• Korean Journal of Veterinary Research
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• v.29 no.4
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• pp.417-424
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• 1989

This study was conducted in order to observe the changes in cellular association of seminiferous tubules from 4 to 22 weeks of age and to obtain the cycle and relative duration of seminiferous epithelia from 24 weeks of age in male ducks. Fifety-five male ducks were used in the experiment and divided into 11 groups, consisting of 5 male ducks each, with 2 weeks intervals from 4 to 24 weeks of age. The results were summarized as follows: 1. The body and testes weight showed most rapid increase during 4 to 6 weeks and 18 to 22 weeks of age, respectively. The seminiferous tubules were obruptly enlarged in diameter of tubules during 18 to 22 weeks of age. 2. Gonocytes were seen from 4 to 6 weeks of age, however they were not observed as from 8 weeks of age. Both type Ap spermatogonia and type Ad spermatogonia occured from 8 to 12 weeks of age, while spermatocytes and spermatids were beginning to appear at 16 weeks and 18 weeks of age, respectively. Spermatozoa were first observed at 20 weeks of age. Full spermatogenic activity was completed at the age of 20 weeks. 3. Average paired weight of the testes in male ducks was 78g at 24 weeks of age and its ratio to the body weight was approximately 2.5 percent. 4. Average diameter of seminiferous epithelium at 22 weeks of age was $232{\mu}m$, and average numbers of Sertoli cell, spermatogonia, spermatocyte, spermatids and spermatozoa in the cross section of seminiferous epithelium were 15.30, 59.08, 41.78, 71.11 and 165.30, respectively. Spermatogonia and spermatids were classified into 2 and 4 types, respectively. 5. The cycle of the seminiferous epithelium could be divided into 5 stages at 24 weeks of age. The relative frequencies of stages from I to V were 13.5%, 25.0%, 22.3%, 20.6% and 18.7% respectively. Thus, establishment of spermatogenesis in male ducks were beginning to appear at 20 weeks of age.

• Korean Journal of Veterinary Research
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• v.35 no.3
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• pp.563-573
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• 1995

To know the effect of Ivermectin(IVM) toxicity in testis, histopathologic changes as well as clinical signs were observed in experimental animals including dogs by the subcutaneous injection with 3-50mg/kg of IVM. Clinically, it was observed to have depression and ataxia in all groups whereas tremor and coma in mice, rats and guinea pigs, coma in hamsters and rabbits, and tremor and salivation in dogs were shown. The clinical signs were different by the dosage of IVM, species and individuals in all animals. Susceptibility to IVM was most sensitive in dogs, especially in a Tosa dog and this was susceptible in mice, hamsters and rabbits, guinea pigs and rats in order. Microscopical observation revealed that the seminiferous tubules of testis had decreased thickness of germinal epithelium due to the necrosis and desquamation of the spermatids and spermatocytes. The progressive pattern by the times of administration showed vacuolar formation between the layer of spermatids and spermatogonia due to the marked necrosis of spermatocytes and the presence of multinucleated giant cells derived from spermatid throughout the seminiferous tubules of testis. Only a layer of spermatogonia, a few spermatogonia, and Sertoli cells wore observed with atrophied wavelike basement membrane in the seminiferous tubules of testis. Necrotic germinal cells, sloughed immature spermatids and spermatocytes were present in the lumen of epididymis and ductus deferens. Microscopical observation showed different susceptibility to IVM with clinical observation in which it was also most sensitive in dogs, especially in a Tosa dog and this was susceptible in rabbits and guinea pigs, hamsters, rats and mice in order. It was considered that IVM affects mainly spermatocyte or spermatid stage in the spermatogenesis and disturbs their developing beyond these stage.

• The Korean Journal of Zoology
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• v.24 no.2
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• pp.65-75
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• 1981

The formation of the acrosome during spermatogenesis in Gerris paludum was studied. The Golgi bodies are dispersed randomly in the cytoplasm at the early stage of the spermatocyte and get together to form several group of many bodies, and then they are equally divided into the spermatids by the meiotic divisions. The acroblast first appears in the form of a vesicle and soon an acrosomal granule is differentiated within it. The acroblast is separated from the acrosomal granule at the posterior of the nucleus and is finally sloughed off along the tail filament. The acrosome, after moving to the side of the nucleus opposite the mitochondrial derivatives, differentiates into two zones. The two basal bodies and the differentiated tip originate from the sheath. The basal bodies appear at the proximal part of the sheath simply in contact with the core on one side. During elongation and and narrowing of the acrosomes of the spermatids, they surround the one side at the base of the acrosome and finally all the other are immediately adjacent to the nucleus. The differentiated tip continues to the sheath at the anterior of the cores and is elongated prior to the two basal bodies. They appear to be contiguous twin-tubes, not a single granule in the later stage of the spermatids, and a group of the basal bodies in the sperm bundle.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2003.10a
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• pp.133-133
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• 2003

• Development and Reproduction
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• v.10 no.1
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• pp.9-17
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• 2006

The cycle of the seminiferous epithelium and morphological features of spermatids in Crocidura dsinezumi were studied by light microscopy. The cycle of the seminiferous epithelium was divided into 12 stages. The dark type of spermatogonium(Ad) is appeared in all stages, and intermediate(In) in stage IV and B spermatogonium in stage V and VI were observed. The development of the acrosomal system, and changes in nuclear morphology of spermatids were divided into 14 steps. The Golgi, cap, acrosomal, maturation and spermiation phases were observed during steps $1{\sim}2$, steps $3{\sim}6$, steps $7{\sim}10$, steps $11{\sim}13$, and step 14, respectively. Our results provide the foundation for future studies of the spermiogenesis of Crocidura dsinezumis.

