• Proceedings of the National Institute of Ecology of the Republic of Korea
• /
• 제3권4호
• /
• pp.212-220
• /
• 2022

DNA markers have been studied and used intensively to identify plant species based on molecular approaches. The genus Medicago belongs to the family Fabaceae and contains 87 species distributed from the Mediterranean to central Asia. Five species of Medicago are known to be distributed in South Korea; however, their morphological characteristics alone cannot distinguish the species. In this study, we analyzed the phylogenetic relationships using collected five species of Medicago from South Korea and 44 taxa nucleotide information from NCBI. The constructed phylogenetic tree using gibberellin 3-oxidase 1 and tRNA^Lys (UUU) to maturase K gene sequences showed the monophyly of the genus Medicago, with five species each forming a single clade. These results suggest that there are five species of Medicago distributed in South Korea. In addition, we designed polymerase chain reaction primers for species-specific detection of Medicago by comparing the plastid sequences. The accuracy of the designed primer pairs was confirmed for each Medicago species. The findings of this study provide efficient and novel species identification methods for Medicago, which will assist in the identification of wild plants for the management of alien species and living modified organisms.

• 한국자원식물학회지
• /
• 제14권3호
• /
• pp.235-240
• /
• 2001

Polyphenol 화합물이 다량 함유된 식물종의 유연관계 분석이나 형질전환 유전자 확인 등을 위해 PCR을 이용할 경우 다량의 재료로부터 신속 간편하게 분리한 DNA를 이용할 수 있는 조건을 설정 하였다. 폴리페놀 함량이 높은 포도, 사과, 복분자와 같은 과수류에서 간편법에 의해 추출된 DNA를 이용한 PCR 반응액에 2%의 BLOTTO를 첨가함으로서 DNA의 재현적 증폭이 가능하였다. 간편 추출 DNA를 이용한 PCR에서 의 BLOTTO효과는 primer, 품종, 식물종에 관계없이 일반적으로 발현되었다. 상추의 형질전환 유전자 검색을 위한 PCR에서 도 BLOTTO 효과가 확인되었다. 따라서 PCR 반응액에 2% BLOTTO를 첨가하면 간편 법 에 의해 추출된 polyphenol 화합물 고함유 식물종의 DNA를 이용하여서도 PCR에 의한 유전배경 및 특정 유전자의 대량 신속 분석이 가능할 것이다.

• 한국자원식물학회지
• /
• 제17권2호
• /
• pp.107-115
• /
• 2004

참다래 육종을 위한 종 분류와 분자 표지인자로서 유용하게 이용될 수 있는 primer를 개발하기 위하여 참다래 genome 특이 반복 염기서열로부터 19∼20base 크기로 18개의 primer를 제작하여 kiwifruit target primer(KT primer)라 명명하였으며, 동아시아 지역에서 수집된 7종 22계통의 다래나무 속 식물을 이용하여 활용 가능성 을 조사하였다. 유연관계 분석을 위하여 7개의 primer가 선발되었으며, 이를 이용한 RAPD 결과 크게 2개의 군으로 나뉘어 졌다. 제 1 군(A. arguta, A. melanandra, A. kolumikta와 A. marcrosperma)은 주로 과실에 전혀 털이 없으며 잎에는 털이 전혀 없거나 어렸을 때 극소량의 연모가 있다가 없어지는 그룹으로서 Leiocarpae 절에 속하였다. 제 2 군(A. chinensis, A. deliciosa 및 A. eriantha)은 어린 과실에서는 털이 많았다가 성숙하면서 털이 없어지는 계통 및 잎과 줄기에 털이 아주 많거나 조밀한 솜털이 있는 그룹으로서 Stellatae절에 속하였다. 제 2 군은 Stellatae 절에서도 Pefectae 아절에 속하는 것으로 A. chinensis, A. deliciosa 및 A. eriantha가 포함되었으며, 다시 A. chinensis와 A. deliciosa를 포함하는 그룹과 A. eriantha 등 2개의 그룹으로 나뉘어졌다. 같은 부모로부터 유래된 것으로 알려진 A. chinensis와 A. deliciosa는 80％의 유사도에서 두 개의 그룹으로 나뉘어졌다. 또한 PCR 결과 A. deliciosa 종 및 헤이워드와 토무리 계통 특이 밴드가 KT12F와 KT6F에서 나타났으며, 유전양상 분석에서 KT7F와 KT12F가 유용하였다. 본 연구 결과 KT primer는 참다래의 유전양상 분석과 특이한 유전양상을 나타내는 개체선발 및 도태에 유용하게 이용될 수 있고, 또한 참다래 육종 효율향상에 많은 도움을 줄 수 있다고 판단되었다.

