• Korean journal of applied entomology
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• v.54 no.3
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• pp.271-274
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• 2015

The genus Italochrysa Principi in Korea has been recorded with only one species, I. japonica (McLachlan) so far. Here we report that Italochrysa nigrovenosa Kuwayama is recognized for the first time in Korea. Specific description, a key based on diagnostic characters, and adult and genital images of the species are provided.

• Animal Systematics, Evolution and Diversity
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• v.36 no.3
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• pp.240-250
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• 2020

Member of the genus Tobrilus Andrássy, 1959, which belongs to Tobrilidae Filipjev, 1918, are known as free-living nematodes in freshwater habitat. This genus was previously unknown from Korea. Five Tobrilus species are here reported for the first time from Korea: Tobrilus aberrans (Schneider, 1925), Tobrilus diversipapillatus (Daday, 1905), Tobrilus gracilis (Bastian, 1865), Tobrilus longus (Leidy, 1851), and Tobrilus wesenbergi (Micoletzky, 1925). Specimens were collected from sediments of the Nakdong River in Korea. Morphological characters and measurements of the specimens generally agree with the original descriptions of Tobrilus species, except for some differences that can be attributed to intraspecific variation among populations(e.g., nerve ring position [% pharynx] and reproductive length). Each species can be distinguished from other members of the genus by specific characters (e.g., cephalic setae length and position, buccal cavity and pocket shape, vulva position, degree of development of reproductive system, and tail length and shape). Here, five species in the genus Tobrilus are fully redescribed and illustrated using optical microscopy images. DNA barcode sequence information (the D2-D3 region of 28S rDNA) is also provided for molecular species identification.

• Korean Journal of Plant Taxonomy
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• v.53 no.3
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• pp.201-212
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• 2023

Parasenecio pentaphylla, a new species of the genus Parasenecio from Mt. Ilwol-san, Ilwol-myeon, Yeongyang-gun, Gyeongsangbuk-do, Korea, is described and illustrated here. In Korea, this species has been recognized as P. adenostyloides, known to be a Japanese endemic species. However, it is clearly distinguished from P. adenostyloides by its involucre shape, the number of phyllaries, the number of florets per capitulum, and the ratio of the tube length to the throat length. A molecular phylogenetic analysis using the Internal Transcribed Spacer region also clearly indicated that P. pentaphyllaria was genetically distinct from P. adenostyloides. Therefore, it is recognized as an independent species, different from P. adenostyloides. The specific epithet "pentaphyllaria" was given based on its characteristic of having five phyllaries.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.57-57
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• 2022

A taxonomic revision of Deutzia in Korea was conducted based on critical observations of wild populations and herbarium specimens. Species delimitations were re-evaluated on the basis of morphological characters and distributions resulting in the recognition of five taxa. The texture of leaf, flower disk and capsules, the shape of leaf, inflorescence and capsules are useful diagnostic traits at the specific level. The five taxa are well distinguished from each other. Deutzia grandiflora var. baroniana can be distinguished by its distribution and calyx from Deutzia uniflora, which are morphologically similar. Deutzia paniculata Nakai, a rare and endemic plant, is characterized by having panicle in inflorescences. On the other hand, Deutzia glabrata Kom. is occasionally confused with Deutzia parviflora, but the most distinctive characters include the surface of leaves, the surface and size of capsules. Photographs and a key to five species of Deutzia were provided in addition to distribution.

• Ecology and Resilient Infrastructure
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• v.5 no.3
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• pp.180-188
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• 2018

Biopolymer based on microbial ${\beta}$-glucan and xanthan gum is a rising geotechnical material that can enhance the cohesion between soil particles and consequently reduce soil erosion. Recently, biopolymer is proposed to utilize for the riverbank strengthening. As an effort of the ecological assessment of biopolymer application in civil engineering, this study examined the effects of biopolymer on seed germination and growth of nine plant species inhabiting in the Korean riverside. Responses of above-ground growth to the biopolymer differed among plant species. One species grew less but others maintained their growth when plants were grown in the soil with biopolymer. In contrast, root grew more vigorously and root/shoot ratio decreased in the biopolymer across testing plant species. These results indicate that biopolymer application on the river bank likely stimulate root growth of native plant species, which, in turn, possibly reinforces riverbank. Species specific responses of above-ground growth implies that ecological effects of biopolymer application would depend on the species composition of the ecosystem.

• The Plant Pathology Journal
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• v.29 no.1
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• pp.99-104
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• 2013

To detect five plant viruses (Beet black scorch virus, Beet necrotic yellow vein virus, Eggplant mottled dwarf virus, Pelargonium zonate spot virus, and Rice yellow mottle virus) for quarantine purposes, we designed 15 RT-PCR primer sets. Primer design was based on the nucleotide sequence of the coat protein gene, which is highly conserved within species. All but one primer set successfully amplified the targets, and gradient PCRs indicated that the optimal temperature for the 14 useful primer sets was $51.9^{\circ}C$. Some primer sets worked well regardless of annealing temperature while others required a very specific annealing temperature. A primer specificity test using plant total RNAs and cDNAs of other plant virus-infected samples demonstrated that the designed primer sets were highly specific and generated reproducible results. The newly developed RT-PCR primer sets would be useful for quarantine inspections aimed at preventing the entry of exotic plant viruses into Korea.

