• 한국가축번식학회지
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• 제7권2호
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• pp.8-26
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• 1983

Various early pregnancy diagnostic methods have been developed in order to improve the reproductive efficiency in cow, mare, mule, sow, sheep, goat, dog, cat, rabbit, buffalo, camel, elephant, monkey, deer, lion, coipus and guinea pig. These methods include abdominal swelling, abdominal palpation, esturs cylce detection, Lupin test, gonadotropin assay, colostrum injection test, sperm motility assessment, cervical mucus viscosity test, Kaber chromagens method, estrogen test, A Scheim-Zond다 test, spectrophotometric detection of estrogen in urine and feces, boric acid crystraline formation test in urine, oxytocin injection test, diamino-oxidase test, PMSG HA test, behaviour test, Simolus iodine detection test, detection of tryptophane in urine, x-ray method, Cuboni and Lunaas method, vaginal biopsy method, Friedmann Schneider diagnostic method, electrode method, barium chloride detection method, ECG, Doptone method, ultrasound method, ultrasound scanning method, LDH method, rectal palpation method, CL palpation method, radioautography, serum creatine test, serum globulin test, chlormadine method, CAP method, Medata Do, pp.ers method, body fluid test, Plasma oCS detection method, ERIA, LHRH method, negative latex cogulation test and oestrone sulphate detection method. The most reliable methods with high a, pp.icability to farm animals such as sheep, mare, sow and cow are rectal palpation, ultrasound method and hormonal assay in blood and milk. However, they require complicated laboratory works for the early diagnosis of pregnancy and in most cases, the simple and economical methods which are described up to now need a long period of time after conception. Generally, it is possible to detect pregnancy after one estrus cycle, even though it varies depending on the species of animals. For improvement of the reproductive efficiency, it is required to develop a more accurate, economical, simple and early detectable method. It is anticipated that the result of a study on the detection method of EPF(early pregnancy factor) would be a, pp.icable to various animals within 6 hours after conception.

• 한국어병학회지
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• 제26권2호
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• pp.99-110
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• 2013

수산종묘는 인류에게 중요한 동물성 단백질을 제공할 뿐만 아니라, 방류를 통한 수산자원 관리의 측면에서도 매우 중요하다. 수산자원의 증강을 목적으로 2009년에는 33품종, 2010년에는 44품종, 2011년에는 43품종, 2012년에는 46품종에 대해 수산생물전염병에 대한 감염 여부를 조사하였다. 검사품종 중에서 해면품종으로는 전복이 가장 많았으며, 그 다음 해삼, 넙치, 조피볼락, 꽃게 순으로 나타났다. 내수면품종 중에서는 붕어가 가장 많았으며, 그 다음으로 쏘가리, 대농갱이, 다슬기, 참게의 순으로 나타났다. 감성돔, 돌돔, 붉은쏨뱅이, 뱀장어는 4년 동안 지속적으로 검사 횟수가 감소하였다. 숭어에 대해서는 2009년에만 전염병 검사가 수행되었다. 8개의 검사 항목에 대해 총 8,476건의 검사가 실시되었으며, 56건 (0.67%)에서 koi herpesvirus, white spot syndrome virus, red sea bream iridovirus 또는 viral haemorrhagic septicemia virus와 같은 병원체가 검출되어 불합격 처리되었다.

• 미생물학회지
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• 제43권2호
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• pp.106-110
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• 2007

