• Archives of Pharmacal Research
• /
• 제12권4호
• /
• pp.289-294
• /
• 1989

A computer program which can differentiate compounds whose absorbance spectra are very similar was developed. The program. [SPECMAN PLUS], written in Pascal provides automated spectral comparison techniques, utilizing the values of Root Mean of Squares (RMS) of differences. This comparison routine of the program can deal with spectra of compounds different concentrations and different spectral recording resolutions. In addition, the program was designed applicable to any spectral data of digital form. The program was applied to the UV spectra of 13 pencillins and 5 cephalosporins, whose absorbance spectra are so similar. As a result, all compounds examined could be differentiated from each other.

• Bulletin of the Korean Chemical Society
• /
• 제32권9호
• /
• pp.3411-3420
• /
• 2011

Kinetic and structural studies have been carried out on the effects of meso-tetrakis(4-sulfonatophenyl)-porphyrin ($H_2TPPS_4$) as an anionic and meso-tetrakis(3-N-methyl-pyridyl)porphyrin ($H_2TMPYP$) as a cationic porphyrin with adenosine deaminase (ADA) in 25 mM citrate/phosphate buffer, pH = 4-8, at $37^{\circ}C$ using UVvis spectrophotometry, circular dichroism (CD), fluorescence spectrophotometry as well as molecular dynamics (MD) and molecular docking. Kinetic results showed that the two porphyrins are non-competitive inhibitors. Increasing pH, increases $K_I$ and cationic porphyrin has a higher $K_I$ and lower binding constant ($K_b$) at all pH ranges. Analyzing the secondary structure revealed that both ligands decrease the secondary structure and that the anionic porphyrin is more effective.

• 대한화학회지
• /
• 제7권4호
• /
• pp.280-282
• /
• 1963

Spectrophotometry에 의한 몰比法, 運續變化法 및 傾科比法으로 chloroform phase에 存在하는 Fe(III)-cupferrate의 組成을 決定하였는데, 그 황化合物은 325m$\mu$에서와 385m$\mu$에서 모두 $FeCupf_3^인 化學式을 갖는 것이었다.

• 한국해양학회지
• /
• 제15권1호
• /
• pp.66-70
• /
• 1980

A method for the determination of dissolved species of Cd, Co, Cu, Ni, Pb and Zn in sea water by flame atomic absorption spectrophotometry (AAS) is described. Prior to analysis by AAS, these elements are concentrated by coprecipitation with iron pyrrolidinedithiocarbamate at pH 3 because of matrix effects and their low concentration levels in sea water. The detection limits are 0.01, 0.04, 0.02, 0.05, 0.08, and 0.03$\mu\textrm{g}$/l, and the relative standard deviations are 1.0, 2.4, 1.3, 2.9, 2.0 and 2.9% for Cd, Co, Cu, Ni, Pb and Zn, respectively. The method is shown to be satisfactory in terms of recovery and precision for the determination of these metals in sea water.

• Asian-Australasian Journal of Animal Sciences
• /
• 제14권9호
• /
• pp.1321-1325
• /
• 2001

Color characteristics of pig ${\times}$ wild boar meat were determined with a fiber-optic spectrophotometer. The spectrophotometric characteristic of reduced-myoglobin observed after cutting immediately changed to the spectrophotometric characteristic of oxymyoglobin after 15 minutes of cutting. The spectrophotometry at 400 to 700 nm after 30, 45, 60, 90 and 120 minutes of cutting changed slightly. Compared with M. longissimus thoracis, M. rhomboideus had higher reflectance around 400 nm and from 650 to 1,100 nm and M. spinalis was lower in the visible light region after 60 minutes of cutting. The pig ${\times}$ wild boar meat was similar in reflectance shape with pork but was lower in intensity. The differences depended on the anatomical location. The M. rhomboideus from pig ${\times}$ wild boar had greatly lower reflectance than that from pig, the M. longissimus thoracis reflectance was lower, but M. spinalis reflectance hardly differed. These results showed that pig ${\times}$ wild boar meat had no special characteristic of blooming but had distinguishing characteristic of meat color among anatomical locations.

