• The Plant Pathology Journal
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• 제40권1호
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• pp.16-29
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• 2024

The Colletotrichum gloeosporioides species complex includes many phytopathogenic species, causing anthracnose disease on a wide range of host plants and appearing to be globally distributed. Seventy-one Colletotrichum isolates in the complex from different plants and geographic regions in Korea were preserved in the Korean Agricultural Culture Collection (KACC). Most of them had been identified based on hosts and morphological features, this could lead to inaccurate species names. Therefore, the KACC isolates were re-identified using DNA sequence analyses of six loci, comprising internal transcribed spacer, gapdh, chs-1, his3, act, and tub2 in this study. Based on the combined phylogenetic analysis, KACC strains were assigned to 12 known species and three new species candidates. The detected species are C. siamense (n = 20), C. fructicola (n = 19), C. gloeosporioides (n = 9), C. aenigma (n = 5), C. camelliae (n = 3), C. temperatum (n = 3), C. musae (n = 2), C. theobromicola (n = 2), C. viniferum (n = 2), C. alatae (n = 1), C. jiangxiense (n = 1), and C. yulongense (n = 1). Of these, C. jiangxiense, C. temperatum, C. theobromicola and C. yulongense are unrecorded species in Korea. Host plant comparisons showed that 27 fungus-host associations are newly reported in the country. However, plant-fungus interactions need to be investigated by pathogenicity tests.

• ALGAE
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• 제33권1호
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• pp.1-19
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• 2018

Members of the family Parviluciferaceae (Alveolata, Perkinsozoa) are the well-known dinoflagellate parasitoids along with Amoebophrya ceratii species complex and parasitic chytrid Dinomyces arenysensis and contain six species across three genera (i.e., Parvilucifera infectans, P. sinerae, P. rostrata, and P. corolla, Dinovorax pyriformis, and Snorkelia prorocentri) so far. Among Parvilucifera species, the two species, P. infectans and P. sinerae, are very similar or almost identical each other morphologically and genetically, thereby make it difficult to distinguish between the two. The only main difference between the two species known so far is the number of sporangium wall (i.e., 2 layers in P. infectans vs. 3 layers in P. sinerae). During sampling in Masan bay, Korea during the spring season of 2015, the dinoflagellate Akashiwo sanguinea cells infected by the parasite Parvilucifera were observed and this host-parasite system was established in culture. Using this culture, its morphological and ultrastructural features with special emphasis on the variation in the number of sporangium wall over developmental times, were investigated. In addition, the sequences of rDNA regions and ${\beta}-tubulin$ genes were determined. The result clearly demonstrated that the trophocyte at 36 h was covered with 4 layers, and then outer layer of the sporocyte gradually degraded over time, resulting in wall structure consisting of two layers, with even processes being detached from 7-day-old sporangium with smooth surface, indicating that the difference in the number of layers seems not to be an appropriate ultrastructural character for distinguishing P. infectans and P. sinerae. While pairwise comparison of the large subunit rDNA sequences showed 100% identity among P. infectans / P. sinerae species complex, genetic differences were found in the small subunit (SSU) rDNA sequences but the differences were relatively small (11-13 nucleotides) compared with those (190-272 nucleotides) found among the rest of Parvilucifera species (P. rostrata and P. corolla). Those small differences in SSU rDNA sequences of P. infectans / P. sinerae species complex may reflect the variations within inter- strains of the same species from different geographical areas. Taken together, all morphological, ultrastructural, and molecular data from the present study suggest that they are the same species.

• 대한임상검사과학회지
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• 제49권4호
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• pp.427-434
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• 2017

Acinetobacter calcoaceticus-baumannii (Acb) complex에 속한 종들은 빈번하게 병원감염 및 기회감염을 일으킨다. 또한 다제내성인 경우가 많아 이 균들의 감염증 치료를 위한 항균제 선택이 매우 제한적이다. 본 연구에서는 ciprofloxacin 내성 Acinetobacter species 53균주를 대상으로 fluoroquinolone 내성기전을 조사했다. 항균제 감수성 양상을 조사하기 위해 디스크확산법이 시행되었다. Fluoroquinolone 내성과 관련된 유전자 및 돌연변이 검출을 위해 PCR과 염기서열분석이 이루어졌다. 본 연구에서 수집된 53균주의 ciprofloxacin 내성 Acinetobacter 중 47균주가 gyrA 유전자의 83번째 serine 아미노산 잔기와 parC 유전자의 80번째 serine 아미노산 잔기가 leucine 잔기로 치환된 sense mutations 가지고 있는 것으로 나타났다. gyrA와 parC 유전자에 sense mutations을 가지고 있는 47균주 중 44균주가 A. baumannii 였고 3균주는 A. pittii였다. 본 연구에서 조사대상이 되었던 Acb complex 균주들 중 plasmid-mediated quinolone resistance (PMQR) determinants를 가지고 있는 균주는 한나도 없었다. 46 균주의 ciprofloxacin 내성 A. baumannii 는 A, B, 또는 F형의 banding pattern을 보였는데 이는 충청지역에 위치한 일개의 병원에 ciprofloxacin 내성 A. baumannii가 수평확산 되어 있음을 의미한다. Fluoroquinolone 내성 Acb complex 균주의 집락화 및 확산을 막기 위해서 다제내성 균주들을 대상으로 항균제 내성인자들을 지속적으로 조사하고 모니터링할 필요가 있을 것으로 사료된다.

