• Fibers and Polymers
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• v.6 no.1
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• pp.72-78
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• 2005

The impact of fiber friction, yarn twist, and splicing air pressure on mechanical and structural properties of spliced portion have been reported in the present paper. The mechanical properties include the tensile and bending related properties and, in the structural properties, the diameter and packing density of the splices are studied. A three variable three level facto­rial design approach proposed by Box and Behnken has been used to design the experiment. The results indicate that there is a strong correlation between retained spliced strength (RSS) and retained splice elongation (RSE) with all the experimental variables. It has been observed that RSS increases with the increase in splice air pressure and after certain level it drops, whereas it consistently increases with the increase in yarn twist. The RSE increases with the increase in both fiber friction and yarn twist. It has also been observed that the yarn twist and splicing air pressure have significant influence on splice diameter, percent increase in diameter and retained packing coefficient, but the fiber friction has negligible influence on these parame­ters. Yarn twist and splicing air pressure has a strong correlation with splice flexural rigidity, where as poor correlation with retained flexural rigidity.

• The Korean Journal of Physiology and Pharmacology
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• v.11 no.3
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• pp.135-138
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• 2007

Hypertonicity imposes a great deal of stress to cells since it causes rise in cellular ionic strength, which can be reduced by the accumulation of compatible osmolytes. TonEBP plays a central role in the cellular accumulation of compatible osmolytes via transcriptional stimulation of membrane transporters and aldose reductase. Alternatively spliced forms of TonEBP mRNA have previously been reported and two of them showed different transcriptional activity. In the present study, isoform-specific antibodies were produced to confirm the translation of the spliced mRNA to protein. TonEBP was immunoprecipitated by using anti-TonEBP antibody and then immunoblotted using anti-TonEBP or isoform specific antibodies to find out the expression profile of TonEBP isoforms in basal or stimulated condition. From these results, we conclude that all TonEBP isoforms are expressed in mammalian cells and their expression patterns are not same in every cells.

• Genomics & Informatics
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• v.3 no.4
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• pp.172-174
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• 2005

Recent anti-cancer approaches have been based to target tumor-specifically associated and/or causative molecules such as RNAs or proteins. As this specifically targeted anti-cancer modulator, we have previously described a novel human cancer gene therapeutic agent that is Tetrahymena group I intron-based trans-splicing ribozyme which can reprogram and replace human telomerase reverse transcriptase (hTERT) RNA to selectively induce tumor-specific cytotoxicity in cancer cells expressing the target RNA. Moreover, the specific ribozyme has been shown to efficiently retard tumor tissues in xenograft mice which had been inoculated with hTERT-expressing human cancer cells. In this study, we assessed specificity of trans-splicing reaction in cells to evaluate the therapeutic feasibility of the specific ribozyme. In order to analyze the trans-spliced products by the specific ribozyme in hTERT-positive cells, RT, 5'-end RACE-PCR, and sequencing reactions of the spliced RNAs were employed. Then, whole analyzed products resulted from reactions only with the hTERT RNA. This study suggested that the developed ribozyme perform highly specific RNA replacement of the target RNA in cells, hence trans-splicing ribozyme will be one of specific agents for genetic approach to revert cancer.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2003.09a
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• pp.263-270
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• 2003

Before the implementation of the 1992 seismic design provisions in Korea, longitudinal steels of RC bridge piers were practically lap-spliced in the plastic hinge region. Experimental investigation was made to evaluate the seismic performance of RC bridge pier specimens in a flexure/shear mode. Six circular test specimens in an aspect ratio of 2.5 (600mm in diameter) were made with test parameters confinement ratio, lap splices, and retrofit FRP materials. They were damaged under a series of artificial earthquakes with 0.22g PGA, being compatible in Korean peninsula, through the pseudo-dynamic test. Probable damages were assessed by the Park and Ang damage index. Approximate 0.1 and 0.3 damage indices were obtained for RC specimens without lap splice and with lap splice, respectively. Directly after the pseudo-dynamic test, damaged test columns were laterally actuated under inelastic reversal cyclic loadings simultaneously under a constant axial load. Through curvature measurements, residual seismic performance was evaluated for test specimens. Test results show that RC pier specimens with lap-spliced appeared to fail at low ductility, but significant improvement was obtained for the ductility of these specimens if externally wrapped with FRP.

• Structural Engineering and Mechanics
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• v.19 no.3
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• pp.337-346
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• 2005

The paper reports on a study of bond strength between reduced-water-content concrete and tensile reinforcement in spliced mode. Three different diameters (12, 16 and 22 mm) of tensile steel were spliced in the constant moment zone, where there were two bars of same size in tension. For each diameter of reinforcement, a total of nine beams ($1900{\times}270{\times}180mm$) were tested, of which three beams were with no axial force (positive bending) and the other six beams were with axial force (combined bending). The splice length was selected so that bars would fail in bond, splitting the concrete cover in the splice region, before reaching the yield point. It was found that there was a considerable size effect in the experimental results, i.e., as the diameter of the reinforcement reduced the bond strength and the deflection recorded at the midspan increased significantly, whilst the stiffness of the beams reduced. It was also found for all reinforcement sizes that higher bond strength and stiffness were obtained for beams tested in combined bending than that of the beams tested in positive bending only.

