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Continuous Stable production of won Willerand Factor Monoclonal Antibody in Spin Filter Bioreactor with Bleeding Technology

  • Yun, Joung-Won;Lee, Soo-Young;Park, Byung-Wook;Han-Kyu oh;Kim, Se-Ho;Byum, Tea-Ho;Park, Soung-yong
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.5 no.2
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    • pp.130-135
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    • 2000
  • The characteristics of two different modes of perfusion culture, intermittent and continuous bleedings, were investigated by culturing the hybridoma cells producing von Willebrand Factor (vWF) monoclonal antibody (McAb) in a 15 L bioreactor without clogging the filter. Both culture methods exhibited similar profiles of cell density and metabolite concentrations during the culture period at the cell concentration of around 1${\times}$107 cells/mL. When the perfusion rate was increased, the intermittrnt bleeding culture showed problems of ammonia accumulation and decrease of cell viability. The continuous bleeding culture in terms of nutrient consumption and metabolite production kinetics. But the analysis of specific oxygen consumption rate showed that the specific oxygen consumption rate of intermittent bleeding culture was similar to that of exponential growth phase. The continuous bleeding culture showed higher specific oxygen consumption rate of intermittent bleeding culture. finally we proved the possibility of long-term operation of continuous bleeding culture and produced approximately 40 g of vWF McAb in a 15L bioreactor after one-month operation.

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ELECTRICAL CHARACTERISTICS OF PENTACENE THIN FILM TRANSISTORS WITH STACKED AND SURFACE-TREATED GATE INSULATORS (러빙 처리된 표면의 적층 절연막을 가지는 Pentacene TFT의 전기적 특성)

  • Kang, Chang-Heon;Lee, Jong-Hyuk;Park, Jae-Hoon;Choi, Jong-Sun
    • Proceedings of the KIEE Conference
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    • 2002.07c
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    • pp.1546-1548
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    • 2002
  • In this paper, the electrical characteristics of pentacene thin film transistors(TFTs) with stacked and surface-treated gate insulators have been investigated. The semiconductor layer of pentacene was thermally evaporated onto the stacked gate insulators. For the gate insulating materials. PVP(PolyvinylPhenol) and polystyrene were spin-coated with two different stacking orders, PVP-polystyrene and polystyrene-PVP. Rapid solvent evaporation during the spin-coating processes of these insulating layers produces non-equilibrium phase morphologies accompanied by surface undulations on gate insulator interfaces. This non-equilibrium phase morphology affects the growth mode of the subsequent pentacene layer. Therefore, in order to smoothen the gate dielectric surfaces, gate dielectric surfaces were rubbed laterally along the direction from the drain to the source TFTs with with stacked and surface-treated gate insulators have provided improved operational characteristics.

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Analysis of Reproducing Characteristics in Perpendicular Magnetic Recording System Using Preisach Model (Preisach 모델을 이용한 수직자기기록장치의 재생특성 해석)

  • 박관수;이향범;이택동;장평우
    • Journal of the Korean Magnetics Society
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    • v.2 no.1
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    • pp.50-55
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    • 1992
  • Numerical method for analyzing the reading process in magnetic recording system is described. Hysteresis characteristics must be included in numerical analysis not only recording process but also reading process because of hysteretic behavior of recording media. Algorithm which is consisted of finite clement method for numerical analysis and Preisach model considered to be more appropriate method than Stoner-Wohlfarth model with spin curling mode for describing hysteresis characteristics is constructed. Equations to calculate waveform of reproduced voltage is proposed. Then, this is applied to perpendicular magnetic recording systems with pole type head and double layer media. Waveform of magnetic flux density and reproduced voltage induced in head coil is obtained. If the recording current increase in recording process, magnitude of reproduced voltage in reproducing process saturates. From this saturation curve, value of current which produce maximum voltage can be obtained.