• Journal of Species Research
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• 제10권2호
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• pp.99-116
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• 2021

In 2018, as a subset study to discover indigenous prokaryotic species in Korea, a total of 18 unreported bacterial strains were discovered. From the high 16S rRNA gene sequence similarity (>98.8%) and formation of a robust phylogenetic clade, it was determined that each strain belonged an independent and predefined bacterial species. There were no official report that these 18 species were previously described in Korea; therefore, one strain of Williamsia, one strain of Rhodococcus, three strains of Microbacterium, three strains of Agromyces, one strain of Arthrobacter, one strain of Paeniglutamicibacter, one strain of Pseudarthrobacter, one strain of Nocardioides, one strain of Fibrella, one strain of Hymenobacter, one strain of Deinococcus, two strains of Fictibacillus, and one strain of Paenibacillus are described as unreported bacterial species in Korea. Gram reaction, basic biochemical characteristics, and colony and cell morphologies are described in the species description section.

• Journal of Species Research
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• 제9권2호
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• pp.85-104
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• 2020

In 2019, after a comprehensive investigation of indigenous prokaryotic species in Korea, a total of 12 bacterial strains assigned to the phylum Proteobacteria were isolated from soil. With the high 16S rRNA gene sequence similarity (>98.8%) and formation of a robust phylogenetic clade with the closest species, it was determined that each strain belonged to independent, predefined bacterial species. This study identified two species in the family Burkholderiaceae, one species in the family Comamonadaceae, two species in the family Oxalobacteraceae, one species in the family Micrococcaceae, one species in the family Bradyrhizobiaceae, one species in the family Methylobacteriaceae, one species in the family Rhizobiaceae, one species in the family Rhodocyclaceae, and one species in the family Sphingomonadaceae. There is no official report about these 12 species in Korea, so are described as unreported bacterial species in Korea in this study. Gram reaction, basic biochemical characteristics, colony, and cell morphology are also described in the species description section.

• Animal Systematics, Evolution and Diversity
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• 제19권2호
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• pp.227-235
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• 2003

2002년 제주도의 한 웅덩이에서 채집된 섬모충류를 실험실에서 분리, 배양한 결과 하모류의 이끼토양자라하모충(Euplotes muscorum Dragesco, 1970)으로 동정되었으며 한국에서 처음으로 보고되어 재기재한다. 기재는 생체 표본 관찰, protargol impregnation 그리고 형태통계에 기초하여 기술하였다. 이 종의 형태적 특징은 몸길이 63-78${\mu}m$, 폭 40-52${\mu}m$, 9개의 전방복극모, 5개의 후방극모, 4개의 미극모, 한 개의 대핵과 소핵, 몸길이의 약 2/3을 덮고 있는 막판구대는 32-35개의 구부막판으로 이루어졌고, 8개의 등쪽섬모열, 중앙의 등쪽섬모열의 섬모는 20-24개, 등쪽섬모배열은 complex type 등의 특징들을 가진다. 이 종은 이끼자라하모충(E. muscicola Kahl,1932)과 매우 비슷하지만 이끼토양자라하모충은 8개의 등쪽섬모열, complex type의 등쪽섬모배열을 가지는데 반해 이끼자라하모충는 9개의 등쪽섬모열, multiple type의 등쪽섬모배열을 가지는 형태적 특징을 각각 나타낸다.

