Browse > Article
http://dx.doi.org/10.7841/ksbbj.2012.27.5.308

Effects of Argon-plasma Jet on the Cytoskeleton of Fibroblasts: Implications of a New Approach for Cancer Therapy  

Han, Ji-Hye (Department of Medical Life Sciences, Department of Biomedical Sciences, College of Medicine, the Catholic University of Korea)
Nam, Min-Kyung (Department of Medical Life Sciences, Department of Biomedical Sciences, College of Medicine, the Catholic University of Korea)
Kim, Yong-Hee (Plasma Bioscience Research Center, Kwangwoon University)
Park, Dae-Wook (Department of Medical Life Sciences, Department of Biomedical Sciences, College of Medicine, the Catholic University of Korea)
Choi, Eun Ha (Plasma Bioscience Research Center, Kwangwoon University)
Rhim, Hyangshuk (Department of Medical Life Sciences, Department of Biomedical Sciences, College of Medicine, the Catholic University of Korea)
Publication Information
KSBB Journal / v.27, no.5, 2012 , pp. 308-312 More about this Journal
Abstract
Argon-plasma jet (Ar-PJ) is generated by ionizing Ar gas, and the resulting Ar-PJ consists of a mixture of neutral particles, positive ions, negative electrons, and various reactive species. Although Ar-PJ has been used in various biomedical applications, little is known about the biological effects on cells located near the plasma-exposed region. Here, we investigated the effects of the Ar-PJ on actin cytoskeleton of mouse embryonic fibroblasts (MEFs) in response to indirect as well as direct exposure to Ar-PJ. This Ar-PJ was generated at 500 mL/min of flow rate and 100 V electric power by our device mainly consisting of electrodes, dielectrics, and a high-voltage power supply. Because actin cytoskeleton is the key cellular machinery involved in cellular movement and is implicated in regulation of cancer metastasis and thus resulting in a highly desirable cancer therapeutic target, we examined the actin filament architectures in Ar-PJ-treated MEFs by staining with an actin-specific phalloidin labeled with fluorescent dye. Interestingly, the Ar-PJ treatment causes destabilization of actin filament architectures in the regions indirectly exposed to Ar-PJ, but no differences in MEFs treated with Ar gas alone and in untreated cell control, indicating that this phenomenon is a specific cellular response against Ar-PJ in the live cells, which are indirectly exposed to Ar-PJ. Collectively, our study raises the possibility that Ar-PJ may have potential as anti-cancer drug effect through direct destabilization of the actin cytoskeleton.
Keywords
Argon-plasma jet; Cancer therapy; Actin cytoskeleton;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kim, S. H., J. H. Kim, B. K. Kang, and H. S. Uhm (2005) Superhydrophobic CFx coating via in-line atmospheric RF plasma of He-CF4-H2. Langmuir. 21: 12213-12217.   DOI
2 Kilmer, S., N. Semchyshyn, G. Shah, and R. Fitzpatrick (2007) A pilot study on the use of a plasma skin regeneration device (Portrait PSR3) in full facial rejuvenation procedures. Lasers Med. Sci. 22: 101-109.   DOI
3 Potter, M. J., et al. (2007) Facial acne and fine lines: transforming patient outcomes with plasma skin regeneration. Ann. Plast Surg. 58: 608-613.   DOI
4 Bogle, M. A., K. A. Arndt, and J. S. Dover (2007) Evaluation of plasma skin regeneration technology in low-energy full-facial rejuvenation. Arch. Dermatol. 143: 168-174.   DOI
5 Grund, K. E., D. Storek, and G. Farin (1994) Endoscopic argon plasma coagulation (APC) first clinical experiences in flexible endoscopy. Endosc. Surg. Allied Technol. 2: 42-46.
