• 대한의생명과학회지
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• 제11권1호
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• pp.85-88
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• 2005

Hemoglobin is oxygen carrier protein within erythrocyte in blood. Apoprotein of this, globin, is synthesized in the cytosol but it's cofactor, heme, is synthesized in the mitochondria. It has not been known very well how globin receives the heme from mitochondria and folds to hemoglobin. In this folding process, the initial structure of globin seems to be very important. A small volume of globin at acid pH was added rapidly into the bulk of an egg phosphatidylcholine $60\%$ liposome, containing hemins, at neutral pH according to the Rapid Dilution method. It was observed that an acid-induced unfolding structure of globin is initially needed to receive hemins from the lipid bilayer of liposomes. Also, this conclusion was confirmed with the absorption spectrum of the refolded globin separated by centrifugation.

• Asian Pacific Journal of Cancer Prevention
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• 제13권4호
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• pp.1705-1708
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• 2012

The inception of targeted agents has revolutionized the cancer therapy paradigm, both for physicians and patients. A large number of molecular targeted agents for cancer therapy are currently available for clinical use today. Many more are in making, but there are issues that remain to be resolved for the scientific as well as social community before the recommendation of their widespread use in may clinical scenarios can be done, one such issue being cost and cost effectiveness, others being resistance and lack of sustained efficacy. With the current knowledge about available targeted agents, the growing knowledge of intricate molecular pathways and unfolding of wider spectrum of molecular targets that can really matter in the disease control, calls for only the just use of the agents available now, drug companies need to make a serious attempt to reduce the cost of the agents. Research should focus on agents that show sustained responses in preclinical data. More needs to be done in laboratories and by the pharmaceutical industries, before we can truly claim to have entered a new era of targeted therapy in cancer care.

• Nuclear Engineering and Technology
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• 제49권6호
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• pp.1354-1357
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• 2017

Characterization of special nuclear material may be performed using energy spectroscopy of either the neutron or gamma-ray emissions from the sample. Gamma-ray spectroscopy can be performed relatively easily using high-resolution semiconductors such as high-purity germanium. Neutron spectroscopy, by contrast, is a complex inverse problem. Here, results are presented for $^{252}Cf$ and PuBe energy spectra unfolded using a single EJ309 organic scintillator; excellent agreement is observed with the reference spectra. Neutron energy spectroscopy is also possible using a two-plane detector array, whereby time-of-flight kinematics can be used. With this system, energy spectra can also be obtained as a function of position. Spatial-dependent energy spectra are presented for neutron and gamma-ray sources that are in excellent agreement with expectations.

• Journal of Radiation Protection and Research
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• 제20권2호
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• pp.129-136
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• 1995

중저준위 에너지 범위에서 방사선관리용측정기기의 에너지의존성에 대한 연구를 위하여 ISO에서 는 ISO표준 X선장을 발간하였으며, 또한 ANSI에서도 개인방사선측정 기기의 성능시험을 위하여 ANSI X선장을 제시 하였다. 그러나 X선 스펙트럼의 측정은 많은 시간이 소요될 뿐만 아니라 특히 측정 X선 스펙트럼에 unfolding이 수행된다면 매우 어려운 작업이 된다. 따라서 이론적인 방법에 의해 스펙트럼을 계산 예측하고 그 평균에너지 및 선량환산인자를 계산하는 것은 X선 실험제작에 필요한 시간, 노력 및 비용을 최소화 할 수 있을 것이다. 따라서 본 연구에서는 ISO Narrow series 및 ANSI X선장에 대하여 이론적으로 스펙트럼을 계산하였으며, 그에 의한 평균에너지와 선량당량혼산인자를 계산하였다. 본 계산에 의한 평균에너지 및 선량환산인자는 측정스펙트럼에 의한 ISO 및 X선장의 값과 잘 일치하였다.

• 한국자기공명학회논문지
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• 제15권2호
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• pp.137-145
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• 2011

Hsp33, a prokaryotic molecular chaperone, exerts holdase activity in response to oxidative stress. In this study, the stepwise conformational change of Hsp33 upon oxidation was monitored by NMR. In order to overcome its high molecular weight (33 kDa as a monomer and 66 kDa as a dimer), spectra were simplified using a selectively [$^{15}N$]His-labeled protein. All of the eight histidines were observed in the TROSY spectrum of the reduced Hsp33. Among them, three peaks showed dramatic resonance shifts dependent on the stepwise oxidation, indicating a remarkable conformational change. The results suggest that unfolding of the linker domain is associated with dimerization, but not entire region of the linker domain is unfolded.