• Applied Microscopy
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• 제26권2호
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• pp.221-233
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• 1996

In order to study process of spermiogenesis of the Korean manchurian field mouse, Apodemus spesiosus peninsulae, the testis obtained from sexually matured male reproductive organs, were examined with electron microscopy, and the following results were obtained based on the characters of cell differentiation. 1. According to the features of cell structure, spermiogenesis of the Apodemus spesiosus peninsulae was five phases: Golgi, cap, acrosome, maturation and spermiation phase. They were further subdivided into two steps of early and late phases respectively. Hence, the spermiogenesis consists of ten steps. 2. In the changes of the chromatin, the chromatin granules began to be condenced in the cap phase and regularizated at maturation phases, and a perfect nucleus of sperm was formed at the spermiation phases. 3. The formative period of sperm tail began to be develop in the early Golgi phase and completed at the spermiation phases 4. The outer dence fibers of middle piece were arranged in a horseshoe fashion. Nos. 1, 5, 6 and 9 of the outer dense fibers were larger than the others. The structure of axoneme in the middle piece was 9+2, and the axonemal complex consists of A and B microtubules, dynein arms and radial links.

• Applied Microscopy
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• 제25권1호
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• pp.96-110
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• 1995

In order to clarify the process of spermiogenesis of the large-footed bat, Myotis macrodactylus, the testis and the epididymis obtained from mature male bats were examined by electron microscope. Based on the variety and diagnostic characters of organells, the spermiogenesis of the large-footed bat. Myotis macrodactylus could be divided into a total of nine phases. The results obtained from the present study are as follows. 1. The spermiogenesis of large-footed bat, Myotis macrodactylus was divided according to the level of fine structural differentiation into five phases, Golgi, cap, acrosome, maturation and spermiation phases, respectively; Golgi, cap, acrosome and spermiation phases were further subdivided into steps of early and late phase respectively and maturation phase has only one step. Hence, the spermiogenesis of the large-footed bat has been divided into a total of nine phases. 2. In the change of chromatin with nucleus, the chromatin granules are condensed in the whole part of nucleus at the late Golgi phase and completed at the maturation phase. 3. The sperm tail in the epididymis consists of nine outer doublets and two central singlet microtubles. Nos. 1, 5, 6, 9 of the outer dense fibers were larger than the others (2, 3, 4, 7, 8).

• 한국가축번식학회지
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• 제9권1호
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• pp.46-55
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• 1985

In order to investigate the spermiogenesis of the Korean goat, the tissue obtained from the testis and the epididymis of eight matured bucks were conventionary prepared as thin sections and these sections were observed under a transmission electron microscope. Based on the development of the varous permanent organelles and certain transient structures, the spermiogenesis of the Korean goat could be divided into four main phases: Golgi, cap, acrosome and maturation; and these phases had been further subdivided into a total of nine stages. There was abundance of the contents of acrosomal vesicle contained ring-shaped material surrounding the acrosome in the early cap stage. The mitochondria made the mitochondrial sheath started from the annulus towarding the neck and formed helical arrangement in the mid maturation stage, The postacrosomal denselamina was a, pp.ared to be wave-shape in the late maturation stage. The spermiogenesis of the Korean goat was found to be basically similar to that of the Korean cattle.

• Applied Microscopy
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• 제22권2호
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• pp.97-117
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• 1992

