• Analytical Science and Technology
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• v.32 no.4
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• pp.155-162
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• 2019

Analysis techniques using DNA profiling are widely used in various fields including forensic science and new technologies such as the Direct PCR amplification method are being developed continuously in order to acquire the DNA profiles efficiently. However, it has a limits such as non-specific amplification according to the quality of crime scene evidence samples. Especially, split peaks caused by excessive DNA samples are one of the important factors that could cause the debate to allow researchers to interpret the DNA profile results. In this study, we confirmed the occurrence rate of split peaks in each STR (short tandem repeats) locus of the $GlobalFiler^{TM}$ kit and investigated the possibility of improving the split peaks using several PCR additives such as DMSO (dimethylsulfoxide), $MgCl_2$, Betaine and Tween-20. As a result, we could make three groups according to the occurrence rate of split peaks in Direct PCR and it was confirmed that the ratio of split peaks could be reduced by DMSO (87.4 %), $MgCl_2$ (84.5 %) and Betaine (86.1 %), respectively. These results indicate that PCR additives such as DMSO, $MgCl_2$ and Betaine can be improve the split peaks in Direct PCR and thereby facilitate subsequently a successful DNA profile results.

• Journal of The Korean Society of Grassland and Forage Science
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• v.23 no.1
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• pp.37-42
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• 2003

This study was conducted at a volcanic ash soil in the Experimental Farm of Cheju national university from May 1, 2000 to August 25, 2000 to determine the optimum frequency of split N application for. forage production of Jeju Italian millet(Setaria italica Beauvis). N .rate was applied with 200kg N/ha, and frequencies of the split application were 1. 2, 3, 4 and f times. Days to heading was 87 days in the N applied plot all at once, was delayed to 93 days at the five times split-applied plot. Plant height was the greatest (143cm) at the four times split-applied plot, but above o. below that was short. Leaf length, number of leaves and nodes were a similar tendency to plant height. SPAD(Soil Plant Analysis Development) reading values rose 34.3∼36.2 as N was split-applied from one to five times. Fresh forage, dry matter, crude Protein and TDN yield at the H split-applied to four times increased 33.08∼5l.50MT/ha, 9.94∼13.36MT/ha, 0.93∼1.70MT/ha and 5.06∼7.28MT/ha, respectively, but at the five tines split-applied plot decreased to 49.33MT/ha, 12.69MT/ha, 1.65MT/ha and 6.98 MT/ha, respectively. As the increasing of N split-applied. crude protein, crude fat NFE and TDN content increased 9.4∼13.0％, 1.5∼l.9％, 44.5∼45.5% and 50.9∼55.0％, respectively, whereas crude fiber and crude ash content decreased 35.3∼31.6% and 9.3∼8.3, respectively.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.1
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• pp.49-53
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• 1998

Direct-seeding has major advantages such as labor and cost saving by eliminating preparation of seed bed and transplanting. But, it required increased input of fertilizers and pesticides because of the extended paddy period. Direct seeding in wet paddy (DSWP) gives faster growth and more uniform seedling emergence than direct-seeding in dry paddy. This research had an objective to develop an efficient N management practices for DSWP with split application of N fertilizer. A paddy field experiment was conducted to evaluate effects of starter N and N-topdressing which was delayed N application until 5-leaf stage, with comparison to transplanting (TP). Total amount of N application were two levels; 110kg and 77kg/ha. The N applications were split four times during rice growth stages; starter, topdressing at 5-leaf stage, top dressing at tillering stage, and topdressing at panicle initiation stage. DSWP had more tillers/$m^2$ than TP, but with the delayed heading. The DSWP plots which received N-topdressing at 5-leaf stage without starter N had higher leaf area index (LAI) and leaf greenness than the TP plot. Also, these DSWP plots had high leaf-N concentration at the heading stage, as calculated from leaf chlorophyll meter readings. Rice yield in DSWP with N-topdressing at 5-leaf stage was significantly higher than that in TP and in DSWP with starter N. Energy and N use efficiency were improved in DSWP with N-topdressing at 5-leaf stage. But, there were no significant differences in grain yield between the two levels of total amounts of N applications, 77kg and 110kg/ha. We concluded that starter N could not be used effectively by rice seedlings, but topdressing N at 5-leaf stage was an efficient N management for rice growth and yield in DSWP system.

• Journal of the Korean Solar Energy Society
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• v.34 no.6
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• pp.39-47
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• 2014

Daylighting has a great effect on people inside the room. It is also closely related to the lighting energy consumption. Daylight factor(DF) is a very important index for evaluation of the daylighting in overcast sky. The objective of this study is to verify the suitability of the Tregenza's Modified Split Flux formula for the calculation of the daylight factor. We compared the daylight factors calculated by two methods; one by a measurement with 1/5 scale model and the other by the Daysim program. We used variables for verification as window wall ratios(WWR) and angles of the sky visible. As a result, daylight factor calculated by Modified Split Flux is similar to the measurement when more daylight enters the inside. And error is significantly increased when the angle of the sky visible is $50^{\circ}$.

