• 환경생물
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• 제31권3호
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• pp.225-231
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• 2013

동해안의 암반조간대 무척추동물상의 분포 특성을 연구하기 위하여 독도를 비롯한 울릉도, 경상북도의 경주, 포항, 영덕, 울진 그리고 강원도 등 총 19개 정점의 암반 조간대무척추동물상을 조사하여 출현종의 유사도를 공통종의 출현비율 (%)과 Bray-Curtis similarity matrix를 이용한 집괴분석과 MDS를 이용하여 분석하였으며 SIMPER를 이용하여 독도와 그 외의 동해안 암반저서 무척추동물의 특징종을 선출하였다. 공통종의 출현비와 집괴분석결과, 가장 가까이 위치한 울릉도를 제외하면, 경상북도의 영덕이 가장 높은 유사도를 보였으나, 전체적으로 독도 암반저서생태계는 다른 동해안 연안과 차이를 보인 것으로 나타났다.

• 대한의생명과학회지
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• 제13권4호
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• pp.293-304
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• 2007

The emergence of extended spectrum $\beta$-lactamase (ESBL) producing bacteria is worldwide concern. Until recently, the most frequently identified strains in the Republic of Korea were E. coli and Klebsiella spp. The incidence of resistance to extended spectrum $\beta$-lactam antibiotics is increasing in Wonju city, Korea. Total 57 strains of ESBL producing E. coli and Klebsiella species were isolated from Wonju Christian Hospital during a 9 month-period from April to December, 2003. To determine the prevalence and genotypes of the ESBL producing clinical isolates, antibiotic susceptibility and ESBL activity test by VITEK system and double disk synergy (DDS) test, and PCR based genotyping were performed. Fourteen (82%) isolates of 17 ESBL producing E. coli were found to have $bla_{TEM}$ gene and 5 (29%) isolates were found to have $bla_{CTX-M}$ gene by polymerase chain reaction (PCR). Thirty (75%) isolates of 40 ESBL producing Klebsiella species with $bla_{TEM}$ gene, 38 (95%) isolates with $bla_{SHV}$ gene, and 7 (20%) isolates with $bla_{CTX-M}$ type gene were also identified. Enterobacterial repetitive intergenic consensus (ERIC) PCR and similarity index by dendrogram for genetical similarity to band pattern of each clinical isolates were examined. ESBL producing E. coli were grouped into 6 clusters up to 84% of similarity index and Klebsiella species were grouped into 12 clusters up to 76% of similarity index. In conclusion, ESBL producing clinical isolates were characterized with the results from antimicrobial resistance pattern and genetical similarity using ERiC PCR.

• 한국환경복원기술학회지
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• 제20권2호
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• pp.13-35
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• 2017

This study was carried out to analyze vegetation structure and change of the disturbed area in forest ecosystem(FE), riparian ecosystem(RE) and coastal ecosystem(CE) related to Regional Ecological Network at Chungnam province through constancy and dominance analysis, species diversity index, similarity index and canonical correlation analysis. Data were collected from April to October in 2015. As a result of constancy and dominance analysis of forest disturbed area, non-effective species(NES) was 30 species(17.0%), exported species(ES) was 98 species(55.7%) and imported species(IS) was 48 species(27.3%) among the total 176 species, respectively. In riparian disturbed area with total 139 species, there were 16 NES(11.5%), 98 ES(70.5%) and 25 IS(18.0%) respectively. In coastal disturbed area with 140 species, there were 20 NES(14.3%), 88 ES(62.9%) and 32 IS(22.9%) respectively. In all types of disturbed areas, the ratio of ES was higher than IS. As a result of species diversity, species richness and shannon's diversity index of disturbed area decreased in all kinds of crown strata such as tree, subtree, shrub and herb layer compared to the control area. As a result of similarity index, that of each type between control site and disturbed site was 0.374 in FE, 0.329 in CE and 0.259 in RE in the order. As a result of the CCA analysis, the number of present species, vine plants ratio and exported species ratio in disturbed area of FE and RE were decreased, and the naturalized plant ratio, imported species ratio and herb ratio were increased. But environmental factors of CE were not shown any clear tendency. In conclusion, many species occupied in control site disappeared into the disturbed area, and the naturalized plants and herb species were abundantly imported in the area. Therefore, it was considered that this study could be applied to the development of long-term and short-term ecological restoration techniques in view of vegetation changes.