• 한국지하수토양환경학회지:지하수토양환경
• /
• 제15권3호
• /
• pp.61-68
• /
• 2010

Phytoextraction, one type of phytoremediation processes, has been widely used in the removal of heavy metals from polluted soil. This paper reviewed literature on metal uptake by plants and characterized the metal uptake by types of metals (Zn, Cu, Pb, Cd, and As), plant species, initial metal concentrations in soil and the distribution of metals in different parts of plants. The potential of metal accumulation and transport by plants was closely related to plants species, types of metals, and initial metal concentrations in soil. The plants belonging to Brassicaceae, Solanaceae, Poaceae, and Convolvulaceae families have shown the high potential capacity of Cd accumulation. The Gentianaceae, Euphorbiaceae, and Polygonaceae families have exhibited relatively high Pb uptake potential while the Pteridaceae and Cyperaceae families have shown relatively high Zn uptake potential. The Pteridaceae family could uptake a remarkably high amount of As compared with other plant families. The potential metal accumulation per plant biomass has increased with increasing initial metal concentration in soil up to a certain level and then decreased for Cd and Zn. For As, only Pteris vittata had a linear relationship between initial concentration in soil and potential of metal uptake. However, a meaningful relationship for Pb was not found in this study. Generally, the plants having high metal uptake potential for Cd or Pb mainly accumulated the metal in their roots. However, the Euphorbiaceae family has accumulated more than 80% of Pb in shoot. Zn has evenly accumulated in roots and stems except for the plants belonging to the Polygonaceae and Rosaceae families which accumulated Zn in their leaves. The Pteridaceae family has accumulated a higher amount of As in leaves than roots. The types of metals, plant species, and initial metal concentration in soil influence the metal uptake by plants. It is important to select site-specific plant species for effective removal of metals in soil. Therefore, this study may provide useful and beneficial information on metal accumulation by plants for the in situ phytoremediation.

• Journal of Forest and Environmental Science
• /
• 제28권4호
• /
• pp.247-253
• /
• 2012

The purpose of this study was to conduct phenology monitoring of forest plant species in Mt. JuWang National Park, thereby establish long-term prediction and management system for species susceptible to climate change, and utilize the result as basic materials necessary for conservation of plant genetic resources in accordance with changes in their growth environment. Global Positioning System coordinates were marked on each indicator species and a specific number ticket was provided to each plant. Changes in their blooming time, time of blossoms falling, time of leaves bursting into life, and time of leaves turning, and time of leaves falling were recorded. Investigation was made once per week from April 10 in 2010 to November 30 in 2011 except for the time period between July and August when investigation was made biweekly. The investigated plants concerned 12 kinds-nine species of trees and three kinds of herbs. According to the result of the penology monitoring of Mt. JuWang National Park, their time of leaves bursting into life, time of leaves turning, and time of leaves falling were largely earlier in 2011 than in 2010. However, it is hard to say that it is due to the factor of climate change. Long-term collection of climate data and continuous monitoring of plant phenology are considered necessary in order to examine correlation between climate change and seasonal change patterns of plants.

• 한국자원식물학회지
• /
• 제35권2호
• /
• pp.317-345
• /
• 2022

본 연구는 산림 생물다양성 특정 지역인 강원도 정선군 정선읍 여탄리 풍혈을 대상으로 기후변화에 취약한 북방계식물 등의 보전을 위한 기초자료를 활용하기 위하여 조사를 수행하였다. 조사는 풍혈 바람구멍을 기분으로 풍혈의 내부 625 m²와 외부 6,925 m²를 나누어 조사하였으며 총7,550 m²를 조사지역으로 설정하였고, 2020년 6월부터 2021년 11월까지 계절별로 각 1~2회씩총9회에 걸쳐 수행하였다. 여탄리 풍혈의 관속식물은 84과 203속 296종 6아종 27변종 2품종 총331분류군으로 확인되었다. 이는 우리나라 관속식물 4,724종의 약 7%로 나타났다. 한반도 특산식물은 버들개회나무 등 19분류군, IUCN 지정 희귀식물은 11분류군으로 확인되었다. 멸종위기등급(CR)은 산작약 1분류군, 위기등급(EN)은 산개나리와 복사앵도나무 2분류군, 취약등급(VU)은 세잎승마와 백작약 2분류군, 약관심등급(LC)은 등칡 등 4분류군 등이 확인되었다. 식물구계학적 특정식물은 총78분류군이 조사되었다. V등급은 산작약과 갈퀴아재비 2분류군, IV등급은 도깨비부채 등 14분류군, III등급은 눈개승마 등 21분류군으로 조사되었다. 석회암지대 식물은 참골담초 등 19분류군이 확인되었다. 외래식물은 서양민들레 등 20분류군이 확인되었고, 귀화율 6.04%와 도시화율 5.33%로 산출되었다. 본 연구대상지인 정선 여탄리 풍혈의 식물지리학적 특정식물은 버들개회나무와 꽃개회나무 2분류군으로 파악되었다.

