• Korean Journal of Fisheries and Aquatic Sciences
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• v.51 no.3
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• pp.230-237
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• 2018

This study investigated the food and nutritional characteristics of alkaline-insoluble fractions (AIFs) recovered from bastard halibut Paralichthys olivaceus (BH) and skipjack tuna Katsuwonus pelamis (ST) roes using the alkaline solubilization. The moisture content of AIFs ranged from 4.8% to 12.8%, and ST provided significantly better yields (9.5 for STAIF-11 and 7.1 g/100 g roe for STAIF-12) than did BH (P<0.05). The protein content of AIFs ranged from 71.7% to 79.2%, with the highest level yielded by STAIF-11 (6.8 g/100 g roe). The crude fat content of AIFs was 10.9-14.3% and the mineral content was 0.7-3.4%. The major mineral components of AIFs were sulfur, sodium, potassium, and phosphorus. Color values showed that BHAIFs were significantly brighter than STAIFs. Total contents of essential amino acids were significantly higher in STAIFs (47.5-49.5%) than in BHAIFs. The major essential amino acids found in AIFs from both sources were Val, Leu, Lys, and Arg. Therefore, AIFs were significantly superior to whole BH roe in terms of physicochemical and nutritional status, and we identified species-specific differences between BH and ST. Protein is a major component of AIFs recovered from fish roes, which suggests that they have potential for use as a protein source.

• Fisheries and Aquatic Sciences
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• v.23 no.8
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• pp.20.1-20.18
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• 2020

Background: Olive flounder, Paralichthys olivaceus, is an economically important aquaculture species in Korea. Olive flounders have been heavily damaged by streptococcal infections every year and are treated with antibiotics. However, antibiotic abuse is causing the emergence of resistant strains, and to overcome this, research has shown that new antibiotics must be applied. Tylosin is a relatively safe antibiotic and has good activity against Gram-positive bacteria and mycoplasma. We studied the therapeutic effects and side effects of tylosin on Streptococcus parauberis-infected olive flounder. Methods: After artificial infection of olive flounder with S. parauberis SPOF18J3, an appropriate dose of tylosin was confirmed by intramuscular injection (I.M.) at 2.5, 5, 10, and 15 mg/kg, and oral administration at 10 and 20 mg/kg. After I.M. and oral administration dosing of tylosin, side effects were confirmed by serological analysis, histopathological analysis, and median lethal dose (LD₅₀) analysis at both an appropriate concentration and a high concentration. Statistical analysis was performed using one-way analysis of variance (ANOVA) and Tukey's test (p < 0.05). Results: The appropriate I.M. and oral administration concentration of tylosin administered to olive flounder infected with S. parauberis SPOF18J3 was found to be 10 mg/kg. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were showed not significantly different between the control group and the experimental groups. The histopathologic results showed mild inflammatory responses in muscle and tubular vacuolization and tubular atrophy appeared, but there were no significant differences between the groups. The LD₅₀ was confirmed to be 461 mg/kg. Conclusion: In this study, an effective treatment method was provided by verifying the treatment effects and side effects of tylosin in olive flounder infected with S. parauberis, which can be applied directly to aquaculture sites. In addition, these results may be used as a reference for evaluation required upon request to obtain approval for tylosin antibiotics as fishery antibiotics in Korea. After approval, it is possible that a fishery disease manager will be able to prescribe and sell the antibiotic tylosin.

• Journal of Korean Society of Forest Science
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• v.77 no.2
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• pp.186-192
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• 1988

This study was carried out to estimate the stand form factor that is necessary to estimate stand volume by STRAND method among plotless sampling methods. The data measured for this study were based on the 380 sample plots from Larix leptolepis and 358 sample plots from Pinus koraiensis which were distributed in the region of Kyeongi, Kangweon, Chungbuk, Chungnam, Chun-buk and Kyeongbuk. 1. Stand form factor was highly correlated with in the following order, stand form height, stand average height, average diameter, stand age, distance of stem, and basal area height. 2. The best fitted equation of stand form factor of above two tree species were presented in table 3. 3. Stand form factor tables using estimated equations on the table 3 were prepared and presented in table 4, 5(Larix leptolepis), 6, 7(Pinus karaiensis). 4. The relatioinships between estimated value and actual value were Y=bx, where b approached nearly 1.0, and there were not any significant differences between them. 5. The percentages of estimated error on stand form factor table were ranged from 2.39% to 4.15% in Larix leptolepis and from 1.73% to 2.50% in Pinus koraiensis. Therefore, the stand form factor could be exactly estimated by use of these tables.

