• Title/Summary/Keyword: Specific oxygen uptake rate

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Performance of a Novel Bioreactor Equipped with Moving Membrane Tube-Aeration System (회전하는 산소전달장치가 부착된 동물세포 배양기의 조업 성능에 관한 고찰)

  • Kim, Young-Nam;Jeon, Byung-Cheol;Lee, Jin-Ha;Lee, Hyeon-Yong
    • Microbiology and Biotechnology Letters
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    • v.21 no.4
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    • pp.348-353
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    • 1993
  • The optimal conditions for operating a moving-aeration bioreactor were determined as 30rpm and 150 (ml/min) of air flow rate, which can yield ca. 7.3 (l/h)of maximum mass transfer coefficient. It was also found that the agitation speed played much much important role than air input rate in oxgen transfer into the medium. $2.6{\times}10^6$ (cells/ml) and 0.6 (ml/l) of maximum cell denisty and IL-2 production were observed in batch cultivation of IL-2 producing BHK cell line. 0.53 (mM/l/h) of oxygen uptake rate was also estimated. The performance of a moving-aeration bioreactor (specific growth rate and oxygen uptake rate, etc.) was superior to other culture systems, such as cell-life and static membrane aeration bioreactors. Ii must be useful to apply this reactor to many culture processes by improving structural limitations in scaling-up the system.

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Measurement of Viable Cell Number in Mixed Culture Based on Microbial Respiration Rate (미생물 호흡속도에 기초한 혼합배양중의 생균수 측정)

  • Veljkoic, V.B;;C.R.Engler
    • Microbiology and Biotechnology Letters
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    • v.20 no.6
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    • pp.687-692
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    • 1992
  • A simple method to determine viable cell numbers of each species in mixed culture was developed. The oxygen uptake rate (OUR) equals to the product of the specific OUR and the size of the microbial population. In a mixed culture, the OUR is a result of the respiration activities of each sub-population. The OUR was determined from the slope of the linear relationship between time and the decrease of dissolved oxygen concentration when aeration was stopped. The specific OUR was calculated from the slope of the viable cell number versus OUR curve. These values for C. lusitaniae at 20 and $30^{\circ}C$ were $1.36{\times}10^{-9}$ and $3.90{\times}10^{-9}$ and those for P tannoPhilus at 20 and $30^{\circ}C$ were $0.59{\times}10^{-9}$ and $1.86{\times}10^{-9}$ [(%/s)/(cells/ml)J. respectively. Using these values, viable cell numbers were calculated after the OURs of mixed culture at two temperatures were measured. A good agreement between the viable cell numbers determined by this method and by plate count was obtained.

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Saccharomyces cerevisiae에서 myo-Inositol 결핍에 의한 Respiratory capacity의 감소

  • 정경환;이준식
    • Microbiology and Biotechnology Letters
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    • v.24 no.4
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    • pp.485-492
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    • 1996
  • myo-Inositol, a growth factor for Saccharomyces cerevisiae (S. cerevisiae), has been known to be incorporated into phosphatidylinositol (PI), which is a kind of phospholipid in the cell membrane, by a membrane-associated PI-synthesizing enzyme. The deficiency of myo-inositol in S. cerevisiae adversely affected the membrane structure and function. On the basis of biochemical functions of myo-inositol, the effect of deficiency of myo-inositol on the aerobic glucose metabolism was investigated by measuring specific oxygen uptake rate (Q$_{O2}$) used as an indicator representing the respiratory capacity of S. cerevisiae in batch and continuous cultures. The respiratory capacity of aerobic glucose metabolism in S. cerevisiae was also monitored after glucose pulse-addition in a continuous culture (D=0.2, 1/hr), in which glucose was utilized through respiratory metabolism. The deficiency of myo-inositol was found to lead to both the decrease of the maximum specific oxygen uptake rate (Q$_{O2max}$) observed from the batch as well as in the continuous culture experiment and the decrease of the respiratory capacity of aerobic glucose metabolism of S. cerevisiae determined from the glucose pulse-addition experiment, in which the glucose flux into respiratory and fermen- tative metabolism was quantitatively analyzed.

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Evaluation of SBBR Process Performance Focused on Nitrogen Removal with External Carbon Addition (외부탄소원을 사용한 SBBR의 공정 특성 및 질소제거)

  • Han, Hyejeong;Yun, Zuwhan
    • Journal of Korean Society on Water Environment
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    • v.22 no.3
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    • pp.566-571
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    • 2006
  • A sequencing batch biofilm reactor (SBBR) operated with a cycle of anaerobic - aerobic - anoxic - aerobic has been evaluated for the nutrient removal characteristics. The sponge-like moving media was filled to about 10% of reactor volume. The sewage was the major substrate while external synthetic carbon substrate was added to the anoxic stage to enhance the nitrogen removal. The operational results indicated that maximum T-N and T-P removal efficiencies were 97% and 94%, respectively were achieved, while COD removal of 92%. The observations of significant nitrogen removal in the first aerobic stage indicated that nitrogen removal behaviour in this SBBR was different to conventional SBR. Although the reasons for aerobic nitrogen removal has speculated to either simultaneous nitrification and denitrification or anoxic denitrification inside of the media, further researches are required to confirm the observation. The specific oxygen uptake rate (SOUR) test with biofilm and suspended growth sludge indicated that biofilm in SBBR played a major role to remove substrates.

