• Biomedical Science Letters
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• v.23 no.2
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• pp.64-72
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• 2017

The formation of brown colonies due to phenol oxidase activity on classic agar media containing natural material extracts of Helianthus annuus or on medium containing L-3,4-dihydroxyphenylalanine has been used to identify Cryptococcus species complex. In this study, various natural materials were used to develop a modified medium and to identify five major serotypes of Cryptococcus species complex. Serotypes A, D, and A/D were pigmented on medium using Perilla frutescens var. japonica Hara (PerJ agar) after a three-day incubation. Serotypes B and C were pigmented on PerJ agar after four- and five-day incubations, respectively. Growth time and pigmentation of the five serotypes occurred more rapidly on PerJ agar than on the other media. In addition, colony morphology, size, and pigmentation were specific by serotype. In conclusion, PerJ agar should be used in clinic settings to identify Cryptococcus species complex and its serotypes rapidly.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.147.1-147
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• 2003

Freesia, a member of the Iridaceae family, has fragant, tubular shaped flowers and is very popular ornamental plants in the world. Diseased freesia plants showing systemic leaf streak mosaic symptoms were collected from a cultivated farm in Kyonggi province, Korea in 2003, and its causal agents were investigated. Two viruses, Cucumber mosaic virus (Fr-CMV) and a potyvirus, were identified from the leaf tissues of the diseased freesia based on sequence analysis and host range tests. CMV-Fr could infect systemically on Chenopodium quinoa, C. amaranticolor, N. glutinosa, and N. benthamiana, and this biological property is distinguishable from ordinary strains of CMV. A filamentous potyvirus-shaped virus could not infect general indicator plants by mechanical inoculation. Single RT-PCR products was successfully amplified with a set of degenerate primers specific to the Potyvirus genus and total nucleic acids from the infected tissues, and was cloned into the pGEMT-Easy vector. Nucleotide sequences confirmed it belongs to the Potyvirus genus with either a new species or an isolate of Freesia mosaic virus (no information is available for the FrMV). This is the first report of FrMV in Korea and more characterizations of the two viruses are in progress.

• Korean Journal of Plant Resources
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• v.26 no.4
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• pp.463-490
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• 2013

This study was carried out to investigate the vascular plants of Namhan River. The period of survey was from March to November on 2012. Vascular plants based on voucher specimen were summarized as 430 taxa including 86 families, 250 genera, 383 species, 2 subspecies, 39 varieties and 6 forms. The rare plant species designated by Korea Forest Service were 13 taxa including Penthorum chinense Pursh, Polygonatum stenophyllum Maxim, and Sparganium erectum L., etc. And the endemic plant species were 8 taxa including Salix koriyanagi Kimura, Clematis trichotoma Nakai, and Carex gifuensis Franch., etc. Furthermore, 56 taxa were listed as specific plant species based on phytogeographical and based on the list of plants which have been approved for overseas delivery, 27 taxa were recorded in the investigated area. The naturalized plants were recorded as 58 taxa, and their Naturalization Ratio and Urbanization Index were recorded as 13.5%, and 18.1%, respectively. 430 taxa listed consists of 174 taxa(40.5%) of pasturing plant, 141 taxa(32.8%) of edible plants, 112 taxa(26.0%) of medicinal plants, 48 taxa(11.2%) of ornamental plants, 11 taxa(2.6%) of stain plants, 7 taxa(1.6%) of fiber plants, 4 taxa(0.9%) of timber plants, 2 taxa(0.5%) of industrial plants and 109 taxa(25.4%) of unknown plants.

• Journal of Environmental Impact Assessment
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• v.27 no.6
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• pp.521-547
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• 2018