• 한국식품영양학회지
• /
• 제12권5호
• /
• pp.508-512
• /
• 1999

In order to qualify and quantify carotenoid pigment of pumpukin were analyzed by HPLC column chromatography and spectrophotometry. Eightyseven percent of total carotenoids in the pumpkin was in the fiber amounting 65.33mg%. however flesh and skin contained 6.61 and 3.31mg% respectvely. A silica gel column chromatography of the carotenoids produced 2 different peaks. The components eluted in peak II showed blue color when treated with hydrochloric acid. The fact suggested it contained epoxide and the component in peak II was a monol. By the separation with HPLC. $\alpha$ and $\beta$-carotene were the major compounds in the carotenoids. In fiber the total $\beta$-carotene content was 1.6 times higher than in the flesh and skin. The fiber contained less unidentified compounds than other part which contained 27.3~32.8%.

• 분석과학
• /
• 제8권4호
• /
• pp.461-464
• /
• 1995

Three wavelength spectrophotometry was used to determine the content of eugenol in Eugenia Caryophyllata Thunb. Using this method could effectively eliminate the deviation of background absorption caused by the change of concentration and the error of quantitative analysis caused by asymmetric peaks, and at the same time the leaning degree of base line was corrected. This method was simple the recovery ratio was 90.05%-116.94% and the coefficient of variation was 3.5%.

• 대한화학회지
• /
• 제7권3호
• /
• pp.197-202
• /
• 1963

The ${\alpha}-and\;{\beta}$-isomer of 4-nitroazoxybenzenes have been separated by liquid chromatography and their U.V. spectra were examined. The n${\to}{\pi}^{\ast}$ transition band of the compounds did not appear, likewise the cases of other compounds of the series. Transition band of the new isomer were as usual as those of other azoxy-compounds, whereas the ${\pi}{\to}{\pi}^{\ast}$ transition band of the other isomer which is reported in the literature shown peculier hypochromic shift and hypochromic effect. From the spectroscopic point of view it is very likely that the new isomer (m.p. $184-5^{\circ}C$) is ${\alpha}$-isomer and the other one (m.p. $152^{circ}C$) is ${\beta}$-isomer contrary to the literature.

• Elastomers and Composites
• /
• 제51권1호
• /
• pp.10-16
• /
• 2016

Nanosized palladium particles were synthesized using palladium(II) chloride, trisodium citrate dihydrate, and sodium borohydride under stirring condition. Nanosized palladium-graphene composites were prepared from palladium nanoparticles, and graphene was enclosed with polyallylamine under stirring condition for 1 h followed by ultrasonication for 3 h. Nanosized palladium-graphene composites were heated in an electric furnace at $700^{\circ}C$ for 2 h and characterized by X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. UV-vis spectrophotometry was used to evaluate the nanosized palladium-graphene composites as a catalyst in the photocatalytic degradation of various organic dyes such as methylene blue, methyl orange, rhodamine B, and brilliant green under ultraviolet light at 254 nm.

• Journal of Astronomy and Space Sciences
• /
• 제9권1호
• /
• pp.111-119
• /
• 1992

The IUE archival spectra of VV Cephei were collected to investigate the eclipse nature in the ultraviolet. The temperature of the B star has been determined, as approximately 30000K, based on the flux distributions during egress. Light curves of VV Cephei were reduced from the spectrophotometry of the IUE archival spectra. Three light curves at the center wavelengths of 2350 ${\AA}$, 2550 ${\AA}$ and 2850 ${\AA}$ have been analyzed by the modified Wilson and Devinney light curve program. The radii of the B star and M star were deduced to 0.05 and 0.22 of unit separation, respectively. The UV light curves show an evidence that the light was attenuated by the highly opaque atmosphere of the M star.