In order to study process of spermiogenesis of the Korean greater horseshoe bat, Rhinolophus ferrumequinum korai, the cycle of seminiferous epithelium was examined by the light and electron microscope and the following results were obtained based on the epithelial cell differentiation. 1. Spermiogenesis occurred from early July to mid-Octber, and spermatogenic activity was vigorous from mid-August to late September. Spermatocytes including spermatogonia were found to be degenerated in only July. It is deduced that the degeneration serves as the mechanism to regulate effective use of energy to prepare for mating and hibernating periods, and regulation of breeding cycle. 2. Spermiogenesis of the Korean greater horseshoe bat was divided according to differentiation of the cell structure, into Golgi, cap, acrosome, maturation and spermiation phases; Golgi, cap and spermiation phases were further divided into two steps of early and late phase respectively, and acrosome phase into three steps of early, mid and late phases, and maturation phase has only one step. Hence, the spermiogenesis consists of ten phases. The first research was done in this article on the changes of chromatin with nucleus, the time of appearance and disappearance of chromatin granules, in case of Korean greater horseshoe bat (Rhinolophus ferrumequinum korai). Chromatin granule began to be condensed in late Golgi and the condensation proceeded to form an irregular mass of a electron-dense chromatin in a form of circular cylinder in the center of nucleus at the phase of maturation. Finally, the chromatin condensation proceeded and perfect nucleus of sperm with homogeneous density was formed when the sperm was separated from Sertoli cell. Therefore, appearance and disappearance of chromatin granules occurred in the period of time between late Golgi and maturation phase, The tail of sperm began to develop in early cap phase, Numerous lipid droplets were obseved in the cytoplasm of spermatids during the maturation phase, which seemed to be used as energy source necessary to make mature sperm during spermiogenesis.

• 한국발생생물학회지:발생과생식
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• 제1권1호
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• pp.9-24
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• 1997

To investigate the process of spermiogenesis of the Korean eastern Daubenton's bat, Myotis daubentonii ussuriensis, the testis obtained from mature male bats was studied by transmission electron microscope and were based on the variety and diagnostic characters of cell organells. The results obtained from the present study are as follows. According to the differentiation of the cell organells, the spermiogenesis of the Korean eastern Daubenton's bat, M. d. ussuriensis, was divided into Golg, cap, acrosome, maturation and spermiation phases. Besides, these Golgi, cap, acrosome, and maturation phase were subdivided into the steps of early and late phases repectively and matruation phase was subdivided into step of early, mid and late phases. Therfore, the spermiogenesisof M. d. ussuriensis has been divided into a total of 11 phases. The chromatin granules began to condense at the early cap phase, regularized at the acrosome phase, and a perfect nucleus of sperm was formed at the maturation phase. The chromatoid body was occurred in the upper cytoplasm of nucleus at the early Golgi phase, and it was accurred the posterior cytoplasm of the nucleus at the early maturatio phase. The formation of sperm tail began to be develop in the early golgi phase, and completed at the spermiation phase. The fiber structure of middle piece was consisted of nine outer doublets and two central singlet microtubules and Nos. 1, 5, 6 and 9 in the outer dense were larger than the others(2, 3, 4, 7, 8).

• 한국수산과학회지
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• 제40권3호
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• pp.147-152
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• 2007

Spermiogenesis and mature spermatozoa of the Korean false minnow Pseudogobio esocinus (Cyprinidae) are described by means of scanning and transmission electron microscopy. Spermiogenesis is characterized by lateral development of the flagellum, absence of nuclear rotation, and eccentric nuclear fossa formation. The spermatozoa have a spherical head containing a nucleus with highly condensed chromatin and no acrosome. The nuclear fossa contains the proximal centriole and anterior part of the distal centriole. The midpiece is type A II and contains semi-fused mitochondria around the axoneme. However, the symmetrical distribution of 4 or more the mitochondria does not follow a general pattern of the cyprinid spermatozoa. Cytoplasmic vesicles in the midpiece are common in Cypriniformes and some of Siluriformes and Characiformes spermatozoa.

