Browse > Article
http://dx.doi.org/10.5423/PPJ.2009.25.1.013

Potential of Cross-infection of Colletotrichum Species Causing Anthracnose in Persimmon and Pepper  

Kim, Hye-Ryoung (Department of Plant Medicine, Chungbuk National University)
Lim, Tae-Heon (Research Institute of Agri-Bio Science, Samhoub, Co., Ltd.)
Kim, Joo-Hyung (Department of Plant Medicine, Chungbuk National University)
Kim, Young-Ho (Department of Applied Biology and Chemistry, Seoul National University)
Kim, Heung-Tae (Department of Plant Medicine, Chungbuk National University)
Publication Information
The Plant Pathology Journal / v.25, no.1, 2009 , pp. 13-20 More about this Journal
Abstract
Ninety isolates of Colletotrichum species from new persimmon tree twigs and 50 isolates from pepper plant fruits were isolated via single-spore isolation. Of the 140 isolates, 26 were examined for mycelial growth, carbendazim sensitivity, and ITS sequence. Four of the isolates from the persimmon trees, which were cultivated exclusively in an orchard, showed fast mycelial growth and sensitivity to carbendazim, while five of the pepper isolates showed slower mycelial growth and were resistant to the fungicide. However, 17 isolates from persimmon trees cultivated with pepper plants in the same orchard showed slow mycelial growth like the pepper isolates and they were sensitive to carbendazim like the persimmon isolates. ITS sequence analysis of these 27 isolates led to the identification of the 22 persimmon isolates as C. gloeosporioides and the five pepper isolates as C. acutatum. PCR with species-specific primers confirmed that the 90 isolates from persimmon were C. gloeosporioides whereas the 50 isolates from pepper were C. acutatum. The 90 persimmon isolates of C. gloeosporioides and 50 pepper isolates of C. acutatum were compared by a wound inoculation test to determine their capacity for host cross-infection. All of the C. acutatum isolates from pepper caused typical symptoms of anthracnose on the fruits of pepper plants and twigs of persimmon; they differed from the C. gloeosporioides isolates from persimmon, more than 90% of which were able to infect only persimmon. Amplified fragment length polymorphism analysis revealed the existence of two groups (C. gloeosporioides and C. acutatum isolates group). At 80% genetic similarity, the C. gloeosporioides group was defined within four clusters, while the C. acutatum group was within three clusters. However, these clusterings were unrelated with the virulence of Colletotrichum species against pepper fruits.
Keywords
Colletotrichum acutatum; C. gloeosporioides; cross-infection; pepper; persimmon;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 3  (Related Records In Web of Science)
연도 인용수 순위
1 Fagan, H. J. 1979. Postbloom fruit drop, a new disease of citrus associated with a form of Colletotrichum gloeosporioides. Ann. Appl. Bioi. 91:13-20   DOI
2 Freeman, S. and Shabi, E. 1996. Cross-infection of subtropical and temperate fruits by Colletotrichum species from various hosts. Physiol. Mol. Plant Pathol. 49:395-404   DOI   ScienceOn
3 Freeman, S., Shabi, E. and Katan, T. 2000. Characterization of Colletotrichum acutatum causing anthracnose of anemone (Anemone coronaria L.). Appl. Environ. MlCrobiol. 66:5267-5272   DOI   ScienceOn
4 Kim, J., Park, S. Choi, W., Lee, Y. and Kim, H. T. 2008. Characterization of Colletotrichum isolates causing anthracnose or pepper in Korea. Plant Pathol. J 24: 17-23   DOI   ScienceOn
5 Lee, D. W., Lee, G C., Lee, S. W., Park, C-G, Choo, H. Y. and Shin, C-H. 2001. Survey on pest management practice and scheme of increasing income in sweet persimmon farms in Korea (in Korean). Korean J Pest. Sci. 5:45-49
6 Manandhar, J. B., Hartman, G L. and Wang, T. C. 1995. Anthracnose development of pepper fruits inoculated with Colletotrichum gloeosporioides. Plant Dis. 79:380-383   DOI
7 Park, K. S. and Kim, C. H. 1992. Identification, distribution and etiological characteristics of anthracnose fungi of red pepper in Korea. Korean J Plant Pathol. 8:61-69
8 Peres, N. A., Kuramae, E. E., Dias, M. S. C. and de Souza, N. L. 2002. Identification of Colletotrichum species. Affecting fruit harvest in Brazil. J Phytopathol. 150: 128-134   DOI   ScienceOn
9 Quimio, T. H. and Quimio, A. J. 1975. Notes of Philippine grape and guava anthracnose. Plant Dis. Rept. 59:221-224
