• The Plant Pathology Journal
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• 제31권1호
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• pp.90-96
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• 2015

Garlic generally becomes coinfected with several types of viruses belonging to the Potyvirus, Carlavirus, and Allexivirus genera. These viruses produce characteristically similar symptoms, they cannot be easily identified by electron microscopy (EM) or immunological detection methods, and they are currently widespread around the world, thereby affecting crop yields and crop quality adversely. For the early and reliable detection of garlic viruses, virus-specific sets of primers, including species-specific and genus-specific primers were designed. To effectively detect the twelve different types of garlic viruses, primer mixtures were tested and divided into two independent sets for multiplex polymerase chain reaction (PCR). The multiplex PCR assays were able to detect specific targets up to the similar dilution series with monoplex reverse transcription (RT)-PCR. Seventy-two field samples collected by the Gyeongbuk Agricultural Technology Administration were analyzed by multiplex RT-PCR. All seventy two samples were infected with at least one virus, and the coinfection rate was 78%. We conclude that the simultaneous detection system developed in this study can effectively detect and differentiate mixed viral infections in garlic.

• 식물분류학회지
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• 제39권3호
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• pp.199-215
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• 2009

벼속 17 분류군 엽신의 식물규소체에 대해 주사전자현미경의 후반사전자상을 사용하여 조사하고 검색표와 기재를 실시하였다. 검색표는 향축면 및 배축면에 대해 중륵위와 수적세포 규소괴, 유두상돌기, 유혁모, 대모, 미모, 기공장치 등 유래의 식물규소체의 특징에 따라 작성되었으며, 종 수준의 동정이 가능하였다. 본 속 식물의 상세한 규소체 기재와 함께 제공된 후반사전자상 사진 그리고 검색표는 식물의 동정과 고고학적 잔존물에서 출토된 식물규소체를 동정하는데 대조 자료로 사용될 것으로 평가된다.

• Mycobiology
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• 제49권3호
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• pp.235-248
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• 2021

This study aimed to understand whether the geo-ecological segregation of native plant species affects the root-associated fungal community. Rhizoplane (RP) and rhizosphere (RS) fungal microbiota of Sedum takesimense native to three geographically segregated coastal regions (volcanic ocean islands) were analyzed using culture-independent methods: 568,507 quality sequences, 1399 operational taxonomic units, five phyla, and 181 genera were obtained. Across all regions, significant differences in the phyla distribution and ratio were confirmed. The Chao's richness value was greater for RS than for RP, and this variance coincided with the number of genera. In contrast, the dominance of specific genera in the RS (Simpson value) was lower than the RP at all sites. The taxonomic identity of most fungal species (95%) closely interacting with the common host plant was different. Meanwhile, a considerable number of RP only residing fungal genera were thought to have close interdependency on their host halophyte. Among these, Metarhizium was the sole genus common to all sites. These suggest that the relationship between potential symbiotic fungi and their host halophyte species evolved with a regional dependency, in the same halophyte species, and of the same natural habitat (volcanic islands); further, the fungal community differenced in distinct geographical regions. Importantly, geographical segregation should be accounted for in national culture collections, based on taxonomical uniqueness.

• Journal of Plant Biotechnology
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• 제47권2호
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• pp.131-140
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• 2020

Solanum stoloniferum은 가지과에 속하는 4배체 감자 야생종 중의 하나로 감자 육종에서 다양한 병원균에 대한 저항성으로 인하여 좋은 재료로 활용되고 있다. 하지만, 감자와의 생식적 장벽으로 인하여 감자와 직접적인 교배를 통해 육종을 할 수 없어 이를 극복하기 위해 체세포 융합 등의 방법이 이용될 수 있다. 세포 융합 이후에는 분자마커를 이용하여 적합한 융합체 선발이 필요한데 이를 위해 본 연구에서는 S. stoloniferum 특이적 마커를 개발하기 위하여 S. stoloniferum의 엽록체 전장 유전체 정보를 분석하고 이를 기반으로 한 마커를 개발하였다. S. stoloniferum의 cpDNA 총 길이는 155,567 bp이고, 6개의 다른 Solanum 종과의 비교를 통해 S. stoloniferum가 S. berthaultii와 가장 가까운 유연관계인 것을 확인하였다. 다섯 종의 Solanum과의 엽록체 전장 유전체 다중 정렬에서는 S. stoloniferum 특이적인 6개의 InDel과 39개의 SNP를 구명하였으며, 이 정보를 이용하여 최종적으로 네개의 S. stoloniferum 특이적인 PCR 기반의 분자마커를 개발하였다. 이 마커들은 적절한 체세포 융합체를 선발하고 S. stoloniferum을 이용한 감자 품종 육성에 기여할 수 있을 것이다.