• Molecules and Cells
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• v.26 no.6
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• pp.621-624
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• 2008

We previously showed that Asb-4 and Asb-17 is uniquely expressed in developing male germ cells. A recent report showed that Asb-9 is specifically expressed in the kidney and testes; however, detailed expression patterns in developing germ cells have not been shown. Northern blot analysis in various tissues demonstrated that mAsb-9 was strongly expressed in the testes. Expression analysis by RT-PCR and Northern blot in developing mouse testes indicates that mAsb-9 is expressed from the fourth week after birth to adulthood, with the highest expression in round spermatids. Expression sites were further localized by in situ hybridization in the testes. Pachytene spermatocytes and spermatids expressed mAsb-9 but spermatogonia and generated spermatozoa did not. This study reveals that mAsb-9 could be a specific marker of active spermatogenesis and would be useful for studies of male germ cell development.

• Applied Microscopy
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• v.31 no.2
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• pp.143-156
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• 2001

The aim of this study was to clarify the differentiation of seminiferous epithelial cells and spermiogenesis in the testis of Rana catesbeiana. Spermatogenesis of R. catesbeiana consists of primary spermatogonia, secondary spermatogonia, primary spermatocytes, secondary spermatocytes and spermatids. They were subdivided into eight stages on the basis of the morphological features of the germ cell differentiation. From the spermatocytes except primary spermatogonia to before the spermiation of spermatids were surrounded by spermatocyst. Spermiogenesis of R. catesbeiana can also be divided into three stages on the basis of morphological features of the nucleus and the cytoplasm organelles. Spermatozoon contained a saccular acrosome, a cylindrical and tapered slighty at both ends head, and a tail with only the axoneme.

• Korean Journal of Veterinary Research
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• v.26 no.2
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• pp.201-210
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• 1986

The cycle of the seminiferous epithelia in the testis of mature Korean native cattle was divided into twelve stages, using criteria the morphological changes in the acrosomic system and the nuclei of developing spematids and germ cells. The results were summarized as follows; 1. The minimum number of tripe A spermatogonia were the average of 1.8 in both at stages I and VI, while maximum numbers were the average of 4.2 at stage XII. Some type A spermatogonia divided at stage XII to produce the type intermediate(IN) spermatogonia at following stage I. The intermediate type spermatogonia divided at stage IV to produce the type B spermatogonia at stage V. 2. The type B spermatogonia divided at stage VII to produce the preleptotene primary spermatocytes at stage XII. The pachytene primary spermatocytes divided at stage XI to produce the secondary spermatocytes at stage VII. The secondary spermatocytes observed at stag XII divided to give rise to the round spermatids at following stage I. Each numbers of the first spermatocytes and of spermatids were almost constant, respectively, through the cycle of the seminiferous epitherium. 3. The relative frequencies of each stage among stages I to XII of the cycle of the seminiferous epithelia were 6.1, 3.7, 5.2, 7.8, 2.2. 3.3, 13.8, 18.4, 11.8, 7.2, 18.1% and 2.4%, respectively.

• Development and Reproduction
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• v.26 no.2
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• pp.59-69
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• 2022

Many efforts have been made to study the expression of aquaporins (AQP) in the mammalian reproductive system, but there are not enough data available regarding their localized expression to fully understand their specific roles in male reproduction. The present study investigated the expression and localization patterns of different AQP subtypes in the adult mouse testes and testicular spermatozoa using an immunofluorescence assay. All the studied AQPs were expressed in the testes and revealed subtype-specific patterns in the intensity and localization depending on the cell types of the testes. AQP7 was the most abundant and intensive AQP subtype in the seminiferous tubules, expressing in Leydig cells and Sertoli cells as well as all stages of germ cells, especially the spermatids and testicular spermatozoa. The expression pattern of AQP3 was similar to that of AQP7, but with higher expression in the basal and lower adluminal compartments rather than the upper adluminalcompartment. AQP8 expression was limited to the spermatogonia and Leydig cells whereas AQP9 expression was exclusive to tails of the testicular spermatozoa and elongated spermatids. Taken together, the abundance and distribution of the AQPs across the different cell types in the testes indicating to their relavance in spermatogenesis, as well as in sperm maturation, transition, and function.

• Development and Reproduction
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• v.26 no.3
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• pp.107-115
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• 2022

Kisspeptins, products of KISS1 gene, are ligands of the G-protein coupled receptor (GPR54), and the kisspeptin-GPR54 signaling has an important role as an upstream regulator of gonadotropin releasing hormone (GnRH) neurons. Interestingly, extrahypothalamic expressions of kisspeptin/GPR-54 in gonads have been found in primates and experimental rodents such as rats and mice. Hamsters, another potent experimental rodent, also have a kisspeptin-GPR54 system in their ovaries. The presence of testicular kisspeptin-GPR54 system, however, remains to be solved. The present study was undertaken to determine whether the kisspeptin is expressed in hamster testis. To do this, reverse transcription-polymerase chain reactions (RT-PCRs) and immunohistochemistry (IHC) were employed. After the nest PCR, two cDNA products (320 and 280 bp, respectively) were detected by 3% agarose gel electrophoresis, and sequencing analysis revealed that the 320 bp product was correctly amplified from hamster kisspeptin cDNA. Modest immunoreactive (IR) kisspeptins were detected in Leydig-interstitial cells, and the weak signals were detected in germ cells, mostly in round spermatids and residual bodies of elongated spermatids. In the present study, we found the kisspeptin expression in the testis of Syrian hamster. Further studies on the local role(s) of testicular kisspeptin are expected for a better understanding the physiology of hamster testis, including photoperiodic gonadal regression specifically occurred in hamster gonads.