• ALGAE
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• v.33 no.1
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• pp.1-19
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• 2018

Members of the family Parviluciferaceae (Alveolata, Perkinsozoa) are the well-known dinoflagellate parasitoids along with Amoebophrya ceratii species complex and parasitic chytrid Dinomyces arenysensis and contain six species across three genera (i.e., Parvilucifera infectans, P. sinerae, P. rostrata, and P. corolla, Dinovorax pyriformis, and Snorkelia prorocentri) so far. Among Parvilucifera species, the two species, P. infectans and P. sinerae, are very similar or almost identical each other morphologically and genetically, thereby make it difficult to distinguish between the two. The only main difference between the two species known so far is the number of sporangium wall (i.e., 2 layers in P. infectans vs. 3 layers in P. sinerae). During sampling in Masan bay, Korea during the spring season of 2015, the dinoflagellate Akashiwo sanguinea cells infected by the parasite Parvilucifera were observed and this host-parasite system was established in culture. Using this culture, its morphological and ultrastructural features with special emphasis on the variation in the number of sporangium wall over developmental times, were investigated. In addition, the sequences of rDNA regions and ${\beta}-tubulin$ genes were determined. The result clearly demonstrated that the trophocyte at 36 h was covered with 4 layers, and then outer layer of the sporocyte gradually degraded over time, resulting in wall structure consisting of two layers, with even processes being detached from 7-day-old sporangium with smooth surface, indicating that the difference in the number of layers seems not to be an appropriate ultrastructural character for distinguishing P. infectans and P. sinerae. While pairwise comparison of the large subunit rDNA sequences showed 100% identity among P. infectans / P. sinerae species complex, genetic differences were found in the small subunit (SSU) rDNA sequences but the differences were relatively small (11-13 nucleotides) compared with those (190-272 nucleotides) found among the rest of Parvilucifera species (P. rostrata and P. corolla). Those small differences in SSU rDNA sequences of P. infectans / P. sinerae species complex may reflect the variations within inter- strains of the same species from different geographical areas. Taken together, all morphological, ultrastructural, and molecular data from the present study suggest that they are the same species.

• Animal Systematics, Evolution and Diversity
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• v.17 no.1
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• pp.141-144
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• 2001

A new species, Nazeris rutilicorpus, of the subfamily Paederinae is described in Korea. This species is found from dead leaves near streams or forests. The illustrations of labrum, male abdomen and genitalia are presented. The differences between N. rutilicorpus and N. optatus (Sharp) are discussed.

• Journal of Species Research
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• v.5 no.3
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• pp.498-502
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• 2016

Four new Korean records of the gammarid (Crustacea, Amphipoda), Grandidierella fasciata Ariyama, 1996; Moolapheonoides acutifalcatus Kobayashi and Ishimaru, 2005; Guernea terelamina Hirayama, 1985; and Gordonodius zelleri (Berge, Vader and Coleman, 1999) were reported from the Korean indigenous species survey project funded by the National Institute of Biological Resources (2014-2015). Each of these species is well accorded with the original description for the species except for some minor morphological differences. The genera Moolapheonoides Barnard, 1974 and Gordonodius Ariyama, 2011 are reported for the first time from Korea.

• Animal cells and systems
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• v.2 no.3
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• pp.309-313
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• 1998

We found some larvae of non-biting midges (Diptera: Chironomidae) that Injured rice crops by feeding on seeds and/or roots in the reclaimed rice fields in Seosan, Chungchongnam-do, Korea, in May 1997, Four instar larvae were collected and reared in the laboratory. This pest species of the rice plant was identified as a new species of Cricotopus, similar to C. sylvestris. The main differences are the color pattern of the abdominal tergites. Both adult and immature stages of the new species are described with illustrations.