• KSBB Journal
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• v.27 no.5
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• pp.308-312
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• 2012

Argon-plasma jet (Ar-PJ) is generated by ionizing Ar gas, and the resulting Ar-PJ consists of a mixture of neutral particles, positive ions, negative electrons, and various reactive species. Although Ar-PJ has been used in various biomedical applications, little is known about the biological effects on cells located near the plasma-exposed region. Here, we investigated the effects of the Ar-PJ on actin cytoskeleton of mouse embryonic fibroblasts (MEFs) in response to indirect as well as direct exposure to Ar-PJ. This Ar-PJ was generated at 500 mL/min of flow rate and 100 V electric power by our device mainly consisting of electrodes, dielectrics, and a high-voltage power supply. Because actin cytoskeleton is the key cellular machinery involved in cellular movement and is implicated in regulation of cancer metastasis and thus resulting in a highly desirable cancer therapeutic target, we examined the actin filament architectures in Ar-PJ-treated MEFs by staining with an actin-specific phalloidin labeled with fluorescent dye. Interestingly, the Ar-PJ treatment causes destabilization of actin filament architectures in the regions indirectly exposed to Ar-PJ, but no differences in MEFs treated with Ar gas alone and in untreated cell control, indicating that this phenomenon is a specific cellular response against Ar-PJ in the live cells, which are indirectly exposed to Ar-PJ. Collectively, our study raises the possibility that Ar-PJ may have potential as anti-cancer drug effect through direct destabilization of the actin cytoskeleton.

• Journal of Korean Society of Forest Science
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• v.82 no.3
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• pp.246-253
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• 1993

The composition of the nitrogen forms of the organic layer and mineral soil horizons of 8 differing soil type was measured to find a clue to elucidate the problem on the fertility and nitrogen availabilities of forest soils. The ratio of inorganic $N(NH_4-N+NO_3-N)$ to total N was very low in every 0 layer and its maximum was only about 2%. And the inorganic N concentration of every mineral soil horizon was low, and the maximum of its rate to N was 3.7% in surface horizon and about 4.1% in lower horizon. Organic N form of O layer were characterized by the decrease of aminoacid-N, and the increased of hexosamine-N and amide-N according to the advance of decomposing process. The ranges of hydrolyzable-N of every flesh litter, F and H layer of the selected soils were about 80-90% and they- were hardly affected by the differences of tree species and decomposing process. The organic N form of every litter was similar and it was follows : aminoacid-N was not abundant and it ranges were about 40-50%, more than half of hydrolyzable-N. The hexosamine-N was eery poor and it ranged to only 2-7%. The amide-N teas low and about 10-23%. No distinguished difference was seen between the organic N forms of the O layer and surface horizon of dry and wet soils in brown forest soils.

• Journal of Korean Society of Forest Science
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• v.95 no.3
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• pp.261-267
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• 2006

A nursery trial comprising 109 progenies of 5 populations of Cornus kousa was analysed using multivariate analysis of 12 quantitative traits (growth and leaf form). The aim of this study was to examine geographic variation of the species based on the single, existing nursery trial to support a genetic resource conservation plan of Cornus kousa in Korea. Nested Anova showed that there were statistically significant differences among populations as well as among families within populations in all 12 quantitative traits. In 10 of 12 traits, variance components among families within populations were higher than those among populations. Cluster analysis using complete linkage method showed three groups to Euclidean distance 0.8. Among principal components, primary 2 principal components appeared to be major variables because of the loading contribution of 91.9%. The first contribution component was maximum width, vein number, blade length/petiole length and upper 1/3 width lower 1/3 width; the second one was height, diameter at root collar, blade length, upper 1/3 width, petiole length and petiole length/vein number, respectively. But all characters showed no significant difference with the pattern of geographic distribution.

• Journal of Korean Society of Forest Science
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• v.55 no.1
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• pp.1-21
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• 1982

The pollen morphology of 2 forms, 6 varieties, 50 species, 15 genera, 11 families of Korean Sapindales was studied. The keys were provided for general and families of Korean Sapindales. General pollen morphology was diversed and taxonomically significant differences were obtained in shape, aperture, type and size, sculpture pattern and exine thickness of pollen grains. Pollen grains of Korean Sapindales could be grouped into four types and five subtypes. One of five sub-types pollen grains of Acer could be ground into four under subtypes: ginnala type, barbinerve type, psseudo-sieboldianum type and negundo type. Pollen morphology of Empetraceae, Buxaceae and Balsaminaceae appeared to have very special features in the equatorial view and aperature type compare with pollen grain of the other families of Korean Sapindales.