To provide fundamental information to areas that lack data on the plant resources and the ecosystem, this study conducted a survey on the vascular plants in Mt. Jigisan in Hongseonggun. The vascular plants identified during the six-round field surveys were a total of 443 taxa: 96 families, 286 genera, 391 species, 7 subspecies, 40 varieties, 3 forms, and 2 hybrids. On floristic regional of Korea, Mt. Jigisan is deciduous broad-leaved and conifer-mixed forest which is south province of Korean peninsula. 4 taxa of Korean endemic plants such as Populus ${\times}$ tomentiglandulosa, Forsytsythia koreana, Forsytsythia ovate, and Iris rossii var. latifolia were collected. The vascular plants on the red list according to IUCN evaluation basis were found to be 6 taxa: Vulnerable (VU) species of Forsytsythia ovata, Least Conern (LC) species of Chionanthus retusus, Pseudoraphis ukishiba, and Cymbidium goeringii, and Not Evaluate (NE) species of Panax ginseng, and Fimbristylis verrucifera. The floristic regional indicator plants were found 27 taxa: 2 taxa of grade V, 3 taxa of grade IV, 6 taxa of grade III, 4 taxa of grade II, and 12 taxa of grade I. The specific distribution plants were found 27 taxa: 4 taxa of northern part of plants, 12 taxa of southern part of plants, 10 taxa of isolation distribution of plants, and 1 taxon of recently reported Viola betonicifolia var. albescens. The invasive alien plants were identified as 67 taxa. The naturalized index (NI) was 15.12%, and urbanization index (UI) was 20.9%, respectively.

• Journal of Ginseng Research
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• v.33 no.1
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• pp.55-58
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• 2009

Generally, the direct sequencing through PCR is faster, easier, cheaper, and more practical than clone sequencing. Frequently, standard PCR amplification is usually interpreted by mispriming internal or external regions of the target template. Normally, DNA fragments were eluted from the gel using Gel extraction kit and subjected to direct sequencing or cloning sequencing. Cloning sequencing has often troublesome and needs more time to analyze for many samples. Since touchdown (TD) PCR can generate sufficient and highly specific amplification, it reduces unwanted amplicon generation. Accordingly, TD PCR is a good method for direct sequencing due to amplifying wanted fragment. In plants the 5S-rRNA gene is separated by simple spacers. The 5S-rRNA gene sequence is very well-conserved between plant species while the spacer is species-specific. Therefore, the sequence has been used for phylogenetic studies and species identification. But frequent occurrences of spurious bands caused by complex genomes are encountered in the product spectrum of standard PCR amplification. In conclusion, the TD PCR method can be applied easily to amplify main 5S-rRNA and direct sequencing of panax ginseng cultivars.

• Korean Journal of Plant Taxonomy
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• v.40 no.2
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• pp.105-107
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• 2010

This study was carried out to find specific characters of the seed morphology and anatomy of Korean Eranthis in Ranunculaceae. As a result, E. byunsanensis differs with E. stellata on the basis of presence of unicellular hairs on the seed surface. E. stellata has no unicellular hairs on its seed surface. On the other hand, E. byunsanensis bears such unicellular hairs derived from epidermal cells of the seed surface. Besides other morphological characters used when it was published as a new species, the presence of unicellular hairs on the seed surface strongly supports it as an endemic species of Korea. In addition, to compare the seed morphology of E. pinnatifida and E. pungdoensis, for which mature seeds were not available at this time, an additional study should be conducted in near future.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.15 no.3
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• pp.55-66
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• 2012

This study was carried out to investigate vegetation of Rhododendron mucronulatum habitat in Mt. Biseul to recommend basic information for synecological characteristics and management methods. The survey was carried out from May to July, 2011 and totally 46 vegetation data including 42 families 93 genera 108 species 16 varieties and 5 forma were collected and analyzed. Among the investigated 129 taxa, the numbers of rare plant resources were summarized as 19 taxa; The rare plants designated by Korea Forest Service, the specific plants by floristic region and the endemic plants were listed as 3 taxa, 15 taxa and 4 taxa, respectively. Rhododendron mucronulatum habitat of Mt. Biseul was classified into 3 plant communities (Carex lanceolata-Rhododendron mucronulatum community, Potentilla dickinsii-Selaginella rossii community, Carex lanceolata-Quercus mongolica community). Carex lanceolata-Rhododendron mucronulatum community was subdivided into Tripterygium regelii subunit and Miscanthus sinensis for. purpurascens subunit by human interference degree. And synecology, syndynamics, synchorology of these plant communities were identified, and Site-species ordination analysis by Principal Coordinate Analysis (PCoA) reflected that human impact, soil moisture condition were main ecological factors determining the distribution pattern of classified plant communities. Therefore these plant communities correspond to quite distinctive 4 habitat types : unstable-dry type=Miscanthus sinensis for. purpurascens subunit, unstable-moderate type=Tripterygium regelii subunit, stable-dry type=Potentilla dickinsii-Selaginella rossii community, stable-moderate type=Carex lanceolata-Quercus mongolica community. Finally, through the vegetational diagnosis, proper management methods such as a limit on the access of visitors, planting of native woody plants after removing unwanted vines or grass were suggested.