• Journal of Ecology and Environment
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• v.31 no.3
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• pp.213-223
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• 2008

The increasing area of abandoned rice fields could provide new opportunities for wetland restoration in Asia. However, it is unknown how quickly or completely abandoned rice fields will recover from agricultural disturbances. We assessed water quality and plant community succession in abandoned rice fields with different hydrology in a mountain valley to understand the effects of hydrological regime on recovery. Water level, soil redox potential, water quality, plant composition, and primary production were measured. The sites, coded as D6, N13, and N16, had been recovering for 6, 13, and 16 years by 2006. N13 and N16 have been recovering naturally whereas D6 has been drained with a nearby dike and was tilled in 2001. The typical hydroperiods of D6, N13, and N16 were no surface water, permanently flooded, and seasonally flooded, respectively. The major change in vegetation structure of both D6 and N13 was the replacement of herbaceous species by woody species. Drawdown accelerated this change because Salix koreensis grew better in damp conditions than in flooded conditions. Phragmites japonica reduced plot-level plant species richness. The removal efficiency of $NH_4-N$, $NO_3-N$, and $PO_4-P$ from water varied seasonally, ranging between -78.8 to 44.3%, 0 to 97.5%, and -26.0 to 44.4%, respectively. In summary, abandoned rice fields quickly became suitable habitat for native wetland plant species and improved regional water quality. Variation among our sites indicates that it is likely possible to manage abandoned rice fields, mostly through controlling hydrology, to achieve site-specific restoration goals.

• The Plant Pathology Journal
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• v.25 no.1
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• pp.13-20
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• 2009

Ninety isolates of Colletotrichum species from new persimmon tree twigs and 50 isolates from pepper plant fruits were isolated via single-spore isolation. Of the 140 isolates, 26 were examined for mycelial growth, carbendazim sensitivity, and ITS sequence. Four of the isolates from the persimmon trees, which were cultivated exclusively in an orchard, showed fast mycelial growth and sensitivity to carbendazim, while five of the pepper isolates showed slower mycelial growth and were resistant to the fungicide. However, 17 isolates from persimmon trees cultivated with pepper plants in the same orchard showed slow mycelial growth like the pepper isolates and they were sensitive to carbendazim like the persimmon isolates. ITS sequence analysis of these 27 isolates led to the identification of the 22 persimmon isolates as C. gloeosporioides and the five pepper isolates as C. acutatum. PCR with species-specific primers confirmed that the 90 isolates from persimmon were C. gloeosporioides whereas the 50 isolates from pepper were C. acutatum. The 90 persimmon isolates of C. gloeosporioides and 50 pepper isolates of C. acutatum were compared by a wound inoculation test to determine their capacity for host cross-infection. All of the C. acutatum isolates from pepper caused typical symptoms of anthracnose on the fruits of pepper plants and twigs of persimmon; they differed from the C. gloeosporioides isolates from persimmon, more than 90% of which were able to infect only persimmon. Amplified fragment length polymorphism analysis revealed the existence of two groups (C. gloeosporioides and C. acutatum isolates group). At 80% genetic similarity, the C. gloeosporioides group was defined within four clusters, while the C. acutatum group was within three clusters. However, these clusterings were unrelated with the virulence of Colletotrichum species against pepper fruits.

• Korean Journal of Plant Taxonomy
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• v.44 no.2
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• pp.136-163
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• 2014

This study was carried out to investigate flora of Golji stream watershed in Gangwon province. The vascular plants which were collected in 11 times (from May 2012 to Nov. 2013) were identified as 825 taxa in total, including 124 families, 420 genera, 730 species, 8 subspecies, 78 varieties, 6 forms and 3 hybrids. In the flora of this area, Korean endemic species were 34 taxa, red list of vascular plants according to IUCN valuation basis were 34 taxa and 165 taxa of specially designated floristic regional indicator plants by the Ministry of Environment. Among those species investigated in this survey, 101 taxa were identified as specific species bio-geographically as compared to flora of other terrestrial regions; 37 of calcicolous plants, 64 with distribution in the northern part of the Korean peninsula. 34 aquatic taxa and 69 naturalized plants were recorded in the investigated area.

• Journal of Microbiology and Biotechnology
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• v.18 no.9
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• pp.1492-1495
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• 2008

Successful control of Xanthomonas oryzae pv. oryzicola, the causal agent of bacterial leaf streak, requires a specific and reliable diagnostic tool. A pathovar-specific PCR assay was developed for the rapid and accurate detection ofthe plant pathogenic bacterium Xanthomonas oryzae pv. oryzicola in diseased plant. Based on differences in a membrane fusion protein gene of Xanthomonas oryzae pv. oryzicola and other microorganisms, which was generated from NCBI (http://www.ncbi.nlm.nih.gov/) and CMR (http://cmr.tigr.org/) BLAST searches, one pair of pathovar-specific primers, XOCMF/XOCMR, was synthesized. Primers XOCMF and XOCMR from a membrane fusion protein gene were used to amplity a 488-bp DNA fragment. The PCR product was only produced from 4 isolates of Xanthomonas oryzae pv. oryzicola among 37 isolates of other pathovars and species of Xanthomonas, Pectobacterium, Pseudomonas, Burkholderia, Escherichia coli, and Fusarium oxysporum f.sp. dianthi. The results suggested that the assay detected the pathogen more rapidly and accurately than standard isolation methods.