• Journal of Korean Society of Environmental Engineers
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• v.37 no.4
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• pp.197-203
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• 2015

Particulate matter (PM) has emitted in many regions of the world and is causing many health-related problems. Thus reasonable politics and solutions are needed to reduce PM in Seoul. Further it is required to clearly explain the major portions of chemical components contained in $PM_{10}$ to figure out the characteristics of $PM_{10}$, and to develop effective reduction measures in order to decrease the adverse effects of $PM_{10}$. $PM_{10}$ samples were collected in Seoul and analyzed their ions to examine the physical and chemical characteristics of ionic species. Since hydrogen ion ($H^+$) and carbonate ion (${CO_3}^{2-}$)) cannot be analyzed by Ion chromatography (IC), concentrations of $H^+$ and ${CO_3}^{2-}$ were initially estimated by pH and equivalent differences between anions and cations in this study. Starting from the study findings, good combination results for compositional patterns between anions and cations were obtained by applying a mathematical modelling technique that was based on the mass balance principle. The ions in $PM_{10}$ were combined with $H^+$, ${CO_3}^{2-}$, and supplement for $NO_3{^-}$, $Cl^-$ formed such compounds $NH_4Cl$, $NH_4NO_3$, $CaSO_4$, $(NH_4)_2SO_4$, $NaNO_3$, NaCl, $Na_2CO_3$, and $(NH_4)_2CO_3$ in the study area.

• Journal of Korean Society for Atmospheric Environment
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• v.23 no.6
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• pp.675-688
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• 2007

To characterize organic and elemental carbon (OC and EC), and water-soluble organic carbon (WSOC) contents, daily $PM_{2.5}$ measurements were performed in August 2006 (summer) and Jan $11{\sim}Feb$ 12 2007 (winter) at an urban site of Gwangju. Daily size-segregated aerosol samples were also collected for WSOC analysis. No clear seasonal variations in EC and WSOC concentrations were observed, while seasonal differences in OC concentration, and OC/EC and WSOC/EC ratios were shown. The WSOC/OC ratio showed higher value in summer (0.56) than in winter (0.40), reflecting the greater enhancement of secondary WSOC formation at the site in summer. Secondary WSOC concentrations estimated using EC tracer method were in the range $0.0{\sim}2.1\;{\mu}g/m^3$ (average $0.42\;{\mu}g/m^3$) and $0.0{\sim}1.1\;{\mu}g/m^3\;(0.24\;{\mu}g/m^3)$, respectively, accounting for $0{\sim}51.6%$ (average 16.8%) and $0{\sim}52.5%$ (average 13.1 %) of the measured WSOC concentrations in summer and winter. Sometimes higher WSOC/OC ratio in winter than that in summer could be attributed to two reasons. One is that the stable atmospheric condition often appears in winter, and the prolonged residence time would strengthen atmospheric oxidation of volatile organic compounds. The other is that decrease of ambient temperature in winter would enhance the condensation of volatile secondary WSOC on pre-existing aerosols. In summertime, atmospheric aerosols and WSOC concentrations showed bimodal size distributions, peaking at the size ranges $0.32{\sim}0.56\;{\mu}m$ (condensation mode) and $3.2{\sim}5.6\;{\mu}m$ (coarse mode), respectively. During the wintertime, atmospheric aerosols showed a bimodal character, while WSOC concentrations showed a unimodal pattern. Size distributions of atmospheric aerosols and WSOC with a peak in the size range $0.32{\sim}0.56\;{\mu}m$ were observed for most of the measurement periods. On January 17, however, atmospheric aerosols and WOSC exhibited size distributions with modal peaks in the size range $1.0{\sim}1.8\;{\mu}m$, suggesting that the aerosol particles collected on that day could be expected to be more aged, i.e, longer residence time, than the aerosols at other sampling periods.

• The Korean Journal of Pesticide Science
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• v.16 no.4
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• pp.336-342
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• 2012

This study was carried out in order to compare the residue levels of pesticides among lettuce, kale and perilla leaf depending on the morphological characteristics of plant. Residue levels were investigated at the zero, second, fifth days after last application, 9 species of systemic or non-systemic pesticide were twice applied with 7 days interval by knapsack power sprayer with 2 heads fan shape nozzle. Ratios of leaf area to weight ($cm^2$ $g^{-1}$) were 58 of perilla leaf, 27 of lettuce and 23 of kale. Ratios of leaf area to weight of perilla leaf was 2.1 times higher than that of lettuce. Residue levels of perilla leaf were 1.3 to 2.3 times higher than those of lettuce at the day of spraying and 1.3 to 3.3 times higher at the fifth day. Therefore the differences of pesticide residues between perilla leaf and lettuce were affected by the ratio of leaf area to weight. Residue levels in lettuce were 2.4 to 7.3 times higher than those in kale at the day of spraying because the adhesive effect of pesticide particles on kale leaf was low.