• Korean Journal of Ichthyology
• /
• v.8 no.1
• /
• pp.83-89
• /
• 1996

The early life history of Korean bullhead, Pseudobagrus fulvidraco was studied to obtain some information required in aquaculture and reinforcement of natural population. The fertilized eggs were almost spherical in shape and demersal. The egg membranes were transparent with minute folds on the surface, causing them to stick to other substrates. Yolk is yellowish without oil droplets. The eggs just after fertilization were measuring $1.4{\pm}0.03mm$(1.3~1.5mm, n=10) and expanded to $1.7{\pm}0.08mm$(1.6~1.8mm, n=10) in diameter in 1.5 hr. The blastodisc was formed in 30 min and cleavage started in 1 hr after fertilization, and the intervals of each stage of cleavage was about 30 min at $25.0^{\circ}C$. The yolk from 32-cell stage to gastrula stage partly depressed and the depressed part moved clockwise. Hatching occurred in 53 hr after fertilization and hatched embryos had 18~19+20~21(38~40) myomeres measuring 4.2~4.3mm in total length. At the age 7 d after hatching, 4 pairs of barbels were already formed ; 1 pair on the posterior part of the nostril, 1 pair on the upper jaw, and 2 pairs on the lower jaw. And the posterior margin of caudal fin changed into two folds. The lateral band and the form of all fins were completed in 3 weeks.

• Applied Microscopy
• /
• v.33 no.3
• /
• pp.243-250
• /
• 2003

Ultrastructural changes of the male germ cells and structure of spermatozoa in Paralichthys olivaceus were examined by means of the light and transmission electron microscopes. The spermatogonium has a large nucleus with a single nucleus with a single nucleolus in the interphase. Primary spermatocytes are identified by the formation of the synaptonemal complex in the karyoplasm. The secondary spermatocytes are more concentrated and contains numerous cell organelle in the cytoplasm. The nucleus of spermatid in spermiogenesis is more condensed in the karyoplasm, and show spherical structure in shape. Mitochondria of the spermatids are observed in the lower portion of the nucleus. The spermatozoon consists of the head, mid piece and tail. The acrosome is not observed in the head. Axial filaments of the flagellum consists of nine pairs of the peripheral microtubules and one pair of the central microtubules.

• Parasites, Hosts and Diseases
• /
• v.31 no.3
• /
• pp.183-192
• /
• 1993

A transmission electron microscopic study was performed to observe the ultrastructures of the male germinal cells and spermatozoa of Fibricola seoulensis. Spermatogonia were found in the periphery of the testis and characterized by large nuclei and comparatively little cytoplasms. Spermatocytes contained an oval to spherical nucleus. Their nuclear volume was little larger in comparative to that of cytoplasm, and the chromatin was comparatively little. The early spermatids were characterized by a great amount of cytoplasm, and numerous mitochondria encircled the nucleus. In a more advanced spermatids the electron-dense strands of chromatin appeared in the nucleus, and a pair of rootlet of the axoneme and a microtubule-organizing center (MTOC) were observed near the nucleus. The sectioned spermatozoa were found in the testis and the seminal vesicle. Their cross sectional views were divided into 6 types when they were distinguished on the basis of the morphology and components. The spermatozoa of F. seoulensis showed two flagella of 9+1 type axoneme.

• The Korean Journal of Malacology
• /
• v.26 no.4
• /
• pp.311-316
• /
• 2010

Spermatid differentiations during spermiogenesis and mature sperm ultrastructure in male Crassostrea nipponica were investigated by transmission electron microscope observations. The morphology of the spermatozoon of this species has a primitive type and is similar to those of other bivalves. Mature spermatozoa consist of broad, cap-shaped acrosomal vesicle and an axial rod in subacrosomal materials on an oval nucleus showing deeply invaginated anteriorly, two triplet substructure centrioles surrounded by four spherical mitochondria, and satelite fibres, which appear near the distal centriole. The acrosomal vesicle of spermatozoa of C. nipponica resemble to those of other investigated ostreids. Especially, two transverse bands (stripes) appear at the anterior region of the acrosomal vesicle, unlikely 2-3 transverse bands (stripes) in C. gigas. It is assumed that differences in this acrosomal substructure are associated with the inability of fertilization between the genus Crassostrea and other genus species in Ostreidae. Therefore, we can use sperm morphology in the resolution of taxonomic relationships within the Ostreidea. The sperm is approximately $48-50{\mu}m$ in length including an oval sperm nucleus (about $1.0{\mu}m$ in length and $1.41{\mu}m$ in width), an acrosome (about $0.48{\mu}m$ in length and 0.30 in width) and tail flagellum ($46-48{\mu}m$). The axoneme of the sperm tail flagellum consists of nine pairs of microtubules at the periphery and a pair at the center. The axoneme of the sperm tail shows a 9 + 2 structure. These morphological charateristics of acrosomal vesicle belong to the family Ostreidae in the subclass Pteriomorphia.

