• 한국환경생물학회:학술대회논문집
• /
• 한국환경생물학회 2005년도 추계학술대회 초록집
• /
• pp.38-45
• /
• 2005

The egg bags of Korean salamander(Hynobius leechii) were collected from farmlands in Gyeongsangnam-do area. The assumed breeding time, numerical variation of embryos in each egg bag, mortality and the rates of abnormalities were investigated. The toxicity of benomyl, the metabolite carbendazim and BIC which were frequently spread in agricultural area and caused spontaneous embryonic malformation was investigated. The assumed breeding time between the end of February and the end of March has the difference about a month because of a habitat and it takes about 2 or 3 weeks from laying eggs to hatching. The length of each egg bag and the number of embryos were very varied in each area. It is due to geographical variation. Among egg bags in total study area, only 406 of egg bags(17.70% of total egg bags) developed all of embryos to normal larvae, and 78.49% of total embryos were normally developed. The patterns of spontaneous embryonic malformation were 26 species from A to Z and the abnormal patterns in individual were 8 species and above. the geographical differences about the abnormal pattern were identified and 11 habitats categorized 4 groups. The most frequent abnormality in Gyeongsangnam-do area is the dysplasia of external gill. The caudal dysplasia, abdominal blister and dysplasia of fin were also frequently observed. Individuals showing severe external defect were histologically studied and they showed retinal hypo-pigmentation, thyroid carcinoma, somatic muscular dysplasia, degeneration of cephalic neuron and various organ dysplasia. Benomyl and carbendazim were treated by 10pM$^{\sim}$10uM and BIC was treated by 1$^{\sim}$40ppm to know the effect of toxicity about toxic substance of salamander. After benomyl was treated, a survival rate was sharply dropped from 2 to 8 days. $LC_{100}$ identified in $1{\mu}M$, $LC_{50}$ identified between 100nM and $1{\mu}M$. $EC_{50}$ was assumed between 10nM and 100nM. The prevalent external malformation was abdomen swelled abnormally and histo-pathological effects were abdomen, neural tube and lens hernia. This suggests that benomyl is the toxicitic substance which inhibits the development of digestive system and nervous system. The result of treated carbendazim was similar to that of the treated benomyl. The survival rate is sharply dropped between 2 and 6 days. $LC_{100}$ was identified $1{\mu}M$ and $LC_{50}$ was identified between 10nM and 100nM. This shows that cabendazim has stronger lethal toxicity than benomyl. Ventral blister, eye dysplasia and cephalic dysplasia in the individual of external malformation mean that cabendazim affected nervous system much more than benomyl. Because the toxicity of BIC affected less in the beginning but affected more in the near hatching period, the period causing toxicity is somewhat different. $LC_{100}$ identified near 40ppm and $LC_{50}$ identified near 25ppm. The external defect shows mainly ventral blister and histo-pathological results show intestinal deformities. This result suggests the BIC inhibited strongly the development of digestive system. These abnormal developments may be caused by antimitotic action, inhibition of tubulin complex, destruction of microtubule, inhibitions of neurulation and closing of neural fold, and by the inhibition of movement of neural crest cells of benomyl. These abnormal developments may be caused by the rupture of epithelium, the loss of microtubule, the reduction of spindle size, the inhibition of spindle assembly formation, the destruction of spindle poles of carbendazim. These abnormal developments may be caused cytotoxicity by inhibition of the synthesis of a number of macromolecules and similar reaction the inhibition of benomyl.

• 재활복지공학회논문지
• /
• 제4권1호
• /
• pp.53-61
• /
• 2010

본 연구에서는 요추강화 운동기기를 이용하여 훈련 전후에 대한 유연성 및 근력 특성분석을 고찰하고, 요추 재활운동으로서 가능성을 검증하고자 한다. 요추관련 상해가 없으며 신체 건강한 성인 남녀 20명이 실험에 참여하였으며 각각 10명씩 대조군과 요추강화 운동을 제공받는 훈련군으로 나누었다. 실험에 사용된 요추강화운동기기는 하이스파인(Hi-Spine, 메디컬사이언스, 한국)이며 운동은 하루 40분씩 주 3일, 총 4주간 실시하였다. 또한 본 연구에서 피험자들은 누운 자세, 앉은 자세, 직립 자세, 전신스트레칭자세 등 총 4가지의 운동자세를 각 10분씩 제공받았다. 운동 전 후 요추의 유연성과 근력변화를 알아보기 위해 매주 1회 체간 굴곡 및 신전, 기초체력평가와 요추관절토크를 측정하였다. 실험 결과 훈련군에서 유연성과 근력이 모두 증진되었다. 요추강화운동기기의 플랫폼이 3차원 회전을 함에 따라 피험자들의 근육들은 자극받으며, 회전 방향과 각도에 따라 근긴장성과 근수축 작용을 유도시켜 유연성 및 요추 근력을 증가시키는 것으로 판단된다. 본 연구는 향후 요추관련 상해자 또는 고령자들의 요추 재활운동 프로그램에 적용될 수 있을 것이다.