10 Sacristan, S. and Garcia-Arenal, F. 2008. The evolution of virulence and pathogenicity in plant pathogen popUlations. Mol. Plant Pathol. 9:369-384   DOI   ScienceOn
11 Than, P. P., Jeewon, R., Hyde, K. D., Pongsupasamit, S., Mongkolpom, O. and Taylor, P. W. J. 2008. Characterization and pathogenicity of Colletotrichum species associated with anthracnose on chilli (Capsicum spp.) in Thailand. Plant Pathol. 57:562-572   DOI   ScienceOn
12 Lim, T. H. and Choi, Y-H. 2006. Response of several fungicides of Colletotrichum gloeosporioides isolates obtained from persimmon in Sanju. Res. Plant Dis. 12:99-102   DOI   ScienceOn
13 Kang, B. K., Min, J. Y., Kim, Y. S., Park, S. W., Van Bach, N. and Kim, H. T. 2005. Semi-selective medium for monitoring Colletotrichum acutatum causing pepper anthracnose in the field. Res. Plant Dis. 11:21-27   DOI   ScienceOn
14 Freeman, S., Horowitz, S. and Sharon, A. 2001. Pathogenic and nonpathogenic lifestyles in Colletotrichum acutatum from strawberry and other plants. Phytopathology 91 :986-992   DOI   ScienceOn
15 Kim, Y-S., Min, J. Y., Kang, B. K., Van Bach, N., Choi, W. B., Park, E. W. and Kim, H. T. 2007. Analyses of the less benzimidazole- sensitivity of the isolates of Colletotrichum spp. causing anthracnose in pepper and strawberry. Plant Pathol. J 23: 187-192   DOI   ScienceOn
16 Peres, N. A., Timmer, L. W., Adaskaveg, L. E. and Correll, J. C. 2005. Lifestyles of Colletotrichum acutatum. Plant Dis. 89:784-796   DOI   ScienceOn
17 Agostini, J. P., Timmer, L. W. and Mitchell, D. J. 1992. Morpho logical and pathological characteristics of strains of Colletotrichum gloeosporioides from ci1rus. Phytopathology 82: 1377-1382   DOI
18 Freeman, S. and Katan, T. 1997. Identification of Colletotrichum species responsible for anthracnose and root necrosis of strawberry in Israel. Phytopathology 87:516-521   DOI   ScienceOn
19 Liyanage, H. D., McMillan, R. T. and Kistler, H. C. 1992. Two genetically distinct populations of Colletotrichum gloeosporiaides from citrus. Phytopathology 82:1371-1376   DOI
20 Alahakoon, P. W., Brown, A. E. and Sreenivasaprasad, S. 1994. Cross-infection potential of genetic groups of Colletotrichum gloeosporioides on tropical fruits. Physiol. Mol. Plant Pathol. 44:93-103   DOI   ScienceOn
21 Freeman, S., Katan, T. and Shabi, E. 1998. Characterization of Colletotrichum species responsible for anthracnose disease of various fruits. Plant Dis. 82:596-605   DOI   ScienceOn
22 Mills, P. R., Sreenivasaprasad, S. and Brown, A. E. 1992. Detection and differentiation of Collettorichum gloeosporioides isolates using PCR. FEMS Microbiol. Lett. 98:137-143   DOI   ScienceOn
23 Berstein, B., Zehr, E. J., Dean, R. A. and Shabi, E. 1995. Characteristics of Colletotrichum from peach, apple, pecan, and other hosts. Plant Dis. 79:478-482   DOI   ScienceOn
24 Darvas,J.M.,;Kotze, J.M.;Wehner, F.C. 1987. Pathogenicity of fungi causing pre- and postharvest diseases of avocado fruits. Phytophylactica 19:489-493
25 Vos, P., Hogers, R., Bleeker, M., Reijans, M., Vandelee, T., Homes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M., Zabeau, M., 1995. AFLP-A new technique for DNA-fingerprinting. Nucleic Acids Res. 23:4407-4414   DOI   ScienceOn
26 Mass, J. L. and Howard, C. M. 1985. Variation of several anthracnose fungi in virulence to strawberry and apple. Plant Dis. 69: 164-166   DOI
27 Gunnell, P. S. and Gubler, W. D. 1992. Taxonomy and morphology of Colletotrichum species pathogenic to strawberry. Mycologia 84:157-165   DOI
28 Denoyes, B. and Baudry, A. 1995. Species identification and pathogenicity study of French Colletotrichum strains isolated from strawberry using morphological and cultural characteristics. Phytopathology 85:53-57   DOI   ScienceOn
29 Brown, A. E. and Soepena, H. 1994. Pathogenicity of Colletotrichum acutatum and Colletotrichum gloeosporioides on leaves of Hevea spp. Mycol. Res. 98:264-266   DOI   ScienceOn
30 Freeman, S., Katan, T. and Shabi, E. 1996. Characterization of Colletotrichum gloeosporioides isolates from avocado and almond fruits with molecular and pathogenicity tests. Appl. Environ. Microbial. 62:1014-1020   DOI
31 Timmer, L. W., Agostini, J. P., Zitko, S. E. and Zulfiquar, M. 1994. Postbloom fruit drop, an increasingly prevalent disease of citrus in the Americas. Plant Dis. 78:329-334   DOI   ScienceOn
32 Kwon, J-H., Kang, S-W. and Park, C-S. 2000. Cultural characteristics of Colletotrichum gloeosporioides causing anthracnose of persimmon. Plant Dis. Res. 6:48-50 (in Korean)