• The Korean Journal of Malacology
• /
• v.21 no.2 s.34
• /
• pp.95-105
• /
• 2005

Gonadosomatic index, reproductive cycle, spermatogenesis and first sexual maturity of Chlamys farreri were investigated by cytological and histological observations, from January 1998 to December 1999. The gonadosomatic index (GSI) rapidly increased in April and reached a maximum in May when seawater temperature rapidly increase. Then the GSI gradually decreased from June to August when spawning occur. Accordingly, monthly changes in the GSI in males coincide with the reproductive cycle. The spermatozoon of Chlamys farreri is the primitive type found in external fertilization species. The head of the spermatozoon is approximately $2.75{\mu}m$ in length including the acrosome measuring about $0.50{\mu}m$ in length, and its tail was approximately $20{\mu}m$, the axoneme of the tail flagellum consists of nine pairs of microtubules at the periphery and a pair at the center. Five spherical mitochondria around the centriole (the satellite body) appear in the middle piece of the sperm. The spawning period was from June to August and the main spawning occurs from July to August when seawater temperatures are greater than $20^{\circ}C$ The reproductive cycle of this species can be categorized into five successive stages; early active stage (January to March), late active stage (March to April), ripe stage (April to August), partially spawned stage (June to August), and spent/inactive stage (August to January). Over 50% of male scallops attained first sexual maturity between 50.0 and 60.0 mm in shell height, and 100% of those over 60.0 mm in shell height achieved maturity. Accordingly, we assume that male individuals begin reproduction at three years of age.

• The Journal of the Acoustical Society of Korea
• /
• v.15 no.5
• /
• pp.65-72
• /
• 1996

A dynamic self-consistent method previously proposed and validated for the composites containing spherical inclusions is applied to the simplest two dimensional problems : SH wave propagation in unidirectional fiber reinforced composites. The self-consistent conditions for SH wave are derived without limitation on frequency and the wave speed and coherent attenuation are calculated for two composites. THe results of the present theory are compared with those of the multiple scattering theories and another self-consistent theory. At low volume fractions, the present theoretical results coincide with those of the multiple scattering theory using exact pair-correlation function, whereas the results based on another self-consistent theory deviate markedly from the others. As the volume fraction increases, the three theories give different results although they have qualitatively similar trends. The present theoretical results for composites considered in this paper exhibit less dispersion and physically realizable attenuation. An important observation is that the multiple scattering theory predicts vanishingly small attenuation at low frequency with volume fraction is high.

• Transactions on Electrical and Electronic Materials
• /
• v.2 no.2
• /
• pp.37-41
• /
• 2001

Photo catalytic characteristics of nano-sized TiO$_2$ powder with rutile phase produced using homogeneous precipitation process at low temperatures (HPPLT) were compared with those of commercial P-25 powder by Degussa Co. The TiO$_2$ powder by HPPLT showed very higher photoactivity in the removal rate, showing lower pH values in the solution, than the P-25 powder when eliminating metal ions such as Pb and Cu from aqueous metal-EDTA solutions. This can be inferred the more rapid photo-oxidation or -reduction of metal ions from the aqueous solution, together with relatively higher efficiencies in the use of electron-hole pair formed on the surface of TiO$_2$ particle, under UV light irradiation. Also, in the view of the TiO$_2$ particle morphology, compared to the well-dispersed spherical P-25 particle, the agglomerated TiO$_2$ particle by HPPL T consists of acicular typed primary particle with the thickness ranged of 3∼7 nm, which would be more effective to the photocatalytic reactions without electron-hole recombination on the surface of the TiO$_2$ particle under the UV light irradiation. It is, therefore, thought that the higher photo activity of the rutile TiO$_2$ powder by HPPLT in the aqueous solutions resulted from having its higher specific surface area as well as acicular shape primary particle with very thin thickness.

