The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Development of Multiplex RT-PCR for Simultaneous Detection of Garlic Viruses and the Incidence of Garlic Viral Disease in Garlic Genetic Resources

  • Nam, Moon (School of Applied Biosciences, Kyungpook National University) ;
  • Lee, Yeong-Hoon (Department of Functional Crop, National Institute of Crop Science) ;
  • Park, Chung Youl (School of Applied Biosciences, Kyungpook National University) ;
  • Lee, Min-A (School of Applied Biosciences, Kyungpook National University) ;
  • Bae, Yang-Soo (School of Applied Biosciences, Kyungpook National University) ;
  • Lim, Seungmo (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Joong Hwan (Institute for Bioresources Research, Gyeongsangbuk-do Agricultural Research and Extension Services) ;
  • Moon, Jae Sun (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Su-Heon (School of Applied Biosciences, Kyungpook National University)
  • Received : 2014.10.28
  • Accepted : 2014.11.16
  • Published : 2015.03.01
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Abstract

Garlic generally becomes coinfected with several types of viruses belonging to the Potyvirus, Carlavirus, and Allexivirus genera. These viruses produce characteristically similar symptoms, they cannot be easily identified by electron microscopy (EM) or immunological detection methods, and they are currently widespread around the world, thereby affecting crop yields and crop quality adversely. For the early and reliable detection of garlic viruses, virus-specific sets of primers, including species-specific and genus-specific primers were designed. To effectively detect the twelve different types of garlic viruses, primer mixtures were tested and divided into two independent sets for multiplex polymerase chain reaction (PCR). The multiplex PCR assays were able to detect specific targets up to the similar dilution series with monoplex reverse transcription (RT)-PCR. Seventy-two field samples collected by the Gyeongbuk Agricultural Technology Administration were analyzed by multiplex RT-PCR. All seventy two samples were infected with at least one virus, and the coinfection rate was 78%. We conclude that the simultaneous detection system developed in this study can effectively detect and differentiate mixed viral infections in garlic.

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