• 한국환경복원기술학회지
• /
• 제19권2호
• /
• pp.19-39
• /
• 2016

This study was carried out to investigated the flora of Naknam-Jeongmaek, Korea. The survey sites were Gilmajae, Mt. Muryang, Mt. Yeohang, Mt. Muhak, Mt. Cheonju and Mt. Sineo. The flora were summarized, numbering 666 taxa including 112 families, 357 genera, 574 species, 4 subspecies, 73 varieties and 15 forms. The threatened species was Aconitum austrokoreense. The rare plants were 12 taxa including Asarum maculatum, Wikstroemia ganpi, Viola albida, Tricyrtis macropoda and so forth. The Korean endemic plants were 19 taxa including Salix koriyanagi, Thalictrum actaefolium var. brevistylum, Weigela subsessilis, Hosta minor and so forth. The specific plants by floristic region were 81 taxa including 38 taxa of grade I, 19 taxa of grade II, 16 taxa of grade III, 7 taxa of grade IV and 1 taxa of grade V. The naturalized plants were 32 taxa including Rumex crispus, Physalis angulata, Erechtites hieracifolia, Taraxacum officinale and so forth. The invasive alien plants were Ambrosia artemisiifolia and Aster pilosus.

• 한국약용작물학회지
• /
• 제22권4호
• /
• pp.301-305
• /
• 2014

This study was carried out to investigate the vegetation and habitat environment of Sageretia thea which is distributed in Jeju island, Korea. Sageretia thea were mainly distributed to the west area in Jeju island. Soil pH and EC of Sageretia thea habitat were 5.8 and $0.34dS{\cdot}m^{-1}$. The contents of organic matter, available phosphate, exchangeable potassium, exchangeable sodium, exchangeable magnesium and exchangeable calcium were 15.27%, $13.6mgkg^{-1}$, $0.27cmol_+kg^{-1}$, $1.3cmol_+kg^{-1}$, $1.7cmol_+kg^{-1}$, $4.9cmol_+kg^{-1}$, respectively. Thirty one taxa including 25 families, 31 genus, 27 species and 4 varieties were identified surrounding Sageretia thea habitat. Specific plant species were 1 taxon in III grade, 1 taxon in I grade and 3 taxa in I grade.

• Journal of Microbiology and Biotechnology
• /
• 제12권6호
• /
• pp.989-992
• /
• 2002

Sweet persimmon (Diospyros kaki Thunb.) is widely cultivated in the southern part of Korea and its cultivation is increasing. However, anthracnose disease caused by Colletotricuhum species is one of the major hinderances to the cultivation and production of sweet persimmon. Therefore, in the current study, PCR was used to specifically detect Colletotrichum spp., based on the sequences of the ITS II regions in the rDNA. Using the sequence data, CO-1 was designated to detect Colletotrichum together the with ITS 4 primer. The result showed that a single segment of ca. 500 bp was observed only in Colletotrichum, but not in any other fungal and bacterial isolates. The annealing temperatures and template DNA quantites were also investigated to identify optimal conditions for detection. Using these species-specific primers, a unique band was obtained at annealing temperatures ranging from $55^{\circ}C\;and\;61^{\circ}C$ and template DNA levels from 10 pg- $10{\mu}g$.

• 한국환경복원기술학회지
• /
• 제20권5호
• /
• pp.15-41
• /
• 2017

The purpose of this study is to present the basic data for conservation of forest ecosystem by surveying and analysing the flora distributed in Nakdong-Jeongmaek Mountains, Korea. The numbers of flora were 901 taxa including 116 families, 433 genera, 790 species, 4 subspecies, 90 varieties, 15 forms and 2 hybrids. The threatened species was Pedicularis ishidoyana. The rare plants were 33 taxa including Pseudostellaria sylvatica, Cimicifuga heracleifolia var. bifida, Isopyrum mandshuricum, Paeonia japonica, Syringa patula var. kamibayshii, Lilium distichum, Iris odaesanensis and so forth. The Korean endemic plants were 35 taxa including Salix koriyanagi, Thalictrum actaefolium var. brevistylum, Corydalis maculata, Lespedeza maritima, Cirsium setidens, Carex okamotoi and so forth. The specific plants by floristic region were 155 taxa including 55 taxa of grade I, 41 taxa of grade II, 36 taxa of grade III, 18 taxa of grade IV and 5 taxa of grade V. The naturalized plants were 62 taxa including Rumex obtusifolius, Amaranthus lividus, Medicago sativa, Geranium carolinianum, Malva sylvestris var. mauritiana, Foeniculum vulgare, Sonchus oleraceus, Tradescantia reflexa and so forth. The invasive alien plants were 5 taxa including Rumex acetocella, Solanum carolinense, Ambrosia artemisiifolia, Aster pilosus and Lactuca scariola.