completely inhibited at 2000 ppm. However, Curvularia maculans and C. pennisetti showed considerable sensitivity to this chemical even at 500 ppm.

Antifungal Activity of an Alkaloid Allosecurinine against Some Fungi

  • Singh, A.K.;Pandey, M.B.;Singh, U.P.
    • Mycobiology
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    • v.35 no.2
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    • pp.62-64
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    • 2007
  • An allosecurinine alkaloid was assayed against spore germination of some saprophytic and pathogenic fungi e.g., Alternaria alternata, A. solani, A. brassicicola, A. brassicae, Curvularia lunata, C. pallescens, C. maculans, Curvularia species, Colletotrichum species, C. musae, C. gloeosporioides, Erysiphe pisi, Fusarium udum, Helminthosporium echinoclova, H. pennisetti, H. spiciferum, and Heterosporium sp. It inhibited mild spore germination of all the fungi tested. Curvularia lunata, Curvularia sp., Collectotrichum sp., C. musae and Heterosporium sp. were most sensitive as complete inhibition of spore germination was observed at very low concentrations.

Effect of 1-corydalmine, an Alkaloid Isolated from Corydalis chaerophylla Roots on Spore Germination of Some Fungi

  • Basha, S. Ameer;Jha, R.N.;Pandey, V.B.;Singh, U.P.
    • Mycobiology
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    • v.35 no.2
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    • pp.69-71
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    • 2007
  • 1-Corydalmine, an alkaloid isolated from roots of Corydalis ehaerophylla inhibited spore germination of some plant pathogenic as well as saprophytic fungi e.g. Alternaria brassicae, A. brassicieola, A. solani, Curvularia lunata, C. maculans, C. sp., C. pallseens, Erysiphe pisi, Fusarium udum, Helminthosporium species, H. penniseti and a Heterosporium species. 1-Corydalmine significantly inhibited spore germination of all the fungi at 100 to 1500 ppm. It was effective against all the fungi at 1500 ppm. C. lunata was highly sensitive to this chemical even at 250 ppm.

Physical Factors in Relation to Conidial Germination in Alternaria porri, the Incitant of Purple Blotch of Onion (양파검은무늬병균의 포자발아에 마치는 물리적 요인)

  • Gupta, R.B.L.;Pathak, V.N.;Verma, O.P.
    • Korean journal of applied entomology
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    • v.24 no.3 s.64
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    • pp.135-139
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    • 1985
  • Influence of temperature, relative humidity, spore washing and spore drying on conidial germination of Alternaria porri(Ell.) Cif. was studied. Maximum conidial germination occurred at 100% relative humidity prevailing for 6 hours or more at $25^{\circ}C$. Conidial germination decreased with increase in number of spore washings. Drying of conidia for more than half an hour caused significant decrease in germination. In all the experiments, conidial germinatio increased with increase in incubation period.

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