USEPA 1605 방법을 이용하여 서울시 상수도계통에서 Aeromonas를 조사하였다. 2002년 7월부터 2003년 12월까지 매월 한강수계 하천수와 서울시 정수장에서 공급되는 정수, 수돗물에서 시료를 채취하였다. Aeromonas는 선택배지(ADA-V)를 사용하여 membrane 필터 표면에 성장한 노란색 집락을 계수하여 측정하였다. 하천수에서는 Aeromonas가 $1.0{\times}10^{0}-9.8{\times}10^{3}\;CFU/ml$의 농도로 검출되었으며, 정수에서는 검출되지 않았다. 수돗물에서는 141개 중 1개의 시료에서 1 CFU/500 ml의 농도로 검출되었다. 확인된 Aeromonas는 대부분 비병원성인 A. salmonicida(51%)였고, 이 외에도 A. caviae(4.7%), A. schubertti(3.4%), A. sobria(3.8%), A. hydrophila(2.1%), A ichithiosmia(0.4%)등이 동정되었다. A. salmonicida에 대한 염소 저항성을 평가한 결과, 0.2 mg/L 염소농도에서 30초 접촉 후, 99.99% (일부)가 제거되었다. USEPA 1605 방법에서 제시한 정도관리를 수행한 결과, 정도관리 허용기준을 만족하였다. 본 연구를 통해 서울시 상수도계통에서 Aeromonas에 대한 안전성이 확보되었다.

• Food Science and Biotechnology
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• 제15권5호
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• pp.778-780
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• 2006

High performance liquid chromatography (HPLC) was used for the analysis of the lignans eleutheroside B and eleutheroside E in the fruits of Acanthopanax species. A reverse-phase system using a gradient of $H_2O$ and acetonitrile as the mobile phase was developed and detection was at 210 nm. The analysis was successfully carried out within 20 min. The content of eleutheroside B and eleutheroside E in Acanthopanax species was measured in the fruits of A. senticosus (0.58 and $1.66\;{\mu}g/mg$, respectively), A. sessiliflorus (1.15 and $8.49\;{\mu}g/mg$, respectively), A. koreanum (2.16 and $1.80\;{\mu}g/mg$, respectively), and A. divaricatus (1.06 and $7.08\;{\mu}g/mg$, respectively).

• Journal of Ecology and Environment
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• 제41권3호
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• pp.72-77
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• 2017

The world of technology is pleasantly evolving to a stage where small robotic aid may be used to ease the work of researchers, and to one day bring more accurate results than the current human abilities allow. In the research field of species monitoring in biology, unmanned aerial vehicles (UAVs) have begun to play an important role in how research is approached, analyzed, and then applied for further investigation, particularly by focusing on a single species. This paper uses data that has been collected from June to October 2015, to demonstrate how the innovative idea of using UAVs to monitor a particular species will bring a positive development in conservation research, and what it was able to achieve in this research field so far. More precisely, we examine the potential of UAVs to take center stage in future research, as well as their current accuracy. This paper describes the use of the commercially available Phantom 2 Vision+ for the detection, assessment, and monitoring of the butterfly species Libythea celtis, demonstrating how it can help the monitoring of butterflies and how it could be developed for even more adventurous and detailed research in the future.

• Bulletin of the Korean Chemical Society
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• 제27권5호
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• pp.694-698
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• 2006

A method has been described for the chemical speciation, preconcentration and determination of Cr(III) and Cr(VI) species in filtered tannery waste waters by flame atomic absorption spectrometry using ion-exchange resins. Amberlite IR-120($H^+$) strongly acidic cation exchanger and Amberlite IRA-410($CI ^-$) strongly basic anion exchanger resins were used for the separation and preconcentration of Cr(III) and Cr(VI) species, respectively. Optimum condition for preconcentration and speciation was obtained by testing pH of sample and eluent, flow rates of sample and eluent, amount of resins, volume of sample and eluents, and effect of foreign ions. The recommended method has been successfully applied for the preconcentration and determination of chromium species in the dissolved phase of waste water samples collected from a tannery waste water treatment plant in Kayseri, Turkey. The detection limits achieved were 0.73 $\mu$g/L for Cr(III) and 0.81 $\mu$g/L for Cr(VI). Recovery studies showed 99% for Cr(III) and 98% for Cr(VI), for samples spiked with single species.