• Korean Journal of Medicinal Crop Science
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• v.8 no.1
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• pp.69-73
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• 2000

To investigate proper method of nitrogen application, six levels of nitrogen fertilizer and five different split applications were tested in Liriope platyphylla. Leaf length and content of total nitrogen in plant increased with the increase of nitrogen application level from zero to 360kg per ha. Number and weight of tuber increased with the increase of nitrogen level and tuber production reached to highest level of 4.44M/T per ha with the level of 220kg nitrogen. And then, tuber production decreased with the addition of extra nitrogen fertilizer to level of 360kg per ha. In the research of proper nitrogen split application, tuber yield (3.52M/T per ha) was increased by 18% at the combination of 40% basal application and three times of top dressing, than that of 100% basal application.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.225-225
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• 2017

This study is to investigate the effect of split application and adapted fertilization application cultivation in stable medicinal herbs production of Acorus graminens S. And it accomplished one's purpose to GAP guide manual established standard method of cultivation technology, contribute to the enlargement of farm's income. Method of fertilization application were conducted under five condition compose to standard application(N-P-K-Compost: fermented mixed oil cake fertilizer applied at $4-2-6-300kg.10a^{-1}$, twofold application(N-P-K-Compost applied at $8-4-12-600kg.10a^{-1}$) cultivation. Compost (fermented mixed oil cake fertilizer) and fused phosphate applied at 100% of basal fertilizer in April 14. The split application level of standard application and twofold application(N-K) applied at 100%, 50%, 40% of basal fertilizer in April 14, 50% and 40% of top dressing were three times application (N-K) in June 1, July 15, August 15, 25% and 20% respectively. Transplanting with Naju varieties in May 12 by growing pot seedling for 40 days in 2015. Planting density were spaced 30 cm apart in rows 30 cm apart with black vinyl mulching on open cultivation. Plot design randomized block 3 repetition. Therefore, growth characteristics by 50% and 40% of top dressing were three times application (N-K) compared to standard application and twofold application (N-K) applied at 100% basal fertilizer, aerial part as a result were so many such amount of growth as number of leaf per plant, etc., Length of diameter and main root of subterranean part growth increment rising highest. Dried roots yields treated standard fertilizer 100% application($250kg.10a^{-1}$) compared to 100% from twofold application (N-K) applied at 50% basal fertilizer, 50% and 40% of top dressing were three times application increased by 16%, 20%. respectively.

• Korean Journal of Soil Science and Fertilizer
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• v.8 no.2
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• pp.81-87
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• 1975

A pot experiment was conducted to study the effect of two methods of applying potassium fertilizer to rice. One basic application was compared with the split of same total quantity into four applications as follows: 15 days after transplanting (40%), Ear formation stage (30%), 13 days before heading (20%) and 7 days after heading (10%) Each of these two treatments was carried out on both untreated soil and soil to which wollastonite and lime material had been added. The number of ripened grains or the 1,000 grain weight was increased by application of potassium to untreated soil. However, on soil treated with lime and wollastonite only the number of total grains was increased by potassium application. In both cases, split application of the potassium was more affective than a single basic application. No significant increase in yield was obtained from a single basic application of potassium. However, split application of the same total quantity of potassium did give a significant increase in yield. A negative correlation was found between the content of $K_2O$ and that of other nutrients in the rice plant at two stages of growth. Significant negative correlation was obtained between the content of $K_2O$ and magnesium, phosphorus and nitrogen at ear formation stage, and between the content of $K_2O$ and calcium and silicate including magnesium, phosphorus and nitrogen at heading stage. This result also indicated that the depression of uptake of phosphorus and magnesium at ear formation stage and that of calcium and silicate at heading stage were decreased by potassium split application. However, the degression of uptake of nitrogen at ear formation stage and that of magnesium at heading stage were increased by potassium split application.

• Journal of Korean Society of Steel Construction
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• v.24 no.5
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• pp.511-520
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• 2012

The double split Tee connection, a type of full strength-partially restrained connection, has adequate flexural strength according to the changes in the thickness of the T-stub flange and the gauge distance of the high-strength bolts. Moreover, the double split Tee connection is designed and constructed with seismic connections that have enough ductility capacity applicable to ordinary moment frame and special moment frame by grade of steel, size of beam and column and geometric connection shape. However, such a domestic research and a proposal of a suitable design formula about the double split Tee connection are insufficient. Thus, many experimental and analytical studies are in need for the domestic application of the double split Tee connection. Therefore, this study aimed to examine and suggest feasibility of a design formula of the double split Tee connection of FEMA.

• Korean Journal of Soil Science and Fertilizer
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• v.7 no.2
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• pp.107-112
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• 1974

In order to establish a rational application method of potash to paddy rice(Oryza sativa L. Jinheung, a Japonica variety) field and pot experiments on split application of two rates (6kg and 12kg $K_2O$ per 10a) of potash at a certain critical growing stage have conducted in 1973 and the results were as follows: 1. At the treatment where a rate of 6kg $K_2O$ per 10a(lower dose) of potash was supplied in two split doses, a half at transplanting time and the other half at young panicle formation stage, the yield of paddy rice appeatred to be low due to less number of panicles per hill. (Field experiment) 2. The treatment where potash supply ceased at transplanting time or at effective tillering stage but supplied at the other two stages (including panicle initiation stage) have failed to increase K content in the flag leaf and have shown low maturity and low thousand grain weight, which resulted in low paddy rice yield. (pot experiment) 3. From above two facts, it is concluded that the application of potash at transplanting time is an important practice for the high yielding of paddy rice. 4. The application of lime seemed to stimulate later growth of paddy depressing the unnecessary increase of poor miserable tillers in early stages of growth.

• Journal of the Korean Mathematical Society
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• v.58 no.4
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• pp.1019-1033
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• 2021

Let 𝚪 be a Gorenstein coalgebra over a filed k. We introduce the Gorenstein transpose via a minimal Gorenstein injective copresentation of a quasi-finite 𝚪-comodule, and obtain a relation between a Gorenstein transpose of a quasi-finite comodule and a transpose of the same comodule. As an application, we obtain that the almost split sequences are constructed in terms of Gorenstein transpose.