• Applied Microscopy
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• 제31권2호
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• pp.143-156
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• 2001

본 연구는 황소개구리의 정소내 생식세포분화 단계와 정자변태과정중의 정자의 형태적 특징을 알아보기 위하여 조사한 결과 다음과 같은 결론을 얻었다. 황소개구리의 정자형성과정 중 생식세포는 제1정원세포, 제2정원세포, 제1정모세포, 제2정모세포 및 정자세포로 구성되어져 있으며, 이들 생식세포의 분화단계는 세포의 형태적 특정을 기초로 하여 총 8단계로 구분되어졌다. 제1정원세포를 제외한 정모세포발생 단계에서부터 이탈 전까지의 정자세포는 정낭내에 존재하고 있었다. 정자변태과정은 3단계로 구분되어졌다. 성숙기의 정자의 첨체는 낭상이고, 두부의 모양은 양끝이 가는 원통형이었으며, 꼬리는 단지 축사로만 구성되어져 있었다.

• 한국어류학회지
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• 제21권2호
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• pp.81-86
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• 2009

한국산 동자개 Pseudobagrus fulvidraco의 정세포변형과 성숙한 정자의 미세구조를 주사 및 투과전자현미경으로 관찰하였다. 정세포변형은 측면에서 발생되는 편모, 핵의 회전, 염색물질로 채워진 핵의 함입과 핵와의 형성이 특징적이었다. 성숙한 정자의 구조는 첨체가 없는 둥근두부와 짧은중편 그리고 긴 편모로 구성되어 있었다. 짧은 중편에는 다수의 미토콘드리아가 있으며 cytoplasmic canal에 의하여 편모와 분리되어 있었다. 편모는 전형적인 9+2 구조이며 axonemal fins를 가지고 있었다. Axonemal fins는 동자개과에서 공통적으로 나타나는 형질로 여겨지며 그 외 다른 특징을 경골어류에서 비교하였다.

• 한국수산과학회지
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• 제32권6호
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• pp.730-736
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• 1999

물가자미의 정자변태과정과 정자의 미세구조를 주사전자현미경과 투과전자현미경으로 조사한 결과는 다음과 같다. 정자변태과정동안 염색질은 과립형태에서 구상으로 점차 응축하여 성숙 정자의 두부에서는 치밀하고 균질하게 된다. 물가자미 정자변태과정 동안 나타나는 특징은 세포질에서 골지체의 소실, 미세섬유의 출현, 미토콘드리아 수의 감소, 용해소체의 출현이다. 이 과정에서 미세섬유는 정자의 형태형성에 관여하며, 골지체의 소실과 미토콘드리아수의 감소는 용해소체의 작용에 의한 것이다. 정자는 두부와 미부로 구성되며, 무첨체형이다. 두부 후방에서는 cytoplasmic collar가 관찰되었으며, cytoplasmic collar는 7개의 미토콘드리아를 가진다. 미부에서는 잘 발달된 axonemal lateral fin을 관찰할 수 있다. 미부 편모축사의 횡단면은 "9+2"의 미세소관 구조를 나타내며, 세포질내에 많은 수의 공포를 가진다.

• Applied Microscopy
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• 제31권2호
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• pp.129-141
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• 2001

식충목 땃쥐과 쇠뒤쥐 (Sarex minutus gracillimus)의 정자변태과정을 알아보기 위하여 세정관 상피의 세포분화에 따른 형태적 특징을 조사하여 다음과 같은 결과를 얻었다. 쇠뒤쥐의 정자변태과정을 골지기, 두모기를 각각 전 중 후기로, 첨체기를 각각 전 후기로, 성숙기와 이탈기를 각각 하나의 단계로 총 10단계로 나눌 수 있었다. 핵질의 응축은 첨체기에 시작하여 이탈기에 완성되었으며, 만세트의 출현은 첨체후기부터 성숙기까지 나타났다. 정자편모의 형성은 골지후기에 생성하여 이탈기에 완성되었다. 다포체는 골지기부터 성숙기까지 나타났으며, 골지기는 주로 pale multivesicular body가, 두모기와 첨체기는 주로 pale 또는 moderate pale multivesicular bodies가 그리고 성숙기에서는 dense multivesicular body가 관찰되었다.