• Advances in materials Research
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• v.8 no.1
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• pp.25-36
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• 2019

The utilization of fibre in concrete production not only solves the problem of disposing this solid waste but helps conserve natural resources. This study investigated the effect of waste aluminum shavings on bond strength of laterized concrete. Laterized concrete spliced beams of $150{\times}250{\times}2150mm$ and $175{\times}275{\times}2300mm$ were prepared. Fifteen specimens with 16 mm and 20 mm were cast with the addition of aluminium shavings at varying percentages of 1vol%, 1.5vol% and 2vol%; another ten specimens with 16 mm and 20 mm diameter bars at 0% of aluminium shavings were cast as control. Concrete cubes of number were prepared, three taken for each set of various percentages of aluminium shavings were used to determine the concrete strength. It was observed from the analysis that the compressive strength decreased as the percentage of aluminium shavings increased, while the aluminium shavings increased the bond between concrete and steel. However, for normal concrete there was an increase in bond resistance with increase in aluminium shavings. The bond resistance of 16 mm was found to be higher than that of 20 mm in all the specimens tested.

• Journal of the Earthquake Engineering Society of Korea
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• v.13 no.6
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• pp.87-98
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• 2009

It is well known that reinforcement details in the plastic hinge region of bridge piers give the most important effects on the seismic performance of bridges, from investigations of bridge failures in many earthquake events and in laboratory tests. Longitudinal reinforcement details give larger effects than lateral reinforcement details do. The lap-spliced longitudinal steel shows slip during earthquake events, which results in low ductility and inadequate seismic performance. However, before the issue of the earthquake design code, a considerable number of bridge piers were constructed with lap-spliced longitudinal steel in the plastic hinge region. Therefore, a large amount of research has been conducted on the seismic performance and retrofit of circular and rectangular shaped bridge columns with lap-spliced longitudinal steel. However, research on wall type piers is very limited. This paper investigates the seismic performance of a pier wall by a quasi-static test in the weak axis direction and proposes a retrofit method. From the test with variables being the longitudinal steel detail and the transverse steel amount, it is shown that the currently used definition of yield displacement is not adequate. Therefore a new definition of yield displacement for the ductility investigation for a pier wall is proposed. In addition, a retrofit method by steel plates and bolts is proposed to improve ductility, and test results show that slip of the longitudinal steel is prevented by up to a considerably large displacement.

• Journal of the Earthquake Engineering Society of Korea
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• v.16 no.5
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• pp.41-53
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• 2012

The objective of this study is to evaluate the seismic capacity of RC piers with small aspect ratios. Test specimens were selected from the prototype piers among existing national roadway bridges which are expected to fail in shear and/or complex shear-flexural mode. Two groups of full scale RC pier models were constructed with aspect ratios of 2.25 and 2.67. Quasi-static tests have been implemented to investigate the failure behavior of the RC piers in terms of the lap-spliced longitudinal reinforcing steel and the aspect ratio. It is confirmed that regarding its shear-flexural behavior, the pier is very sensitive to the aspect ratio or details. In the case of a test pier with highly lap-spliced longitudinal bars, the bond failure of lap-splice steels was the dominant cause of failure before the occurrence of flexure or shear-flexural failure, despite a slight change in the aspect ratio. Finally, based on the test results and analysis, this paper proposes formulas for the yielding and ultimate displacements of circular reinforced concrete bridge piers without seismic details. These formulas will be useful for the investigation and upgrade of the seismic capacity of bridge piers without seismic details.

• Journal of the Korea Concrete Institute
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• v.20 no.4
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• pp.451-458
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• 2008

The near-fault ground motion (NFGM) is characterized by a single long period velocity pulse of large magnitude. NFGM's have been observed in recent strong earthquakes, Izmit Turkey (1999), Kobe Japan (1995), Northridge USA (1994), etc. These strong earthquakes have caused considerable damage to infrastructures because the epicenter was close to the urban area, called as NFGM. Extensive research for the near-fault ground motion (NFGM) have been carried out in strong seismic region, but limited research have been done for NFGM in low or moderate seismic regions because of very few records. The purpose of this study is to investigate and analyze the effect of near-fault ground motions on reinforced concrete (RC) bridge piers with lap-spliced longitudinal reinforcing steels. The seismic performance of four RC bridge piers under near-fault ground motions was investigated on the shake table. In addition, a RC bridge pier is subjected to pseudo-dynamic loadings. Test results showed that large residual displacements were observed in RC bridge piers under NFGM. RC specimens on the shake table failed at relatively low displacement ductility, compared with the displacement ductility of RC bridge pier subjected to pseudo-dynamic loadings.

• Journal of the Korea institute for structural maintenance and inspection
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• v.25 no.6
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• pp.76-83
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• 2021

This study examines the bending behavior of lap-spliced ultra-high-strength fiber-reinforced concrete members and evaluates the safety of the design codes for ultra-high-strength fiber-reinforced concrete structures. An experiment on a total of six beams was performed. The main variables were the fiber-inclusion and the lap-spliced length at the center of the beams. The steel fibers in a volume fraction of 2% were used, and the lap-splice lengths were determined to be 8db and 16db. As a result of the test, the specimens not reinforced with fiber lost abrupt load-bearing capacity at the lap region and did not experience yielding of the reinforcing bar. In the case of fiber-reinforced concrete, if a lap-splice length of 16db is secured, the yielding of the main reinforcing bar can be experienced, and appropriate flexural strength can be expressed. Based on the experimental results of this study, as a result of reviewing the lap-splice length calculation formulas of the current design standards and the ultra-high-strength concrete structural design recommendations, it was found that all of them were evaluated conservatively.