• Korean Journal of Microbiology
• /
• v.27 no.1
• /
• pp.1-9
• /
• 1989

The locations of 5' ends as well as the splicing pattern of viral poly A(-) 19S RNA from monkey cells infected with SV40 were determined by a modification of primer extension method. The 5' end of this RNA mapped at the major cap site at nucleotide residue 325, used most frequently by SV40 late RNAs. The intron from nt.373 to nt.558 was removed as the ordinary cytoplasmic poly A(+) 19S RNA. The 3'end of this RNA was very heterogeneous and distributed over 1 kb upstream of polyadenylation site, as determined by S1 nuclease mapping. The reason for this normally initiated and spliced RNA to accumulate in the nucleus was investigated. In order to test whether the presence of unused 3' splice region on this RNA caused such subcellular distribution, cells were transfected with SV40 mutant KNA containing deletion around 3' splice site. The RNA deleted of 3' splice region accumulated mainly in the cytoplasm. This accumulation did not result from the increased stability of the RNA due to the deletion, since the wild type and mutant RNAs exhibited similar half lives after chase with actinomycin D. Therefore it is likely that the 19S spliced RNA is hindered from being transported into the cytoplasm due to some pre-splicing complexes formed at the unused 3' splice site.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.23 no.3
• /
• pp.9-16
• /
• 2019

Recently, precast PSC girder bridges have been widely applied for short and middle span bridges. The construction of the spliced girder bridges has been increasing to overcome the length limit of girder and transportation restrictions. In case of the spliced girder, the integrity of the segmental joints is very important to secure the structural soundness of bridge because the discontinuity on the segmental joints between adjacent segments could be vulnerable point. The study of segmental joint behavior with different influence factors of joint type, shear key installation, confining force is very important. In this research, finite element analysis and scaled model test with different shear key shapes and confining forces were carried out and the comparative study was performed to evaluate the segmental joint behavior of precast spliced PSC girder bridge. It was confirmed that the installation of shear key with height and depth ratio of 1/2~1/3 and applying of confining force of 1/2 of the concrete strength at the joint was effective in improving the integrity of segmental joint. In addition, the field loading test for existed precast spliced PSC girder bridge was performed and the measurement of the difference of deflection between adjacent segments at segmental joint was proposed as the assessment solution of the integrity of segmental joint.

• Proceedings of the Korea Concrete Institute Conference
• /
• 1999.04a
• /
• pp.519-524
• /
• 1999

A bulb-tee sectioned girder is proposed for long span bridge exceeding 40 meters. The proposed bulb-tee girder is developed by non-linear analysis process. This study investigates the structural behavior and efficiency of proposed bulb-tee sectioned girder using 1/2 scaled prototype beam specimen. Three specimens are tested under three point static loading system. The crack patterns, failure mode and ultimate load capacity of each specimen are reported in this paper and they are compared to each other.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.13 no.1 s.53
• /
• pp.186-194
• /
• 2009

This is a part of the extensive ongoing investigation being carried out by author to develop appropriate design procedure of the concrete member reinforced with FRP rebars instead of conventional steel rebars. This study presents the experimental results of a research programme to assess the structural characteristics of spliced rebar in reinforced concrete members with FRP reinforcement. The test variables are the diameter of FRP rebar and the embedment length. The development length (ld) was calculated according to the ACI 440 for FRP rebars in concrete. A total of 14 concrete beams reinforced with spliced FRP rebars and 4 reference beams reinforced with non-spliced FRP rebars were tested. The effects of bar size (10, 13, 16 and 19 mm) and splice length (from 0.72 to 1.58ld) on the bond strength were empirically evaluated. The test results indicate that a modification factor of 1.3 and 1.6 is relatively sufficient for the bond development length of glass FRP rebars in order to achieve an adequate tension lap splice length.

• Journal of the Korea Concrete Institute
• /
• v.16 no.1 s.79
• /
• pp.111-124
• /
• 2004

This research is a part of a research program to verify the seismic performance of circular reinforced concrete bridge columns with respect to longitudinal steel connection details under cyclic lateral load. A total of 21 column specimens were constructed and tested. Main variables in this test program were longitudinal steel connection details(continuous, lap-spliced, and mechanically connected), confinement steel ratio, and axial force ratio, etc. The test results of the columns with different longitudinal steel connection details showed different failure mode, strength degradation, and seismic performance. From the quasi-static test, it was found that the columns with all longitudinal reinforcement lap-spliced showed significantly reduced ductility. However, seismic performance of the columns with half of longitudinal reinforcement lap-spliced showed limited ductility but much more ductile behaviour than the columns with all longitudinal reinforcement lap-spliced. It was also found that the seismic performance, failure mode and strength degradation of columns with mechanical connected longitudinal reinforcement were similar to those of columns with continuous longitudinal reinforcement.