Assessment of Characteristics and Field Applicability with TPA By-Product as Alternative External Carbon Source (대체 외부탄소원으로서의 TPA 생산부산물 특성 및 현장적용성 평가)

  • Jung, In-Chul;Jun, Sung-Gyu;Sung, Nak-Chang
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.5
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    • pp.480-486
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    • 2006
  • On account of exchanging main process from chemical precipitation for MLE(Modified Ludzark-Ettinger), an external carbon source was required for supplementation of carbon source shortage that was needed biological denitrification in the S sewage treatment plant(S-STP). In this study, NUR(nitrate uptake rate), OUR(oxygen uptake rate) test and a field application test was conducted for the applicability assessment of Terephtalic acid(TPA) by-product contained about 4.7% acetate as alternative external carbon source. As the results, TPA by-product shows more rapid acclimation than methanol, 8.24 mg ${NO_3}^--N/g$ VSS/hr specific denitrification rate, 3.70 g $COD_{Cr}/g\;NO_3$ C/N ratio and 99.4% readily biodegradable COD contents. In the results of field application, the nutrient removal efficiency was high and effluent T-N concentration is 8.2 mg/L. It is concluded that TPA by-product is the proper alternative external carbon source.

Abnormal Behavior of Ordinary Heterotrophic Organism Active Biomass at Different Substrate/Microorganisms Ratios in Batch Test (회분식 실험 Substrate/Microorganisms 비에 따른 종속영양미생물의 특이거동 연구)

  • Lee, Byung-Joon;Wentzel, M.C.;Ekama, G.A.;Min, Kyung-Sok
    • Journal of Korean Society on Water Environment
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    • v.20 no.3
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    • pp.197-205
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    • 2004
  • Batch test methods have developed for a long time to measure kinetic and stoichiometric parameters which are required to perform steady state design and mathematical modelling of activated sludge processes. However, at various So/Xo ratios, abnormal behaviors of ordinary heterotrophic organism in batch tests have been reported in many researches. Thus, in this research, abnormal behaviors of heterotrophs in batch tests were investigated at various So/Xo conditions by measuring and interpreting oxygen utilization rate. As So/Xo ratio increased, the calculated values of maximum specific growth rates, ${\mu}_{H,max}$ and $K_{MP,max}$, increased. However, at a certain point of So/Xo (around 10mgCOD/mgMLAVSS), ${\mu}_{H,max}$ and $K_{MP,max}$ values started to decrease. According to this observation, three prominent behaviours of heterotrophs were identified at various So/Xo conditions. (1) At low So/Xo region (below 5 mgCOD/mgMLAVSS), the oxygen utilization rate of heterotrophs in batch tests were almost stable and consequently yielded lower maximum specific growth rate. (2) At high So/Xo region (up to 5~10 mgCOD/mgMLAVSS), oxygen utilization rate incresed sharply with time and indicated more upward curvature than the predicted OUR with conventional activated sludge model, which consists of single hetetrotrophs group. Thus, in this region, competition model of two organisms, fast-grower and slow-grower, seemed to be appropriate. (3) At extremely high So/Xo region (over 10mgCOD/mgMLAVSS), significant oxygen utilization rate was still observed even after depletion of readily biodegradable COD. This might be caused by retarded utilization of intermediates which were generated by self inhibition mechanism in the process of RBCOD uptake.

Estimation of Kinetic Parameters for Biomass Growth Using Micro-nano Bubbles Reactor (마이크로-나노버블 반응조를 이용한 미생물성장 동력학 계수의 추정에 관한 연구)