• Journal of Plant Biotechnology
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• 제47권2호
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• pp.141-149
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• 2020

Solanum hougasii는 감자 야생종 중의 하나로 다양한 종류의 병원균에 대해 저항성을 가지고 있어 감자 육종에서 중요한 재료로 이용되고 있다. S. hougasii는 이질6배체이나 4배체인 감자와 EBN이 4로 같아 직접적인 교배로 육종에 활용될 수 있다. 본 연구에서는 NGS 기술에 의해 완성된 S. hougasii의 엽록체 전장 유전체(cpDNA)와 이를 다른 Solanum종과의 비교를 통해 개발한 분자마커에 대해 보고하였다. S. hougasii의 전체 cpDNA의 크기는 155,549 bp였으며 그 구조는 다른 Solanum 종과 매우 유사하였다. S. hougasii의 cpDNA와 가지과에 속하는 10개 종의 cpDNA 코딩서열을 이용하여 분석한 계통수에서는 S. hougasii와 S. stoloniferum이 거의 동일한 유전체 구성을 보였으며, 다음으로 S. berthaultii 및 S. tuberosum과 유연관계가 가까운 것으로 확인되었다. S. hougasii와 다른 다섯 종의 Solanum과의 전체 cpDNA 다중 정렬을 통해 S. hougasii 특이적인 다섯 개의 InDel과 43개의 SNP 영역을 구명하였으며 이를 기반으로 최종적으로 PCR을 기반으로 한 네 개의 S. hougasii 특이적 마커를 개발하였다. 본 연구의 결과는 Solanum 종들을 대상으로 한 조금 더 세부적인 진화적 그리고 육종적 측면에서의 연구에 기여를 할 수 있을 것이다.

• 한국환경과학회지
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• 제18권6호
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• pp.633-644
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• 2009

The vascular plants in the Gyeryongsan National Park were listed 227 taxa; 71 families, 148 genera, 196 species, 1 subspecies, 26 varieties and 4 forms, Among them, trees and herbs were 103 taxa (45.4%), 124 taxa (54.6%), respectively. The rare plants were identified 6 taxa such as Paeonia obovata (Legal protection species), Viola albida, Acanthopanax chiisanensis, Lilium distichum, Lilium callosum and Goodyera schlechtendaliana by the Korea Forest Service. Based on the list of Korean endemic plants, 8 taxa were recorded; Cephalotaxus koreana, Pseudostellaria multiflora, Thalictrum uchiyamai, Indigofera koreana, Acanthopanax chiisanensis, Asperula lasiantha, Weigela subsessilis and Carex okamotoi. Based on the list of approved for delivering overseas of plants, 6 taxa were recorded; Viola albida, Acanthopanax chiisanensis, Asperula lasiantha, Carex okamotoi, Lilium distichum and Lilium callosum. Specific plant species by floral region were total 21 taxa (9.3%); Paeonia obovata in class V, 5 taxa (Betula davurica, Acer palmatum, Asperula lasiantha, Lonicera subhispida, Phaenosperma globosa) in class III, 4 taxa (Viola orientalis, Acanthopanax chiisanensis, Ostericum melanotilingia, Lilium distichum) in class II and 11 taxa (Cephalotaxus koreana, Betula schmidtii, Quercus variabilis, Aphananthe aspera, Ilex macropoda, Meliosma oldhamii, Tilia amurensis, Lilium callosum, Erythronium japonicum, Polygonatum inflatum, Hosta capitata) in class I. It is important that relationship between national and local government will be needed for preservation of natural environment of Gyeryongsan National Park. As well, a long time of monitering and systematical management counterplan will be required.

• 한국환경과학회지
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• 제20권9호
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• pp.1125-1140
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• 2011

According to previous research on evaluating vegetative value of wetland and RAM evaluation targeting the man-made wetland in Gunsan, Gunsan Reservoir and Gongchang Reservoir had been found to have satisfactory results while Anjeong Reservoir and Changan Reservoir had been found to have unsatisfactory results. Aimed at those reservoirs, a vegetation survey was conducted to analyze differences in terms of flora and growth habit. As for the flora, Gunsan Reservoir ranked first with 433 kinds of plants, followed by Gongchang Reservoir with 306, Changan Reservoir with 176 and Anjeong Reservoir with 167. As for specific plant species by floristic region, it was identified that Gunsan Reservoir had 18 species, larger than other wetlands and also, it had more species of naturalized plants than others. This phenomenon is related to various wetland environments resulted from wider area of Gunsan Reservoir. In the case of dormancy form, hemicryptophytes were mainly distributed in Gunsan Reservoir and Gongchang Reservoir while annual plants were mainly distributed in Anjeong Reservoir and Changan Reservoir with heavy disturbance.