• Proceedings of the Korea Concrete Institute Conference
• /
• 2008.04a
• /
• pp.1085-1088
• /
• 2008

Splice of reinforcement is inevitable in reinforced concrete structures and, generally, lap splices are used. Lap length for tension splice is determined from development length in tension. The development length is calculated from an experimental model which was based on data of tests on anchorage and splice. Longitudinal reinforcements in flexural members are deformed and, therefore, prying action happens in spliced reinforcements unlike anchored reinforcements. The prying action induces tensile stress in cover concrete and this tensile stress plays the same role to a circumferential tensile stress caused by bond. Because splitting failure is assumed to occur when the summation of tensile stresses caused by the prying action and the bond is equal to the tensile strength of the concrete, the prying action reduces the bond strength of spliced reinforcements. A theoretical model for the prying action is developed and effects of the prying action on the bond strength are assessed. The tensile stress by the prying action is proportional to tensile strength and modulus of elasticity of reinforcements. In addition, the tensile stress is inversely proportional to spacing of reinforcements. Consequently, longer splice length is required for spliced reinforcements with small spacing in flexible members.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.11 no.5
• /
• pp.99-106
• /
• 2007

To satisfy with the increased requirements of cost reduction, labor saving, and rapid construction, the purpose of this study is to investigate the structural behavior of PSC monolithic and spliced girders. Three tests were conducted on small-scale girder specimens. This paper presents the result of experimental studies in terms of the load-deflection behavior. Different joint type and tendon amount were investigated as major variables. The monolithic girder was arranged with three tendons. The spliced girder consisted of five segments connected by three tendons. In addition, five-segmented girders connected by more than three tendons were built to examine the effect of the tendon amount. The experimental results show the difference of behavior between monolithic and spliced girders. Moreover, nonlinear finite element method analysis was utilized to verify the experimental result.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.15 no.1
• /
• pp.180-188
• /
• 2011

Recently a number of researches about mechanical splice have been studied to apply on a large diameter reinforcing steel bars of spliced sleeves. In this study the structural performance of large diameter reinforcing bars with spliced sleeves was evaluated. For the application of nuclear power plant structures, two different types of existing splices with #11, 14, 18 rebars were fabricated and static and dynamic test were performed on the basis of ASME SEC III DIV.2CC-4330.

• Earthquakes and Structures
• /
• v.16 no.3
• /
• pp.279-293
• /
• 2019

The overall seismic performance of existing pre 1960-70s reinforced concrete (RC) structures is significantly affected by the inadequate length of columns' lap-spliced reinforcement. Due to this crucial structural deficiency, the cyclic response is dominated by premature bond - slip failure, strength and stiffness degradation, poor energy dissipation capacity and low ductility. Recent earthquakes worldwide highlighted the importance of improving the load transfer mechanism between lap-spliced bars, while it was clearly demonstrated that the failure of lap splices may result in a devastating effect on structural integrity. Extensive experimental and analytical research was carried out herein, to evaluate the effectiveness and reliability of strengthening techniques applied to RC columns with lap-spliced reinforcement and also accurately predict the columns' response during an earthquake. Ten large scale cantilever column subassemblages, representative of columns found in existing pre 1970s RC structures, were constructed and strengthened by steel or RC jacketing. The enhanced specimens were imposed to earthquake-type loading and their lateral response was evaluated with respect to the hysteresis of two original and two control subassemblages. The main variables examined were the lap splice length, the steel jacket width and the amount of additional confinement offered by the jackets. Moreover, an analytical formulation proposed by Tsonos (2007a, 2019) was modified appropriately and applied to the lap splice region, to calculate shear stress developed in the concrete and predict if yielding of reinforcement is achieved. The accuracy of the analytical method was checked against experimental results from both the literature and the experimental work included herein.

• Journal of the Earthquake Engineering Society of Korea
• /
• v.10 no.1 s.47
• /
• pp.41-49
• /
• 2006

Through the 1982 Urahawa-ohi and the 1995 Kobe earthquakes, a number of bridge columns were observed to develop a flexural-shear failure due to the bond slip as a consequence of premature termination of the column longitudinal reinforcement. Because the seismic behavior of RC bridge piers is largely dependent on the performance of the plastic hinge legion of RC bridge piers, it is desirable that the seismic capacity of RC bridge pier is to evaluate as a curvature ductility. The provision for the lap splice of longitudinal steel was not specified in KHBDS(Korea Highway Bridge Design Specification) before the implementation of 1992 seismic design code, but the lap splice of not more than 50%, longitudinal reinforcement was newly allowed in the 2005 version of the KHBDS. The objective of this research is to investigate the distribution and ductility of the curvature of RC bridge column with the lap splice of longitudinal reinforcement in the plastic hinge legion. Six (6) specimens were made in 600 mm diameter with an aspect ratio of 2.5 or 3.5. These piers were cyclically subjected to the quasi-static loads with the uniform axial load of $P=0.1f_{ck}A_g$. According to the slip failure of longitudinal steels of the lap spliced specimen by cyclic loads, the curvatures of the lower and upper parts of the lap spliced region were bigger and smaller than the corresponding paris of the specimen without a lap splice, respectively. Therefore, the damage of the lap spliced test column was concentrated almost on the lower part of the lap spliced region, that appeared io be failed in flexure.