• 대한의생명과학회지
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• 제18권2호
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• pp.152-159
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• 2012

Campylobacter species are known to the high optimum growth temperature ($42^{\circ}C$) and the cause of enteritis in people. Erythromycin has a curative effect for enteritis caused by the bacteria. However, the rate of erythromycin-resistant bacteria was not well known until recently in Korea. Swine are one of sources of the infection with a Campylobacter species which cause the symptom of a high temperature. In this study, we cultured rectum fecal specimens of 100 pigs in an area of Buan-gun, Jeonbuk Province during July 2009. As a result, the detection rate of C. jejuni and C. coli and the rate of erythromycin-resistant bacteria for the separated Campylobacter species on the condition of high temperature were investigated. The possession or not of hipO and glyA gene and ciprofloxacin-resistant gene gyrA was also reviewed with biochemical characteristics and PCR.

• 한국동물위생학회지
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• 제24권2호
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• pp.133-138
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• 2001

Cryposporidium species have a wide host range. These coccidian parasites are found in close association with epithelial cells of many species of animals including mm. The gastrointestinal tract is most commonly affected in young ruminants and this parasite is thought to be considerable importance in calf diarrhea complex. Major outbreaks of cryptosporidiosis have been reported in calves, lambs, pigs and others including avian species. Cryptosporidiosis is transmitted by oocysts of Cryptosporidium species. Because cryptosporidiosis is common infection among animals, early literature considered it a zoonosis. Human infections contracted from calves, cats, and horse feces. However, the resrvoir host is longer considered the major source of infection. Mild cases of disease have been reported in farm workers. Immunosuppressed, very young and very old persons should avoid contact with this parasite because it may cause severe diarrhea. In order to detect of Crytosporidium sp infection from feces of cattle and pigs at Chonbuk Iksan and Kunsan area, sedimentation and modified acid fast stain were applied. The positive rate of Cryptosporidium sp infection from 1, 176 of cattle and 267 of pigs were 0.5 % and 16.8%, respectively. According to area in Iksan and Kunsan, the positive rates were 0.4% and 0.9% from cattle, and 18.9% and 12.1% from pigs, respectively. In cattle, positive detection rate was 0.6% in milking cows but not in Korean cattle.

• Journal of Dairy Science and Biotechnology
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• 제33권4호
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• pp.253-262
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• 2015

The authentication and implications of misleading labeling in milk and dairy products is important to protect against cheating consumers from adulteration and to alert sensitive consumers to any undeclared potential allergens. This need to support milk and dairy products labeling has led to the development of specific analytical techniques for the analysis of milk and dairy products ingredients. Recently, several methods based on polymerase chain reaction (PCR), including restriction fragment length polymorphism (PCR-RFLP), multiplex PCR, species-specific PCR, and real-time PCR, have been proposed as useful means for identifying species of origin in milk and dairy products, as well as quantifying and detecting any adulteration. These methods have particular advantages owing to their high specificity and sensitivity, as well as rapid processing time. In this review, we provide an updated and extensive overview of the PCR-based methods used for milk and dairy products authentication with a particular focus on the application of PCR methods to detect adulteration.

• 한국연소학회지
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• 제12권4호
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• pp.1-7
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• 2007

Carbon monoxide (CO) is not only an important intermediate species in chemical reaction mechanisms of hydrocarbon fuel combustion, but also a crucial pollutant species emitted from automotive engines. To better understand the physical processes impacting CO emissions, the development of laser-based measurement techniques that can visualize in-cylinder CO distributions is desirable. Among these techniques, Laser-Induced Fluorescence (LIF) is a sensitive and species-selective detection technique capable of good spatial resolution. However, some technical matters such as deep UV excitation, severe pressure dependency of the LIF signal, and potential interference from other species have been major challenges for CO LIF application. This study is focused on investigating the feasibility of CO two-photon LIF in a direct-injection diesel engine operating at typical pressure and temperature conditions with commercial grade diesel fuel. Spectroscopic analysis shows that the CO fluorescence signal can be separated from $C_2$ Swan band or broadband fluorescence from PAHs when the signal is collected near 483 nm. The signal-to-noise ratio of CO LIF deteriorate rapidly as pressure is increased, following $P^{-1.49}$ which matches the theoretical signal pressure dependency.