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Characterization of Sol-gel Coated Pb(ZrTi)O3 Thin film for Piezoelectric Vibration MEMS Energy Harvester (압전 MEMS 진동에너지 수집소자를 위한 졸겔 공법기반의 Pb(ZrTi)O3 박막의 특성 분석 및 평가)

  • Park, Jong-C.;Park, Jae-Y.
    • Proceedings of the KIEE Conference
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    • 2009.07a
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    • pp.1240_1241
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    • 2009
  • In this paper, sol-gel-spin coated $Pb(ZrTi)O_3$ thin film with $ZrO_2$ buffer-layer and $PbTiO_3$ seed-layer was investigated for vibration MEMS energy harvester to scavenge power from ambient vibration via d33 piezoelectric mode. Piezoelectric thin film deposition techniques on insulating layer is the important key for $d_{33}$ mode of piezoelectric vibration energy harvester. $ZrO_2$ buff-layer was utilized as an insulating layer. $PbTIO_3$ seed-layer was applied as an inter-layer between PZT and $ZrO_2$ layer to improve the crystalline of PZT thin film. The fabricated PZT thin film had a remanent polarization of 5.3uC/$cm^2$ and the coercive field of 60kV/cm. The fabricated energy harvester using PZT thin film with PTO seed-layer generated 1.1uW of electrical power to $2.2M{\Omega}$ of load with $4.4V_{pvp}$ from vibration of 0.39g at 528Hz.

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Search for Dark Photon in e+e- → A'A' Using Future Collider Experiments

  • Kihong Park;Kyungho Kim;Alexei Sytov;Kihyeon Cho
    • Journal of Astronomy and Space Sciences
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    • v.40 no.4
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    • pp.259-266
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    • 2023
  • The Standard Model (SM) does not provide an information for 26% of dark matter of the universe. In the dark sector, dark matter is supposed to be linked with the hypothetical particles called dark photons that have similar role to photons in electromagnetic interaction in the SM. Besides astronomical observation, there are studies to find dark matter candidates using accelerators. In this paper, we searched for dark photons using future electron-positron colliders, including Circular Electron Positron Collider (CEPC)/CEPC, Future Circular Collider (FCC-ee)/Innovative Detector for Electron-positron Accelerator (IDEA), and International Linear Collider (ILC)/International Large Detector (ILD). Using the parameterized response of the detector simulation of Delphes, we studied the sensitivity of a double dark photon mode at each accelerator/detector. The signal mode is double dark photon decay channel, e+e- → A'A', where A' (dark photon with spin 1) decaying into a muon pair. We used MadGraph5 to generate Monte Carlo (MC) events by means of a Simplified Model. We found the dark photon mass at which the cross-sections were the highest for each accelerator to obtain the maximum number of events. In this paper we show the expected number of dark photon signal events and the detector efficiency of each accelerator. The results of this study can facilitate in the dark photon search by future electron-positron accelerators.

Analytical Solution for Attitude Command Generation of Agile Spacecraft (고기동 인공위성의 해석적 자세명령생성 기법 연구)

  • Mok, Sung-Hoon;Bang, Hyochoong;Kim, Hee-Seob
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.46 no.8
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    • pp.639-651
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    • 2018
  • An analytical solution to generate attitude command profile for agile spacecraft is proposed. In realistic environment, obtaining analytical minimum-time optimal solution is very difficult because of following constraints-: 1) actuator saturation, 2) flexible mode excitation, 3) uplink command bandwidth limit. For that reasons, this paper applies two simplifications, an eigen-axis rotation and a finite-jerk approximated profile, to derive the solution in an analytical manner. The resulting attitude profile can be used as a feedforward or reference input to on-board attitude controller, and it can enhance spacecraft agility. Equations of attitude command profile are derived in two general boundary conditions: rest-to-rest maneuver and spin-to-spin maneuver. Simulation results demonstrate that the initial and final boundary conditions, in terms of time, attitude, and angular velocities, are well satisfied with the proposed analytical solution. The derived attitude command generation algorithm may be used to minimize a number of parameters to be uploaded to spacecraft or to automate a sequence of attitude command generation on-board.