• Journal of Microbiology and Biotechnology
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• v.31 no.9
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• pp.1218-1230
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• 2021

Cold-adapted plant growth-promoting bacteria (PGPB) with multiple functions are an important resource for microbial fertilizers with low-temperature application. In this study, culturable cold-adapted PGPB strains with nitrogen fixation and phosphorus solubilization abilities were isolated. They were screened from root and rhizosphere of four dominant grass species in nondegraded alpine grasslands of the Qilian Mountains, China. Their other growth-promoting characteristics, including secretion of indole-3-acetic acid (IAA), production of siderophores and ACC deaminase, and antifungal activity, were further studied by qualitative and quantitative methods. In addition, whether the PGPB strains could still exert plant growth-promoting activity at 4℃ was verified. The results showed that 67 isolates could maintain one or more growth-promoting traits at 4℃, and these isolates were defined as cold-adapted PGPB. They were divided into 8 genera by 16S rRNA gene sequencing and phylogenetic analysis, of which Pseudomonas (64.2%) and Serratia (13.4%) were the common dominant genera, and a few specific genera varied among the plant species. A test-tube culture showed that inoculation of Elymus nutans seedlings with cold-adapted PGPB possessing different functional characteristics had a significant growth-promoting effect under controlled low-temperature conditions, including the development of the roots and aboveground parts. Pearson correlation analysis revealed that different growth-promoting characteristics made different contributions to the development of the roots and aboveground parts. These cold-adapted PGPB can be used as excellent strain resources suitable for the near-natural restoration of degraded alpine grasslands or agriculture stock production in cold areas.

• Journal of Environmental Science International
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• v.21 no.8
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• pp.931-938
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• 2012

The effects were investigated of different lengths and numbers of slitwalls in drainless containers on growth and change in soil moisture volumes on the growth of Diffenbachia amoena 'Marianne' and Begonia rex. Drainless containers filled with amended soil, with square shape ($240mm{\times}240mm$) were used, as well as three different sets of slitwalls (2, 4 or 8, respectively) in addition to non-slitwall containers. Two indoor foliage plants were grown in slitwall containers in randomized blocks with 3 replications in greenhouse conditions, from March to September, 2009. Soil moisture volumes per container were measured by weighing containers every 2 hours during the day. The change in soil moisture volumes showed considerable differences among slitwalls tested in comparison to control containers before and after twice-weekly irrigation. Particularly, the differences in the S2 (195mm, slitwall 2) containers were significantly greater than other containers tested. For Diffenbachia amoena 'Marianne', plant height, length of leaf, dry weight and fresh weight were higher with S2 containers than with those grown in other containers tested. The Begonia rex with the best quality in terms of plant height, length of leaf and width of leaf was grown in S8 (360mm, slitwall 8) containers. Particularly, statistical analysis has indicated that shoot fresh weights of Begonia rex grown in S8 were 3-fold higher than those grown in CS8 containers. The different results obtained within the two species led us to hypothesize a species-specific influence on indoor foliage plant performance. However, plants of both species grown in slitwall containers showed good results compared with plants grown in non-slitwall containers.

• Korean Journal of Environmental Agriculture
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• v.34 no.4
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• pp.260-267
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• 2015

BACKGROUND: The current study was conducted to examine the species specific accumulation of Cd and Pb in 11 crop species (Soybean, Sesame, Corn, Polished rice, Carrot, Potato, Garlic, Spring onion, Chinese leek, Red pepper, Eggplant), through cultivating them under the same condition with metal contaminated soils.METHODS AND RESULTS: Eleven crop species were cultivated in three different soils contaminated with Cd and Pb and harvested. Edible parts of each crop was pretreated and analyzed to determine Cd and Pb concentrations, and subsequently bioconcentration factors (BCFs) were calculated. In general, the crops of which seeds are used as food showed high concentrations of both Cd and Pb. For instance, Cd concentrations in crops cultivated in Soil A was in the order of soybean (0.432 mg kg^-1) > sesame (0.385) > polished rice (0.176) > carrot (0.116) > corn (0.060) > red pepper > (0.047) > potato (0.044) > egg plant (0.025) > garlic (0.023) > spring onion (0.016) > Chinese leek (0.011). BCFs showed the same order.CONCLUSION: From this study, it can be conclude that seeds plants should not be cultivated in Cd and Pb contaminated soils to secure food safety from metal contaminated soils.