• 한국발생생물학회지:발생과생식
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• 제20권4호
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• pp.379-385
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• 2016

Seven oligonucleotides primers were shown to generate the shared loci, specific loci, unique shared loci to each species and shared loci by the three species which could be obviously scored. In the present study, 7 oligonucleotides primers produced 401 total loci in the Styela clava (SC) species, 390 in the Halocynthia roretzi (HR) and 434 in the Styela plicata (SP), respectively. Seven oligonucleotides primers generated 275 specific loci in the SC, 341 in the HR and 364 in the SP species, respectively. The oligonucleotides primer BION-23 generated 28 unique loci to each species in the SP species. Especially, the oligonucleotides primer BION-25 produced 7 unique loci to each species, which were identifying each species in the SP species. BION-17 distinguished 21 shared loci by the three ascidian species, major and/or minor fragments of sizes, which were identical in almost all of the samples. Based on the average bandsharing values of all samples, the similarity matrix ranged from 0.519 to 0.774 in the SC species, from 0.261 to 0.683 in the HR species and from 0.346 to 0.730 in the SP species. As regards average bandsharing value (BS) results, individuals from SC species ($0.661{\pm}0.081$) exhibited higher bandsharing values than did individuals from HR species ($0.555{\pm}0.074$) (P<0.05). The dendrogram obtained by the seven oligonucleotides primers indicates three genetic groups. In three ascidian species, the shortest genetic distance (0.071) exhibiting significant molecular difference was also between individual no. 20 and no. 21 within the SP species.

• Ecology and Resilient Infrastructure
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• 제5권3호
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• pp.145-155
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• 2018

In a monsoonal climate, the soil seed bank can play an important role in plant regeneration after the severe annual floods that disturb above-ground vegetation within the riparian zone. To investigate the relationship between the soil seed bank and vegetation, we measured the species composition of the soil seed bank and the extant above-ground vegetation in six major plant communities (Artemisia selengensis, Miscanthus sacchariflorus, Persicaria nodosa, Phalaris arundinacea, Phragmites japonica, and Rorippa palustris) in the Cheongmicheon Stream, Korea. A total of 21 species germinated from the floodplain soil seed banks. The most diverse seed bank (21 species) was found in the A. selengensis community, wheres the lowest number of species was found in the R. palustris community (2 species). Most soil seed banks were composed of annuals (90%), exceptions being Rumex crispus and Artemisia princeps, which are perennial ruderals. The similarity of species composition between the soil seed bank and above-ground vegetation was low with Sorensen's similarity indices averaging 29% (range 12 - 42%). Crucially, existing dominant perennials of the extant vegetation including A. selengensis, M. sacchariflorus, P. japonica and P. arundinacea were absent from the soil seed bank. In conclusion, the soil seed banks of the floodplains of the Cheongmicheon Stream were mainly composed of viable seeds of ruderal plants, which could germinate rapidly after severe flood disturbance. The soil seed bank may, therefore, be useful for the restoration of the early succession stages of riparian vegetation after flood disturbances.

• 원예과학기술지
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• 제19권2호
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• pp.159-162
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• 2001

한국에 자생하는 22지역의 바위솔속 식물들의 유전적 근연관계를 구명하기 위하여 RAPD분석을 실시하였다. 18종의 바위솔속 식물은 3개의 군(A, B, C 군)으로 집괴되었으며, 나머지 4종은 어떤 분류군에도 속하지 않았다. A군인 연화바위솔(No.18-No.22)은 유사도가 66.3-73.9%로 낮게 나타났으며, B군인 둥근바위솔(No.12-No.17)도 유사도 지수가 69.7-83.7%로 비교적 낮게 나타났다. C군인 바위솔(No.3-No.9)은 안면도 수집종(No.9)을 제외한 유사도 지수가 84.2-92.3%로 높아, 지역종 간에 변이가 적은 비교적 유전적으로 안정된 종으로 생각되었다.