• Applied Microscopy
• /
• v.24 no.3
• /
• pp.1-9
• /
• 1994

Pardosa astrigera possessed eight eyes arranged in three rows on the frontal carapace. A pair of small anterior lateral eyes (ALE) flanked each side by an anterior median eyes (AME) lay along the anterior margin that was situated on the anterior row of clypeus. The anterior lateral eye was composed of cornea, vitreous body, and retina. Cornea was made up mainly of exocuticle lining the cuticle. Lens in anterior lateral eye was biconvex type which bulged into the cavity of the eyecup. Outer and inner central region of lens were approximately spherical with radius of curvature $5.6{\mu}m$ and $12.5{\mu}m$, respectly. Vitreous body formed a layer between the cuticular lens and retina. They formed biconcave shape. Retina of the anterior lateral eyes was composed of three types of cells: visual cells, glia cells, and pigment cells. The visual cells were unipolar neuron, as were the receptor of the posterior lateral eye. But cell body was unique to the anterior lateral eyes. They were giant cell, relatively a few in number, and under the layer of vitreous bodies. Each visual cell healed rhabdomeres for a short stretch beneath the cell body. Rhabdomes were irregulary pattern in retina and electron dense pigment granules scattered between the rhabdomes. Glia cell situated at the cell body of visual cell and glia cell process reached to rhabdomere portion. Below the rhabdome, tapetum were about $30{\mu}m$ distance from lens, which composed of 4-5 layers. It was about $25{\mu}m$ length that intermediate segment of distal portion of visual cell. Electron dense pigment granules between the intermediate segment were observed.

• Development and Reproduction
• /
• v.2 no.1
• /
• pp.101-109
• /
• 1998

Spermatogenesis and reproductive cycle of the razor clam, solen grandis, were investigated monthly by histological and cytological observations. Samples were collected from natural intertidal population at Oshik-do, Kunsan, Korea, for one year, beginning from January to December, 1993. solen grandis is dioecious. Morphological structures of the spermatozoon of this species ar esimilar to those of other bivalve spermatozoa having a primitive type; i.e., a small head, a cap-shaped acrosome and a short mid-piece with four mitochondria surrounding axial filament. The head of spermatozoon is approximately 2 \mu m in length and sperm tail is about 20 \mu m long. The axoneme of tail flagellum consists of nine pairs of peripheral microtubules at the periphery and a pair of central microtubules at the center. Four spherical mitochondria form the paranucleus. Spawning occures once a year between early June and July, and the main spawning was observed in July when seawater temperature reaches above 20 \circ C. The reproductive cycle of male razor clam can be divieded into fivesuccessive stages; early active (December to january), late active (January to march), mature (March to early August), partially spawned (June to July), and spent/inactive stage (August to December).

• Applied Microscopy
• /
• v.40 no.2
• /
• pp.109-116
• /
• 2010

Morphology and microstructure of the suction trap in aquatic Utricularia japonica were examined using scanning electron microscopy. Branched stems bear numerous suction traps without root formation. The traps are derived axillary from the node, and their antennae and appendages extend in a peculiar fashion. The trap walls are thin, two-celled, parenchyma tissue and simple, small glands are scattered in both internal and external surface of the trap. The entrance of the trap is surrounded by one pair of dorsal antennae and ventral appendages, where the former guides the prey to the entrance. Trap door is situated below the entrance and numerous sessile and stalked capitate trichomes cover the entrance and even on the door surface. The capitate trichomes are secretory, but four trigger hairs formed on the central areas of the door are not. They are believed to function in activating and tripping the trap door. A specialized region of the threshold come in contact with the lower portion of the door upon closing. The secretory capitate trichomes near this region are responsible for producing and secreting a mucilage-like substance which composes the velum. Two-armed bifid glands are located in the interior side of the threshold, while four-armed quadrifid glands are considerably numerous occurring over the entire inner trap wall. Bifid and quadrifid glands develop semi-spherical basal cells that connect them to the inner wall surface. Antennae, trigger hairs, capitate trichomes, bifid and quadrifid glands are more important structures in the carnivory of U. japonica.