Multi-slice Multi-echo Pulsed-gradient Spin-echo (MePGSE) Sequence for Diffusion Tensor Imaging MRI: A Preliminary Result (일회 영상으로 확산텐서 자기공명영상을 얻을 수 있는 다편-다에코 펄스 경사자장 스핀에코(MePGSE) 시퀀스의 초기 결과)

  • Jahng, Geon-Ho;Pickup, Stephen
    • Progress in Medical Physics
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    • v.18 no.2
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    • pp.65-72
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    • 2007
  • An echo planar imaging (EPI)-based spin-echo sequence Is often used to obtain diffusion tensor imaging (DTI) data on most of the clinical MRI systems, However, this sequence is confounded with the susceptibility artifacts, especially on the temporal lobe in the human brain. Therefore, the objective of this study was to design a pulse sequence that relatively immunizes the susceptibility artifacts, but can map diffusion tensor components in a single-shot mode. A multi-slice multi-echo pulsed-gradient spin-echo (MePGSE) sequence with eight echoes wasdeveloped with selective refocusing pulses for all slices to map the full tensor. The first seven echoes in the train were diffusion-weighted allowing for the observation of diffusion in several different directions in a single experiment and the last echo was for crusher of the residual magnetization. All components of diffusion tensor were measured by a single shot experiment. The sequence was applied in diffusive phantoms. The preliminary experimental verification of the sequence was illustrated by measuring the apparent diffusion coefficient (ADC) for tap water and by measuring diffusion tensor components for watermelon. The ADC values in the series of the water phantom were reliable. The MePGSE sequence, therefore, may be useful in human brain studies.

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Ultrafast probes of coherent oscillations in Fe-based superconductors

  • Kim, K.W.
    • Progress in Superconductivity and Cryogenics
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    • v.19 no.1
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    • pp.1-4
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    • 2017
  • Forefront ultrafast experimental techniques have recently proven their potential as new approaches to understand materials based on non-equilibrium dynamics in the time domain. The time domain approach is useful especially in disentangling complicated coupling among charge, spin and lattice degrees of freedom. Various ultrafast experiments on Fe-based superconductors have observed strong coherent oscillations of an $A_{1g}$ phonon mode of arsenic ions, which shows strong coupling to the electronic and magnetic states. This paper reviews the recent reports of ultrafast studies on Fe-based superconductor with a focus on the coherent oscillations. Experimental results with ultrashort light sources from the terahertz-infrared pulses to the hard X-rays from a free electron laser will be presented.

Experimental Evaluation on Power Loss of Coreless Double-side Permanent Magnet Synchronous Motor/Generator Applied to Flywheel Energy Storage System

  • Kim, Jeong-Man;Choi, Jang-Young;Lee, Sung-Ho
    • Journal of Electrical Engineering and Technology
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    • v.12 no.1
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    • pp.256-261
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    • 2017
  • This paper deals with the experimental evaluation on power loss of a double-side permanent magnet synchronous motor/generator (DPMSM/G) applied to a flywheel energy storage system (FESS). Power loss is one of the most important problems in the FESS, which supplies the electrical energy from the mechanical rotation energy, because the power loss decreases the efficiency of energy storage and conversion of capability FESS. In this paper, the power losses of coreless DPMSM/G are separated by the mechanical and rotor eddy current losses in each operating mode. Moreover, the rotor eddy current loss is calculated by the 3-D finite element analysis (FEA) method. The analysis result is validated by separating the power loss as electromagnetic loss and mechanical loss by a spin up/down test.

Optical Waveguiding in the Polymerized Organic Films (유기물 박막에서의 광도파 현상)

  • 박홍준;권영세
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.19 no.5
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    • pp.5-11
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    • 1982
  • The dielectric slab waveguide is fabricated on the slide glass or oxidized silicon wafer, by spin coating polyurethane, epoxy or photoresist, and the phenomenon of dielectric waveguiding is oboerved. Using the fact that the refractive index of K.P.R. is larger than that of polyurethane, thin film prism with two layered structure is fabricated, and the refraction of light is observed.

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