• Journal of Crop Science and Biotechnology
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• 제10권1호
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• pp.27-32
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• 2007

RAPD, Inter-SSR, and AFLP markers were used to assess the genetic diversity of lettuce cultivars and the phylogenetic relationships in Lactuca spp. A total of 216 polymorphic bands from seven RAPD primers, four Inter-SSR primers, and five AFLP primer combinations were used to elucidate the genetic similarity among lettuce cultivars. Forty-four lettuce accessions were subdivided into discrete branches according to plant type: crisphead, butterhead, and stem type, with some exceptions. The leafy- and cos-type accessions were intermingled in other groups with no discrete branch indicating that these are more diverse than others. Three accessions, including the Korean cultivar 'Cheongchima', the Korean local landrace 'Jinjam', and the German cultivar 'Lolla Rossa' were classified as the most diverse accessions. Twenty bands were unique in specific cultivars. Among these, three were specific in a plant type; one in Korean leafy type, one in crisphead type, and one in cos type lettuce. In the phylogenetic analysis among Lactuca species, L. saligna, L. serriola, and L. georgica clustered in a sister branch of the L. sativa complex. Two L. virosa accessions show the highest intra-specific relationships. L. perennis outlied from all the other Lactuca species at a genetic similarity of 0.53 and clustered with two Cichorium species, C. intybus and C. endivia, with genetic similarity of 0.67. The phylogenetic tree was supported by data from polymorphism of chloroplast genome which was revealed by PCR-RFLP.

• 한국자원식물학회지
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• 제25권3호
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• pp.329-338
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• 2012

Genus Taraxacum has been widely used as a folkloric medicine for treatment of diverse diseases. The genetic diversity and relationship among 32 accessions belonging to five Taraxacum species (T. mongolicum T. coreanum, T. coreanum var. flavescens, T. officinale and T. laevigatum) which collected from field, mountain, island and seaside of Korea were evaluated using ISSR markers. A total of 142 ISSR loci detected in the overall species were all polymorphic loci (100%) and interspecies polymorphisms obtained from Korean native and naturalized species were 98.2% and 94.5%, respectively. The genetic similarity matrix (GSM) among 32 accessions ranged from 0.025 to 0.860 with an average of 0.303. According to the clustering analysis, the Korean native species and naturalized species were divided two major clusters. In addition, the different species were divided into independent groups except for the T. coreanum and T. coreanum var. flavescens, and all the 32 accessions could be classified into 7 categories. The study findings indicate that Taraxacum accessions have a high genetic diversity and the dandelion accessions as breeding materials can be effectively utilized for the improvement of Taraxacum breeding.

• 한국수산과학회지
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• 제47권6호
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• pp.945-959
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• 2014

This study was conducted to provide basic data for optimal fisheries management through the accurate understanding the catches, fishing level, species composition, distribution characteristics and fisheries status of trammel net in Gangwon Province. As the results of trammel net survey, Pisces were collected 77 species, 1,983.0 kg, Crustacea 9 species, 569.0 kg, Cephalopoda 5 species, 75.3 kg, Gastropoda 12 species, 16.5 kg in the coastal waters of Sokcho and Pisces 55 species, 3,681.5 kg, Crustacea 6 species, 2,229.8 kg, Gastropoda 10 species, 72.8 kg, Cephalopoda 4 species, 10.1 kg in the coastal waters of Donghae, respectively. The catches of important species with season and depths showed that the main target species is Pleuronectidae spp. at all depth and Aptocyclus ventricosus at <100 m in Sokcho, Liparis spp. and Dasycottus setiger at 200-500 m in Donghae, respectively. The mean total length (cm) of Gadus macrocephalus and Aptocyclus ventricosus didn't appear significant difference, but the mean total length of Pleuronectidae spp., Liparis spp. and D. setiger in Donghae is bigger than in Sokcho. From the cluster and MDS analysis based on Bray-Curtis similarity matrix of fourth root transformed, catches data of dominant species in the coastal waters of Sokcho and Donghae was divided into three different groups of the demersal organisms community in 12 survey of Donghae (Group A) and Jul., Oct.-Dec. survey of Sokcho (Group B) and Jan.-Jun. and Aug.-Sep. survey of Sokcho (Group C).

• 한국가구학회지
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• 제20권1호
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• pp.95-104
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• 2009

The study was carried out to analyze wood quality of coffin board, the wood species of coffin excavated in Youngdong, Korea. All coffin woods were identified as hard pines, Korean red pine(Pinus densiflora S. & Z.). According to wood quality of coffins, inner coffins of Bae Cheon-Jo and his wife showed similarity and dissimilarity comparing with several literatures. The similarity was in the structure of panels, bottom lining board and charcoal layer, and the dissimilarity was in jointing. From the analysis of fibrous materials attached to coffin woods, the paper mulberry and cotton fibers were identified. In the inner coffin of Bae Cheon-Jo, tool traces by handle planer were observed.