6 Klein, C. A. (2008) Cancer. The metastasis cascade. Science 321: 1785-1787.   DOI
7 Chiang, A. C. and J. Massague (2008) Molecular basis of metastasis. N. Engl. J. Med. 359: 2814-2823.   DOI
8 Kedrin, D., J. van Rheenen, L. Hernandez, J. Condeelis, and J. E. Segall (2007) Cell motility and cytoskeletal regulation in invasion and metastasis. J. Mammary Gland Biol. Neoplasia 12: 143-152.   DOI
9 Rhodes, L. V., et al. (2011) Cytokine receptor CXCR4 mediates estrogen-independent tumorigenesis, metastasis, and resistance to endocrine therapy in human breast cancer. Cancer Res. 71: 603-613.   DOI
10 Entschladen, F., Drell, T. L. t., Lang, K., Joseph, J., and Zaenker, K. S. (2004) Tumour-cell migration, invasion, and metastasis: navigation by neurotransmitters. Lancet Oncol. 5: 254-258.   DOI   ScienceOn
11 Olson, M. F. and E. Sahai (2009) The actin cytoskeleton in cancer cell motility. Clin. Exp. Metastasis 26: 273-287.   DOI
12 Nobes, C. D. and A. Hall (1995) Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia. Cell. 81: 53-62.   DOI   ScienceOn
13 Wennerberg, K. and C. J. Der (2004) Rho-family GTPases: it's not only Rac and Rho (and I like it). J. Cell Sci. 117: 1301-1312.   DOI
14 Fritz, G., C. Brachetti, F. Bahlmann, M. Schmidt, and B. Kaina (2002) Rho GTPases in human breast tumours: expression and mutation analyses and correlation with clinical parameters. Br. J. Cancer 87: 635-644.   DOI
15 Frixione, E. (2000) Recurring views on the structure and function of the cytoskeleton: a 300-year epic. Cell Motil Cytoskeleton 46: 73-94.   DOI
16 Jordan, M. A. and Wilson, L. (1998) Microtubules and actin filaments: dynamic targets for cancer chemotherapy. Curr Opin Cell Biol. 10: 123-130.   DOI   ScienceOn
17 Doherty, G. J. and H. T. McMahon (2008) Mediation, modulation, and consequences of membrane-cytoskeleton interactions. Annu. Rev. Biophys. 37: 65-95.   DOI
18 Fuchs, E. and D. W. Cleveland (1998) A structural scaffolding of intermediate filaments in health and disease. Science 279: 514-519.   DOI
19 Suresh, S. (2007) Biomechanics and biophysics of cancer cells. Acta Biomater 3: 413-438.   DOI
20 Sharma, S., et al. (2012) Correlative nanomechanical profiling with super-resolution F-actin imaging reveals novel insights into mechanisms of cisplatin resistance in ovarian cancer cells. Nanomedicine 8: 757-766.   DOI
21 Statsuk, A. V., et al. (2005) Actin is the primary cellular receptor of bistramide A. Nat. Chem Biol. 1: 383-388.   DOI
22 Hayot, C., et al. (2006) Characterization of the activities of actinaffecting drugs on tumor cell migration. Toxicol Appl Pharmacol. 211: 30-40.   DOI   ScienceOn
23 Coue, M., S. L. Brenner, I. Spector, and E. D. Korn (1987) Inhibition of actin polymerization by latrunculin A. FEBS Lett. 213: 316-318.   DOI   ScienceOn
24 Hemstreet, G. P., 3rd, et al. (1996) G-actin as a risk factor and modulatable endpoint for cancer chemoprevention trials. J. Cell Biochem. Suppl. 25: 197-204.
25 Baskar, R., K. A. Lee, R. Yeo, and K. W. Yeoh (2012) Cancer and radiation therapy: current advances and future directions. Int. J. Med. Sci. 9: 193-199.   DOI
26 DeVita, V. T., Jr. and E. Chu (2008) A history of cancer chemotherapy. Cancer Res. 68: 8643-8653.   DOI