• Mycobiology
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• v.29 no.2
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• pp.80-84
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• 2001

Nine species of genus Ganoderma different in cultural characteristics each other were separated by histo-anatomical differences. Differences among the species and geographic distribution of G. lucidum were also analyzed to provide the criteria for the classification of Korean cultivation or wild type strains. Korean cultivation and wild type strains were quiet different from other species as well as Taiwan and North American G. lucidum strains in histo-anatomical traits of Ganoderma. Pore color of Korean G. lucidum strains was less brown than those of Taiwan and North American strains. Shapes of pores were round or circular in Korean strains but ellipsoidal or angular in Taiwan or North American strains. Pore numbers of Korean strains were more than those($4{\sim}6/mm$) of Taiwan or North American strains. Hardness of the pileus of Korean strains was much more than that of Taiwan or North American strains. Such characteristics of Korean strains were different from those of other species of Ganoderma. Korean G. lucidum strains could be classified into the other group because they had many different traits in growth characteristics of fruiting bodies and histo-anatomical characteristics from those of Taiwan or North American G. lucidum and other species of G. lucidum complex.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.6
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• pp.732-739
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• 2011

Marine algal flora and community structure were examined at four sites at Tongyeong, Korea, from February to December 2009. Eighty-two algal species were identified, including 10 green, 25 brown, and 47 red algae. During the study period, the number of species was highest at the Dukpo (61 species) site followed by the Gajodo (60 species), Yepo (58 species), and Chilchundo (53 species) sites. Seaweed biomass ranged from 578.11 to 678.26 g, with the maximum and minimum at Chilchundo and Yepo, respectively. Dominant species in term of biomass were Sargassum thunbergii at Yepo, Dukpo, and Gajodo and Gelidium amansii at Chilchundo. The vertical distribution from the high to low intertidal zone was Sargassum thunbergii and Chondria crassicaulis; Sargassum thunbergii and Ulva linza; Ulva pertusa, Sargassum fulvellum, and Gelidium amansii. Two groups clearly segregated in a cluster analysis, a Yepo/Dukpo group (group A) and a Gajodo/Chilchundo (B), indicating relatively strong differences in similarity. The seaweed community structure of group A was characterized by higher biomass and higher species richness of brown algae than were observed in group B. Additionally, the biomass ratio and the species richness of green algae was lower in group A. These differences in seaweed community structure may have been resulted from the effects of cold effluents.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.55 no.5
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• pp.742-750
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• 2022

This study aimed to monitor the distribution of Cobitidae in Korea by the identification of species using genetic analysis. Based on the genetic analysis, Cobitidae species in four of five domestic fish farms consisted of only Chinese muddy loach Misgurnus mizolepis, but muddy loach Misgurnus anguillicaudatus was also present it in one fish farm. In the case of imported Cobitidae species, in addition to Chinese muddy loach and muddy loach, the harmful species Paramisgurnus dabryanus, was also present. Chinese muddy loach accounted for 20%, 67%, and 60% of the S6, S7, and S8 samples, respectively. An analysis of the total length, body length, and weight showed that domestic Chinese muddy loach showed higher values than imported muddy loach, and imported Chinese muddy loach showed similar values to P. dabryanus. There were no significant differences in the country of origin of the three species. Thus, the mitochondrial cytochrome c oxidase subunit I gene sequence was analyzed and compared the verification of species identification. The three species of Cobitidae were genetically divided into three groups and determined to have genetic differences. These results indicate that it is necessary to reduce the heterogeneous mixing rate through discriminating species by genetic analysis.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.3
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• pp.355-362
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• 2017