• 한국환경복원기술학회지
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• 제10권2호
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• pp.44-53
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• 2007

The vascular plants of the studied area in the Gyehwado was listed 378 taxa; 99 families, 259 genera, 322 species, 1 subspecies, 50 varieties and 5 forms. In this, Woody plants were 130 taxa (34.4%), Herbaceous plants were 248 taxa (65.6%) of all 378 taxa vascular plants. Pteridophyta were 7 families, 9 genera, 11 taxa (2.9%), Gymnospermae were 4 families, 5 genera, 9 taxa (2.4%) and Angiospermae were 88 families, 245 genera, 358 taxa (97.7%). Angiospermae in this, Monocotyledoneae were 10 families, 45 genera, 60 taxa (16.8%) and Dicotyledoneae were 78 families, 200 genera, 298 taxa (83.2%). Based on the list of the rare plants by the Forest Research Institute, recorded in the studied areas; Phacelurus latifolius (Preservation priority order; No. 194), Tricyrtis dilatata (No. 97), Aristolochia contorta (No. 151) and based on the list of Korean endemic plants, 6 taxa were recorded; Cephalotaxus koreana, Populus tomentiglandulosa, Carpinus coreana, Indigofera koreana, Forsythia koreana, Weigela subsessilis. Specific plant species by floral region were total 30 taxa; Wistaria floribunda in class IV, 5 taxa (Ligustrum ovalifolium, Callicarpa mollis, Lonicera subhispida, etc.) in class III, 24 taxa (Cephalotaxus koreana, Aristolochia contorta, Grewia biloba var. parviflora, etc.) in class I. The naturalized plants in this site were 12 families, 27 genera, 34 species, 2 varieties, 36 taxa and naturalization rate was 9.5% of all 378 taxa vascular plants.

• 환경영향평가
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• 제23권2호
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• pp.119-136
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• 2014

This work aims to select the potentially vulnerable plant species against climate change at alpine and subalpine belts of Mts. Sorak, Jiri, and Halla, from central, southern, southern insular high mountains of the Korean Peninsula, respectively. The selection of global warming related vulnerable plants were performed by adapting various criteria, such as flora, endemicity, rarity, floristically specific and valuable species, species composition at mountain summits, horizontal and vertical ranges of individual species, and their distributional pattern in the Korean Peninsula. Line and quadrat field surveys along the major trails from all directions at height above 1,500 meters above sea level of Mts, Sorak, Jiri and Halla were conducted each year during spring, summer, and autumn from 2010 to 2011. Based upon above mentioned eight criteria, high level of climate change related potentially vulnerable arboral plants, such as Rhododendron aureum, Taxus caespitosa, Pinus pumila, Oplopanax elatus, Vaccinium uliginosum, and Thuja koraiensis are noticed from at subalpine belt of Mt. Sorak. Species of Abies koreana, Rhododendron tschonoskii, Oplopanax elatus, Taxus cuspidata, Picea jezoensis, and Juniperus chinensis var. sargentii belong to climate change concerned vulnerable species at subalpine belt of Mt. Jiri. High level of climate change related species vulnerability is found at alpine and subalpine belts of Mt. Halla from Diapensia lapponica var. obovata, Salix blinii, Empetrum nigrum var. japonicum, Vaccinium uliginosum, Juniperus chinensis var. sargentii, Taxus cuspidata, Rhamnus taquetii, Abies koreana, Hugeria japonica, Prunus buergeriana, and Berberis amurensis var. quelpartensis. Countermeasures to save the global warming vulnerable plants in situ are required.

• The Plant Pathology Journal
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• 제25권3호
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• pp.247-255
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• 2009

Root-knot nematode species, such as Meloidogyne hapla, M. incognita, M. arenaria, and M. javanica are the most economically notorious nematode pests, causing serious damage to a variety of crops throughout the world. In this study, DNA sequence analyses were performed on the D3 expansion segment of the 28S gene in the ribosomal DNA in an effort to characterize genetic variations in the three Meloidogyne species obtained from Korea and four species from the United States. Further, PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism), SCAR (Sequence Characterized Amplified Region) PCR and RAPD (Randomly Amplified Polymorphic DNA) were also utilized to develop methods for the accurate and rapid species identification of the root-knot nematode species. In the sequence analysis of the D3 expansion segment, only a few nucleotide sequence variations were detected among M. incognita, M. arenaria, and M, javanica, but not M. hapla. As a result of our haplotype analysis, haplotype 5 was shown to be common in M. arenaria, M. incognita, M. javanica, but not in the facultatively parthenogenetic species, M. hapla. PCR-RFLP analysis involving the amplification of the mitochondrial COII and large ribosomal RNA (lrRNA) regions yielded one distinct amplicon for M. hapla at 500 bp, thereby enabling us to distinguish M. hapla from M. incognita, M. arenaria, and M. javanica reproduced via obligate mitotic parthenogenesis. SCAR markers were used to successfully identify the four tested root-knot nematode species. Furthermore, newly attempted RAPD primers for some available root-knot nematodes also provided some species-specific amplification patterns that could also be used to distinguish among root-knot nematode species for quarantine purposes.