• 한국환경생태학회지
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• 제9권1호
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• pp.1-14
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• 1995

대산공단과 군산공단의 대기오염에 의한 삼림피해현상을 연구하기 위하여 조사구를 각각 5개, 3개씩 설치하였다(1개 조사구 당 면적 500$m^2$). 대산공단 삼림의 교목층 아교목층의 우점종은 소나무와 곰솔이었고 관목층에서는 진달래와 참나무류가 우점종이었다. 종다양도는 0.4859~0.9202 이었으며, 조사구간의 차이가 심하게 나타났다. 토양 pH는 4.67~5.12로 약산성이었다. 대산공단지역에서는 아직 대기오염에 의한 삼림의 피해가 심각하지 않은 것으로 나타났다. 군산공단삼림의 교목층 및 아교목층의 우점종은 곰솔과 소나무이었다. 종다양도는 0.8115~l.0983이었고 오염원과 가까운 조사구가 가장 낮게 나타났다. 토양 pH는 4.32~4.76으로 강산성이었다. 군산공단의 오염원 인근삼림은 울산공단과 유사한 피해양상으로 심각한 피해를 받고 있었다.

• 식물조직배양학회지
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• 제27권4호
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• pp.325-337
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• 2000

All living organisms depend on soil and water for their sustained growth and development. In recent years, sustenance of life in these growth matrices has been adversely affected by the cumulative increase in environmental pollutants resulting from increasing population, growing economies and resource-use. This review provides a glimpse into the problem of global environmental pollution, the traditional technologies available for remediation and the scope of emerging‘plant-based remediation’technologies. Phytoremediation, the use of plants to effectively remove or stabilize contaminants from the growth substrate, is a low cost and ecologically friendly alternative to the common‘dig and dump’technologies. The field of phytoremediation has been driven by the intrinsic need for identification of ideal candidate plant species. To date, there are only a very few identified plants which satisfy all of the prerequisites for use in phytoremediation. The review focuses on one such plant species, the common horticultural plant scented geranium (Pelargonium sp.), with demonstrated potential to remediate metal / salt contaminated soils / aqueous systems. The characterization of tolerance and metal / salt accumulation potential of Pelargonium sp. and its efficacy in remediating complex contaminated sites are described. The unique ability of scented geraniums to tolerate excessive amounts of multi-metals, hydrocarbon and salt mixtures, and at the same time to accumulate significant amounts of metal and salt ions in the biomass, renders this plant species as one of the ideal candidates for remediation.

• 한국자기공명학회논문지
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• 제5권1호
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• pp.1-12
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• 2001

ZSM-5 gallosilicate molecular sieves was synthesized and cupric ion was ion-exchanged into the gallosilicate. The locations of Cu(ll) species in the framework and their interactions with various adsorbates were characterized by combined electron spin resonance(ESR) and electron spin echo modulation(ESEM) methods. It was found that in a fresh hydrated material, Cu(II) is octahedrally coordinated to six water molecules. This species is located in the channel intersections of two sinusoidal channels and rotates rapidly at room temperature. Evacuation removes some of these water molecules, leaving the Cu(II) coordinated to less water molecules and anchored to of oxygens in the channel wall. Dehydration produces two Cu(II) species, both of which are located in sites inaccessible to oxygen as evidenced by non-broadening of its ESR lines by oxygen. Adsorption of adsorbate molecules such as water, alcohols, ammonia, acetonitrile and ethylene on dehydrated CuNaH-ZSM-5 gallosilicate materials causes changes in the ESR spectrum of Cu(II), indicating the migration of Cu(II) into main channels to form complexes with these adsorbates there. Cu(II) forms a complex with two molecules of methanol, ethanol and propanol, respectively as evidenced by ESR parameters and ESEM data. Cu(II) also forms a square planar complex with four molecules of ammonia, based on the resolved nitrogen superhyperfine interactions and their ESEM parameters. Cu(II) forms a complex with two molecules of acetonitrile based on the ESR parameters and ESEM data. Interestingly, however, only part of Cu(II) interacts indirectly with one molecule of nonpolar ethylene based on ESR and ESEM analyses.

• 한국균학회지
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• 제36권2호
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• pp.123-129
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• 2008

한국에서 해송의 줄기변색 부위에서 분리된 변색균류중 한 종에 대하여 그 특성을 조사하였다. Cycloheximide에 저항성인 Ophiostoma piceae complex 그룹에서 보여지는 무성세대 Petosum의 분생포자경과 또 다른 무성세대 Sporothrix의 형태를 지니고 있었다. 그러나 완전세대의 모습인 자낭각을 형성하지 못하였다. 이 균류는 배양적특성, 형태적특성 그리고 $\beta$-tubulin 유전자 염기서열 분석을 통하여 Ophiostoma quercus로 동정되었다. 본 연구에서는 한국에서 분리된 O. quercus의 균학적 특성을 처음으로 보고하고자 한다.