Objective: The use of locally produced forage (LPF) in cattle production has economic and environmental advantages over imported forage. The objective of this study was to characterize the nutritional value of LPF commonly used in Korea. Differences in ruminal fermentation characteristics were also examined for the LPF species commonly produced from two major production regions: Chungcheong and Jeolla. Methods: Ten LPF (five from each of the two regions) and six of the most widely used imported forages originating from North America were obtained at least three times throughout a year. Each forage species was pooled and analyzed for nutrient content using detailed chemical analysis. Ruminal fermentation characteristics were also determined by in vitro anaerobic incubations using strained rumen fluid for 0, 3, 6, 12, 24, and 48 h. At each incubation time, total gas, pH, ammonia, volatile fatty acid (VFA) concentrations, and neutral detergent fiber digestibility were measured. By fitting an exponential model, gas production kinetics were obtained. Results: Significant differences were found in the non-fiber carbohydrate (NFC) content among the forage species and the regions (p<0.01). No nutrient, other than NFC, showed significant differences among the regions. Crude protein, NFC, and acid detergent lignin significantly differed by forage species. The amount of acid detergent insoluble protein tended to differ among the forages. The forages produced in Chungcheong had a higher amount of NFC than that in Jeolla (p<0.05). There were differences in ruminal fermentation of LPF between the two regions and interactions between regions and forage species were also significant (p<0.05). The pH following a 48-h ruminal fermentation was lower in the forages from Chungcheong than from Jeolla (p<0.01), and total VFA concentration was higher in Chungcheong than in Jeolla (p = 0.05). This implies that fermentation was more active with the forages from Chungcheong than from Jeolla. Analysis of gas production profiles showed the rate of fermentation differed among forage species (p<0.05). Conclusion: The results of the present study showed that the nutritional values of some LPF (i.e., corn silage and Italian ryegrass) are comparable to those of imported forages widely used in Korea. This study also indicated that the nutritional value of LPF differs by origin, as well as by forage species. Detailed analyses of nutrient composition and digestion kinetics of LPF should be routinely employed to evaluate the correct nutritional value of LPF and to increase their use in the field.

• Korean Journal of Plant Taxonomy
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• v.45 no.2
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• pp.145-157
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• 2015

The nucleotide sequences of nuclear ribosomal ITS regions and chloroplast rbcL, matK and psbA-trnH regions of 30 individuals of Dendrobium moniliforme from several localities in four countries and 28 related species of Dendrobium were compared to investigate the genetic differences among Korean, Japanese, Taiwanese and Chinese D. moniliforme, and to verify the homogeneity of D. moniliforme, which is used as a traditional medicine in East Asia. A phylogenetic analysis showed that Korean D. moniliforme and Japanese D. moniliforme form a monophyletic group, with no significant differences between their nucleotide sequences. This confirms that they are the same species. However, the Chinese and Taiwanese D. moniliforme were polyphyletic. Various species related to D. moniliforme were located between the Korean-Japanese D. moniliforme and the Chinese-Taiwanese D. moniliforme, and other related species were found between individuals of Chinese-Taiwanese D. moniliforme. D. moniliforme is described in Japan, providing evidence that the Korean-Japanese D. moniliforme is the original species. In addition, our data suggest that the Chinese-Taiwanese D. moniliforme complex is a mixture of a range of other species. Further studies are required to understand the taxonomic identity of this species. In the Korean-Japanese D. moniliforme, there were almost no genetic differences among the localities, whereas the genetic heterogeneity was high among individuals of the Chinese-Taiwanese D. moniliforme.

• Applied Microscopy
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• v.43 no.1
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• pp.21-26
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• 2013

The oriental fruit moth (Grapholita molesta Busck) and the peach fruit moth (Carposina sasakii Matsumura) are the most severe insect pests affecting apple orchards in Korea. To prevent an outbreak of these two species and to control these agricultural insect pests, it is important to identify them accurately. However, it is hard to classify them when they were in the larval stage since they tunnel into the apple fruit. In this study, surface structures of the two species of larvae were observed using stereo microscope and scanning electron microscope. Distinct differences between the two species of larvae were found. The prothorax spiracles of oriental fruit moth larvae were approximately twice as large as those of peach fruit moth larvae. The arrangements of subventral setae, located around the proleg, were different between oriental fruit moth and peach fruit moth larvae. Furthermore, subdorsal setae of oriental fruit moth were located next to the spiracle on the 8th abdominal segment, while that of peach fruit moth was located above the spiracle. The identification of the two species of larvae observed in this study was confirmed using polymerase chain reaction-restriction fragment length polymorphism method. Surface structural differences are intrinsic characteristics for each species of larvae and can easily be identified using stereo microscope. These specificities will be helpful where a large number of field-collected larvae need to be identified routinely in pest control research.