  • Han, Young-Rip;Jung, Byung-Gil;Jung, Yoo-Jin;Cho, Do-Hyun;Sung, Nak-Chang
    • Journal of Environmental Science International
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    • v.19 no.5
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    • pp.647-653
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    • 2010
  • The objectives of this research are to evaluate and compare the oxygen transfer coefficients($K_{La}$) in both a general bubbles reactor and a micro-nano bubbles reactor for effective operation in sewage treatment plants, and to understand the effect on microbial kinetic parameters of biomass growth for optimal biological treatment in sewage treatment plants when the micro-nano bubbles reactor is applied. Oxygen transfer coefficients($K_{La}$) of tap water and effluent of primary clarifier were determined. The oxygen transfer coefficients of the tap water for the general bubbles reactor and micro-nano bubbles reactor were found to be 0.28 $hr^{-1}$ and 2.50 $hr^{-1}$, respectively. The oxygen transfer coefficients of the effluent of the primary clarifier for the general bubbles reactor and micro-nano bubbles reactor were found be to 0.15 $hr^{-1}$ and 0.91 $hr^{-1}$, respectively. In order to figure out kinetic parameters of biomass growth for the general bubbles reactor and micro-nano bubbles reactor, oxygen uptake rates(OURs) in the saturated effluent of the primary clarifier were measured with the general bubbles reactor and micro-nano bubbles reactor. The OURs of in the saturated effluent of the primary clarifier with the general bubbles reactor and micro-nano bubbles reactor were 0.0294 mg $O_2/L{\cdot}hr$ and 0.0465 mg $O_2/L{\cdot}hr$, respectively. The higher micro-nano bubbles reactor's oxygen transfer coefficient increases the OURs. In addition, the maximum readily biodegradable substrate utilization rates($K_{ms}$) for the general bubbles reactor and micro-nano bubbles reactor were 3.41 mg COD utilized/mg active VSS day and 7.07 mg COD utilized/mg active VSS day, respectively. The maximum specific biomass growth rates for heterotrophic biomass(${\mu}_{max}$) were calculated by both values of yield for heterotrophic biomass($Y_H$) and the maximum readily biodegradable substrate utilization rates($K_{ms}$). The values of ${\mu}_{max}$ for the general bubbles reactor and micro-nano bubbles reactor were 1.62 $day^{-1}$ and 3.36 $day^{-1}$, respectively. The reported results show that the micro-nano bubbles reactor increased air-liquid contact area. This method could remove dissolved organic matters and nutrients efficiently and effectively.

The Estimation of Bio-kinetic Parameters using Respirometric Analysis (산소이용률을 이용한 생물학적 동력학 계수 추정)

  • Choung, Youn-Kyoo;Kim, Han-Soo;Yoo, Sung-In
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.1
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    • pp.11-19
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    • 2000
  • In order to predict the performance of biological wastewater treatment plant, the kinetic parameters and stoichiometric coefficient must be known. The theories and experimental procedures for determining the biological kinetic parameters were discussed in this study. Respirometric analysis in the batch reactor was carried out for the experimental assessment of kinetic parameters. A simple procedure to estimate kinetic parameters of heterotrophs and autotrophs under aerobic condition was presented. The difficulties in the interpretation of COD and VSS measurements encouraged the conversion of respirometric data to growth data. Maximum specific growth rate, yield coefficient, half saturation constant and decay rate of heterotrophic biomass were obtained from OUR(Oxygen Uptake Rate) data. Maximum specific growth rate of autotrophic biomass was obtained from the increase of nitrate concentration. The aim of this paper is to estimate the kinetic parameters of heterotrophic and autotrophic biomass by means of the respirometric analysis of activated sludge behavior in the batch reactors. These procedures may be used for the activated sludge modeling with complex kinetic parameters.

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Determination of Biological kinetic Parameters for Pharmaceutical Wastewater (제약 폐수의 생물학적 동력학 계수 측정)

  • Lee Young-Rak;Choi Kwang-Keun;Lee Jin-Won
    • KSBB Journal
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    • v.21 no.1 s.96
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    • pp.49-53
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    • 2006
  • The aim of this research is to estimate the values of biological kinetic parameters of pharmaceutical wastewater for understanding biochemical properties. Maximum specific growth rate (${\mu}m$), yield coefficient (Y), and half-velocity coefficient (KS) were determined using oxygen uptake rate (OUR), and the results were 10.49/day (0.437/hr), 0.655, and 38.89 mg/L, respectively. Measured ${\mu}max$ by nonlinear regression of Monod equation was 10.63/day (or 0.443/hr), and this value was similar with above result. These parameters may be used to increase efficiency of pharmaceutical wastewater treatment and to determine amount of oxygen needed to removal BOD and dissolved oxygen in activated sludge process.

Kinetics of Cultivating Large Quantities of Mammalian Cells (tPA생산을 위한 동물 세포 배양에 관한 동력학적 연구)

  • 이현용
    • Microbiology and Biotechnology Letters
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    • v.16 no.4
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    • pp.282-286
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    • 1988
  • Growth kinetic parameters for mass cultivation of Chinese Hamster Ovary (CHO) cells are estimated by measuring oxygen uptake rates. It Is found that there is strong correlation between cell growth and oxygen consumption, showing that correlation factor is 0.83. Derived linear model predicts actual cell density very well. It tells that oxygen uptake rate can play important role in indirectly measuring cell density when conventional method of estimating cell density is no longer meaningful due to heavy cell clumpings. Cell yield per oxygen consumption, $Y_{\chi}o$ and mass transfer coefficient for oxygen, Ka are also estimated as 1.26$\times$10$^4$cells/mmole $O_2$ consumed and 1.01/h, respectively. Average specific growth rate over all runs is 2.891/day for CHO cells with producting 2 grams of tPA per day under continuous perfusion operations.

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